Managing low productive forests at catchment scale: Considering water, biomass and fie risk to achieve economic feasibility

[EN] Semi-arid forests are water limited environments considered as low-productive. As a result, these forests usually end up unmanaged and abandoned, with the subsequent wild fire risk increasing, water yield decreasing and a general diminishing of the forest resilience. Hydrological-oriented silviculture could be a useful alternative that increases management possibilities by combining forest productivity and water yield. However, the slight water yield increase after forest management together with the low forest productivity, could make this option insufficient for semi-arid forests, and other goods and services should be included and quantified. In this sense, the present study analyzes to what extent semi-arid forest management for water yield results effective and profitable at catchment scale, and how does it improve when it is combined with other benefits such as biomass production and fire risk diminishing. To that end, the effects of forest management of semi-arid Aleppo pine post-fire regeneration stands are analyzed in terms of water yield (TETIS-VEG model), fire risk (KDBY index and FARSITE) and biomass production, at catchment scale. Regarding to water yield, the results confirmed the slight effect of forest management on its increase (average increase of 0.27 +/- 0.29 mm yr(-1)), at the same time that highlighted the role of the upper catchment area as an important water contributor. The management produced 4161.6 Mg of biomass, and decreased in 27 +/- 17% and 25.6 +/- 14.1% the fire risk and fire propagation, respectively. Finally, a simple economic estimation of the management profitability is carried out by means of comparing the Benefit/Cost ratio of the managed and unmanaged scenarios. Both scenarios were always above the unity when just considering water as benefit, although the unmanaged scenario produced a higher ratio, as no management costs are expended. Contrarily, when wildfire was also included into the evaluation, the situation is overturned for wildfires equal or higher than 1.5 day duration, where the forest management is shown as the most convenient alternative.This study is a component of the research projects: INTEGRA (CGL2011-28776-0O2), E-HIDROMED (CGL2014-58127-C3) and CEHYRFO-MED (CGL2017-86839-C3-2-R) funded by the Spanish Ministry of Science and Innovation and FEDER funds, and LIFE17 CCA/ES/000063 RESILIENTFORESTS. The authors are grateful to the Valencia Regional Government (CMAAUV, Generalitat Valenciana), the VAERSA staff, the Natural Park staff and the communal authority of Serra (specially Juanjo Mayans) for their support and allowing the use of the Natural Park experimental forest.González Sanchís, MDC.; Ruiz Perez, G.; Garcia-Prats, A.; Campo García, ADD.; Francés, F.; Lull, C. (2019). Managing low productive forests at catchment scale: Considering water, biomass and fie risk to achieve economic feasibility. Journal of Environmental Management. 231:653-665. https://doi.org/10.1016/j.jenvman.2018.10.078S65366523

New pathogenic variant in DLX5: New clues for a clinical spectrum from split-hand-foot malformation to fibular aplasia, tibial campomelia and oligosyndactyly

Introduction: FATCO (Fibular Aplasia, Tibial Campomelia and Oligosyndactyly) is a very infrequent skeletal dysplasia classified within the limb hypoplasia-reduction defects group whose genetic cause has not yet been identified. The advent of next-generation sequencing is enabling the diagnosis of diseases with no previously known genetic cause.Methods: We performed a thorough autopsy on a fetus whose pregnancy was legally terminated due to severe malformations detected by ultrasound. A trio exome was run to identify the genetic cause and risk of recurrence. Previous literature of similar cases was systematically searched.Results: Anatomopathological analyses revealed complete fibular aplasia, shortened and campomelic tibia, absent ankle joint, club right foot and a split foot malformation, leading to the diagnosis of FATCO. Exome sequencing showed that the female fetus carried a de novo nonsense variant in DLX5. The literature search permitted the collection of information on 43 patients with FATCO, the majority of whom were males diagnosed postnatally. In most cases, lower limbs were affected exclusively, but in 39.5% of cases the upper limbs were also affected.Conclusion: The pathologies associated with DLX5 variants encompass a wide spectrum of manifestations ranging from abnormalities exclusively in the hands and feet to long bones such as the tibia and fibula

Daratumumab in combination with urelumab to potentiate anti-myeloma activity in lymphocytedeficient mice reconstituted with human NK cells

Daratumumab is an anti-CD38 fully human IgG1 mAb approved for multiple myeloma treatment. One of the proposed mechanisms of action is the induction of antibody-dependent cellular cytotoxicity (ADCC) mediated by NK cells. NK cells acquire surface CD137 expression in the presence of solid-phase-attached daratumumab and when encountering a daratumumab-coated CD38+ tumor cell line. In this setting, addition of the agonist anti-CD137 mAb urelumab enhances NK-cell activation increasing CD25 expression and IFNɣ production. However, in vitro ADCC is not increased by the addition of urelumab both in 4h or 24h lasting experiments. To study urelumab-increased daratumumab-mediated ADCC activity in vivo, we set up a mouse model based on the intravenous administration of a luciferase-transfected multiple myeloma cell line of human origin, human NK cells and daratumumab to immuno-deficient NSG mice. In this model, intravenous administration of urelumab 24h after daratumumab delayed tumor growth and prolonged mice survival

A randomized phase II clinical trial of dendritic cell vaccination following complete resection of colon cancer liver metastasis

Surgically resectable synchronic and metachronic liver metastases of colon cancer have high risk of relapse in spite of standard-of-care neoadjuvant and adjuvant chemotherapy regimens. Dendritic cell vaccines loaded with autologous tumor lysates were tested for their potential to avoid or delay disease relapses (NCT01348256). Patients with surgically amenable liver metastasis of colon adenocarcinoma (n = 19) were included and underwent neoadjuvant chemotherapy, surgery and adjuvant chemotherapy. Fifteen patients with disease-free resection margins were randomized 1:1 to receive two courses of four daily doses of dendritic cell intradermal vaccinations versus observation. The trial had been originally designed to include 56 patients but was curtailed due to budgetary restrictions. Follow-up of the patients indicates a clear tendency to fewer and later relapses in the vaccine arm (median disease free survival –DFS-) 25.26 months, 95% CI 8. 74-n.r) versus observation arm (median DFS 9.53 months, 95% CI 5.32–18.88)

Immunotherapeutic effects of intratumoral nanoplexed poly I:C

Poly I:C is a powerful immune adjuvant as a result of its agonist activities on TLR-3, MDA5 and RIG-I. BO-112 is a nanoplexed formulation of Poly I:C complexed with polyethylenimine that causes tumor cell apoptosis showing immunogenic cell death features and which upon intratumoral release results in more prominent tumor infiltration by T lymphocytes. Intratumoral treatment with BO-112 of subcutaneous tumors derived from MC38, 4 T1 and B16- F10 leads to remarkable local disease control dependent on type-1 interferon and gamma-interferon. Some degree of control of non-injected tumor lesions following BO-112 intratumoral treatment was found in mice bearing bilateral B16-OVA melanomas, an activity which was enhanced with co-treatment with systemic anti-CD137 and anti-PD-L1 mAbs. More abundant CD8+ T lymphocytes were found in B16-OVA tumor-draining lymph nodes and in the tumor microenvironment following intratumoral BO-112 treatment, with enhanced numbers of tumor antigen-specific cytotoxic T lymphocytes. Genome-wide transcriptome analyses of injected tumor lesions were consistent with a marked upregulation of the type-I interferon pathway. Inspired by these data, intratumorally delivered BO-112 is being tested in cancer patients (NCT02828098)

On the Use of Unmanned Aerial Systems for Environmental Monitoring

[EN] Environmental monitoring plays a central role in diagnosing climate and management impacts on natural and agricultural systems; enhancing the understanding of hydrological processes; optimizing the allocation and distribution of water resources; and assessing, forecasting, and even preventing natural disasters. Nowadays, most monitoring and data collection systems are based upon a combination of ground-based measurements, manned airborne sensors, and satellite observations. These data are utilized in describing both small-and large-scale processes, but have spatiotemporal constraints inherent to each respective collection system. Bridging the unique spatial and temporal divides that limit current monitoring platforms is key to improving our understanding of environmental systems. In this context, Unmanned Aerial Systems (UAS) have considerable potential to radically improve environmental monitoring. UAS-mounted sensors offer an extraordinary opportunity to bridge the existing gap between field observations and traditional air-and space-borne remote sensing, by providing high spatial detail over relatively large areas in a cost-effective way and an entirely new capacity for enhanced temporal retrieval. As well as showcasing recent advances in the field, there is also a need to identify and understand the potential limitations of UAS technology. For these platforms to reach their monitoring potential, a wide spectrum of unresolved issues and application-specific challenges require focused community attention. Indeed, to leverage the full potential of UAS-based approaches, sensing technologies, measurement protocols, postprocessing techniques, retrieval algorithms, and evaluation techniques need to be harmonized. The aim of this paper is to provide an overview of the existing research and applications of UAS in natural and agricultural ecosystem monitoring in order to identify future directions, applications, developments, and challenges.The present work has been funded by the COST Action CA16219 "HARMONIOUS-Harmonization of UAS techniques for agricultural and natural ecosystems monitoring". B. Toth acknowledges financial support by the Hungarian National Research, Development and Innovation Office (NRDI) under grant KH124765. J. Millerovd was supported by projects GA17-13998S and RVO67985939. Isabel and Jodo de Lima were supported by project HIRT (PTDC/ECM-HID/4259/2014-POCI-0145-FEDER016668).Manfreda, S.; Mccabe, MF.; Miller, PE.; Lucas, R.; Pajuelo Madrigal, V.; Mallinis, G.; Ben Dor, E.... (2018). On the Use of Unmanned Aerial Systems for Environmental Monitoring. Remote Sensing. 10(4):1-28. https://doi.org/10.3390/rs10040641S12810

Genetic analyses of aplastic anemia and idiopathic pulmonary fibrosis patients with short telomeres, possible implication of DNA-repair genes

Background: Telomeres are nucleoprotein structures present at the terminal region of the chromosomes. Mutations in genes coding for proteins involved in telomere maintenance are causative of a number of disorders known as telomeropathies. The genetic origin of these diseases is heterogeneous and has not been determined for a significant proportion of patients. Methods: This article describes the genetic characterization of a cohort of patients. Telomere length was determined by Southern blot and quantitative PCR. Nucleotide variants were analyzed either by high-resolution melting analysis and Sanger sequencing of selected exons or by massive sequencing of a panel of genes. Results: Forty-seven patients with telomere length below the 10% of normal population, affected with three telomeropathies: dyskeratosis congenita (4), aplastic anemia (22) or pulmonary fibrosis (21) were analyzed. Eighteen of these patients presented known pathogenic or novel possibly pathogenic variants in the telomere-related genes TERT, TERC, RTEL1, CTC1 and ACD. In addition, the analyses of a panel of 188 genes related to haematological disorders indicated that a relevant proportion of the patients (up to 35%) presented rare variants in genes related to DNA repair or in genes coding for proteins involved in the resolution of complex DNA structures, that participate in telomere replication. Mutations in some of these genes are causative of several syndromes previously associated to telomere shortening

Investigating the behavior of a small Mediterranean catchment using three different hydrological models as hypotheses

[EN] Mediterranean catchments are characterized by strong nonlinearities in their hydrological behaviour. Properly simulating those nonlinearities still represents a great challenge and, at the same time, an important issue in order to improve our knowledge of their hydrological behaviour. The main aim of this work is find out diverse modelling approaches to reproduce the observed nonlinear hydrological behaviour in a small Mediterranean catchment, Can Vila (Vallcebre, NE Spain). To this end, three hydrological models were considered: two lumped models called LU3 and LU4 of increasing complexity, and a distributed model called TETIS. The structures of these different models were used as hypotheses, which could explain and reproduce the observed nonlinear behaviour at the outlet. Four analyses were carried out: (i) goodness-of-fit criteria analysis, (ii) residual errors analysis, (iii) sensitivity analysis and (iv) multicriteria analysis based on the concept of Pareto Optimal. These analyses showed the higher capability and robustness of the distributed model to reproduce the observed complex hydrological behaviour in this catchment.This study was funded by the Spanish projects ECOTETIS (CGL2011-28776-C02-C01), PROBASE (CGL2006-11619/HID), RespHiMed (CGL2010-18374), PAID-06-12 (UPPTE/2012/139) and MONTES (CSD2008-00040). Research at Vallcebre catchments is also supported by an agreement between CSIC and Spanish Environment Ministry. J. Latron was the beneficiary of a research contract funded by Spanish Science Ministry and C. Medici by the Regional Government of Valencia. The authors are indebted to the other members of the Surface Hydrology and Erosion Research Group at IDAEA and CSIC for providing the necessary data and assistance.Ruiz Perez, G.; Medici, C.; Latron, J.; Llorens, P.; Gallart, F.; Francés, F. (2016). Investigating the behavior of a small Mediterranean catchment using three different hydrological models as hypotheses. 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Table1_New pathogenic variant in DLX5: New clues for a clinical spectrum from split-hand-foot malformation to fibular aplasia, tibial campomelia and oligosyndactyly.DOCX

Introduction: FATCO (Fibular Aplasia, Tibial Campomelia and Oligosyndactyly) is a very infrequent skeletal dysplasia classified within the limb hypoplasia-reduction defects group whose genetic cause has not yet been identified. The advent of next-generation sequencing is enabling the diagnosis of diseases with no previously known genetic cause.Methods: We performed a thorough autopsy on a fetus whose pregnancy was legally terminated due to severe malformations detected by ultrasound. A trio exome was run to identify the genetic cause and risk of recurrence. Previous literature of similar cases was systematically searched.Results: Anatomopathological analyses revealed complete fibular aplasia, shortened and campomelic tibia, absent ankle joint, club right foot and a split foot malformation, leading to the diagnosis of FATCO. Exome sequencing showed that the female fetus carried a de novo nonsense variant in DLX5. The literature search permitted the collection of information on 43 patients with FATCO, the majority of whom were males diagnosed postnatally. In most cases, lower limbs were affected exclusively, but in 39.5% of cases the upper limbs were also affected.Conclusion: The pathologies associated with DLX5 variants encompass a wide spectrum of manifestations ranging from abnormalities exclusively in the hands and feet to long bones such as the tibia and fibula.</p