Nanocarriers used as probes for super-resolution microscopy

Super-resolution microscopy (SRM) has revolutionized the study of cell biology, enabling researchers to visualize cellular structures with nanometric resolution, single-molecule sensitivity, and with multiple colors using conventional fluorophores. SRM is well-suited for volumetric live-cell imaging and helps in extracting quantitative information on spatial distributions. It can be used to estimate the absolute numbers of proteins or other macromolecules or nanostructured material within subcellular compartments, characterize their structures, and their nano/bio interactions. Although a number of recent general reviews on SRM have elaborated its role in chemical and clinical biology, as well as nanomedicine, herein, we provide an overview of the use of luminescent nanocarriers (LNC) in SRM imaging and single-molecule tracking. The role of LNCs in controlling the brightness and stability of emissive states is discussed with a special focus on organelle-specific delivery and how this approach can be utilized to produce novel optical-switched systems. We also discuss the challenges related to the molecular targeting of such material in biological systems. In doing so, we will provide practical guidance for super-resolution imaging in nanomedicine research, its technical challenges, and the opportunities for future advancement

Nanocarriers used as probes for super-resolution microscopy

Super-resolution microscopy (SRM) has revolutionized the study of cell biology, enabling researchers to visualize cellular structures with nanometric resolution, single-molecule sensitivity, and with multiple colors using conventional fluorophores. SRM is well-suited for volumetric live-cell imaging and helps in extracting quantitative information on spatial distributions. It can be used to estimate the absolute numbers of proteins or other macromolecules or nanostructured material within subcellular compartments, characterize their structures, and their nano/bio interactions. Although a number of recent general reviews on SRM have elaborated its role in chemical and clinical biology, as well as nanomedicine, herein, we provide an overview of the use of luminescent nanocarriers (LNC) in SRM imaging and single-molecule tracking. The role of LNCs in controlling the brightness and stability of emissive states is discussed with a special focus on organelle-specific delivery and how this approach can be utilized to produce novel optical-switched systems. We also discuss the challenges related to the molecular targeting of such material in biological systems. In doing so, we will provide practical guidance for super-resolution imaging in nanomedicine research, its technical challenges, and the opportunities for future advancement

Outcomes of polytrauma patients with diabetes mellitus.

BACKGROUND: The impact of diabetes mellitus in patients with multiple system injuries remains obscure. This study was designed to increase knowledge of outcomes of polytrauma in patients who have diabetes mellitus. METHODS: Data from the Trauma Audit and Research Network was used to identify patients who had suffered polytrauma during 2003 to 2011. These patients were filtered to those with known outcomes, then separated into those with diabetes, those known to have other co-morbidities but not diabetes and those known not to have any co-morbidities or diabetes. The data were analyzed to establish if patients with diabetes had differing outcomes associated with their diabetes versus the other groups. RESULTS: In total, 222 patients had diabetes, 2,558 had no past medical co-morbidities (PMC), 2,709 had PMC but no diabetes. The diabetic group of patients was found to be older than the other groups (P <0.05). A higher mortality rate was found in the diabetic group compared to the non-PMC group (32.4% versus 12.9%), P <0.05). Rates of many complications including renal failure, myocardial infarction, acute respiratory distress syndrome, pulmonary embolism and deep vein thrombosis were all found to be higher in the diabetic group. CONCLUSIONS: Close monitoring of diabetic patients may result in improved outcomes. Tighter glycemic control and earlier intervention for complications may reduce mortality and morbidity

Isolated patellofemoral osteoarthritis: A systematic review of treatment options using the GRADE approach

Background and purpose The optimal treatment for isolated patellofemoral osteoarthritis is unclear at present. We systematically reviewed the highest level of available evidence on the nonoperative and operative treatment of isolated patellofemoral osteoarthritis to develop an evidenced-based discussion of treatment options

A diverse view of science to catalyse change:Valuing diversity leads to scientific excellence, the progress of science and, most importantly, it is simply the right thing to do. we must value diversity not only in words, but also in actions

No abstract available.publishe

A diverse view of science to catalyse change

Valuing diversity leads to scientific excellence, the progress of science and, most importantly, it is simply the right thing to do. We must value diversity not only in words, but also in actions

THE SOCIAL IMPLICATIONS OF SOME NIGERIAN YORUBA AND IBO PROVERBS ON CAREFULNESS

ABSTRACT : The purpose of this Article is to show how th