NIRS potential use for the determination of natural resources quality from dehesa (acorn and grass) in Montanera system for Iberian pigs.

NIRS technology has been used as an alternative to conventional methods to determinate the content of nutrients of acorns and grass from dehesa ecosystem. Dry matter (DM), crude fat (CF), crude protein (CP), starch, total phenolic compounds (TP), α-tocopherol, γ-tocopherol, fatty acids, neutral detergent fiber (NDF), total antioxidant activity (TAA) and total energy (TE) were determined by conventional methods for later development of NIRS predictive equations. The NIR spectrum of each sample was collected and for all studied parameters, a predictive model was obtained and external validated. Good prediction equations were obtained for moisture, crude fat, crude protein, total energy and γ-tocopherol in acorns samples, with high coefficients of correlation (1-VR) and low standard error of prediction (SEP) (1-VR=0.81, SEP=2.62; 1-VR=0.92, SEP=0.54; 1-VR=0.86, SEP=0.47; 1-VR=0.84, SEP=0.2; 1-VR=0.88, SEP=5.4, respectively) and crude protein, NDF, α-tocopherol and linolenic acid content in grass samples (1-VR=0.9, SEP=1.99; 1-VR=0.87, SEP=4.13; 1-VR=0.76, SEP=10.9; 1-VR=0.82, SEP=0.6, respectively). Therefore, these prediction models could be used to determinate the nutritional composition of Montanera natural resources

AMP N1-oxide, a unique compound of royal jelly, induces neurite outgrowth from PC12 cells via signaling by protein kinase A independent of that by mitogen-activated protein kinase

Earlier we identified adenosine monophosphate (AMP) N1-oxide as a unique compound of royal jelly (RJ) that induces neurite outgrowth (neuritegenesis) from cultured rat pheochromocytoma PC12 cells via the adenosine A2A receptor. Now, we found that AMP N1-oxide stimulated the phosphorylation of not only mitogen-activated protein kinase (MAPK) but also that of cAMP/calcium-response element-binding protein (CREB) in a dose-dependent manner. Inhibition of MAPK activation by a MEK inhibitor, PD98059, did not influence the AMP N1-oxide-induced neuritegenesis, whereas that of protein kinase A (PKA) by a selective inhibitor, KT5720, significantly reduced neurite outgrowth. AMP N1-oxide also had the activity of suppressing the growth of PC12 cells, which correlated well with the neurite outgrowth-promoting activity. KT5720 restored the growth of AMP N1-oxide-treated PC12 cells. It is well known that nerve growth factor suppresses proliferation of PC12 cells before causing stimulation of neuronal differentiation. Thus, AMP N1-oxide elicited neuronal differentiation of PC12 cells, as evidenced by generation of neurites, and inhibited cell growth through adenosine A2A receptor-mediated PKA signaling, which may be responsible for characteristic actions of RJ

Patient knowledge of fecal calprotectin in inflammatory bowel disease (IBD) : an observational study in Mexico

Background: Fecal calprotectin (FC) can be a valuable tool to optimize health care for patients with inflammatory bowel disease (IBD). The objective of this observational study was to determine the level of knowledge of the FC test in Mexican patients with IBD. Methods: A self-report questionnaire was distributed via Facebook to patients with IBD. The survey consisted of 15 questions in two categories: the first category assessed knowledge of IBD diagnosis, and the second category assessed knowledge of the FC test. Results: In total, 460 patients with IBD participated, of which 83.9% (386) had ulcerative colitis (UC) and 16.0% (74) had Crohn’s disease (CD). Regarding IBD diagnosis, 41.9% of participants stated that they did not know of a non-invasive test for fecal matter to identify inflammation of the colon. Regarding the FC test, 57.5% (UC) and 58.1% (CD) stated that they did not know about the test. Additionally, 65.8% (UC) and 51.3% (CD) of participants stated that they had never received the FC test and 82.6% (UC) and 77.0% (CD) recognized that the FC test was difficult to access in their medical practice. Furthermore, 66% (UC) and 52.7% (CD) of participants noted that their specialist doctor had never suggested the FC test to them, yet 89.1% (UC) and 87.8% (CD) stated that they would prefer FC analysis for their IBD follow-up assessments. Conclusions: There is little knowledge of the FC biomarker among Mexican patients with IBD. This suggests the need for greater dissemination of its use and scope as a biomarker in IBD

Optogenetic Mimicry of the Transient Activation of Dopamine Neurons by Natural Reward Is Sufficient for Operant Reinforcement

Activation of dopamine receptors in forebrain regions, for minutes or longer, is known to be sufficient for positive reinforcement of stimuli and actions. However, the firing rate of dopamine neurons is increased for only about 200 milliseconds following natural reward events that are better than expected, a response which has been described as a “reward prediction error” (RPE). Although RPE drives reinforcement learning (RL) in computational models, it has not been possible to directly test whether the transient dopamine signal actually drives RL. Here we have performed optical stimulation of genetically targeted ventral tegmental area (VTA) dopamine neurons expressing Channelrhodopsin-2 (ChR2) in mice. We mimicked the transient activation of dopamine neurons that occurs in response to natural reward by applying a light pulse of 200 ms in VTA. When a single light pulse followed each self-initiated nose poke, it was sufficient in itself to cause operant reinforcement. Furthermore, when optical stimulation was delivered in separate sessions according to a predetermined pattern, it increased locomotion and contralateral rotations, behaviors that are known to result from activation of dopamine neurons. All three of the optically induced operant and locomotor behaviors were tightly correlated with the number of VTA dopamine neurons that expressed ChR2, providing additional evidence that the behavioral responses were caused by activation of dopamine neurons. These results provide strong evidence that the transient activation of dopamine neurons provides a functional reward signal that drives learning, in support of RL theories of dopamine function

Effect of ketogenic mediterranean diet with phytoextracts and low carbohydrates/high-protein meals on weight, cardiovascular risk factors, body composition and diet compliance in Italian council employees

<p>Abstract</p> <p>Background</p> <p>There has been increased interest in recent years in very low carbohydrate ketogenic diets (VLCKD) that, even though they are much discussed and often opposed, have undoubtedly been shown to be effective, at least in the short to medium term, as a tool to tackle obesity, hyperlipidemia and some cardiovascular risk factors. For this reason the ketogenic diet represents an interesting option but unfortunately suffers from a low compliance. The aim of this pilot study is to ascertain the safety and effects of a modified ketogenic diet that utilizes ingredients which are low in carbohydrates but are formulated to simulate its aspect and taste and also contain phytoextracts to add beneficial effects of important vegetable components.</p> <p>Methods</p> <p>The study group consisted of 106 Rome council employees with a body mass index of ≥ 25, age between 18 and 65 years (19 male and 87 female; mean age 48.49 ± 10.3). We investigated the effects of a modified ketogenic diet based on green vegetables, olive oil, fish and meat plus dishes composed of high quality protein and virtually zero carbohydrate but which mimic their taste, with the addition of some herbal extracts (KEMEPHY ketogenic Mediterranean with phytoextracts). Calories in the diet were unlimited. Measurements were taken before and after 6 weeks of diet.</p> <p>Results</p> <p>There were no significant changes in BUN, ALT, AST, GGT and blood creatinine. We detected a significant (p < 0.0001) reduction in BMI (31.45 Kg/m²to 29.01 Kg/m²), body weight (86.15 kg to 79.43 Kg), percentage of fat mass (41.24% to 34.99%), waist circumference (106.56 cm to 97.10 cm), total cholesterol (204 mg/dl to 181 mg/dl), LDLc (150 mg/dl to 136 mg/dl), triglycerides (119 mg/dl to 93 mg/dl) and blood glucose (96 mg/dl to 91 mg/dl). There was a significant (p < 0.0001) increase in HDLc (46 mg/dl to 52 mg/dl).</p> <p>Conclusions</p> <p>The KEMEPHY diet lead to weight reduction, improvements in cardiovascular risk markers, reduction in waist circumference and showed good compliance.</p

Arqueologia e história indígena no Pantanal

O artigo apresenta uma síntese dos dados arqueológicos sobre o Pantanal e o seu entorno, principalmente em Mato Grosso e Mato Grosso do Sul. Elaborado com base na noção de arqueologia como história indígena de longa duração, o artigo considera as trajetórias de estabelecimento e consolidação territorial da ocupação indígena regional, os processos de formação da configuração etnográfica encontrada pelos europeus e os impactos do colonialismo. O principal objetivo consiste em mostrar que a diversidade cultural característica do cenário etnográfico pantaneiro está associada à dinâmica histórica e cultural da ocupação indígena desde períodos anteriores à chegada dos conquistadores e colonizadores de origem europeia.The article presents an overview of the archaeological data on the Pantanal and its surrounding areas, mainly in Mato Grosso and Mato Grosso do Sul. Prepared based on the notion of archeology as long term indigenous history, the article considers the trajectories of territorial establishment and consolidation of the regional indigenous occupation, the formation processes of ethnographic setting found by Europeans and the impact of colonialism. The main objective is to show that cultural diversity characteristic of the Pantanal ethnographic scenario is associated with historical and cultural dynamics of indigenous occupation from periods prior to the arrival of the conquistadors and settlers of European origin

Variabilidad interanual y evolución durante el periodo de montanera de bellotas de Quercus rotundifolia Lam

Eighty-eight samples of Quercus rotundifolia acorns were analyzed during three consecutive years (2005/06, 2006/07 and 2007/08) to determine variations in their morphology, sanitary condition, and nutritional composition throughout the same period of montanera (free-range swine-raising system of SW Iberia) and between different years of study. Results showed that morphological characteristics remained constant throughout montanera season. Fungal damage increased, and Curculio sp. and Cydia sp. pests decreased over the course of the montanera season. Dry matter content decreased in both kernel and pericarp (P < 0.001), while the crude fiber and protein contents were not modified. The ash, nitrogen-free extract, and fat contents, however, were also affected by sampling date within a montanera season. With respect to the interannual variability, variations (P < 0.05) were observed in all the variables studied. It is therefore confirmed that there is variability in the sanitary condition and nutritional composition of acorns within the same montanera season and between different years of study. These could explain the differences in the chemical characteristics of Iberian pig tissues from animals raised in different montanera seasons, and even during the same montanera season.Ochenta y ocho muestras de bellotas de Quercus rotundifolia fueron analizadas durante tres años consecutivos (2005/06, 2006/07 y 2007/08), para determinar variaciones en la morfología, estado sanitario y composición nutritiva de las mismas durante un mismo periodo de montanera y entre diferentes años de estudio. Los resultados mostraron que las características morfológicas permanecen constantes durante la montanera. El ataque debido a la presencia de hongos aumentó, mientras que el de los insectos Curculio sp. y Cydia sp. disminuyó durante la montanera. El contenido en materia seca, tanto de la semilla como del pericarpio, también disminuyó (P < 0,001), mientras que el contenido en fibra bruta y en proteína no sufrió modificaciones. Sin embargo, el contenido en cenizas, sustancias extractivas libres de nitrógeno y grasa también se vio afectado por la fecha de muestreo dentro de una montanera. Con respecto a la variabilidad interanual, se observaron variaciones (P < 0,05) en todas las variables estudiadas. Por tanto, se puede afirmar que existe variabilidad en el estado sanitario y la composición nutritiva de bellotas dentro de una misma montanera y entre diferentes años de estudio. Esto podría explicar las variaciones en las características químicas de los tejidos de cerdo ibéricos de animales criados en diferentes periodos de montanera, e incluso en una misma campaña

Narcissus sect. Jonquillae DC. (Amaryllidaceae) en Extremadura (España)

The revision of the section: Narcissus sect. Jonquillae DC., from Extremadura has revealed the next morphological connection between different taxa, is clarified the morphological range of the others known species such as N. jonquilla L. and N. fernandesii Pedro, and were typified the taxa: N. fernandesii Pedro, N. jonquilla var. heriquesii Samp., N. fernandesii var. major A.Fern., N. jonquilloides var. willkommii Samp. and N. xintermedius Loisel. Also were studied the distribution, ecology for all the species. Finally are present the following new chorological taxa from Extremadura territories: N. assoanus subsp. praelongus Barra & G.López, N. gaditanus Boiss. & Reuter, N. blanchardii Zonn. and N. willkommii (Samp.) A.Fern.morfológica de algunas especies, se aclara el rango de variación de otras conocidas como N. jonquilla L. o N. fernandesii Pedro, y se tipifican los taxones N. fernandesii Pedro, N. jonquilla var. heriquesii Samp., N. fernandesii var. major A.Fern., N. jonquilloides var. willkommii Samp. y N. xintermedius Loisel. Se estudia la distribución y ecología de todas las especies presentes en Extremadura. Además se presenta como novedades corológicas para el territorio extremeño los siguientes taxones: N. assoanus subsp. praelongus Barra & G.López, N. gaditanus Boiss. & Reuter, N. blanchardii Zonn. y N. willkommii (Samp.) A.Fern