How do early socioeconomic circumstances impact inflammatory trajectories? Findings from Generation XXI

© 2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/. This version of the article [S. Soares, A. López-Cheda, A. C. Santos, H. Barros, y S. Fraga, «How do early socioeconomic circumstances impact inflammatory trajectories? Findings from Generation XXI», Psychoneuroendocrinology, vol. 119, p. 104755, sep. 2020] has been accepted for publication in Psychoneuroendocrinology. The Version of Record is available online at https://doi.org/10.1016/j.psyneuen.2020.104755.[Abstract]: Background: The association between socioeconomic position and markers of inflammation in adults, including C-reactive protein (CRP), is well-established. We hypothesized that children from families of less-advantaged socioeconomic circumstances may be at higher inflammatory risk during childhood and, consequently, throughout their life course. Thus, we aimed to investigate whether early socioeconomic circumstances impact CRP trajectories using repeated measures of data from a population-based birth cohort. Methods: Data from 2510 participants of Generation XXI, a prospective Portuguese population-based birth cohort, were included in this study. Early socioeconomic circumstances comprised maternal education and occupation, paternal education and occupation, and household income at the child’s birth. Venous blood samples were collected from the children at ages four, seven, and ten years, and high-sensitivity CRP (Hs-CRP) was quantified. Hs-CRP trajectories were computed using a linear mixed-model approach. Results: Participants from less-advantaged socioeconomic circumstances presented higher levels of Hs-CRP by age of ten years. The higher the mother´s education and disposable household income, the lower the minimum value of the log Hs-CRP observed throughout childhood. Further, the age at which that minimum log Hs-CRP value was reached occurs later, meaning that children born in more-advantaged socioeconomic circumstances had lower levels of log Hs-CRP compared with children from less-advantaged families. Conclusions: Poor socioeconomic circumstances early in life are associated with increased inflammation levels throughout the first decade of life. This study demonstrates that social inequalities may impact population health beginning at very early ages.This work was supported by the European Regional Development Fund through the Operational Programme Competitiveness and Internationalization and national funding from the Foundation for Science and Technology (FCT), Portuguese Ministry of Science, Technology, and Higher Education under the projects “BioAdversity: How childhood social adversity shapes health: The biology of social adversity” (POCI-01- 0145-FEDER-016838; reference FCT PTDC/DTP-EPI/1687/2014), “HIneC: When do health inequalities start? Understanding the impact of childhood social adversity on health trajectories from birth to early adolescence” (POCI-01-0145-FEDER-029567; reference: FCT PTDC/SAU-PUB/29,567/2017). It is also supported by the Unidade de Investigação em Epidemiologia–Instituto de Saúde Pública da Universidade do Porto (EPIUnit) (reference UIDB/04750/2020), Administração Regional de Saúde Norte (Regional Department of Ministry of Health) and Fundação Calouste Gulbenkian; PhD grant SFRH/BD/108742/2015 (to SS) co-funded by FCT and the Human Capital Operational Programme (POCH/FSE Program); FCT Investigator contracts CEECIND/01516/2017 (to SF) and IF/01060/2015 (to ACS); and BEATRIZ GALINDO JUNIOR Spanish Grant (code BEAGAL18/00143) from MICINN (Ministerio de Ciencia, Innovación y Universidades), reference BGP18/00154 (to ALC). This study is also a result of the project DOCnet (NORTE-01-0145-FEDER-000003), supported by the Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement.Portugal. Ministério da Ciência, Tecnologia e Ensino Superior; FCT PTDC/DTP-EPI/1687/2014Portugal. Ministério da Ciência, Tecnologia e Ensino Superior; FCT PTDC/SAU-PUB/29,567/2017Universidade do Porto; UIDB/04750/202

GESTÃO DO CONHECIMENTO EM UMA INSTITUIÇÃO PÚBLICA DE PESQUISA: UM ESTUDO NO CENTRO DE INFORMAÇÕES TECNOLÓGICAS E COMERCIAIS PARA FRUTICULTURA TROPICAL

For many companies, the first attempt of manage organizational knowledge has been the construction of information stores. However, only the technology infrastructure does not guarantee the success of an initiative of knowledge management. In this context, this study has the purpose to identify the factors that had influenced in the process of implantation of the Center for Technological Information and Trade of Tropical Fruit (Centro de Informações Tecnológicas e Comerciais para Fruticultura Tropical – CEINFO), an initiative of knowledge management of the Embrapa Agroindústria Tropical. This quanti-qualitative research is a case study with methodology of participant research. The results showed that the company gave emphasis in the technology for the solution of problems of knowledge management without consider human and cultures issues, what characterize a mechanical approach. Some factors influenced on knowledge management such as low level of participation and corporate communication; reactive culture of the researched group, harming the information sharing and the socialization of knowledge.A construção de repositórios de informações tem sido para muitas empresas, a primeira tentativa de gerir o conhecimento organizacional. Contudo, apenas a infra-estrutura tecnológica não garante o sucesso de uma iniciativa de gestão do conhecimento. Nesse contexto, busca-se, com o presente estudo, identificar os fatores que influenciaram o processo de implantação do Centro de Informações Tecnológicas e Comerciais para Fruticultura Tropical (CEINFO), uma iniciativa de gestão do conhecimento da Embrapa Agroindústria Tropical. A pesquisa de natureza quanti-qualitativa fez uso do estudo de caso segundo o escopo metodológico da pesquisa participante. Verificou-se que a empresa enfatizou a tecnologia na solução de problemas de gestão do conhecimento, em detrimento de questões comportamentais e culturais, caracterizando, assim, uma abordagem tipicamente mecanicista. Outros fatores interferiram no processo, tais como: baixo nível de participação e comunicação interna; uma cultura reativa do grupo pesquisado; e restrito compartilhamento de informações e socialização do conhecimento pelos pesquisadores

Genome Characterization and Spaciotemporal Dispersal Analysis of Bagaza Virus Detected in Portugal, 2021

Funding Information: This work received financial support from the Global Health and Tropical Medicine Center (which is funded through Fundação para a Ciência e a Tecnologia (FCT) contract UID/Multi/04413/2013). This research was also funded by FCT, Project UIDB/00276/2020 and LA/P/0059/2020-AL4AnimalS, and by the Interdisciplinary Research Center on Animal Health (CIISA), Faculty of Veterinary Medicine, University of Lisbon (Portugal). Finally, this research was also partially funded by the Interdisciplinary Research Center on Animal Health (Project CIISA-INOV 4/2021), Faculty of Veterinary Medicine, University of Lisbon (CIISA, FMV-UL) (Portugal). Publisher Copyright: © 2023 by the authors.In September 2021, Bagaza virus (BAGV), a member of the Ntaya group from the Flavivirus genus, was detected for the first time in Portugal, in the heart and the brain of a red-legged partridge found dead in a hunting ground in Serpa (Alentejo region; southern Portugal). Here we report the genomic characterization of the full-length sequence of the BAGV detected (BAGV/PT/2021), including phylogenetic reconstructions and spaciotemporal analyses. Phylogenies inferred from nucleotide sequence alignments, complemented with the analysis of amino acid alignments, indicated that the BAGV strain from Portugal is closely related to BAGV strains previously detected in Spain, suggesting a common ancestor that seems to have arrived in the Iberia Peninsula in the late 1990s to early 2000s. In addition, our findings support previous observations that BAGV and Israel turkey meningoencephalitis virus (ITV) belong to the same viral species.publishersversionpublishe

Simple method for establishing primary Leporidae skin fibroblast cultures

Research Areas: Cell BiologyCommercial hare and rabbit immortalized cell lines are extremely limited regarding the many species within the lagomorpha order. To overcome this limitation, researchers and technicians must establish primary cell cultures derived from biopsies or embryos. Among all cell types, fibroblasts are plastic and resilient cells, highly convenient for clinical and fundamental research but also for diagnosis, particularly for viral isolation. Here, we describe a fast and cheap method to produce primary fibroblast cell cultures from leporid species, using dispase II, a protease that allows dermal–epidermal separation, followed by a simple enzymatic digestion with trypsin. This method allows for the establishment of an in vitro cell culture system with an excellent viability yield and purity level higher than 85% and enables the maintenance and even immortalization of leporid fibroblastic cells derived from tissues already differentiated.info:eu-repo/semantics/publishedVersio

Avaliação de atendimento grupal para dependentes químicos em um centro de atenção psicossocial

O uso abusivo de álcool e drogas não é apenas um problema de saúde pública do Brasil, mas mundial. Apesar disso, poucos dependentes chegam aos serviços especializados de atendimento. Objetivo: Avaliar a eficácia dos atendimentos em grupos através de escalas de medida da NOC, em um CAPS ad. Pesquisa transversal, realizada em um CAPS ad do interior de Goiás, entre outubro de 2009 a fevereiro de 2010. Participaram 22 usuários do serviço. Coleta de dados através de escalas da NOC e análise pelo programa Excel. Pesquisa aprovada pelo Comitê de Ética da UnieVANGÉLICA, ofício no 190/2008. A maioria reconhece o risco e as consequências do uso contínuo e abusivo de álcool e múltiplas drogas; não estão em abstinência apesar de se considerarem aderidos ao programa. Os resultados mostram a importância de maiores reflexões sobre o enfoque terapêutico exclusivo na abstinência; adesão e eficácia do mesmo

Tracking the Origin of a Rabbit Haemorrhagic Virus 2 Outbreak in a Wild Rabbit Breeding Centre in Portugal; Epidemiological and Genetic Investigation

As key prey, the wild rabbit downsize constitutes a major drawback on the endangered Iberian lynx (Lynx pardinus) re-introduction in the Iberia. Several captive breeding units mostly located in Alentejo, endeavour the wild rabbit repopulation of depleted areas assigned for the lynx re-introduction. Here we report an RHDV2 outbreak that occurred in early 2016 in a wild rabbit captive breeding unit located in Barrancos municipality. The estimated mortality rate between March and April 2016 was approximately 8.67%. Anatomopathologic examination was carried out for 13 victimized rabbits. Molecular characterization was based on the complete vp60 capsid gene. The 13 rabbit carcasses investigated showed typical macroscopic RHD lesions testing positive to RHDV2- RNA. Comparison of the vp60 nucleotide sequences obtained from two specimens with others publically available disclosed similarities below 98.22% with RHDV2 strains originated in the Iberia and Azores and revealed that the two identical strains from Barrancos-2016 contain six unique single synonymous nucleotide polymorphisms. In the phylogenetic analysis performed, the Barrancos-2016 strains clustered apart from other known strains, meaning they may represent new evolutionary RHDV2 lineages. No clear epidemiological link could be traced for this outbreak where the mortalities were lower compared with previous years. Yet, network analysis suggested a possible connection between the missing intermediates from which the strains from Barrancos 2013, 2014 and 2016 have derived. It is therefore possible that RHDV2 has circulated endemically in the region since 2012, with periodic epizootic occurrences. Still, six years after its emergence in wild rabbits, RHDV2 continues to pose difficulties to the establishment of natural wild rabbit populations that are crucial for the self-sustainability of the local ecosystems

Leporids’ Emerging Diseases as a Threat to Biodiversity

Wild leporids have been gaining interest and prominence in the scientific and social community worldwide. While endangered of extinction in its native territory, the Iberian Peninsula, where it has a key role in the Mediterranean ecosystems, the European rabbit (Oryctolagus cuniculus) is considered a plague in Australia, due to the great economic and ecological consequences of its presence in the territories. The impact of viral diseases on the Leporidae family’ members, namely on the European rabbit, has been largely recognized worldwide since the early 50s, due to the emergence of myxomatosis and, from the mid-80s onwards, due to the emergence of rabbit haemorrhagic disease virus 1 and 2. More recently, in 2018, a recombinant myxoma virus emerged with the ability to infect and cause severe disease in the Iberian hare (Lepus ganatensis). Also, a new gammaherpesvirus was described in Iberian hares, associated with myxoma virus infections. In this chapter, we revise the main viral infectious treats to the native leporids of the Iberian Peninsula. The recovery of the European rabbit populations, as well as of other leporid species around the world, is currently a major challenge for the scientific and social communities and policy-makers. If we fail, the ripple effects on the trophic web will be so dramatic that are likely to be unrecoverable

Angina bullosa haemorrhagica:a 14-year multi-institutional retrospective study from Brazil and literature review

Angina bullosa haemorrhagica (ABH) is characterized by the recurrent appearance of blood blisters on the oral mucosa, mainly in adults' soft palate. In general, the blisters rupture spontaneously, lacking the necessity for biopsy. We report the clinical features of 23 ABH cases, emphasizing the clinical behavior and the management of these conditions. A retrospective descriptive cross-sectional study was performed. A total of 12,727 clinical records of oral and maxillofacial lesions from four dental services in Brazil were analyzed. Clinical data were collected from the clinical records and evaluated. The series comprised 12 males (52.2%) and 11 females (47.8%), with a mean age of 56.8?±?14.6?years (ranging: 24-82?years) and a 1.1:1 male-to-female ratio. Most of the lesions affected the soft palate (n = 15, 65.2%). Clinically, the lesions presented mainly as an asymptomatic (n = 17, 73.9%) blood-filled blister that ruptured after a few minutes or hours, leaving an erosion. The masticatory trauma was the most frequent triggering event. No patient had coagulation disorders. A biopsy was performed in only four cases (17.4%). Treatment was symptomatic with a favorable outcome. ABH is still poorly documented in the literature, and its etiology remains uncertain. ABH mainly affects the soft palate of elderly adults and has a favorable evolution in a few days. The therapeutic approach is often focused only on the relief of symptoms. However, it can share some clinical features with more serious diseases. Therefore, clinicians must recognize these lesions to avoid misdiagnosis