Идентификация протеоформ в результатах 2D электрофореза по предсказанным значениям величины изоэлектрической точки

The possibility of identifying specific protein proteoforms with post-translational modifications (PTM) by analyzing two-dimensional (2D) gel electrophoresis maps and using the prediction of the isoelectric point of proteins (pI) has been investigated. The pI values were predicted using the pIPredict 3 program, supporting a wide range of chemical and post-translational modifications. Eleven 11 proteins (albumin, alpha-1-microglobulin, annexin A2, apolipoprotein E, gastric triacylglycerol lipase, mitochondrial isocitrate dehydrogenase, clusterin, plasmin, prothrombin, endoplasmic reticulum chaperone, S-adenosylmethionine synthase type 1) identified on six 2D electrophoresis maps were used as examples. Various options for selecting hypotheses are considered. These take into consideration the following available information about a particular protein: possible modification sites, processing features, variability of the amino acid composition. The obtained results indicate that the use of predicting the pI value for proteins with hypothetical PTMs can form a set of hypotheses about specific proteoform occurrence on 2D electrophoresis maps.В работе рассмотрена возможность идентификации конкретных протеоформ белка с посттрансляционными модификациями (ПТМ) по данным анализа карт двумерного (2D) гель-электрофореза с использованием предсказания величины изоэлектрической точки белков (pI). Для предсказания значений pI использовали программу pIPredict 3, которая поддерживает широкий спектр химических и посттрансляционных модификаций. В качестве конкретных примеров рассмотрены 11 белков (альбумин, альфа-1-микроглобулин, аннексин А2, аполипопротеин Е, желудочная триацилглицероллипаза, митохондриальная изоцитратдегидрогеназа, кластерин, плазмин, протромбин, шаперон эндоплазматического ретикулума, S-аденозилметионинсинтаза 1-го типа), идентифицированных на шести картах 2D электрофореза. Рассмотрены различные варианты подбора гипотез с учётом доступной информации о конкретном белке: возможных сайтов модификации, особенностей процессинга, вариабельности аминокислотного состава. Полученные результаты свидетельствуют о том, что использование предсказания величины pI для белков с гипотетическими ПТМ может сформировать набор гипотез о том, какие конкретно протеоформы наблюдаются на картах 2D электрофореза

Critical Analysis of Advertising Media Texts as a Way of the Development of Media Literacy of Adolescents

Today it is extremely important to prepare citizens especially the new generation for the perception of diverse information to teach them how to conceive the media information and to understand the effects of media influence on the psyche as well. Every child must acquire the mechanism of critical perception of the information, the ability to interpret, analyze and evaluate the media texts, to understand their essence, purpose, and reveal the hidden meaning and attempts for the mind manipulation. While educational institutions in Western countries (USA, GB, France, Australia, Canada, etc.) introduced educational subjects aimed at forming the basics of media literacy in the 1960’s and the 20 century, this work really started only within the last years in Ukraine.The theoretical aspects of the use of advertising as a way for developing the media literacy of adolescents are considered in the paper. A model for the development of media literacy through a critical analysis of media texts is proposed. The model consists of three stages: ad analysis, ad evaluation, creating for personal ad product. The model implies for developing the appropriate skills of critical thinking for each of the above mentioned stages. The author has carried out the pedagogical experiment for the purpose to approbate the model. 147 students (age is 12-17 years old) have taken a part in the experiment. The experimental group consists of 79 teenagers while the control group consists of 68 teenagers. The experimental group of approaches for the critical ad analysis was organized. As result of the experiment, teenagers have learnt how to define the ad language, how to analyze the ad influence on crowd, how to state the conclusions with regard to their own opinion of ad, how to make the examples with regard to the thinking manipulations by ad messages. The diagnostic cuts conducted again after the implementation of the proposed model of media literacy development, has demonstrated the following results: 65.8% of students with a developed level of cognitive component of media literacy were recorded in Experimental Group, which is 50.6% higher in comparison with data at the beginning of the experiment. This indicator has been increased by only 17.6% in Control Group. 29.1% of the students in Control Group with an average level were recorded. At the basic level of the formation of a critical understanding of the advertising media text after the experiment left 5.1% of adolescents of Experimental Group and 16.2% of adolescents of Control Group. The obtained results can be used in implementing the media education in other educational institutions. Keywords: media literacy, media education, critical analysis, advertising

Using the Simulation Modeling Methods for the Designing Real-Time Integrated Expert Systems

Certain theoretical and methodological problems of designing real-time dynamical expert systems, which belong to the class of the most complex integrated expert systems, are discussed. Primary attention is given to the problems of designing subsystems for modeling the external environment in the case where the environment is represented by complex engineering systems. A specific approach to designing simulation models for complex engineering systems is proposed and examples of the application of this approach based on the G2 (Gensym Corp.) tool system are described

Static and Dynamic Integrated Expert Systems: State of the Art, Problems and Trends

Systemized analysis of trends towards integration and hybridization in contemporary expert systems is conducted, and a particular class of applied expert systems, integrated expert systems, is considered. For this purpose, terminology, classification, and models, proposed by the author, are employed. As examples of integrated expert systems, Russian systems designed in this field and available to the majority of specialists are analyzed

Білінгвізм та інтерференція як тенденції мовного розвитку сучасного суспільства: проблеми та способи їх вирішення (Bilingualism and interference as language trends of modern society: problems and their solutions)

Стаття присвячена вивченню проблем білінгвізму та інтерференції в процесі розвитку сучасного суспільства. У результаті здійсненого дослідження встановлено, що у процесі міжкультурної/міжмовної комунікації неминуче має місце зіткнення двох мов, наслідком якого постає новий когнітивно-емоційний лінгвокультурний простір, проявом якого є інтерференція. У роботі запропоновано також варіанти вирішення проблеми інтерферованого мовлення. (The article is devoted to the study of bilingualism and interference problems in the development of modern society. The paper elaborates a theoretical background for the analysis of those two phenomena. As a result, the research has found that in the process of intercultural / interlingual communication there is an inevitable clash of two languages, the result of which is a new cognitive emotional language cultural space, which is a manifestation of interference. The paper has also proposed solutions to the problem of interfered speech. It has been given the available programs for foreign languages improvement as well as recommendations for future studying of these very problems.

Occupational exposure to metals and other elements in the tractor production

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