86 research outputs found
Photolysis of a caged peptide reveals rapid action of NSF prior to neurotransmitter release
Author Posting. © The Author(s), 2007. This is the author's version of the work. It is posted here by permission of National Academy of Sciences for personal use, not for redistribution. The definitive version was published in Proceedings of the National Academy of Sciences 105 (2008): 347-352, doi:10.1073/pnas.0707197105.The time at which the N-ethylmaleimide-sensitive factor (NSF) acts during
synaptic vesicle trafficking was identified by time-controlled perturbation of NSF
function with a photo-activatable inhibitory peptide. Photolysis of this caged
peptide in the squid giant presynaptic terminal caused an abrupt (0.2 s) slowing
of the kinetics of the postsynaptic current (PSC) and a more gradual (2-3 s)
reduction in PSC amplitude. Based on the rapid rate of these inhibitory effects
relative to the speed of synaptic vesicle recycling, we conclude that NSF
functions in reactions that immediately precede neurotransmitter release. Our
results indicate the locus of SNARE protein recycling in presynaptic terminals
and reveal a new target for rapid regulation of transmitter release.T.K. was supported by a Grass Fellowship in Neuroscience, an HFSP long-term
fellowship and the Feodor-Lynen Program of the Alexander von Humboldt
Foundation. Y.L. received a American Heart Association predoctoral fellowship.
The research also was supported by NIH NS-21624
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