Bancos de dados como tecnologias habilitadoras para a agricultura de precisão: uma análise do contexto atual

Tecnologias de Agricultura de Precisão (AP) tem um papel fundamental no aumento da produtividade na agricultura moderna. Estas tecnologias têm como o aprimoramento do processo de tomada de decisão, o que gera a necessidade de coleta, armazenamento, processamento e análise de quantidades massivas de dados que crescem rapidamente. Assim, a escolha do Sistema de Gerenciamento de Banco de Dados (SGBD) é crucial para o sucesso destas tecnologias. Neste contexto, o presente trabalho apresenta uma revisão na literatura com a finalidade de verificar quais SGBD estão sendo utilizados em técnicas de AP atuais. Os resultados indicam que em aplicacões que geram grandes volumes de dados, como redes de sensores e imageamento digital, são preferidos os SGBD NoSQL em detrimento dos SGBD Relacionais convencionais.Sociedad Argentina de Informática e Investigación Operativ

Bancos de dados como tecnologias habilitadoras para a agricultura de precisão: uma análise do contexto atual

Tecnologias de Agricultura de Precisão (AP) tem um papel fundamental no aumento da produtividade na agricultura moderna. Estas tecnologias têm como o aprimoramento do processo de tomada de decisão, o que gera a necessidade de coleta, armazenamento, processamento e análise de quantidades massivas de dados que crescem rapidamente. Assim, a escolha do Sistema de Gerenciamento de Banco de Dados (SGBD) é crucial para o sucesso destas tecnologias. Neste contexto, o presente trabalho apresenta uma revisão na literatura com a finalidade de verificar quais SGBD estão sendo utilizados em técnicas de AP atuais. Os resultados indicam que em aplicacões que geram grandes volumes de dados, como redes de sensores e imageamento digital, são preferidos os SGBD NoSQL em detrimento dos SGBD Relacionais convencionais.Sociedad Argentina de Informática e Investigación Operativ

Congreso Argentino de Agroinformática: un análisis bibliometrico

Para viabilizar el intercambio de competencias y recursos, la colaboración en investigación se ha tornado fundamental para el progreso científico. Este trabajo presenta un abordaje para identificar y analizar la red de colaboración entre instituciones en el ámbito de la investigación en agroinformática en Argentina, a partir de los trabajos publicados en los anales de las tres últimas ediciones del Congreso Argentino de Agroinformática. La metodología se basó en la aplicación de técnicas Análisis de Redes Sociales, que permitieron identificar las instituciones más participativas en el contexto de la Agroinformática en Argentina, enfatizando la relevancia del Instituto Nacional de Tecnología Agropecuaria (INTA) y del Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) como catalizadores de la investigación en esta área. Se espera que los resultados aquí presentados puedan aportar elementos para ayudar a los organismos gubernamentales a establecer políticas para fortalecimiento de la investigación y desarrollo de la Agroinformática Argentina.Sociedad Argentina de Informática e Investigación Operativ

Congreso Argentino de Agroinformática: un análisis bibliometrico

Para viabilizar el intercambio de competencias y recursos, la colaboración en investigación se ha tornado fundamental para el progreso científico. Este trabajo presenta un abordaje para identificar y analizar la red de colaboración entre instituciones en el ámbito de la investigación en agroinformática en Argentina, a partir de los trabajos publicados en los anales de las tres últimas ediciones del Congreso Argentino de Agroinformática. La metodología se basó en la aplicación de técnicas Análisis de Redes Sociales, que permitieron identificar las instituciones más participativas en el contexto de la Agroinformática en Argentina, enfatizando la relevancia del Instituto Nacional de Tecnología Agropecuaria (INTA) y del Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) como catalizadores de la investigación en esta área. Se espera que los resultados aquí presentados puedan aportar elementos para ayudar a los organismos gubernamentales a establecer políticas para fortalecimiento de la investigación y desarrollo de la Agroinformática Argentina.Sociedad Argentina de Informática e Investigación Operativ

Congreso Argentino de Agroinformática: un análisis bibliometrico

Para viabilizar el intercambio de competencias y recursos, la colaboración en investigación se ha tornado fundamental para el progreso científico. Este trabajo presenta un abordaje para identificar y analizar la red de colaboración entre instituciones en el ámbito de la investigación en agroinformática en Argentina, a partir de los trabajos publicados en los anales de las tres últimas ediciones del Congreso Argentino de Agroinformática. La metodología se basó en la aplicación de técnicas Análisis de Redes Sociales, que permitieron identificar las instituciones más participativas en el contexto de la Agroinformática en Argentina, enfatizando la relevancia del Instituto Nacional de Tecnología Agropecuaria (INTA) y del Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) como catalizadores de la investigación en esta área. Se espera que los resultados aquí presentados puedan aportar elementos para ayudar a los organismos gubernamentales a establecer políticas para fortalecimiento de la investigación y desarrollo de la Agroinformática Argentina.Sociedad Argentina de Informática e Investigación Operativ

Modelo para Mensuração de Projetos de Circuitos Lógicos em Atividades Acadêmicas

Avaliando o tempo e a complexidade de circuitos, o artigo propõe uma métrica para análise de desempenho dos mesmos. O foco dessa pesquisa consiste em propor um modelo que sirva de auxílio ao aluno para concepção de um novo circuito e disponibilização de um feedback sobre a solução proposta

Identification of unannotated exons of low abundance transcripts in Drosophila melanogaster and cloning of a new serine protease gene upregulated upon injury

<p>Abstract</p> <p>Background</p> <p>The sequencing of the <it>D.melanogaster </it>genome revealed an unexpected small number of genes (~ 14,000) indicating that mechanisms acting on generation of transcript diversity must have played a major role in the evolution of complex metazoans. Among the most extensively used mechanisms that accounts for this diversity is alternative splicing. It is estimated that over 40% of <it>Drosophila </it>protein-coding genes contain one or more alternative exons. A recent transcription map of the <it>Drosophila </it>embryogenesis indicates that 30% of the transcribed regions are unannotated, and that 1/3 of this is estimated as missed or alternative exons of previously characterized protein-coding genes. Therefore, the identification of the variety of expressed transcripts depends on experimental data for its final validation and is continuously being performed using different approaches. We applied the Open Reading Frame Expressed Sequence Tags (ORESTES) methodology, which is capable of generating cDNA data from the central portion of rare transcripts, in order to investigate the presence of hitherto unnanotated regions of <it>Drosophila </it>transcriptome.</p> <p>Results</p> <p>Bioinformatic analysis of 1,303 <it>Drosophila </it>ORESTES clusters identified 68 sequences derived from unannotated regions in the current <it>Drosophila </it>genome version (4.3). Of these, a set of 38 was analysed by polyA⁺northern blot hybridization, validating 17 (50%) new exons of low abundance transcripts. For one of these ESTs, we obtained the cDNA encompassing the complete coding sequence of a new serine protease, named SP212. The <it>SP212 </it>gene is part of a serine protease gene cluster located in the chromosome region 88A12-B1. This cluster includes the predicted genes CG9631, CG9649 and CG31326, which were previously identified as up-regulated after immune challenges in genomic-scale microarray analysis. In agreement with the proposal that this <it>locus </it>is co-regulated in response to microorganisms infection, we show here that SP212 is also up-regulated upon injury.</p> <p>Conclusion</p> <p>Using the ORESTES methodology we identified 17 novel exons from low abundance <it>Drosophila </it>transcripts, and through a PCR approach the complete CDS of one of these transcripts was defined. Our results show that the computational identification and manual inspection are not sufficient to annotate a genome in the absence of experimentally derived data.</p

Dendritic Cells Transfected with scFv from Mab 7.B12 Mimicking Original Antigen gp43 Induces Protection against Experimental Paracoccidioidomycosis

Paracoccidioidomycosis (PCM), endemic in Latin America, is a progressive systemic mycosis caused by Paracoccidioides brasiliensis (P. brasiliensis), which primarily attacks lung tissue. Dendritic cells (DCs) are able to initiate a response in naïve T cells, and they also participate in Th-cell education. Furthermore, these cells have been used for therapy in several disease models. Here we transfected DCs with a plasmid (pMAC/PS-scFv) encoding a single chain variable fragment (scFv) of an anti-Id antibody that is capable of mimicking gp43, the main antigenic component of P. brasiliensis. First, Balb/c mice were immunized subcutaneously with pMAC/PS-scFv and, after seven days, scFv protein was presented to the regional lymph nodes cells. Moreover, we showed that the DCs transfected with scFv were capable of efficiently activating proliferation of total lymph node cells and inducing a decrease in lung infection. Therefore, our results suggested that the use of scFv-transfected DCs may be a promising therapy in the paracoccidioidomycosis (PCM) model

The complete genome sequence of Chromobacterium violaceum reveals remarkable and exploitable bacterial adaptability

Chromobacterium violaceum is one of millions of species of free-living microorganisms that populate the soil and water in the extant areas of tropical biodiversity around the world. Its complete genome sequence reveals (i) extensive alternative pathways for energy generation, (ii) ≈500 ORFs for transport-related proteins, (iii) complex and extensive systems for stress adaptation and motility, and (iv) wide-spread utilization of quorum sensing for control of inducible systems, all of which underpin the versatility and adaptability of the organism. The genome also contains extensive but incomplete arrays of ORFs coding for proteins associated with mammalian pathogenicity, possibly involved in the occasional but often fatal cases of human C. violaceum infection. There is, in addition, a series of previously unknown but important enzymes and secondary metabolites including paraquat-inducible proteins, drug and heavy-metal-resistance proteins, multiple chitinases, and proteins for the detoxification of xenobiotics that may have biotechnological applications

Differences in Cell Morphometry, Cell Wall Topography and Gp70 Expression Correlate with the Virulence of <i>Sporothrix brasiliensis</i> Clinical Isolates

<div><p>Sporotrichosis is a chronic infectious disease affecting both humans and animals. For many years, this subcutaneous mycosis had been attributed to a single etiological agent; however, it is now known that this taxon consists of a complex of at least four pathogenic species, including <i>Sporothrix schenckii</i> and <i>Sporothrix brasiliensis</i>. Gp70 was previously shown to be an important antigen and adhesin expressed on the fungal cell surface and may have a key role in immunomodulation and host response. The aim of this work was to study the virulence, morphometry, cell surface topology and gp70 expression of clinical isolates of <i>S. brasiliensis</i> compared with two reference strains of <i>S. schenckii</i>. Several clinical isolates related to severe human cases or associated with the Brazilian zoonotic outbreak of sporotrichosis were genotyped and clustered as <i>S. brasiliensis</i>. Interestingly, in a murine subcutaneous model of sporotrichosis, these isolates showed a higher virulence profile compared with <i>S. schenckii</i>. A single <i>S. brasiliensis</i> isolate from an HIV-positive patient not only showed lower virulence but also presented differences in cell morphometry, cell wall topography and abundant gp70 expression compared with the virulent isolates. In contrast, the highly virulent <i>S. brasiliensis</i> isolates showed reduced levels of cell wall gp70. These observations were confirmed by the topographical location of the gp70 antigen using immunoelectromicroscopy in both species. In addition, the gp70 molecule was sequenced and identified using mass spectrometry, and the sequenced peptides were aligned into predicted proteins using Blastp with the <i>S. schenckii</i> and <i>S. brasiliensis</i> genomes<i>.</i></p> </div