Hot Wire Anemometer

A hot wire anemometer circuit has a calibrate subcircuit and an operate subcircuit which are selectively invoked via a user-activate mode switch. The calibrate subcircuit includes a detector configured to compare an amplified sensor voltage with an amplified drive voltage and output an indicator signal when the two voltages match. The drive voltage is derived from a user-adjusted drive resistance of a bridge subcircuit. The operate circuit includes a feedback loop which provides a signal to a bride node of a bridge circuit. After a sensor is connected to the anemometer circuit, the drive resistance is adjusted until the indicator signal is produced, signifying that the circuit is tuned and impedances are matched to prevent oscillations. A slewing network protects the sensor by short­ing the drive resistance upon switching the anemometer cir­cuit to the calibrate mode from the operate mode, and gradu­ally restoring the drive resistance when switching back

Hot Wire Anemoteter

A hot wire anemometer circuit has a calibrate subcircuit and an operate subcircuit which are selectively invoked via a user-activate mode switch. The calibrate subcircuit includes a detector configured to compare an amplified sensor voltage with an amplified drive voltage and output an indicator signal when the two voltages match. The drive voltage is derived from a user-adjusted drive resistance of a bridge subcircuit. The operate circuit includes a feedback loop which provides a signal to a bride node of a bridge circuit. After a sensor is connected to the anemometer circuit, the drive resistance is adjusted until the indicator signal is produced, signifying that the circuit is tuned and impedances are matched to prevent oscillations. A slewing network protects the sensor by shorting the drive resistance upon switching the anemometer circuit to the calibrate mode from the operate mode, and gradually restoring the drive resis­tance when switching back

Art Therapy ethical practice and multicultural/diversity competence

The Conference proceedings' website is located at http://www.xcdsystem.com/aata2013/proceedings/prof108.htmlTheme: Art Therapy: Connecting Visual Expression & HealingThe United States Census (2010) confirms a shift from a nation that is primarily White, from Western European backgrounds, to a country in which the majority of residents are from visible racial ethnic, and non-English speaking backgrounds (D’Andrea & Arrendondo, 2002). Consequently, this shift implies art therapists will be challenged to become expert in culturally congruent treatment. The American Art Therapy Association’s Ethical Principles for Art Therapists (2011) Section 6.0 serves …postprin

Happiness on Tap: Piped Water Adoption in Urban Morocco

We study the demand for household water connections in urban Morocco, and the effect of such connections on household welfare. In the northern city of Tangiers, among homeowners without a private connection to the city’s water grid, a random subset was offered a simplified procedure to purchase a household connection on credit (at a zero percent interest rate). Take-up was high, at 69%. Because all households in our sample had access to the water grid through free public taps (often located fairly close to their homes), household connections did not lead to any improvement in the quality of the water households consumed; and despite significant increase in the quantity of water consumed, we find no change in the incidence of waterborne illnesses. Nevertheless, we find that households are willing to pay a substantial amount of money to have a private tap at home. Being connected generates important time gains, which are used for leisure and social activities, rather than productive activities. Because water is often a source of tension between households, household connections improve social integration and reduce conflict. Overall, within 6 months, self-reported well-being improved substantially among households in the treatment group, despite the financial cost of the connection. Our results suggest that facilitating access to credit for households to finance lump sum quality-of-life investments can significantly increase welfare, even if those investments do not result in income or health gains

QuickSNP: an automated web server for selection of tagSNPs

Although large-scale genetic association studies involving hundreds to thousands of SNPs have become feasible, the associated cost is substantial. Even with the increased efficiency introduced by the use of tagSNPs, researchers are often seeking ways to maximize resource utilization given a set of SNP-based gene-mapping goals. We have developed a web server named QuickSNP in order to provide cost-effective selection of SNPs, and to fill in some of the gaps in existing SNP selection tools. One useful feature of QuickSNP is the option to select only gene-centric SNPs from a chromosomal region in an automated fashion. Other useful features include automated selection of coding non-synonymous SNPs, SNP filtering based on inter-SNP distances and information regarding the availability of genotyping assays for SNPs and whether they are present on whole genome chips. The program produces user-friendly summary tables and results, and a link to a UCSC Genome Browser track illustrating the position of the selected tagSNPs in relation to genes and other genomic features. We hope the unique combination of features of this server will be useful for researchers aiming to select markers for their genotyping studies. The server is freely available and can be accessed at the URL http://bioinformoodics.jhmi.edu/quickSNP.pl

Validation and assessment of variant calling pipelines for next-generation sequencing

Background: The processing and analysis of the large scale data generated by next-generation sequencing (NGS) experiments is challenging and is a burgeoning area of new methods development. Several new bioinformatics tools have been developed for calling sequence variants from NGS data. Here, we validate the variant calling of these tools and compare their relative accuracy to determine which data processing pipeline is optimal. Results: We developed a unified pipeline for processing NGS data that encompasses four modules: mapping, filtering, realignment and recalibration, and variant calling. We processed 130 subjects from an ongoing whole exome sequencing study through this pipeline. To evaluate the accuracy of each module, we conducted a series of comparisons between the single nucleotide variant (SNV) calls from the NGS data and either gold-standard Sanger sequencing on a total of 700 variants or array genotyping data on a total of 9,935 single-nucleotide polymorphisms. A head to head comparison showed that Genome Analysis Toolkit (GATK) provided more accurate calls than SAMtools (positive predictive value of 92.55% vs. 80.35%, respectively). Realignment of mapped reads and recalibration of base quality scores before SNV calling proved to be crucial to accurate variant calling. GATK HaplotypeCaller algorithm for variant calling outperformed the UnifiedGenotype algorithm. We also showed a relationship between mapping quality, read depth and allele balance, and SNV call accuracy. However, if best practices are used in data processing, then additional filtering based on these metrics provides little gains and accuracies of >99% are achievable. Conclusions: Our findings will help to determine the best approach for processing NGS data to confidently call variants for downstream analyses. To enable others to implement and replicate our results, all of our codes are freely available at http://metamoodics.org/wes

Exonic DNA Sequencing of ERBB4 in Bipolar Disorder

The Neuregulin-ErbB4 pathway plays a crucial role in brain development and constitutes one of the most biologically plausible signaling pathways implicated in schizophrenia and, to a lesser extent, in bipolar disorder (BP). However, recent genome-wide association analyses have not provided evidence for common variation in NRG1 or ERBB4 influencing schizophrenia or bipolar disorder susceptibility. In this study, we investigate the role of rare coding variants in ERBB4 in BP cases with mood-incongruent psychotic features, a form of BP with arguably the greatest phenotypic overlap with schizophrenia. We performed Sanger sequencing of all 28 exons in ERBB4, as well as part of the promoter and part of the 3′UTR sequence, hypothesizing that rare deleterious variants would be found in 188 cases with mood-incongruent psychosis from the GAIN BP study. We found 42 variants, of which 16 were novel, although none were non-synonymous or clearly deleterious. One of the novel variants, present in 11.2% of cases, is located next to an alternative stop codon, which is associated with a shortened transcript of ERBB4 that is not translated. We genotyped this variant in the GAIN BP case-control samples and found a marginally significant association with mood-incongruent psychotic BP compared with controls (additive model: OR = 1.64, P-value = 0.055; dominant model: OR = 1.73. P-value = 0.039). In conclusion, we found no rare variants of clear deleterious effect, but did uncover a modestly associated novel variant that could affect alternative splicing of ERBB4. However, the modest sample size in this study cannot definitively rule out a role for rare variants in bipolar disorder and studies with larger sample sizes are needed to confirm the observed association

A Hybrid Likelihood Model for Sequence-Based Disease Association Studies

In the past few years, case-control studies of common diseases have shifted their focus from single genes to whole exomes. New sequencing technologies now routinely detect hundreds of thousands of sequence variants in a single study, many of which are rare or even novel. The limitation of classical single-marker association analysis for rare variants has been a challenge in such studies. A new generation of statistical methods for case-control association studies has been developed to meet this challenge. A common approach to association analysis of rare variants is the burden-style collapsing methods to combine rare variant data within individuals across or within genes. Here, we propose a new hybrid likelihood model that combines a burden test with a test of the position distribution of variants. In extensive simulations and on empirical data from the Dallas Heart Study, the new model demonstrates consistently good power, in particular when applied to a gene set (e.g., multiple candidate genes with shared biological function or pathway), when rare variants cluster in key functional regions of a gene, and when protective variants are present. When applied to data from an ongoing sequencing study of bipolar disorder (191 cases, 107 controls), the model identifies seven gene sets with nominal p-values<0.05, of which one MAPK signaling pathway (KEGG) reaches trend-level significance after correcting for multiple testing. © 2013 Chen et al

CSSs in a sample of B2 radio sources of intermediate strength

We present radio observations of 19 candidate compact steep-spectrum (CSS) objects selected from a well-defined, complete sample of 52 B2 radio sources of intermediate strength. These observations were made with the VLA A-array at 4.835 GHz. The radio structures of the entire sample are summarised and the brightness asymmetries within the compact sources are compared with those of the more extended ones, as well as with those in the 3CRR sample and the CSSs from the B3-VLA sample. About 25 per cent of the CSS sources exhibit large brightness asymmetries, with a flux density ratio for the opposing lobes of $>$5, possibly due to interaction of the jets with infalling material. The corresponding percentage for the larger-sized objects is only about 5 per cent. We also investigate possible dependence of the flux density asymmetry of the lobes on redshift, since this might be affected by more interactions and mergers in the past. No such dependence is found. A few individual objects of interest are discussed in the paper.Comment: 10 pages, 7 figures, 2 tables; accepted for publication in Astronomy and Astrophysic