Extending periodic eddy covariance latent heat fluxes through tree sap-flow measurements to estimate long-term total evaporation in a peat swamp forest

A combination of measurement and modelling was used to find a pragmatic solution to estimate the annual total evaporation from the rare and indigenous Nkazana Peat Swamp Forest (PSF) on the east coast of Southern Africa to improve the water balance estimates within the area. Actual total evaporation (ETa) was measured during three window periods (between 7 and 9 days each) using an eddy covariance (EC) system on a telescopic mast above the forest canopy. Sap flows of an understory tree and an emergent tree were measured using a low-maintenance heat pulse velocity system for an entire hydrological year (October 2009 to September 2010). An empirical model was derived, describing the relationship between ETa from the Nkazana PSF and sap-flow measurements. These overlapped during two of the window periods (R2 = 0.92 and 0.90), providing hourly estimates of ETa from the Nkazana PSF for a year, totalling 1125 mm (while rainfall was 650 mm). In building the empirical model, it was found that to include the understory tree sap flow provided no benefit to the model performance. In addition, the relationship between the emergent tree sap flow with ETa between the two field campaigns was consistent and could be represented by a single empirical model (R2 = 0.90; RMSE = 0.08 mm h−1). During the window periods of EC measurement, no single meteorological variable was found to describe the Nkazana PSF ETa satisfactorily. However, in terms of evaporation models, the hourly FAO Penman–Monteith reference evaporation (ETo) best described ETa during the August 2009 (R2 = 0.75), November 2009 (R2 = 0.85) and March 2010 (R2 = 0.76) field campaigns, compared to the Priestley–Taylor potential evaporation (ETp) model (R2 = 0.54, 0.74 and 0.62 during the respective field campaigns). From the extended record of ETa (derived in this study from sap flow) and ETo, a monthly crop factor (Kc) was derived for the Nkazana PSF, providing a method of estimating long-term swamp forest water-use from meteorological data. The monthly Kc indicated two distinct periods. From February to May, it was between 1.2 and 1.4 compared with June to January, when the crop factor was 0.8 to 1.0. The derived monthly Kc values were verified as accurate (to one significant digit) using historical data measured at the same site, also using EC, from a previous study. The measurements provided insights into the microclimate within a subtropical peat swamp forest and the contrasting sap flow of emergent and understory trees. They showed that expensive, high-maintenance equipment can be used during manageable window periods in conjunction with low-maintenance systems, dedicated to individual trees, to derive a model to estimate long-term ETa over remote heterogeneous forests. In addition, the contrast in annual ETa and rainfall emphasised the reliance of the Nkazana PSF on groundwater

A new method to measure Bowen ratios using high-resolution vertical dry and wet bulb temperature profiles

The Bowen ratio surface energy balance method is a relatively simple method to determine the latent heat flux and the actual land surface evaporation. The Bowen ratio method is based on the measurement of air temperature and vapour pressure gradients. If these measurements are performed at only two heights, correctness of data becomes critical. In this paper we present the concept of a new measurement method to estimate the Bowen ratio based on vertical dry and wet bulb temperature profiles with high spatial resolution. A short field experiment with distributed temperature sensing (DTS) in a fibre optic cable with 13 measurement points in the vertical was undertaken. A dry and a wetted section of a fibre optic cable were suspended on a 6 m high tower installed over a sugar beet trial plot near Pietermaritzburg (South Africa). Using the DTS cable as a psychrometer, a near continuous observation of vapour pressure and air temperature at 0.20 m intervals was established. These data allowed the computation of the Bowen ratio with a high spatial and temporal precision. The daytime latent and sensible heat fluxes were estimated by combining the Bowen ratio values from the DTS-based system with independent measurements of net radiation and soil heat flux. The sensible heat flux, which is the relevant term to evaluate, derived from the DTS-based Bowen ratio (BR-DTS) was compared with that derived from co-located eddy covariance (<i>R</i>² = 0.91), surface layer scintillometer (<i>R</i>² = 0.81) and surface renewal (<i>R</i>² = 0.86) systems. By using multiple measurement points instead of two, more confidence in the derived Bowen ratio values is obtained

New weapons in the toad toolkit: A review of methods to control and mitigate the biodiversity impacts of invasive cane toads (rhinella marina)

© 2017 by The University of Chicago Press. All rights reserved. Our best hope of developing innovative methods to combat invasive species is likely to come from the study of high-profile invaders that have attracted intensive research not only into control, but also basic biology. Here we illustrate that point by reviewing current thinking about novel ways to control one of the world’s most well-studied invasions: that of the cane toad in Australia. Recently developed methods for population suppression include more effective traps based on the toad’s acoustic and pheromonal biology. New tools for containing spread include surveillance technologies (e.g., eDNA sampling and automated call detectors), as well as landscape-level barriers that exploit the toad’s vulnerability to desiccation— a strategy that could be significantly enhanced through the introduction of sedentary, rangecore genotypes ahead of the invasion front. New methods to reduce the ecological impacts of toads include conditioned taste aversion in free-ranging predators, gene banking, and targeted gene flow. Lastly, recent advances in gene editing and gene drive technology hold the promise of modifying toad phenotypes in ways that may facilitate control or buffer impact. Synergies between these approaches hold great promise for novel and more effective means to combat the toad invasion and its consequent impacts on biodiversity

Identification of genes differentially expressed in a resistant reaction to Mycosphaerella pinodes in pea using microarray technology

<p>Abstract</p> <p>Background</p> <p>Ascochyta blight, caused by <it>Mycosphaerella pinodes </it>is one of the most important pea pathogens. However, little is known about the genes and mechanisms of resistance acting against <it>M. pinodes </it>in pea. Resistance identified so far to this pathogen is incomplete, polygenic and scarce in pea, being most common in <it>Pisum </it>relatives. The identification of the genes underlying resistance would increase our knowledge about <it>M. pinodes-</it>pea interaction and would facilitate the introgression of resistance into pea varieties. In the present study differentially expressed genes in the resistant <it>P. sativum </it>ssp. <it>syriacum </it>accession P665 comparing to the susceptible pea cv. Messire after inoculation with <it>M. pinodes </it>have been identified using a <it>M. truncatula </it>microarray.</p> <p>Results</p> <p>Of the 16,470 sequences analysed, 346 were differentially regulated. Differentially regulated genes belonged to almost all functional categories and included genes involved in defense such as genes involved in cell wall reinforcement, phenylpropanoid and phytoalexins metabolism, pathogenesis- related (PR) proteins and detoxification processes. Genes associated with jasmonic acid (JA) and ethylene signal transduction pathways were induced suggesting that the response to <it>M. pinodes </it>in pea is regulated via JA and ET pathways. Expression levels of ten differentially regulated genes were validated in inoculated and control plants using qRT-PCR showing that the P665 accession shows constitutively an increased expression of the defense related genes as peroxidases, disease resistance response protein 39 (DRR230-b), glutathione S-transferase (GST) and 6a-hydroxymaackiain methyltransferase.</p> <p>Conclusions</p> <p>Through this study a global view of genes expressed during resistance to <it>M. pinodes </it>has been obtained, giving relevant information about the mechanisms and pathways conferring resistance to this important disease. In addition, the <it>M. truncatula </it>microarray represents an efficient tool to identify candidate genes controlling resistance to <it>M. pinodes </it>in pea.</p

On the Security of Carrier Phase-based Ranging

Multicarrier phase-based ranging is fast emerging as a cost-optimized solution for a wide variety of proximity-based applications due to its low power requirement, low hardware complexity and compatibility with existing standards such as ZigBee and 6LoWPAN. Given potentially critical nature of the applications in which phase-based ranging can be deployed (e.g., access control, asset tracking), it is important to evaluate its security guarantees. Therefore, in this work, we investigate the security of multicarrier phase-based ranging systems and specifically focus on distance decreasing relay attacks that have proven detrimental to the security of proximity-based access control systems (e.g., vehicular passive keyless entry and start systems). We show that phase-based ranging, as well as its implementations, are vulnerable to a variety of distance reduction attacks. We describe different attack realizations and verify their feasibility by simulations and experiments on a commercial ranging system. Specifically, we successfully reduced the estimated range to less than 3 m even though the devices were more than 50 m apart. We discuss possible countermeasures against such attacks and illustrate their limitations, therefore demonstrating that phase-based ranging cannot be fully secured against distance decreasing attacks

Deconstructing compassionate conservation

Compassionate conservation focuses on 4 tenets: first, do no harm; individuals matter; inclusivity of individual animals; and peaceful coexistence between humans and animals. Recently, compassionate conservation has been promoted as an alternative to conventional conservation philosophy. We believe examples presented by compassionate conservationists are deliberately or arbitrarily chosen to focus on mammals; inherently not compassionate; and offer ineffective conservation solutions. Compassionate conservation arbitrarily focuses on charismatic species, notably large predators and megaherbivores. The philosophy is not compassionate when it leaves invasive predators in the environment to cause harm to vastly more individuals of native species or uses the fear of harm by apex predators to terrorize mesopredators. Hindering the control of exotic species (megafauna, predators) in situ will not improve the conservation condition of the majority of biodiversity. The positions taken by so-called compassionate conservationists on particular species and on conservation actions could be extended to hinder other forms of conservation, including translocations, conservation fencing, and fertility control. Animal welfare is incredibly important to conservation, but ironically compassionate conservation does not offer the best welfare outcomes to animals and is often ineffective in achieving conservation goals. Consequently, compassionate conservation may threaten public and governmental support for conservation because of the limited understanding of conservation problems by the general public

Unambiguous detection of nitrated explosive vapours by fluorescence quenching of dendrimer films

Unambiguous and selective standoff (non-contact) infield detection of nitro-containingexplosives and taggants is an important goal but difficult to achieve with standard analyticaltechniques. Oxidative fluorescence quenching is emerging as a high sensitivity method fordetecting such materials but is prone to false positives—everyday items such as perfumeselicit similar responses. Here we report thin films of light-emitting dendrimers that detectvapours of explosives and taggants selectively—fluorescence quenching is not observed for arange of common interferents. Using a combination of neutron reflectometry, quartz crystalmicrobalance and photophysical measurements we show that the origin of the selectivity isprimarily electronic and not the diffusion kinetics of the analyte or its distribution in the film.The results are a major advance in the development of sensing materials for the standoffdetection of nitro-based explosive vapours, and deliver significant insights into the physicalprocesses that govern the sensing efficacy

Red hot frogs:Identifying the Australian frogs most at risk of extinction

More than a third of the world’s amphibian species are listed as Threatened or Extinct, with a recent assessment identifying 45 Australian frogs (18.4% of the currently recognised species) as ‘Threatened’ based on IUCN criteria. We applied structured expert elicitation to 26 frogs assessed as Critically Endangered and Endangered to estimate their probability of extinction by 2040. We also investigated whether participant experience (measured as a self-assigned categorical score, i.e. ‘expert’ or ‘non-expert’) influenced the estimates. Collation and analysis of participant opinion indicated that eight species are at high risk (>50% chance) of becoming extinct by 2040, with the disease chytridiomycosis identified as the primary threat. A further five species are at moderate–high risk (30–50% chance), primarily due to climate change. Fourteen of the 26 frog species are endemic to Queensland, with many species restricted to small geographic ranges that are susceptible to stochastic events (e.g. a severe heatwave or a large bushfire). Experts were more likely to rate extinction probability higher for poorly known species (those with <10 experts), while non-experts were more likely to rate extinction probability higher for better-known species. However, scores converged following discussion, indicating that there was greater consensus in the estimates of extinction probability. Increased resourcing and management intervention are urgently needed to avert future extinctions of Australia’s frogs. Key priorities include developing and supporting captive management and establishing or extending in-situ population refuges to alleviate the impacts of disease and climate change

Genetic mechanisms of critical illness in COVID-19.

Host-mediated lung inflammation is present1, and drives mortality2, in the critical illness caused by coronavirus disease 2019 (COVID-19). Host genetic variants associated with critical illness may identify mechanistic targets for therapeutic development3. Here we report the results of the GenOMICC (Genetics Of Mortality In Critical Care) genome-wide association study in 2,244 critically ill patients with COVID-19 from 208 UK intensive care units. We have identified and replicated the following new genome-wide significant associations: on chromosome 12q24.13 (rs10735079, P = 1.65 × 10-8) in a gene cluster that encodes antiviral restriction enzyme activators (OAS1, OAS2 and OAS3); on chromosome 19p13.2 (rs74956615, P = 2.3 × 10-8) near the gene that encodes tyrosine kinase 2 (TYK2); on chromosome 19p13.3 (rs2109069, P = 3.98 ×  10-12) within the gene that encodes dipeptidyl peptidase 9 (DPP9); and on chromosome 21q22.1 (rs2236757, P = 4.99 × 10-8) in the interferon receptor gene IFNAR2. We identified potential targets for repurposing of licensed medications: using Mendelian randomization, we found evidence that low expression of IFNAR2, or high expression of TYK2, are associated with life-threatening disease; and transcriptome-wide association in lung tissue revealed that high expression of the monocyte-macrophage chemotactic receptor CCR2 is associated with severe COVID-19. Our results identify robust genetic signals relating to key host antiviral defence mechanisms and mediators of inflammatory organ damage in COVID-19. Both mechanisms may be amenable to targeted treatment with existing drugs. However, large-scale randomized clinical trials will be essential before any change to clinical practice