Plant growth responses to elevated atmospheric CO2 are increased by phosphorus sufficiency but not by arbuscular mycorrhizas

Capturing the full growth potential in crops under future elevated CO₂ (eCO₂) concentrations would be facilitated by improved understanding of eCO₂ effects on uptake and use of mineral nutrients. This study investigates interactions of eCO₂, soil phosphorus (P), and arbuscular mycorrhizal (AM) symbiosis in Medicago truncatula and Brachypodium distachyon grown under the same conditions. The focus was on eCO₂ effects on vegetative growth, efficiency in acquisition and use of P, and expression of phosphate transporter (PT) genes. Growth responses to eCO₂ were positive at P sufficiency, but under low-P conditions they ranged from non-significant in M. truncatula to highly significant in B. distachyon Growth of M. truncatula was increased by AM at low P conditions at both CO₂ levels and eCO₂×AM interactions were sparse. Elevated CO₂ had small effects on P acquisition, but enhanced conversion of tissue P into biomass. Expression of PT genes was influenced by eCO₂, but effects were inconsistent across genes and species. The ability of eCO₂ to partly mitigate P limitation-induced growth reductions in B. distachyon was associated with enhanced P use efficiency, and requirements for P fertilizers may not increase in such species in future CO₂-rich climates.Iver Jakobsen, Sally E. Smith, F. Andrew Smith, Stephanie J. Watts-Williams, Signe S. Clausen and Mette Grønlun

Nutrient interactions and arbuscular mycorrhizas: a meta-analysis of a mycorrhiza-defective mutant and wild-type tomato genotype pair

BACKGROUND AND AIMS Arbuscular mycorrhizas (AM) enhance plant uptake of a range of mineral nutrients from the soil. Interactions between nutrients in the soil and plant, are complex, and can be affected by AM. Using a mycorrhiza-defective mutant tomato genotype (rmc) and its wild-type (76R), provides a novel method to study AM functioning. METHODS We present a meta-analysis comparing tissue nutrient concentration (P, Zn, K, Ca, Cu, Mg, Mn, S, B, Na, Fe), biomass and mycorrhizal colonisation data between the 76R and rmc genotypes, across a number of studies that have used this pair of tomato genotypes. Particular attention is paid to interactions between soil P or soil Zn, with tissue nutrients. RESULTS For most nutrients, the difference in concentration between genotypes was significantly affected either by soil P, soil Zn, or both. When soil P was deficient, AM were particularly beneficial in terms of uptake of not only P, but other nutrients as well. CONCLUSIONS Colonisation by AMF significantly affects the uptake of many soil macro- and micro-nutrients. Furthermore, the soil P and Zn status also influences the difference in nutrient concentrations between mycorrhizal and non-mycorrhizal plants. The interactions identified by this meta-analysis provide a basis for future research in this area.Stephanie J. Watts-Williams, Timothy R. Cavagnar

Using mycorrhiza-defective mutant genotypes of non-legume plant species to study the formation and functioning of arbuscular mycorrhiza: a review

A significant challenge facing the study of arbuscular mycorrhiza is the establishment of suitable non-mycorrhizal treatments that can be compared with mycorrhizal treatments. A number of options are available, including soil disinfection or sterilisation, comparison of constitutively mycorrhizal and non-mycorrhizal plant species, comparison of plants grown in soils with different inoculum potential and the comparison of mycorrhiza-defective mutant genotypes with their mycorrhizal wild-type progenitors. Each option has its inherent advantages and limitations. Here, the potential to use mycorrhiza-defective mutant and wild-type genotype plant pairs as tools to study the functioning of mycorrhiza is reviewed. The emphasis of this review is placed on non-legume plant species, as mycorrhiza-defective plant genotypes in legumes have recently been extensively reviewed. It is concluded that non-legume mycorrhiza-defective mutant and wild-type pairs are useful tools in the study of mycorrhiza. However, the mutant genotypes should be well characterised and, ideally, meet a number of key criteria. The generation of more mycorrhiza-defective mutant genotypes in agronomically important plant species would be of benefit, as would be more research using these genotype pairs, especially under field conditions.Stephanie J. Watts-Williams, Timothy R. Cavagnar

A Glass of Milk: The Rocha, Nicaragua Goat Project

This panel will talk about their plans to embark on a goat-gifting project in two rural Nicaraguan communities

Solution of the Unanimity Rule on exponential, uniform and scalefree networks: A simple model for biodiversity collapse in foodwebs

We solve the Unanimity Rule on networks with exponential, uniform and scalefree degree distributions. In particular we arrive at equations relating the asymptotic number of nodes in one of two states to the initial fraction of nodes in this state. The solutions for exponential and uniform networks are exact, the approximation for the scalefree case is in perfect agreement with simulation results. We use these solutions to provide a theoretical understanding for experimental data on biodiversity loss in foodwebs, which is available for the three network types discussed. The model allows in principle to estimate the critical value of species that have to be removed from the system to induce its complete collapse.Comment: 4 pages, 3 fig

Statistical Analysis of Airport Network of China

Through the study of airport network of China (ANC), composed of 128 airports (nodes) and 1165 flights (edges), we show the topological structure of ANC conveys two characteristics of small worlds, a short average path length (2.067) and a high degree of clustering (0.733). The cumulative degree distributions of both directed and undirected ANC obey two-regime power laws with different exponents, i.e., the so-called Double Pareto Law. In-degrees and out-degrees of each airport have positive correlations, whereas the undirected degrees of adjacent airports have significant linear anticorrelations. It is demonstrated both weekly and daily cumulative distributions of flight weights (frequencies) of ANC have power-law tails. Besides, the weight of any given flight is proportional to the degrees of both airports at the two ends of that flight. It is also shown the diameter of each sub-cluster (consisting of an airport and all those airports to which it is linked) is inversely proportional to its density of connectivity. Efficiency of ANC and of its sub-clusters are measured through a simple definition. In terms of that, the efficiency of ANC's sub-clusters increases as the density of connectivity does. ANC is found to have an efficiency of 0.484.Comment: 6 Pages, 5 figure

Growing Scale-Free Networks with Small World Behavior

In the context of growing networks, we introduce a simple dynamical model that unifies the generic features of real networks: scale-free distribution of degree and the small world effect. While the average shortest path length increases logartihmically as in random networks, the clustering coefficient assumes a large value independent of system size. We derive expressions for the clustering coefficient in two limiting cases: random (C ~ (ln N)^2 / N) and highly clustered (C = 5/6) scale-free networks.Comment: 4 pages, 4 figure

Subgraph Centrality in Complex Networks

We introduce a new centrality measure that characterizes the participation of each node in all subgraphs in a network. Smaller subgraphs are given more weight than larger ones, which makes this measure appropriate for characterizing network motifs. We show that the subgraph centrality (SC) can be obtained mathematically from the spectra of the adjacency matrix of the network. This measure is better able to discriminate the nodes of a network than alternate measures such as degree, closeness, betweenness and eigenvector centralities. We study eight real-world networks for which SC displays useful and desirable properties, such as clear ranking of nodes and scale-free characteristics. Compared with the number of links per node, the ranking introduced by SC (for the nodes in the protein interaction network of S. cereviciae) is more highly correlated with the lethality of individual proteins removed from the proteome.Comment: 29 pages, 4 figures, 2 table

Analytical solution of a model for complex food webs

We investigate numerically and analytically a recently proposed model for food webs [Nature {\bf 404}, 180 (2000)] in the limit of large web sizes and sparse interaction matrices. We obtain analytical expressions for several quantities with ecological interest, in particular the probability distributions for the number of prey and the number of predators. We find that these distributions have fast-decaying exponential and Gaussian tails, respectively. We also find that our analytical expressions are robust to changes in the details of the model.Comment: 4 pages (RevTeX). Final versio

Uptake of zinc and phosphorus by plants is affected by zinc fertiliser material and arbuscular mycorrhizas

Background and Aims Water solubility of zinc (Zn) fertilisers affects their plant availability. Further, simultaneous application of Zn and phosphorus (P) fertiliser can have antagonistic effects on plant Zn uptake. Arbuscular mycorrhizas (AM) can improve plant Zn and P uptake. We conducted a glasshouse experiment to test the effect of different Zn fertiliser materials, in conjunction with P fertiliser application, and colonisation by AM, on plant nutrition and biomass. Methods We grew a mycorrhiza-defective tomato geno-type (rmc) and its mycorrhizal wild-type progenitor(76R) in soil with six different Zn fertilisers ranging in water solubility (Zn sulphate, Zn oxide, Zn oxide (nano), Zn phosphate, Zn carbonate, Zn phosphate carbonate), and supplemental P. We measured plant biomass, Zn and P contents, mycorrhizal colonisation and water use efficiency. Results Whereas water solubility of the Zn fertilisers was not correlated with plant biomass or Zn uptake, plant Zn and P contents differed among Zn fertiliser treatments. Plant Zn and P uptake was enhanced when supplied as Zn phosphate carbonate. Mycorrhizal plants took up more P than non-mycorrhizal plants; the reverse was true for Zn. Conclusions Zinc fertiliser composition and AM have a profound effect on plant Zn and P uptake.Stephanie J. Watts-Williams, Terence W. Turney, Antonio F. Patti, Timothy R. Cavagnar