EMPREGO DA COLA N-BUTIL CIANOACRILATO NA FIXAÇÃO DE RETALHO CUTÂNEO EM RATOS

O propósito do presente estudo foi avaliar clínica e histopatologicamente o uso da cola de n-butil cianoacrilato em procedimentos de retalho de avanço, uma vez que a mesma é freqüentemente utilizada em feridas pequenas com baixa tensão de pele. Foram utilizados 18 ratos, linhagem Wistar, divididos em dois grupos de nove. Após produzir-se uma ferida cutânea retangular de 2,0 x 1,5 cm, incluindo o músculo tronco cutâneo, na região torácica dorso-lateral esquerda, a mesma foi restaurada por meio de retalho cutâneo de pedículo único. No grupo I, o retalho foi fixado aplicando-se cola de n-butil cianoacrilato aplicada apenas na superfície interna das bordas e no grupo II realizaram-se pontos isolados com fio de náilon 4-0. Para avaliação histológica, três animais de cada grupo foram submetidos à eutanásia aos 07, 15 e 30 dias de pós-operatório.O tempo cirúrgico foi menor no grupo I, sendo a cola de fácil aplicação. Em ambos grupos observaram-se complicações, como inflamação e deiscência, porém sem diferenças estatísticas. O padrão cicatricial foi semelhante nos dois grupos. Foi possível concluir que a cola de n-butil cianoacrilato comportouse de forma semelhante à sutura, na síntese de retalhos cutâneos de pedículo único em ratos. Use of n-butyl cyanoacrylate glue with single pedicle advancement flap in rats Abstract The aim of this study was to evaluate clinically and histopathologically the use of nbutyl cyanoacrylate glue in advancement flap procedures, because this glue is usually used in small wounds with low skin tension. Eighteen Wistar rats with weight between 300 and 400 g were used and divided in two groups of nine each. A 2.0 x 1.5 cm rectangular skin wound, including the cutaneous trunci muscle, was performed in the left dorsal lateral thoracic side and repaired using the single pedicle advancement flap technique. In the first group, the flap was fixed with the glue on the internal surface of the edges. In the second group, the flap was fixed with simple interrupted suture using nylon 4-0. The surgical time was lower in group I, and glue application was easy. In both groups were observed complications, such as inflammation and wound dehiscence, but there was no statistical difference. Three animals from each group were euthanatized at seven, 15 and 30 days postoperative to realize the histological examination. It was possible to conclude that, macroscopically and microscopically, n-butyl cyanoacrylate glue was similar to suture in fixation of single pedicle advancement flap in rats

UTILIZAÇÃO DE PRÓPOLIS OU MEL NO TRATAMENTO DE FERIDAS LIMPAS INDUZIDAS EM RATOS

O trabalho objetivou verificar a influência do mel e do propólis na cicatrização de feridas limpas por segunda intenção, induzidos cirurgicamente. Foram utilizados 60 ratos, Wistar, fêmeas, com peso inicial entre 200 e 250 gramas, divididos em três grupos de vinte animais. Produziu-se uma ferida cutânea limpa na região torácica lateral esquerda e os animais foram submetidos aos seguintes tratamentos: grupo I própolis, grupo II mel e grupo III solução fisiológica 0,9% (controle). Com três, sete, 14 e 21 dias pós-operatórios, as feridas foram mensuradas e cinco ratos de cada grupo submetidos à eutanásia, para proceder exame histológico. A análise estatística das áreas das feridas não revelou diferenças significativas entre efeito de cada tratamento e número de dias após o tratamento. Histologicamente, os tratamentos com mel e própolis induziram melhor cicatrização pela redução da resposta inflamatória, havendo reepitelização mais rápida com o própolis. Use of propolis or honey in the treatment of clean wounds induced in rats Abstract Sixty female Wistar rats, initial weight from 200 to 250 g, were divided in three groups of twenty animals each. A clean skin wound was produced at the left lateral thoracic region. The wounds were treated with propolis (G1), honey (G2) and 0.9% saline solution (G3 control). Wounds were measured and five rats were euthanatized at 3, 7, 14 and 21 days postoperative to perform the histopathologic examination. There were no statistical differences among the effect of each treatment and number of days after the treatment. The histological examination showed that honey and propolis treatments induced better healing compared to saline solution due to the reduction of the inflammatory response, but the reepitelization was faster using propolis

HASTE INTRAMEDULAR DE POLIPROPILENO, COMBINADA OU NÃO A BIOMATERIAIS, NO TRATAMENTO DE FRATURAS INDUZIDAS NO ÚMERO DE POMBOS

O trabalho teve por objetivo avaliar a utilização da haste intramedular de polipropileno, combinada ou não a biomateriais de origem bovina, no tratamento de fraturas transversas induzidas no terço médio do úmero direito de pombos-domésticos. No grupo I (n=7), a fratura foi estabilizada por meio de haste intramedular de polipropileno aplicada em ambos os fragmentos da fratura, sem ultrapassar as articulações. No grupo II (n=7) utilizou-se a mesma fixação, porém foi aplicado junto ao foco de fratura uma mistura de biomateriais, constituída de proteínas morfogenéticas do osso e aglutinante de colágeno liofilizado. Em ambos os grupos, a asa foi imobilizada junto ao corpo com bandagem em forma de 8 por um período de 30 dias. Com seis semanas de pós-operatório, cinco fraturas do grupo I e quatro do grupo II estavam totalmente consolidadas. Foram observadas uma não consolidação hipertrófica (grupo I) e duas não consolidações com deslocamento do eixo ósseo (grupo II). Exceto as três aves que apresentaram complicações nas fraturas, as demais recuperaram a capacidade de vôo. Foi possível concluir que a haste intramedular de polipropileno foi um método de imobilização de eficácia limitada e os biomateriais utilizados não estimularam a consolidação das fraturas. Use of polypropylene intramedullary rod alone or combined with biomaterials for treatment of induced humeral transverse fractures in pigeons Abstract The aim of this study was to evaluate a polypropylene intramedullary rod alone or combined with biomaterials from bovine origin for the treatment of transverse fractures induced in the midshaft of right humerus in pigeons. In group I (n=7) the fracture was immobilized using a polypropylene intramedullary rod inserted into both fractured extremities without entering the joints. The same device was used in group II (n=7). Additionally, the fractured site in group II animals received a mixture of biomaterials composed by bone morphogenetic proteins and lyophilized collagen. In both groups a cast was applied to the affected wing, which was immobilized with a figure-of-8 bandage applied to the body and wing during 30 days. Complete healing was observed after six weeks of surgery in five and four animals from groups I and II, respectively. Observed complications included one hypertrophic nonunion (group I) and two nonunion with bone shaft dislocation (group II). Except for the three pigeons that showed postoperative complications, the remaining animals recovered the ability of flight. It was possible to conclude that the polypropylene intramedullary rod is a limited method of immobilization and the biomaterials used in this study did not stimulate fracture healing

Search for Neutral Higgs Bosons of the Minimal Supersymmetric Standard Model in e+e- Interactions at \sqrt{s} = 189 GeV

A search for the lightest neutral scalar and neutral pseudoscalar Higgs bosons in the Minimal Supersymmetric Standard Model is performed using 176.4 pb^-1 of integrated luminosity collected by L3 at a center-of-mass energy of 189 GeV. No signal is observed, and the data are consistent with the expected Standard Model background. Lower limits on the masses of the lightest neutral scalar and pseudoscalar Higgs bosons are given as a function of tan(beta). Lower mass limits for tan(beta)>1 are set at the 95% confidence level to be m_h > 77.1 GeV and m_A > 77.1 GeV

Measurement of the Probability of Gluon Splitting into Charmed Quarks in Hadronic Z Decays

We have measured the probability, n(g->cc~), of a gluon splitting into a charm-quark pair using 1.7 million hadronic Z decays collected by the L3 detector. Two independent methods have been applied to events with a three-jet topology. One method relies on tagging charmed hadrons by identifying a lepton in the lowest energy jet. The other method uses a neural network based on global event shape parameters. Combining both methods, we measure n(g->cc~)= [2.45 +/- 0.29 +/- 0.53]%

Search for Extra Dimensions in Boson and Fermion Pair Production in e+e- Interactions at LEP

Extra spatial dimensions are proposed by recent theories that postulate the scale of gravity to be of the same order as the electroweak scale. A sizeable interaction between gravitons and Standard Model particles is then predicted. Effects of these new interactions in boson and fermion pair production are searched for in the data sample collected at centre-of-mass energies above the Z pole by the L3 detector at LEP. In addition, the direct production of a graviton associated with a Z boson is investigated. No statistically significant hints for the existence of these effects are found and lower limits in excess of 1 TeV are derived on the scale of this new theory of gravity

Measurement of W Polarisation at LEP

The three different helicity states of W bosons produced in the reaction e+ e- -> W+ W- -> l nu q q~ at LEP are studied using leptonic and hadronic W decays. Data at centre-of-mass energies \sqrt s = 183-209 GeV are used to measure the polarisation of W bosons, and its dependence on the W boson production angle. The fraction of longitudinally polarised W bosons is measured to be 0.218 \pm 0.027 \pm 0.016 where the first uncertainty is statistical and the second systematic, in agreement with the Standard Model expectation

Measurement of Triple-Gauge-Boson Couplings of the W Boson at LEP

We report on measurements of the triple-gauge-boson couplings of the W boson in e+e- collisions with the L3 detector at LEP. W-pair, single-W and single-photon events are analysed in a data sample corresponding to a total luminosity of 76.7 pb^{-1} collected at centre-of-mass energies between 161 GeV and 183 GeV. CP-conserving as well as both C- and P-conserving triple-gauge-boson couplings are determined. The results, in good agreement with the Standard-Model expectations, confirm the existence of the self coupling among the electroweak gauge bosons and constrain its structure

Measurement of Hadron and Lepton-Pair Production at 130GeV < \sqrt{s} < 189 GeV at LEP

We report on measurements of e+e- annihilation into hadrons and lepton pairs. The data have been collected with the L3 detector at LEP at centre-of-mass energies between 130 and 189 GeV. Using a total integrated luminosity of 243.7 pb^-1, 25864 hadronic and 8573 lepton-pair events are selected for the measurement of cross sections and leptonic forward-backward asymmetries. The results are in good agreement with Standard Model predictions