Dissimilarity-based representation for radiomics applications

Radiomics is a term which refers to the analysis of the large amount of quantitative tumor features extracted from medical images to find useful predictive, diagnostic or prognostic information. Many recent studies have proved that radiomics can offer a lot of useful information that physicians cannot extract from the medical images and can be associated with other information like gene or protein data. However, most of the classification studies in radiomics report the use of feature selection methods without identifying the machine learning challenges behind radiomics. In this paper, we first show that the radiomics problem should be viewed as an high dimensional, low sample size, multi view learning problem, then we compare different solutions proposed in multi view learning for classifying radiomics data. Our experiments, conducted on several real world multi view datasets, show that the intermediate integration methods work significantly better than filter and embedded feature selection methods commonly used in radiomics.Comment: conference, 6 pages, 2 figure

Dynamic voting in multi-view learning for radiomics applications

Cancer diagnosis and treatment often require a personalized analysis for each patient nowadays, due to the heterogeneity among the different types of tumor and among patients. Radiomics is a recent medical imaging field that has shown during the past few years to be promising for achieving this personalization. However, a recent study shows that most of the state-of-the-art works in Radiomics fail to identify this problem as a multi-view learning task and that multi-view learning techniques are generally more efficient. In this work, we propose to further investigate the potential of one family of multi-view learning methods based on Multiple Classifiers Systems where one classifier is learnt on each view and all classifiers are combined afterwards. In particular, we propose a random forest based dynamic weighted voting scheme, which personalizes the combination of views for each new patient for classification tasks. The proposed method is validated on several real-world Radiomics problems.Comment: 10 page

Démarche de conception d'une cellule de parachèvement

La fonderie d'aluminium de précision par moules en sable permet de réaliser des pièces complexes de grandes dimensions et de haute technicité. Cependant les exigences industrielles en terme de sécurité et de rentabilité remettent fortement en cause la réalisation manuelle des opérations de parachèvement. Cet article décrit tout d'abord la formalisation des différentes contraintes du process de parachèvement. Nous présentons ensuite différents aspects de la démarche de conception, analyse géométrique, étude d'accessibilité, précision, rigidité, qui a abouti à la définition d'une cellule robotisée 8 axes. Les travaux actuels portent sur la gestion optimisée de la redondance cinématique afin d'améliorer le comportement global de la cellule. Celle-ci est aujourd'hui opérationnelle et l'optimisation est en cours de validation sur des pièces industrielles. Ces travaux sont réalisés en partenariat avec la Société des Fonderies d'Ussel du groupe Alcan, spécialisée dans la réalisation de pièces de haute technicité pour les secteurs aéronautiques, transport et énergie

Review of the current status of RAS mutation testing in patients with metastatic colorectal cancer (mCRC): Flash-RAS study

Présentation PosterInternational audienceOBJECTIVES: In 2013, it was shown that mutations in KRAS exons 3 and 4, or NRAS exons 2 to 4 had a similar effect. The primary objective was to assess the practices in conducting RAS testing in 2014. The secondary objectives were to describe the evolution of the RAS testing prescription rates from 2011, the process and time required to obtain the results, and to analyze their impact on the therapeutic strategy. METHODS: FLASH-RAS is an observational retrospective French multicenter study. RESULTS: 375 mCRC patients diagnosed and initiating a 1st line treatment (L1) between March and June 2014 were analyzed. For 90.1% of the patients (IC95%= [87.1%; 93.2%]), a genotyping request for RAS biomarkers was made in L1, i.e. a significantly increased rate compared to 2011 (81.1% in 2011, p<0.001). For 75% of the patients, the request was made before or at least one month after the diagnosis of the first metastases (1st M). No increase was observed in the median and mean times to obtain the test results between 2011 and 2014 despite the increased number of exons tested. CONCLUSIONS: In 2014, the rate of RAS genotyping requests has been increasing since 2011. For a majority of patients, the request is made before or at the latest one month after 1st M diagnosis. Nevertheless, for 24.5% of the patients, the request is made more than one month after 1st M diagnosis, which is not compatible with an informed treatment decision in L1

Recent developments of marine ingredients for food and nutraceutical applications: a review.

Remerciements à l'éditeur pour son accord de diffusion. L'article original est aussi accessible sur le site de l'éditeur à l'adresse : http://www.halieutique.org/23201b.htmlInternational audienceIn a global context of marine biological resource overexploitation, a better upgrading of fish and shellfish biomass is a challenge for the 21st century. One of the main and promising issues will be the production of marine bio-ingredients using enzymatic hydrolysis. This paper presents the key steps in the production of enzymatic hydrolysates, such as (i) enzymatic treatment for the bioconversion of solid discards, and more particularly, use of proteases, (ii) quantification of the proteolysis extend and procedures of quality-control and (iii) identification of biological activity, using in vitro and in vivo methods. In the last part, examples of marine, commercially available functional foods or nutraceutical ingredients carrying bioactive properties are presented in order to demonstrate the interest of biotechnological exploitation of marine resources

FAK/src-Family Dependent Activation of the Ste20-Like Kinase SLK Is Required for Microtubule-Dependent Focal Adhesion Turnover and Cell Migration

Cell migration involves a multitude of signals that converge on cytoskeletal reorganization, essential for development, immune responses and tissue repair. Using knockdown and dominant negative approaches, we show that the microtubule-associated Ste20-like kinase SLK is required for focal adhesion turnover and cell migration downstream of the FAK/c-src complex. Our results show that SLK co-localizes with paxillin, Rac1 and the microtubules at the leading edge of migrating cells and is activated by scratch wounding. SLK activation is dependent on FAK/c-src/MAPK signaling, whereas SLK recruitment to the leading edge is src-dependent but FAK independent. Our results show that SLK represents a novel focal adhesion disassembly signal

Performance and cost efficiency of KRAS mutation testing for metastatic colorectal cancer in routine diagnosis: the MOKAECM study, a nationwide experience.

International audiencePURPOSE: Rapid advances in the understanding of cancer biology have transformed drug development thus leading to the approval of targeted therapies and to the development of molecular tests to select patients that will respond to treatments. KRAS status has emerged as a negative predictor of clinical benefit from anti-EGFR antibodies in colorectal cancer, and anti-EGFR antibodies use was limited to KRAS wild type tumors. In order to ensure wide access to tumor molecular profiling, the French National Cancer Institute (INCa) has set up a national network of 28 regional molecular genetics centers. Concurrently, a nationwide external quality assessment for KRAS testing (MOKAECM) was granted to analyze reproducibility and costs. METHODS: 96 cell-line DNAs and 24 DNA samples from paraffin embedded tumor tissues were sent to 40 French laboratories. A total of 5448 KRAS results were collected and analyzed and a micro-costing study was performed on sites for 5 common methods by an independent team of health economists. RESULTS: This work provided a baseline picture of the accuracy and reliability of KRAS analysis in routine testing conditions at a nationwide level. Inter-laboratory Kappa values were >0.8 for KRAS results despite differences detection methods and the use of in-house technologies. Specificity was excellent with only one false positive in 1128 FFPE data, and sensitivity was higher for targeted techniques as compared to Sanger sequencing based methods that were dependent upon local expertise. Estimated reagent costs per patient ranged from €5.5 to €19.0. CONCLUSION: The INCa has set-up a network of public laboratories dedicated to molecular oncology tests. Our results showed almost perfect agreements in KRAS testing at a nationwide level despite different testing methods ensuring a cost-effective equal access to personalized colorectal cancer treatment

Assessment of epidermal growth factor receptor (EGFR) expression in primary colorectal carcinomas and their related metastases on tissue sections and tissue microarray

Metastatic colorectal carcinomas (CRC) resistant to chemotherapy may benefit from targeting monoclonal therapy cetuximab when they express the epidermal growth factor receptor (EGFR). Because of its clinical implications, we studied EGFR expression by immunohistochemistry on tissue sections of primary CRC (n=32) and their related metastases (n=53). A tissue microarray (TMA) was generated from the same paraffin blocks to determine whether this technique could be used for EGFR screening in CRC. On tissue sections, 84% of the primary CRC and 94% of the metastases were EGFR-positive. When matched, they showed a concordant EGFR-positive status in 78% of the cases. Moreover, staining intensity and extent of EGFR-positive cells in the primary CRC correlated with those observed in the synchronous metastases. On TMA, 65% of the primary CRC, 66% of the metastases, and 43% of the matched primary CRC metastases were EGFR-positive. There was no concordant EGFR status between the primary and the metastatic sites. A strong discrepancy of EGFR status was noted between TMA and tissue sections. In conclusion, EGFR expression measured in tissue sections from primary CRC and their related metastases was found to be similar and frequent, but it was significantly underestimated by the TMA technique