Diversity and enumeration of halophilic and alkaliphilic bacteria in Spanish-style green table-olive fermentations

© 2015 Elsevier Ltd. The presence and enumeration of halophilic and alkaliphilic bacteria in Spanish-style table-olive fermentations was studied. Twenty 10-tonne fermenters at two large manufacturing companies in Spain, previously studied through both culture dependent and independent (PCR-DGGE) methodologies, were selected. Virtually all this microbiota was isolated during the initial fermentation stage. A total of 203 isolates were obtained and identified based on 16S rRNA gene sequences. They belonged to 13 bacterial species, included in 11 genera. It was noticeable the abundance of halophilic and alkaliphilic lactic acid bacteria (HALAB). These HALAB belonged to the three genera of this group: Alkalibacterium, Marinilactibacillus and Halolactibacillus. Ten bacterial species were isolated for the first time from table olive fermentations, including the genera Amphibacillus, Natronobacillus, Catenococcus and Streptohalobacillus. The isolates were genotyped through RAPD and clustered in a dendrogram where 65 distinct strains were identified. Biodiversity indexes found statistically significant differences between both patios regarding genotype richness, diversity and dominance. However, Jaccard similarity index suggested that the halophilic/alkaliphilic microbiota in both patios was more similar than the overall microbiota at the initial fermentation stage. Thus, up to 7 genotypes of 6 different species were shared, suggesting adaptation of some strains to this fermentation stage. Morisita-Horn similarity index indicated a high level of codominance of the same species in both patios. Halophilic and alkaliphilic bacteria, especially HALAB, appeared to be part of the characteristic microbiota at the initial stage of this table-olive fermentation, and they could contribute to the conditioning of the fermenting brines in readiness for growth of common lactic acid bacteria.This research was funded by the Spanish Ministry of Science and Innovation (MICINN), through Projects AGL2009-07861 and AGL2012-33400, and by the Junta de Andalucía Excellence Projects AGR-04621 and AGR-07345. All these projects included FEDER funds. AMB was the recipient of a post-doctoral grant awarded by the Junta de Andalucía as part of the Project AGR-07345. HLP was the recipient of a contract funded by the Spanish Ministry of Economy and Competitiveness as part of the Project AGL2012-33400. We want to express our most sincere gratitude to Juan Carlos Roldán, from JOLCA S.A., and Antonio Martín and Marta Sánchez, from GOYA en España S.A.U., for their invaluable collaboration in this study.Peer Reviewe

Editorial: Emotional and social value of organizations

This study was supported by the University of the Basque Country under Grant US20/11, the Consejeria de Educacion, Cultura y Deportes (JCCM), and the European Regional Development Fund (ERDF) under Grant SBPLY/21/180501/000192

Quorum-sensing control of bacteriocin production in olive fermentations

Bacteriocin production in many lactic acid bacteria is controlled by specific peptides called autoinducers (AIPs) via a sophisticated mechanism which senses cell density and is known as quorum sensing (QS).Peer Reviewe

Microbial community and volatilome changes in brines along the spontaneous fermentation of Spanish-style and natural-style green table olives (Manzanilla cultivar)

9 Páginas.-- 5 Figuras.-- 1 TablaMicrobial community and volatilome of brines were monitored during the spontaneous fermentations of Spanish-style and Natural-style green table olives from Manzanilla cultivar. Fermentation of olives in the Spanish style was carried out by lactic acid bacteria (LAB) and yeasts, whereas halophilic Gram-negative bacteria and archaea, along with yeasts, drove the fermentation in the Natural style. Clear differences between the two olive fermentations regarding physicochemical and biochemical features were found. Lactobacillus, Pichia, and Saccharomyces were the dominant microbial communities in the Spanish style, whereas Allidiomarina, Halomonas, Saccharomyces, Pichia, and Nakazawaea predominated in the Natural style. Numerous qualitative and quantitative differences in individual volatiles between both fermentations were found. The final products mainly differed in total amounts of volatile acids and carbonyl compounds. In addition, in each olive style, strong positive correlations were found between the dominant microbial communities and various volatile compounds, some of them previously reported as aroma-active compounds in table olives. The findings from this study provide a better understanding of each fermentation process and may help the development of controlled fermentations using starter cultures of bacteria and/or yeasts for the production of high-quality green table olives from Manzanilla cultivar.This work was funded by the Junta de Andalucia (project P20-00071) and the Spanish Government (grant number PID2020-116314RB-I00). These projects included European Regional Development Funds (ERDF).Peer reviewe

Physiological Responses at 15 Minutes of Recovery after a Session of Functional Fitness Training in Well-Trained Athletes

The aim of this study was to analyse muscle fatigue and metabolic stress at 15 min of recovery after performing two independent sessions of functional fitness training (FFT): a session of strength functional fitness training (FFTstrength) and a session of endurance functional fitness training (FFTendurance). Methods: eighteen well-trained men conducted two protocols, separated by one week of rest: FFTstrength (3 sets of 21, 15 and 9 repetitions of Thruster with bar + Pull ups) and FFTendurance (3 sets × (30 kcal rowing + 15 kcal assault air bike)). Neuromuscular fatigue and metabolic stress were measured right before, right after and at 10 and 15 min after completing the FFT workout, as well as the mean heart rate (HRmean) and the rating of perceived exertion (RPE) at the end of the FFT. Results: FFTendurance recovered the velocity loss values after 15 min of recovery. On the other hand, FFTstrength only recovered velocity in the 1 m·s−1 Tests in squat (SQ), since the velocity levels were 7% lower in the 1 m·s−1 Tests in military press exercise (MP) after 15 min. Conclusions: These data indicate that there are specific recovery patterns not only as a function of the exercise and the body regions involved, but also regarding the recovery of neuromuscular and metabolic factors, since both FFT workouts obtained high blood lactate concentrations.Sección Deptal. de Radiología, Rehabilitación y Fisioterapia (Enfermería)Fac. de Enfermería, Fisioterapia y PodologíaTRUEAlfonso X el Sabio University Foundationpu

Effects of Creatine Supplementation after 20 Minutes of Recovery in a Bench Press Exercise Protocol in Moderately Physically Trained Men

The aims of this study were to analyse the effect of creatine supplementation on the performance improvement in a bench pressing (BP) strength test of muscle failure and to evaluate muscle fatigue and metabolic stress 20 min after the exercise. Methods: Fifty young and healthy individuals were randomly assigned to a creatine group (n = 25) or a placebo group (n = 25). Three exercise sessions were carried out, with one week of rest between them. In the first week, a progressive load BP test was performed until the individuals reached the one repetition maximum (1RM) in order to for us obtain the load-to-velocity ratio of each participant. In the second week, the participants conducted a three-set BP exercise protocol against 70% 1RM, where they performed the maximum number of repetitions (MNR) until muscle failure occurred, with two minutes of rest between the sets. After one week, and following a supplementation period of 7 days, where half of the participants consumed 0.3 g·kg−1·day−1 of creatine monohydrate (CR) and the other half consumed 0.3 g·kg−1·day−1 of placebo (PLA, maltodextrin), the protocol from the second week was repeated. After each set, and up to 20 min after finishing the exercise, the blood lactate concentrations and mean propulsive velocity (MPV) at 1 m·s−1 were measured. Results: The CR group performed a significantly higher number of repetitions in Set 1 (CR = 14.8 repetitions, PLA = 13.6 repetitions, p = 0.006) and Set 2 (CR = 8 repetitions, PLA = 6.7 repetitions, p = 0.006) after supplementation, whereas no significant differences were seen in Set 3 (CR = 5.3 repetitions, PLA = 4.7 repetitions, p = 0.176). However, there was a significant increase in blood lactate at minute 10 (p = 0.003), minute 15 (p = 0.020), and minute 20 (p = 0.015) after the exercise in the post-supplementation period. Similarly, a significant increase was observed in the MPV at 1 m·s−1 in the CR group with respect to the PLA group at 10, 15, and 20 min after the exercise. Conclusions: Although the creatine supplementation improved the performance in the strength test of muscle failure, the metabolic stress and muscle fatigue values were greater during the 20 min of recovery

Sociodemographic, clinical and laboratory factors on admission associated with COVID19 mortality in hospitalized patients: A retrospective observational study

Background To identify and quantify associations between baseline characteristics on hospital admission and mortality in patients with COVID-19 at a tertiary hospital in Spain. Methods and findings This retrospective case series included 238 patients hospitalized for COVID-19 at Hospital Universitario Clı´nico San Cecilio (Granada, Spain) who were discharged or who died. Electronic medical records were reviewed to obtain information on sex, age, personal antecedents, clinical features, findings on physical examination, and laboratory results for each patient. Associations between mortality and baseline characteristics were estimated as hazard ratios (HR) calculated with Cox regression models. Series mortality was 25.6%. Among patients with dependence for basic activities of daily living, 78.7% died, and among patients residing in retirement homes, 80.8% died. The variables most clearly associated with a greater hazard of death were age (3% HR increase per 1-year increase in age; 95%CI 1–6), diabetes mellitus (HR 2.42, 95%CI 1.43–4.09), SatO2/ FiO2 ratio (43% HR reduction per 1-point increase; 95%CI 23–57), SOFA score (19% HR increase per 1-point increase, 95%CI 5–34) and CURB-65 score (76% HR increase per 1- point increase, 95%CI 23–143). Conclusions The patients residing in retirement homes showed great vulnerability. The main baseline factors that were independently associated with mortality in patients hospitalized for COVID-19 were older age, diabetes mellitus, low SatO2/FiO2 ratio, and high SOFA and CURB-65 scores.Fondos Estructurales de la Union Europea (FEDER)Unit of Excellence on Exercise and Health (UCEES), University of Granad

Recommendations for screening, monitoring, prevention, and prophylaxis of infections in adult and pediatric patients receiving CAR T-cell therapy : a position paper

Chimeric antigen receptor (CAR) T-cell therapy is one of the most promising emerging treatments for B-cell malignancies. Recently, two CAR T-cell products (axicabtagene ciloleucel and tisagenlecleucel) have been approved for patients with aggressive B-cell lymphoma and acute lymphoblastic leukemia; many other CAR-T constructs are in research for both hematological and non-hematological diseases. Most of the patients receiving CAR-T therapy will develop fever at some point after infusion, mainly due to cytokine release syndrome (CRS). The onset of CRS is often indistinguishable from an infection, which makes management of these patients challenging. In addition to the lymphodepleting chemotherapy and CAR T cells, the treatment of complications with corticosteroids and/or tocilizumab increases the risk of infection in these patients. Data regarding incidence, risk factors and prevention of infections in patients receiving CAR-T cell therapy are scarce. To assist in patient care, a multidisciplinary team from hospitals designated by the Spanish Ministry of Health to perform CAR-T therapy prepared these recommendations. We reviewed the literature on the incidence, risk factors, and management of infections in adult and pediatric patients receiving CAR-T cell treatment. Recommendations cover different areas: monitoring and treatment of hypogammaglobulinemia, prevention, prophylaxis, and management of bacterial, viral, and fungal infections as well as vaccination prior and after CAR-T cell therapy

La investigación en la Universidad

[Actas de:] "La Universidad española a examen: Jornadas sobre la Investigación en la Universidad" (25 y 26 de febrero de 1999: Universidad Carlos III de Madrid)Con motivo del X aniversario de la Universidad Carlos III de Madrid, el Instituto Antonio de Nebrija de Estudios sobre la Universidad organizó durante los días 25 y 26 de febrero de 1999 un encuentro titulado La universidad española a examen: Jornadas sobre investigación en la Universidad. En el encuentro celebrado participaron representantes de distintos ámbitos científicos, con el fin de ofrecer una visión más completa del panorama actual de la investigación en la Universidad, dejando constancia de las opiniones de la empresa, las universidades, las instituciones políticas, los organismos públicos de investigación. Este libro recoge sus resultados.Prólogo / Carmen Merino. -- Programa. -- Presentación / Gregorio Peces-Barba. -- La Investigación en la Universidad. Una opinion / César Nombela. -- Los Institutos Universitarios como estructuras organizativas de la investigación / José Manuel González Ros. -- Valoración de la investigación en la universidad española / Pedro Ramos Castellanos. -- Universidad e investigación. Por la diversidad y la rebeldía / Juan Urrutia. -- La universidad española, una fuente de tecnología necesaria para la empresa / Juan Mulet Meliá. -- Relaciones universidad y empresa. Una aportación empresarial / Ángel Martínez Román. -- Algunas reflexiones sobre el III Plan Regional de Investigación Científica e Innovación Tecnológica / Enrique Otero. -- Centros de Apoyo a la Investigación (CAI) / Juan Carlos Prieto Villapún. -- Infraestructuras científicas necesarias en la Comunidad de Madrid / Rodolfo Miranda. -- Importancia de los recursos humanos en relación con la I+D en Universidades / José Luis Sotelo. -- Investigación y Universidad / Alfonso Ruiz Miguel. -- Sobre el actual sistema de financiación de I+D / José Ramón Casar Corredera. -- Universidad e investigación: Hacia unas nuevas referencias en el contexto del Sistema Ciencia-Tecnología-Sociedad / Francisco Marcellán. -- Políticas de I+D en la Comunidad de Madrid / Francisco Rubia Vila. -- Perspectivas del Nuevo Plan Nacional de I+D / Javier Pascual Casado. -- Primer debate. Moderador Enrique Villalba. -- Segundo debate. Moderadora Adela Mora. -- Tercer debate. Moderador Francisco Marcellán. -- Clausura / Francisco Marcellán. -- Epílogo / Clara Eugenia Garcí