1,569 research outputs found
Chlorinated auxins–how does Arabidopsis thaliana deal with them?
Plant hormones have various functions in plants and play crucial roles in all developmental and differentiation stages. Auxins constitute one of the most important groups with the major representative indole-3-acetic acid (IAA). A halogenated derivate of IAA, 4-chloro-indole-3-acetic acid (4-Cl-IAA), has previously been identified in Pisum sativum and other legumes. While the enzymes responsible for the halogenation of compounds in bacteria and fungi are well studied, the metabolic pathways leading to the production of 4-Cl-IAA in plants, especially the halogenating reaction, are still unknown. Therefore, bacterial flavin-dependent tryptophan-halogenase genes were transformed into the model organism Arabidopsis thaliana. The type of chlorinated indole derivatives that could be expected was determined by incubating wild type A. thaliana with different Cl-tryptophan derivatives. We showed that, in addition to chlorinated IAA, chlorinated IAA conjugates were synthesized. Concomitantly, we found that an auxin conjugate synthetase (GH3.3 protein) from A. thaliana was able to convert chlorinated IAAs to amino acid conjugates in vitro. In addition, we showed that the production of halogenated tryptophan (Trp), indole-3-acetonitrile (IAN) and IAA is possible in transgenic A. thaliana in planta with the help of the bacterial halogenating enzymes. Furthermore, it was investigated if there is an effect (i) of exogenously applied Cl-IAA and Cl-Trp and (ii) of endogenously chlorinated substances on the growth phenotype of the plants
Rest-frame ultra-violet spectra of massive galaxies at z=3: evidence of high-velocity outflows
Galaxy formation models invoke the presence of strong feedback mechanisms
that regulate the growth of massive galaxies at high redshifts. In this paper
we aim to: (1) confirm spectroscopically the redshifts of a sample of massive
galaxies selected with photometric redshifts z > 2.5; (2) investigate the
properties of their stellar and interstellar media; (3) detect the presence of
outflows, and measure their velocities. To achieve this, we analysed deep,
high-resolution (R~2000) FORS2 rest-frame UV spectra for 11 targets. We
confirmed that 9 out of 11 have spectroscopic redshifts z > 2.5. We also
serendipitously found two mask fillers at redshift z > 2.5, which originally
were assigned photometric redshifts 2.0 < z < 2.5. In the four highest-quality
spectra we derived outflow velocities by fitting the absorption line profiles
with models including multiple dynamical components. We found strongly
asymmetric, high-ionisation lines, from which we derived outflow velocities
ranging from 480 to 1518 km/s. The two galaxies with highest velocity show
signs of AGN. We revised the spectral energy distribution fitting U-band
through 8 micron photometry, including the analysis of a power-law component
subtraction to identify the possible presence of active galactic nuclei (AGN).
The revised stellar masses of all but one of our targets are >1e10 Msun, with
four having stellar masses > 5e10 Msun. Three galaxies have a significant
power-law component in their spectral energy distributions, which indicates
that they host AGN. We conclude that massive galaxies are characterised by
significantly higher velocity outflows than the typical Lyman break galaxies at
z ~ 3. The incidence of high-velocity outflows (~40% within our sample) is also
much higher than among massive galaxies at z < 1, which is consistent with the
powerful star formation and nuclear activity that most massive galaxies display
at z > 2.Comment: 17 pages, 14 figures, Accepted for publication in A&
Morphology and Redshifts of Extremely Red Galaxies in the GOODS/CDFS deep ISAAC field
We present the photometric redshift distribution of a sample of 198 Extremely
Red Galaxies (ERGs) with Ks3.92 (Vega), selected by Roche et al.
in 50.4 sq. arcmin of the Chandra Deep Field South (CDFS). The sample has been
obtained using ISAAC-VLT and ACS-HST GOODS public data. We also show the
results of a morphological study of the 72 brightest ERGs in the z band (z<25,
AB).Comment: 2 pages, 2 figures. To appear in the proceedings of the ESO/USM/MPE
Workshop "Multiwavelength Mapping of Galaxy Formation and Evolution", Venice,
October 13-16, 200
The role of the LIRG and ULIRG phases in the evolution of Ks-selected galaxies
We investigate the role of the luminous infrared galaxy (LIRG) and
ultra-luminous infrared galaxy (ULIRG) phases in the evolution of Ks-selected
galaxies and, in particular, Extremely Red Galaxies (ERGs). With this aim, we
compare the properties of a sample of 2905 Ks<21.5 (Vega mag) galaxies in the
GOODS/CDFS with the sub-sample of those 696 sources which are detected at 24
microns. We find that LIRGs constitute 30% of the galaxies with stellar mass
M>1x10^{11} Msun assembled at redshift z=0.5. A minimum of 65% of the galaxies
with M>2.5x10^{11} Msun at z~2-3 are ULIRGs at those redshifts. 60% of the
ULIRGs in our sample have the characteristic colours of ERGs. Conversely, 40%
of the ERGs with stellar mass M>1.3x10^{11} Msun at 1.5<z<2.0 and a minimum of
52% of those with the same mass cut at 2.0<z<3.0 are ULIRGs. The average
optical/near-IR properties of the massive ERGs at similar redshifts that are
identified with ULIRGs and that are not have basically no difference,
suggesting that both populations contain the same kind of objects in different
phases of their lives.
LIRGs and ULIRGs have an important role in galaxy evolution and mass
assembly, and, although they are only able to trace a fraction of the massive
(M>1x10^{11} Msun) galaxies present in the Universe at a given time, this
fraction becomes very significant (>50%) at redshifts z>~2.Comment: Accepted for publication in A&A. 9 pages, 6 figure
The stellar mass function of the most massive galaxies at 3<=z<5 in the UKIDSS Ultra Deep Survey
We have analysed a sample of 1292 4.5 micron-selected galaxies at z>=3, over
0.6 square degrees of the UKIRT Infrared Deep Survey (UKIDSS) Ultra Deep Survey
(UDS). Using photometry from the U band through 4.5 microns, we have obtained
photometric redshifts and derived stellar masses for our sources. Only two of
our galaxies potentially lie at z>5. We have studied the galaxy stellar mass
function at 3<=z<5, based on the 1213 galaxies in our catalogue with [4.5]<=
24.0. We find that: i) the number density of M > 10^11 Msun galaxies increased
by a factor > 10 between z=5 and 3, indicating that the assembly rate of these
galaxies proceeded > 20 times faster at these redshifts than at 0<z<2; ii) the
Schechter function slope alpha is significantly steeper than that displayed by
the local stellar mass function, which is both a consequence of the steeper
faint end and the absence of a pure exponential decline at the high-mass end;
iii) the evolution of the comoving stellar mass density from z=0 to 5 can be
modelled as log10 (rho_M) =-(0.05 +/- 0.09) z^2 - (0.22 -/+ 0.32) z + 8.69. At
3 10^11 Msun galaxies would be missed by optical
surveys with R<27 or z<26. Thus, our study demonstrates the importance of deep
mid-IR surveys over large areas to perform a complete census of massive
galaxies at high z and trace the early stages of massive galaxy assembly.Comment: 16 pages, 15 figures, 2 tables. Accepted for publication in MNRA
Combinatorial and topological aspects of path posets, and multipath cohomology
Multipath cohomology is a cohomology theory for directed graphs, which is defined using the path poset. The aim of this paper is to investigate combinatorial properties of path posets and to provide computational tools for multipath cohomology. In particular, we develop acyclicity criteria and provide computations of multipath cohomology groups of oriented linear graphs. We further interpret the path poset as the face poset of a simplicial complex, and we investigate realisability problems
Manual do Sistema Balanço HĂdrico e Calendário AgrĂcola.
Manual do Sistema Balanço HĂdrico; Explicação e observações sobre os termos empregados nas tabelas do Balanço HĂdrico; Modelo Entidade Relacionamento (MER); Objetivo operacional do sistema; Diferenciamento das duas maneiras de processamento do sistema; Funcionamento; MĂłdulo arquivos; MĂłdulo Balanço HĂdrico; MĂłdulo Utilitário; MĂłdulo Sobre.bitstream/item/116808/1/834.pd
The standardized Withania somnifera Dunal root extract alters basal and morphine-induced opioid receptor gene expression changes in neuroblastoma cells.
BACKGROUND:
Behavioral studies demonstrated that the administration of Withania somnifera Dunal roots extract (WSE), prolongs morphine-elicited analgesia and reduces the development of tolerance to the morphine's analgesic effect; however, little is known about the underpinning molecular mechanism(s). In order to shed light on this issue in the present paper we explored whether WSE promotes alterations of \u3bc (MOP) and nociceptin (NOP) opioid receptors gene expression in neuroblastoma SH-SY5Y cells.
METHODS:
A range of WSE concentrations was preliminarily tested to evaluate their effects on cell viability. Subsequently, the effects of 5 h exposure to WSE (0.25, 0.50 and 1.00 mg/ml), applied alone and in combination with morphine or naloxone, on MOP and NOP mRNA levels were investigated.
RESULTS:
Data analysis revealed that morphine decreased MOP and NOP receptor gene expression, whereas naloxone elicited their up-regulation. In addition, pre-treatment with naloxone prevented the morphine-elicited gene expression alterations. Interestingly, WSE was able to: a) alter MOP but not NOP gene expression; b) counteract, at its highest concentration, morphine-induced MOP down-regulation, and c) hamper naloxone-induced MOP and NOP up-regulation.
CONCLUSION:
Present in-vitro data disclose novel evidence about the ability of WSE to influence MOP and NOP opioid receptors gene expression in SH-SY5Y cells. Moreover, our findings suggest that the in-vivo modulation of morphine-mediated analgesia by WSE could be related to the hindering of morphine-elicited opioid receptors down-regulation here observed following WSE pre-treatment at its highest concentration
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