Physicochemical Characteristics of Saudi Arabian Locally Produced Raw and Diluted Honeys and Their Relations to Antimicrobial Activity

The physicochemical characteristics and antibacterial activity of Saudi Arabia honeys were studied for the first time. The levels of free and total acidity, pH, ash and moisture content were in the range 1.6 ±0.17 - 15.1 ±0.1 meq/kg, 2.77 ± 0.06 - 5.37 ±0.04, 1.1 ±0.02 – 1.7 ±0.03 % and < 18.0 %, respectively. Lovibond comparator color scale (P, mm) of samples was ranged from water white (P=0.0-1.3), extra light Amber (P=38.14-46.57), light Amber (P=60.39-75.54), Amber (P=86.72-110.08), dark (P=142.39-348.44) and very dark shade (P= 541.84).  Dark honeys showed excellent inhibitory effects against bacterial growth. Excellent correlation between color of raw and diluted (>10.0%m/v) honey and antimicrobial activity was noticed. Honey species from different floral sources posses’ strong antioxidant and anti bacterial activities and are scavengers of active oxygen species.Â

A Study of Epiphytic and Epipelic Algae in Al-Dora Site/Tigris River in Bagdad Province- Iraq

There is a scarcity of data regarding algal flora of Tigris River in the territory of Baghdad. The present study deals with Tigris River in Al-Dora site in Baghdad province from November 2014 to June 2015 in order to shed light on its epiphytic Algae on (Phragmites australis) and epipelic algae. An amount of 183 and 154 species of epiphytic and epipelic algae are identified respectfully. The Bacillariophyceae (diatoms) are the dominant algal group followed by Cyanophyceae and Chlorophyceae. Moreover, 90 species are shared between two groups of algae (epiphytic and epipelic) and identified at the study site. Additionally, the seasonal variations and diversity of algal species are noticed. The highest number of epiphytic algae is 772.05 x 104 (cell/gm) in winter and the lowest number is 161.13 x 104 (cell/gm) in Summer; where as the highest number of epipelic algae is 20.07 x 104 (cell/ cm²) in Winter and the lowest number is 6.53 x 104 (cell/ cm²) in Summer

A compact UWB monopole antenna with penta band notched characteristics

A modified rectangular monopole ultra-wideband (UWB) antenna with penta notched frequency bands is presented. An inverted U shaped and slanted U-shaped on the radiating patch are inserted to achieve WiMAX and ARN bands rejection respectively, two mirrored summation Σ-shaped and four mirrored 5-shaped slots are inserted on the partial ground to achieve WLAN and X-band bands rejection respectively, finally rectangular shaped slot with partially open on the feed is inserted to achieve ITU-8 band rejection. The proposed antenna which was simulated has a compact size 30×35×1.6 m3. It is operated with impedance bandwidth 2.8-10.6 GHz at |S11| < −10 dB, that supported UWB bandwidth with filtering the five narrowbands that avoid the possible interference with them. The simulated resonant frequency for notched filters received 3.55, 4.55, 5.53, 7.45, 8.16 GHZ, for WiMAX, ARN, WLAN, X-Band, ITU-8 respectively. The proposed antenna is suitable for wireless communication such as mobile communication and internet of everything (IoE). Throughout this paper, CST-EM software package was used for the design implementation. Surface current distributions for all notched filters were investigated and shown that it is concentrated around the feeding point and the inserted notched slots proving that there is no radiation to the space due to maximum stored electromagnetic energy around each investigated notch slot, proving that the slots play a role of a quarter wavelength transformer which generates for each notched band, maximum gain, and radiation pattern are also investigated

Filistin Gazze’de Al-Azhar Üniversitesi öğrencilerinin HIV/AIDS’e yönelik bilgi ve tutumlarının değerlendirilmesi

Objective: The study assessed the knowledge and attitudes of students’ at a university in Gaza regarding HIV/AIDS and identified differences in knowledge about HIV, and attitudes by gender, locality, and social and economic status. Methods: This descriptive study targeted 492 students of Al-Azhar University-Gaza. The participants completed self-administered questionnaires that included the following dimensions: socio-demographic, measurements of student's knowledge level and measurement of student's attitudes towards HIV/AIDS. Results: Findings showed moderate level of knowledge regarding HIV/AIDS although there was a very low of perception regarding the acceptance and sharing of HIV/AIDS persons. It means that only one third of the study respondents are willingness to be in close touch with people living with HIV or even communication with them, and less than fifty percent thought that it is their right to be engaged in a public or governmental job, stigma and discriminatory attitudes toward HIV/AIDS persons is high only 48% of the students thought it right to employ people living with HIV (PLHIV) and 35.5% refused to work in the same place with PLHIV. Conclusion: This study indicates the need for improving the level of knowledge as well as promoting the students' towards positive attitude

Weight Reduction Perception and Practice among Female Students in Al-Azhar University-Gaza, Palestine

Objectives: The present study aimed to determine the frequency of weight reduction perception. Methods: A sample of 467 female students from Al-Azhar University in the Gaza Strip was selected on a convenience basis. Information obtained from the participants included: age, marital status, place of residence, employment status, health status, methods of weight reduction, sources of information about weight control, and current physical activities. Results: The present study found that 38.8% of female students had practiced weight reduction. The most prevailing attitudes for reducing weight described by the students was sports with 83.3%, followed by exclusion of some principal meals or principal nutrients (57.8%). It was found that 38.5% of the students did not practice any physical exercise. An inverse association was also present between the satisfaction about body weight and the practice of weight reduction

The “Big Six”: Hidden Emerging Foodborne Bacterial Pathogens

Non-O157 Shiga toxin-producing Escherichia coli (STEC) are emerging serogroups that often result in diseases ranging from diarrhea to severe hemorrhagic colitis in humans. The most common non-O157 STEC are O26, O45, O103, O111, O121, and O145. These serogroups are known by the name “big six” because they cause severe illness and death in humans and the United States Department of Agriculture declared these serogroups as food contaminants. The lack of fast and efficient diagnostic methods exacerbates the public impact of the disease caused by these serogroups. Numerous outbreaks have been reported globally and most of these outbreaks were caused by ingestion of contaminated food or water as well as direct contact with reservoirs. Livestock harbor a variety of non-O157 STEC serovars that can contaminate meat and dairy products, or water sources when used for irrigation. Hence, effective control and prevention approaches are required to safeguard the public from infections. This review addresses the disease characteristics, reservoirs, the source of infections, the transmission of the disease, and major outbreaks associated with the six serogroups (“big six”) of non-O157 STEC encountered all over the glob

Physiological Biomimetic Culture System for Pig and Human Heart Slices

RATIONALE: Preclinical testing of cardiotoxicity and efficacy of novel heart failure therapies faces a major limitation: the lack of an in situ culture system that emulates the complexity of human heart tissue and maintains viability and functionality for a prolonged time. OBJECTIVE: To develop a reliable, easily reproducible, medium-throughput method to culture pig and human heart slices under physiological conditions for a prolonged period of time. METHODS AND RESULTS: Here, we describe a novel, medium-throughput biomimetic culture system that maintains viability and functionality of human and pig heart slices (300 µm thickness) for 6 days in culture. We optimized the medium and culture conditions with continuous electrical stimulation at 1.2 Hz and oxygenation of the medium. Functional viability of these slices over 6 days was confirmed by assessing their calcium homeostasis, twitch force generation, and response to β-adrenergic stimulation. Temporal transcriptome analysis using RNAseq at day 2, 6, and 10 in culture confirmed overall maintenance of normal gene expression for up to 6 days, while over 500 transcripts were differentially regulated after 10 days. Electron microscopy demonstrated intact mitochondria and Z-disc ultra-structures after 6 days in culture under our optimized conditions. This biomimetic culture system was successful in keeping human heart slices completely viable and functionally and structurally intact for 6 days in culture. We also used this system to demonstrate the effects of a novel gene therapy approach in human heart slices. Furthermore, this culture system enabled the assessment of contraction and relaxation kinetics on isolated single myofibrils from heart slices after culture. CONCLUSIONS: We have developed and optimized a reliable medium-throughput culture system for pig and human heart slices as a platform for testing the efficacy of novel heart failure therapeutics and reliable testing of cardiotoxicity in a 3D heart model

Towards transnational feminist queer methodologies

This article introduces the possibilities of transnational feminist queer research as seeking to conceptualise the transnational as a methodology composed of a series of flows that can augment feminist and queer research. Transnational feminist queer methodologies can contest long-standing configurations of power between researcher and researched, subject and object, academics and activists across places, typically those which are embedded in the hierarchies of the Global North/Global South. Beginning with charting our roots in, and routes through, the diverse arenas of transnational, feminist, participatory and queer methodologies, the article uses a transcribed and edited conversation between members of the Liveable Lives research team in Kolkata and Brighton, to start an exploration of transnational feminist queer methodologies. Understanding the difficult, yet constructive moments of collaborative work and dialogue, we argue for engagements with the multiplicities of ‘many-many' lives that recognise local specificities, and the complexities of lives within transnational research, avoiding creating a currency of comparison between places. We seek to work toward methodologies that take seriously the politics of place, namely by creating research that answers the same question in different places, using methods that are created in context and may not be ‘comparable'. Using a dialogue across the boundaries of activism/academia, as well as across geographical locations, the article contends that there are potentials, as well as challenges, in thinking ourselves through transnational research praxis. This seeks complexities and spatial nuances within as well as between places

Bone sarcomas: ESMO-EURACAN-GENTURIS-ERN PaedCan Clinical Practice Guideline for diagnosis, treatment and follow-up

This Clinical Practice Guideline provides key recommendations on the management of bone sarcomas. // Recommendations have been agreed following a consensus meeting of representatives from ESMO, EURACAN, GENTURIS and ERNPaedCan. // Authorship includes a multidisciplinary group of experts from different institutions and countries worldwide

P2RX7 Purinoceptor: A Therapeutic Target for Ameliorating the Symptoms of Duchenne Muscular Dystrophy

open access articleDuchenne muscular dystrophy (DMD) is the most common inherited muscle disease, leading to severe disability and death in young men. Death is caused by the progressive degeneration of striated muscles aggravated by sterile inflammation. The pleiotropic effects of the mutant gene also include cognitive and behavioral impairments and low bone density. Current interventions in DMD are palliative only as no treatment improves the long-term outcome. Therefore, approaches with a translational potential should be investigated, and key abnormalities downstream from the absence of the DMD product, dystrophin, appear to be strong therapeutic targets. We and others have demonstrated that DMD mutations alter ATP signaling and have identified P2RX7 purinoceptor up-regulation as being responsible for the death of muscles in the mdx mouse model of DMD and human DMD lymphoblasts. Moreover, the ATP–P2RX7 axis, being a crucial activator of innate immune responses, can contribute to DMD pathology by stimulating chronic inflammation. We investigated whether ablation of P2RX7 attenuates the DMD model mouse phenotype to assess receptor suitability as a therapeutic target