Examining Preservation Methods for Long-Term Fecal Matter Storage

Fecal Matter Transplants (FMT) are often used as a last resort treatment for patients who have a C. diff infection. Clostridium difficile (C. diff) is a common hospital-acquired pathogen which replaces healthy bacteria in the GI tract and disrupts their normal function. Due to antibiotic overuse, C. diff often resurfaces after antibiotic treatment. In order to more effectively treat C. diff and other bacterial diseases or infections, other methods of treatment should be utilized. FMT’s use stool samples from healthy individuals to reestablish the microbiota in the gut after an infection occurs. Because of U.S. Food and Drug Administration regulations and social stigma, antibiotic treatments are the preferred method to treat C. diff. In order to reduce the social stigma surrounding FMT’s, research of further methods of fecal matter preservation could lead to a more personalized stool banking process. Utilization of personally banked stool samples could more effectively reintroduce naturally growing bacteria in the gut and decrease the chance of illness after treatment. Previous data verifies the preservation of DNA in samples that have been stored. However, the viability of the cells was unknown. The focus of the conducted research this summer was to test the viability of cells using stool samples donated by dogs. Samples were stored at -80oC in four different conditions for three weeks to determine the best method for cell preservation. Our goal was to identify the method that best retains cell function. To measure the metabolic activity of living cells after storage, BioLOG plates were used. Analysis of the data collected from the BioLOG plates will help to determine cell viability and assist in supporting further preservation methods in the future

Lesson Plan, Social Studies, 2nd Grade and 3rd Grade

TEKS (Texas Essential Knowledge and Skills): History(C) explain how people and events have influenced local community history. (5) Geography. The student uses simple geographic tools such as maps and globes. The student is expected to: (A) interpret information on maps and globes using basic map elements such as title, orientation (north, south, east, west), and legend/map keys; and (B) create maps to show places and routes within the home, school, and community. (6) Geography. The student understands the locations and characteristics of places and regions in the community, state, and nation. The student is expected to: (A) identify major landforms and bodies of water, including each of the continents and each of the oceans, on maps and globes; (B) locate places of significance, including the local community, Texas, the state capital, the U.S. capital, major cities in Texas, the coast of Texas, Canada, Mexico, and the United States on maps and globes; and (C) examine information from various sources about places and regions. Lesson objective(s): TLW learn the local history of Port Isabel, TX. TLW learn the history of The Charles Champion Building. TLW be able to locate Port Isabel, TX and the Gulf of Mexico on a map Differentiation strategies to meet diverse learner needs: 1. Students can differentiate their own presentation. Teacher will offer choices. 2. Buddy system-students can work on project independently or with a buddy 3. Presenting ideas through both auditory and visual mean

Analytical ray transfer matrix for the crystalline lens

We present the formulation of a paraxial ray transfer or ABCD matrix for onion-type GRIN lenses. In GRIN lenses, each iso-indicial surface (IIS) can be considered a refracting optical surface. If each IIS is a shell or layer, the ABCD matrix of a GRIN lens is computed by multiplying a typically high number of translation and refraction matrices corresponding to the K layers inside the lens. Using a differential approximation for the layer thickness, this matrix product becomes a sum. The elements A, B, C, and D of the approximated GRIN ray transfer matrix can be calculated by integrating the elements of a single-layer matrix. This ABCD matrix differs from a homogeneous lens matrix in only one integration term in element C, corresponding to the GRIN contribution to the lens power. Thus the total GRIN lens power is the sum of the homogeneous lens power and the GRIN contribution, which offers a compact and simple expression for the ABDC matrix. We then apply this formulation to the crystalline lens and implement both numerical and analytical integration procedures to obtain the GRIN lens power. The analytical approximation provides an accurate solution in terms of Gaussian hypergeometric functions. Last, we compare our numerical and analytical procedures with published ABCD matrix methods in the literature, and analyze the effect of the iso-indicial surface’s conic constant (Q) and inner curvature gradient (G) on the lens power for different lens models

Intracapsular accommodation mechanism in terms of lens curvature gradient

The intracapsular accommodation mechanism (IAM) may be understood as an increase in the lens equivalent refractive index as the eye accommodates. Our goal was to evaluate the existence of an IAM by analysing observed changes in the inner curvature gradient of the lens. To this end, we fitted a gradient index and curvature lens model to published experimental data on external and nucleus geometry changes during accommodation. For each case analysed, we computed the refractive power and equivalent index for each accommodative state using a ray transfer matrix. All data sets showed an increase in the effective refractive index, indicating a positive IAM, which was stronger for older lenses. These results suggest a strong dependence of the lens equivalent refractive index on the inner curvature gradient

Drylines in Argentina: Synoptic Climatology and Processes Leading to Their Genesis

Drylines have been identified as relevant synoptic-scale phenomena that frequently occur in several regions around the world. Despite previous works and the experience of local forecasters that recognizes the occurrence of drylines in Argentina and suggests its possible association with convection initiation, knowledge about the mechanisms leading to the genesis of these features is poor. This paper presents the first synoptic climatology of these drylines as well as a first approach to the understanding of the processes leading to their formation. The climatology is based on an automated algorithm for dryline identification applied to reanalysis data. We found that drylines are more frequent between the northern Patagonia plateau and the central Argentinean plains. A composite analysis is performed to analyze the processes leading to the formation of synoptic-scale drylines within this region. It was found that these drylines form in the confluence between a warm and moist air mass driven by a northwesterly flow and drier air flowing east over the northern Patagonia plateau. The dry air originates on top of the Pacific maritime boundary layer and experiences lee subsidence after crossing the Andes range creating an area of dry and warm air that is advected to the east by the westerly synoptic-scale flow, and transported downward during the day due to strong boundary layer turbulence. At the same time, surface heating over the plateau leads to substantial warming of the originally colder dry air behind the dryline, thus reversing the horizontal temperature gradient across the dryline.Fil: Bechis, Hernán. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones del Mar y la Atmósfera; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones del Mar y la Atmósfera. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones del Mar y la Atmósfera; ArgentinaFil: Salio, Paola Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones del Mar y la Atmósfera. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones del Mar y la Atmósfera; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones del Mar y la Atmósfera; ArgentinaFil: Ruiz, Juan Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones del Mar y la Atmósfera. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones del Mar y la Atmósfera; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones del Mar y la Atmósfera; Argentin

Preparation of quantum dots hydrogel nanocomposites with improved cytotoxicity

Nanocomposites are materials with unique properties and a wide range of applications. The combination of different nanostructures with traditional materials gives a variety of possibilities that should be analyzed. Especially, functional fluorescent semiconductor quantum dots (QDs) embedded in polymeric matrices have shown promising fluorescence and biocompatibility properties. These hybrid materials can be used in medical applications such as biodiagnostic and bioimaging. In this study, two hydrogels, one of polyethylene glycol diacrylate (PEGDA) and other of polyacrylamide (PAAm), were prepared with quantum dots of CdTe (4 nm of diameter) and characterized. The aim of this research was to analyze the optical properties of the nanocomposites and their cell viability. QDs nanocomposites were fabricated by a free radical polymerization process. The optical studies showed that the nanocomposites have well defined properties of fluorescence. To study the biocompatibility of the nanocomposites, metastatic B16f10 cell line were used and MTT assay was performed. The nanocomposites had a significant improved cell viability compared with QDs solutions

Incarcerated Hispanic females with disabilities : perceived barriers returning to public schools

While there is a significant amount of research centered around minority youth and their barriers toward transition back into a traditional school setting, the research paints very broad pictures of the youth who find themselves in these unique circumstances. There is a lack in research that deals with specific subpopulations of the groups targeted by the existing research. While one article may center around minority youth, much of the research would be conducted with males rather than females. While this research is insightful, it does not produce a complete picture. The research presented herein was conducted at a residential treatment facility over the course of six months, and focused on a sample of six Hispanic girls, ages 13-14, who have been identified as needing special education services. This study is meant to be seen through the lens of self-determination theory, which provides insight into the perspective and viewpoint of each participant. When searching for the causes of student and school failure, deficit thinking can be present within the view of those involved. Therefore, the effects of deficit thinking is taken into consideration within this study. The data was collected via interviews and surveys in which each girl participated, and an analysis of the documents and records located in each girl’s educational file. Since the nature of qualitative research calls for the emergence of themes that arise under each research question, the results of the research are presented through the prevailing themes of each of the research questions. This study found, the girls are aware of the challenges that can arise from returning to a traditional school setting, yet recognize what they and teachers can do to ameliorate potentially negative situations. Likewise, the research found that teacher relationships and teacher interactions with students are vital to transition. The study also found that the girls do have goals for the future and the prospect of future peer interactions present a problem as well. While this study provides insight into an overlooked population, it also tells the story of six strong young women who otherwise may have gone unseen.Special Educatio

Frecuencia del Consumo de Alimentos Ultra Procesados en Adolescentes de la I.E. María Reiche Newman. Ate - Vitarte

El consumo de alimentos ultra procesados está aumentando de manera vertiginosa en la población escolar, poniendo en riesgo a si la salud y el futuro de los más jóvenes. El objetivo del estudio es determinar la prevalencia del consumo de alimentos ultra procesados en adolescentes de la I.E. María Reiche Newman. Ate-Vitarte. Es un estudio tipo descriptivo, de diseño no experimental de corte transversal que incluyó la participación de 99 adolescentes de ambos sexos entre 12 a 19 años estudiantes del nivel secundario. Se evaluó el consumo de alimentos ultra procesados y se categorizó en alto consumo igual o > 5 veces/semana y bajo consumo <5 veces/semana. La prevalencia de consumo alto de alimentos ultra procesados fue de 26.3% y el 73.7% fue bajo consumo. El consumo de alimentos ultra procesados no fue altamente prevalente en este grupo de adolescentes, sin embargo, se necesitan más estudios en este campo y establecer consensos sobre ingesta, esta prevalencia no deja de ser importante debido al acentuado problema del sobrepeso y obesidad en el PerúTrabajo de investigaciónLIMAEscuela Profesional de Nutrición HumanaNutrición vegetariana y promoción de la salu

Miedo escénico y habilidades socioemocionales lingüísticas

El artículo tuvo como objetivo analizar estudiossobre el miedo escénico y laincidencia sobre eldesarrollo delashabilidades socioemocionales lingüísticas delos estudiantes.Como metodología,se utilizó una revisión sistemática de la literatura,considerando unnivel descriptivo. Para ello, se examinaronartículos científicos publicados en las bases de datos de Scopus, Dialnet, ERIHPLus y Latindex. Los resultados develaron que el país con mayor cantidad de publicaciones es España, seguido por Ecuador; la base de datos con mayor cantidad de publicaciones fue Latindex mientras que Scopus ocupó el tercer lugar; el año donde se realizó el mayor porcentaje de publicaciones fue el 2022yel nivel educativo preferido para investigar fue el superior.Como conclusión, se precisóla necesidad de ampliar el campo de estudio sobre esta temática debido al impacto que tiene especialmenteen los adolescentes