3,011 research outputs found
First report of Leptosphaeria biglobosa ‘brassicae’ as causal agent of phoma leaf spot in Brassica napus (Canola) in Argentina
Canola(Brassicanapus L.) is the second largest oilseed crop in the worldproviding 13% of the world´s oil supply. This crop has been grown in Argentinasince the 1930s, and the area devoted to its cultivation varies every year,reaching a maximum of 95000 Ha in the 2012/2013 growing season. Because of theoccurrence of optimal weather conditions and soils with high fertility, theaverage yield in this region is about 2000 kg/Ha. Phoma leaf spot and Phomastem canker are considered the most important and devastating diseases in Brassica napus andother Brassicaespecies [1]. In both cases, the causal agent is a complexof two closely related fungal species, Leptosphaeria maculans and L. biglobosa. In Argentina,the presence of L. maculans incanola plants was reported for the first time in 2004 [2], but the existence ofL.biglobosa has not been recorded so far. During the 2015/2016season, we collected several samples with typical Phoma leaf spot symptoms fromcanola plants growing in fields from the north and northeastern regions of the Buenos Aires province.The necrotic lesions were circular to irregularly oval, 8 to 15 mm in diameter,pale brown in the center, grayish green at the margin and characterized withthe presence of pycnidia. Several leaf pieces with lesions were rinsed twicewith deionized sterile water and placed in a humid chamber (90 mm diameterPetri dish with a layer of filter paper soaked in deionized sterile water) during2-3 days to induce the pycnidia to exude cirri of conidia. After this period,one cirrus per sample was transferred onto PDA plates supplemented withantibiotics (15 mg/L streptomycin, 15 mg/L gentamicin and 12 mg/L tetracycline)using an inoculation needle under stereoscopic microscope. Thus, severalisolates were obtained, some of them showing rapid mycelial growth rate andpigment production on PDA medium, as showed by the isolate Tapidor of L. biglobosa thatwe used as control (kindly provided by Professor Bruce Fitt, University ofHertfordshire-UK). In order to confirm the identity of these isolates, a PCRassay using genomic DNA as template was performed to distinguish L. maculans from L. biglobosa withthe species-specific primers LmacR, LmacF,and LbigF ina three-primers strategy described by Gaetan (2005)[3]. These reactions gave a444-bp amplicon as expected for L. biglobosa ´brassicae´.In addition, these results were confirmed by sequencing the nuclear ribosomalinternal transcribed spacer (ITS) region, which showed a 99% of identity withthe sequence of L. biglobosa ´brassicae´at the GenBank database (FO905468). L. biglobosa isolateswere then tested for pathogenicity on the canola cultivars Westar and Bioaureo2286 (Nuseed). With this purpose, cotyledons of 10-day-old seedlings werepricked with a needle, and each wound inoculated with 10 μl ofa conidial suspension (107 42conidia/ml). Sterilized distilled water was used as control. Developing primaryleaves were removed every 2-3 days in order to ensure that cotyledons continueto expand. Fourteen days after inoculation, irregular and brown necroticlesions were visible at the site of inoculation. These cotyledons were detachedand placed in a humid chamber to induce pycnidia formation. After three dayscirri of conidia were transferred to a plate with PDA supplemented with antibioticsas mentioned above. The identity of these isolates of L. biglobosa wereconfirmed by pigment production on PDA medium and by PCR assay usingspecies-specific primers. To our knowledge, this is the first report of L. biglobosa ´brassicae´as a pathogen of canola in Argentina. This finding shows that in Argentina´scanola cropping areas not 50 only L. maculans but alsoL.biglobosa are the causal agents of Phoma leaf spot disease.Fil: Rossi, Franco Rubén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Romero, Fernando Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Garriz, Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Ruiz, Oscar Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentin
Evaluación del hemograma ferritina y hierro sérico de mujeres en edad reproductiva. Hospital Nacional Dos de Mayo. Lima-Perú 2012
Publicación a texto completo no autorizada por el autorDetermina la prevalencia de anemia y deficiencia de hierro en mujeres de edad fértil (MEF) que acuden a consultorios externos de planificación familiar del Hospital Nacional Dos de Mayo de Lima-Perú. Se realiza un estudio descriptivo y transversal, durante los meses de junio a diciembre del 2012, se incluyen mujeres de entre 18 y 49 años que voluntariamente aceptaron participar en el estudio. Se realiza estadística univariada y bivariada, los cálculos son realizados con un nivel de confianza del 95%. Se incluyen un total de 307 MEF, el promedio de hemoglobina es de 11,8±1.7 g/dL, siendo la prevalencia de anemia del 38% (IC95% 33-44). La anemia es leve en el 11,4% de los casos, moderada en el 24,1% y severa en el 2,9%. El promedio de ferritina sérica es de 15,9±9,7 ng/ml, estando baja en el 72% de los casos. El 97,4% de las mujeres con anemia presentan ferritina sérica baja, así mismo se aprecia que 56,1% de las mujeres sin anemia presentan ferritina sérica baja. Concluye que en esta población existe una gran prevalencia de anemia y deficiencia de hierro, superior a lo reportado a nivel nacional, lo que podría indicar grandes diferencias en la prevalencia de anemia de acuerdo a la procedencia de las MEF.Tesi
Una escuela abierta. Una investigación sobre nuevos modelos escolares
El presente trabajo realiza un ejercicio de proyecto de Escuela Infantil y de Primaria en Zaragoza que responde a las problemáticas actuales. Una escuela abierta, que se relaciona de manera estrecha con la naturaleza, explorando nuevos espacios para la docencia. <br /
Polyamine-mediated mechanisms contribute to oxidative stress tolerance in Pseudomonas syringae
Bacterial phytopathogens living on the surface or within plant tissues may experience oxidative stress because of the triggered plant defense responses. Although it has been suggested that polyamines can defend bacteria from this stress, the mechanism behind this action is not entirely understood. In this study, we investigated the effects of oxidative stress on the polyamine homeostasis of the plant pathogen Pseudomonas syringae and the functions of these compounds in bacterial stress tolerance. We demonstrated that bacteria respond to H2O2 by increasing the external levels of the polyamine putrescine while maintaining the inner concentrations of this compound as well as the analogue amine spermidine. In line with this, adding exogenous putrescine to media increased bacterial tolerance to H2O2. Deletion of arginine decarboxylase (speA) and ornithine decarboxylate (speC), prevented the synthesis of putrescine and augmented susceptibility to H2O2, whereas targeting spermidine synthesis alone through deletion of spermidine synthase (speE) increased the level of extracellular putrescine and enhanced H2O2 tolerance. Further research demonstrated that the increased tolerance of the ΔspeE mutant correlated with higher expression of H2O2-degrading catalases and enhanced outer cell membrane stability. Thus, this work demonstrates previously unrecognized connections between bacterial defense mechanisms against oxidative stress and the polyamine metabolism.Fil: Solmi, Leandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Rossi, Franco Rubén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Romero, Fernando Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Bach Pages, Marcel. University of Oxford; Reino UnidoFil: Preston, Gail M.. University of Oxford; Reino UnidoFil: Ruiz, Oscar Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Gárriz, Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentin
Inferring the Significance of the Polyamine Metabolism in the Phytopathogenic Bacteria Pseudomonas syringae: A Meta-Analysis Approach
To succeed in plant invasion, phytopathogenic bacteria rely on virulence mechanisms to subvert plant immunity and create favorable conditions for growth. This process requires a precise regulation in the production of important proteins and metabolites. Among them, the family of compounds known as polyamines have attracted considerable attention as they are involved in important cellular processes, but it is not known yet how phytopathogenic bacteria regulate polyamine homeostasis in the plant environment. In the present study, we performed a meta-analysis of publicly available transcriptomic data from experiments conducted on bacteria to begin delving into this topic and better understand the regulation of polyamine metabolism and its links to pathogenicity. We focused our research on Pseudomonas syringae, an important phytopathogen that causes disease in many economically valuable plant species. Our analysis discovered that polyamine synthesis, as well as general gene expression activation and energy production are induced in the early stages of the disease. On the contrary, synthesis of these compounds is inhibited whereas its transport is upregulated later in the process, which correlates with the induction of virulence genes and the metabolism of nitrogen and carboxylic acids. We also found that activation of plant defense mechanisms affects bacterial polyamine synthesis to some extent, which could reduce bacterial cell fitness in the plant environment. Furthermore, data suggest that a proper bacterial response to oxidative conditions requires a decrease in polyamine production. The implications of these findings are discussed.Fil: Solmi, Leandro. Universidad Nacional de San Martin. Instituto Tecnologico de Chascomus. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - la Plata. Instituto Tecnologico de Chascomus.; ArgentinaFil: Rosli, Hernan Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; ArgentinaFil: Pombo, Marina Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; ArgentinaFil: Stalder, Santiago. Universidad Nacional de San Martin. Instituto Tecnologico de Chascomus. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - la Plata. Instituto Tecnologico de Chascomus.; ArgentinaFil: Rossi, Franco Rubén. Universidad Nacional de San Martin. Instituto Tecnologico de Chascomus. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - la Plata. Instituto Tecnologico de Chascomus.; ArgentinaFil: Romero, Fernando Matias. Universidad Nacional de San Martin. Instituto Tecnologico de Chascomus. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - la Plata. Instituto Tecnologico de Chascomus.; ArgentinaFil: Ruiz, Oscar Adolfo. Universidad Nacional de San Martin. Instituto Tecnologico de Chascomus. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - la Plata. Instituto Tecnologico de Chascomus.; ArgentinaFil: Gárriz, Andrés. Universidad Nacional de San Martin. Instituto Tecnologico de Chascomus. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - la Plata. Instituto Tecnologico de Chascomus.; Argentin
Norharmane rhenium(I) polypyridyl complexes: synthesis, structural and spectroscopic characterization
Two novel Re(I) complexes with the general formula fac-[Re(CO)3(L)(nHo)]CF33SO3, where L = 2,2’-bipyridine (bpy) or 1,10 phenanthroline (phen) and nHo (9H-pyrido[3,4-b]indole; norharmane) have been synthesized. The Re(I)–nHo complexes were characterized by structural X-ray diffraction, 1H and 13C NMR, UV-vis absorption and FT-IR spectroscopy, and by a combination of two mass spectrometry techniques, namely ESI-MS and UV-MALDI-MS. All characterizations showed that nHo is coordinated to the metal atom by the pyridine nitrogen of the molecule. X-ray structural analysis revealed that the crystal lattices for both complexes are further stabilized by a strong >N–H⋯O bond between the pyrrole NH group of the pyridoindole ligand and one oxygen atom of the trifluoromethanesulfonate counter-ion. Ground state geometry optimization by DFT calculations showed that in fluid solution the nHo ligand may rotate freely. The nature of the electronic transitions of Re(CO)3(bpy)(nHo)⁺ were established by TD-DFT calculations. The set of the most important electronic transitions present in this complex are comprised of π → π* electronic transitions centered on bpy and nHo moieties, LLCTnHo→COs, MLLCTRe(CO)3→bpy and LLCTnHo→bpy transitions. Additionally, TD-DFT calculations predict the existence of another two intense MLLCTRe(CO)3→nHo electronic transitions. Calculated UV-vis absorption spectra are in good agreement with the corresponding experimental data for the bpy-containing complex.Facultad de Ciencias ExactasInstituto de Investigaciones Fisicoquímicas Teóricas y AplicadasInstituto de Física La Plat
Calidad de agua en el sitio piloto cuenca del arroyo Garupá, Misiones
El objetivo del trabajo fue evaluar la calidad de agua empleada por productores del Sitio Piloto Cuenca Arroyo Garupá, y realizar recomendaciones en la gestión del recurso de acuerdo al uso, con énfasis en consumo humano y riego.EEA Cerro AzulFil: Iwasita, Bárbara Eloisa. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Cerro Azul; ArgentinaFil: Albarracín Franco, Silvia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Cerro Azul; ArgentinaFil: Paniagua, Oscar. Ministerio de Ecología y R.N.R., Provincia de Misiones; Argentina.Fil: Capuzzi, Gabriel Francisco. Parque Federal Campo San Juan (A.P.N.), M.E.yR.N.R., Santa Ana, Misiones; Argentina.Fil: Torresin, Jerónimo. Fundación Temaikén, Misiones; Argentina.Fil: Ruiz Diaz, Gabriel. Fundación Temaikén, Misiones; Argentina
Instalación y configuración de Zentyal 6.2 para la implementación de servicios de infraestructura IT
En este informe se realiza la instalación y configuración de Zentyal server 6.2 como sistema operativo base para la infraestructura IT de mayor nivel para intranet y extranet de instituciones complejas y se realiza toda la implementación a detalle de servicios como servidor DHCP, servidor DNS, controlador de dominio, proxy no transparente, cortafuego, VPN, file Server y print Server para tener acceso a una estación de trabajo a través del controlador de dominio LDAP a los servicios de carpetas compartidas e impresoras.This report performs the installation and configuration of Zentyal server 6.2 as the base operating system for the higher-level IT infrastructure for intranet and extranet of complex institutions and performs all the detailed implementation of services such as DHCP server, DNS server, controller domain, non-transparent proxy, firewall, VPN, file server, and print server to access a workstation through the LDAP domain controller to printer and shared folder services
Technical note: Productive variables of Pelibuey lambs and ewes induced to estrus with exogenous hormones and with different types of suckling
With the objective of analyzing the productive and reproductive response of lambs and ewes subjected to induced or synchronized estrus and to three different types of suckling, 60 Pelibuey ewes and their lambs were randomly assigned, at seven days postpartum, to one of three groups (n = 20); continuous suckling (AC), restricted suckling (AR) and early weaning (DP). The animals were weighed weekly from birth until the eighth week. At 30 d postpartum, the hormonal treatment applied was intravaginal progestagen (P4) plus 0.05 mg of PGF2á and 300 UI of eCG two days before the removal of P4. The percentage of estrus was higher (95%) in DP and AR groups compared with AC (60%; P < 0.05), as was fertility (DP, 80%; AR 75%) versus 40% in AC (P < 0.05), and prolificacy (lambs/ewe, 1.87 and 1.5; P < 0.05). Weight of lambs at 8 wk of age was higher for AC (16.8) and AR (16.5) than for DP (11.9 kg; P < 0.05). In conclusion, the restriction of suckling and early weaning, improved the reproductive efficiency in Pelibuey ewes. However, DP reduced weight gain of the lambs and increased their mortality rate relative to AC and AR
Morphological characteristics of bone marrows in patients with SARS-CoV-2 infection from Hospital 2 de Mayo, Peru
Introducción: A nivel medular, el SARS-COV2 puede comprometer la hematopoyesis, manifestándose con citopenias y solo se cuenta con estudios realizados en autopsias. Objetivo: describir las características morfológicas de las médulas óseas de los pacientes hospitalizados por neumonía por COVID-19 en el Hospital Nacional “Dos de Mayo”. El estudio: Estudio transversal retrospectivo llevado a cabo en pacientes con diagnóstico de COVID-19. Las lecturas de las médulas óseas fueron confirmadas por un hematólogo entrenado. Se utilizó estadística descriptiva para las variables cuantitativa y cualitativas. Hallazgos: Se incluyeron 30 pacientes. Los hallazgos más frecuentes fueron: macrófagos con citofagocitosis (87%), hiperplasia con detención en la maduración de los progenitores mieloides en el (70%). El 87% de las muestras presentó eosinofilia. En el 57% de las muestras se observó ninguna o poca evidencia de formación plaquetaria. En el 40% se encontró 6% de células plasmáticas. Conclusión: La infección por SARS-COV2 puede generar alteraciones medulares
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