Operational framework based on modeling languages to support product repository implementation.

Part 3: Tools and MethodologiesInternational audienceEmbracing Product Lifecycle Management approach involves integrating a product repository in the company information system. From customer's needs to disposal stage, several product representations exist. The product repository purpose is to secure consistency of one product representation with the others. This paper presents an operational modeling framework that supports product repository implementation. In order to ensure consistency, this framework identifies correspondences between entities of languages (“trade” languages and standard languages). The presented concepts are illustrated with correspondences between language entities of product designed and productplanned to be built Bills of Materials

Targeting the Replication Initiator of the Second Vibrio Chromosome: Towards Generation of Vibrionaceae-Specific Antimicrobial Agents

The Vibrionaceae is comprised of numerous aquatic species and includes several human pathogens, such as Vibrio cholerae, the cause of cholera. All organisms in this family have two chromosomes, and replication of the smaller one depends on rctB, a gene that is restricted to the Vibrionaceae. Given the increasing prevalence of multi-drug resistance in pathogenic vibrios, there is a need for new targets and drugs to combat these pathogens. Here, we carried out a high throughput cell-based screen to find small molecule inhibitors of RctB. We identified a compound that blocked growth of an E. coli strain bearing an rctB-dependent plasmid but did not influence growth of E. coli lacking this plasmid. This compound, designated vibrepin, had potent cidal activity against V. cholerae and inhibited the growth of all vibrio species tested. Vibrepin blocked RctB oriCII unwinding, apparently by promoting formation of large non-functional RctB complexes. Although vibrepin also appears to have targets other than RctB, our findings suggest that RctB is an attractive target for generation of novel antibiotics that only block growth of vibrios. Vibrio-specific agents, unlike antibiotics currently used in clinical practice, will not engender resistance in the normal human flora or in non-vibrio environmental microorganisms

SPI observations of the diffuse 60Fe emission in the Galaxy

Gamma-ray line emission from radioactive decay of 60Fe provides constraints on nucleosynthesis in massive stars and supernovae. The spectrometer SPI on board INTEGRAL has accumulated nearly three years of data on gamma-ray emission from the Galactic plane. We have analyzed these data with suitable instrumental-background models and sky distributions to produce high-resolution spectra of Galactic emission. We detect the gamma-ray lines from 60Fe decay at 1173 and 1333 keV, obtaining an improvement over our earlier measurement of both lines with now 4.9 sigma significance for the combination of the two lines. The average flux per line is (4.4 \pm 0.9) \times 10^{-5} ph cm^{-2} s^{-1} rad^{-1} for the inner Galaxy region. Deriving the Galactic 26Al gamma-ray line flux with using the same set of observations and analysis method, we determine the flux ratio of 60Fe/26Al gamma-rays as 0.148 \pm 0.06. The current theoretical predictions are still consistent with our result.Comment: 10 pages, 7 figures, 2 tables, A&A in pres

Fragmentation and mass segregation in the massive dense cores of Cygnus X

We present Plateau de Bure interferometer observations obtained in continuum at 1.3 and 3.5 mm towards the six most massive and young (IR-quiet) dense cores in Cygnus X. Located at only 1.7 kpc, the Cygnus X region offers the opportunity of reaching small enough scales (of the order of 1700 AU at 1.3 mm) to separate individual collapsing objects. The cores are sub-fragmented with a total of 23 fragments inside 5 cores. Only the most compact core, CygX-N63, could actually be a single massive protostar with an envelope mass as large as 60 Msun. The fragments in the other cores have sizes and separations similar to low-mass pre-stellar and proto-stellar condensations in nearby protoclusters, and are probably of the same nature. A total of 9 out of these 23 protostellar objects are found to be probable precursors of OB stars with envelope masses ranging from 6 to 23 Msun. The level of fragmentation is globally higher than in the turbulence regulated, monolithic collapse scenario, but is not as high as expected in a pure gravo-turbulent scenario where the distribution of mass is dominated by low-mass protostars/stars. Here, the fractions of the total core masses in the high-mass fragments are reaching values as high as 28, 44, and 100 % in CygX-N12, CygX-N53, and CygX-N63, respectively, much higher than what an IMF-like mass distribution would predict. The increase of the fragmentation efficiency as a function of density in the cores is proposed to be due to the increasing importance of self-gravity leading to gravitational collapse at the scale of the dense cores. At the same time, the cores tend to fragment into a few massive protostars within their central regions. We are therefore probably witnessing here the primordial mass segregation of clusters in formation.Comment: 14 pages, 16 figures, submitted for publication in A&

The Scientific Performance of the Microchannel X-ray Telescope on board the SVOM Mission

The Microchannel X-ray Telescope (MXT) will be the first focusing X-ray telescope based on a "Lobster-Eye" optical design to be flown on Sino-French mission SVOM. SVOM will be dedicated to the study of Gamma-Ray Bursts and more generally time-domain astrophysics. The MXT telescope is a compact (focal length ~ 1.15 m) and light (< 42 kg) instrument, sensitive in the 0.2--10 keV energy range. It is composed of an optical system, based on micro-pore optics (MPOs) of 40 micron pore size, coupled to a low-noise pnCDD X-ray detector. In this paper we describe the expected scientific performance of the MXT telescope, based on the End-to-End calibration campaign performed in fall 2021, before the integration of the SVOM payload on the satellite.Comment: 22 pages, 12 figures, accepted for publication in Experimental Astronom

PLM in SME, what are we missing? an alternative view on PLM implementation for SME

Part 10: PLM Maturity, Implementation and AdoptionInternational audienceToday, the concept of Product Lifecycle Management (PLM) is widely accepted as strategically important. It is used to manage the increasing complexity of products, processes and organizations. The need to adopt PLM is growing rapidly for Small to Medium-sized Enterprises (SME). PLM implementations are costly and require a lot of effort. The business impact and financial risks are high for SME. Also, SMEs seem to have relatively more difficulties to benefit from PLM. The study at hand addresses the question, based on literature research, why these difficulties exist and how they can be overcome. To answer that question, three sub questions are discussed in this paper. (1) A generic PLM implementation process structure. (2) A list of identified PLM implementation challenges, specific for SME. (3) A classification of PLM research for SME, related to the common PLM implementation process structure. A hypothesis for a PLM implementation failure mechanism in SMEs is formulated, based on the findings. Also, a potential research gap on operational implementation knowledge in SMEs is identified

Regulatory Cross-Talk Links Vibrio cholerae Chromosome II Replication and Segregation

There is little knowledge of factors and mechanisms for coordinating bacterial chromosome replication and segregation. Previous studies have revealed that genes (and their products) that surround the origin of replication (oriCII) of Vibrio cholerae chromosome II (chrII) are critical for controlling the replication and segregation of this chromosome. rctB, which flanks one side of oriCII, encodes a protein that initiates chrII replication; rctA, which flanks the other side of oriCII, inhibits rctB activity. The chrII parAB2 operon, which is essential for chrII partitioning, is located immediately downstream of rctA. Here, we explored how rctA exerts negative control over chrII replication. Our observations suggest that RctB has at least two DNA binding domains—one for binding to oriCII and initiating replication and the other for binding to rctA and thereby inhibiting RctB's ability to initiate replication. Notably, the inhibitory effect of rctA could be alleviated by binding of ParB2 to a centromere-like parS site within rctA. Furthermore, by binding to rctA, ParB2 and RctB inversely regulate expression of the parAB2 genes. Together, our findings suggest that fluctuations in binding of the partitioning protein ParB2 and the chrII initiator RctB to rctA underlie a regulatory network controlling both oriCII firing and the production of the essential chrII partitioning proteins. Thus, by binding both RctB and ParB2, rctA serves as a nexus for regulatory cross-talk coordinating chrII replication and segregation

FtsK-Dependent Dimer Resolution on Multiple Chromosomes in the Pathogen Vibrio cholerae

Unlike most bacteria, Vibrio cholerae harbors two distinct, nonhomologous circular chromosomes (chromosome I and II). Many features of chromosome II are plasmid-like, which raised questions concerning its chromosomal nature. Plasmid replication and segregation are generally not coordinated with the bacterial cell cycle, further calling into question the mechanisms ensuring the synchronous management of chromosome I and II. Maintenance of circular replicons requires the resolution of dimers created by homologous recombination events. In Escherichia coli, chromosome dimers are resolved by the addition of a crossover at a specific site, dif, by two tyrosine recombinases, XerC and XerD. The process is coordinated with cell division through the activity of a DNA translocase, FtsK. Many E. coli plasmids also use XerCD for dimer resolution. However, the process is FtsK-independent. The two chromosomes of the V. cholerae N16961 strain carry divergent dimer resolution sites, dif1 and dif2. Here, we show that V. cholerae FtsK controls the addition of a crossover at dif1 and dif2 by a common pair of Xer recombinases. In addition, we show that specific DNA motifs dictate its orientation of translocation, the distribution of these motifs on chromosome I and chromosome II supporting the idea that FtsK translocation serves to bring together the resolution sites carried by a dimer at the time of cell division. Taken together, these results suggest that the same FtsK-dependent mechanism coordinates dimer resolution with cell division for each of the two V. cholerae chromosomes. Chromosome II dimer resolution thus stands as a bona fide chromosomal process