De Novo Sporophyte Transcriptome Assembly and Functional Annotation in the Endangered Fern Species Vandenboschia speciosa (Willd.) G. Kunkel

We sequenced the sporophyte transcriptome of Killarney fern (Vandenboschia speciosa (Willd.) G. Kunkel). In addition to being a rare endangered Macaronesian-European endemism, this species has a huge genome (10.52 Gb) as well as particular biological features and extreme ecological requirements. These characteristics, together with the systematic position of ferns among vascular plants, make it of high interest for evolutionary, conservation and functional genomics studies. The transcriptome was constructed de novo and contained 36,430 transcripts, of which 17,706 had valid BLAST hits. A total of 19,539 transcripts showed at least one of the 7362 GO terms assigned to the transcriptome, whereas 6547 transcripts showed at least one of the 1359 KEGG assigned terms. A prospective analysis of functional annotation results provided relevant insights on genes involved in important functions such as growth and development as well as physiological adaptations. In this context, a catalogue of genes involved in the genetic control of plant development, during the vegetative to reproductive transition, in stress response as well as genes coding for transcription factors is given. Altogether, this study provides a first step towards understanding the gene expression of a significant fern species and the in silico functional and comparative analyses reported here provide important data and insights for further comparative evolutionary studies in ferns and land plants in general.Spanish Ministerio de Economía y Competitividad and FEDER founds, grant: CGL2010-14856 (subprograma BOS)

Proteins reversibly oxidized in cysteines in endothellial cells in response to acute hypoxia

Comunicaciones a congreso

Proteómica redox diferencial: identificación de proteínas oxidadas reversiblemente en respuesta a hipoxia

Comunicaciones a congreso

Identification and Characterization of TALE Homeobox Genes in the Endangered Fern Vandenboschia speciosa

We report and discuss the results of a quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis of the expression patterns of seven three amino acid loop extension (TALE) homeobox genes (four KNOTTED-like homeobox (KNOX) and three BEL1-like homeobox (BELL) genes) identified after next generation sequencing (NGS) and assembly of the sporophyte and gametophyte transcriptomes of the endangered fern species Vandenboschia speciosa. Among the four KNOX genes, two belonged to the KNOX1 class and the other two belonged to the KNOX2 class. Analysis of the deduced amino acid sequences supported the typical domain structure of both types of TALE proteins, and the homology to TALE proteins of mosses, lycophytes, and seed plant species. The expression analyses demonstrate that these homeodomain proteins appear to have a key role in the establishment and development of the gametophyte and sporophyte phases of V. speciosa lifecycle, as well as in the control of the transition between both phases. Vandenboschia speciosa VsKNAT3 (a KNOX2 class protein) as well as VsBELL4 and VsBELL10 proteins have higher expression levels during the sporophyte program. On the contrary, one V. speciosa KNOX1 protein (VsKNAT6) and one KNOX2 protein (VsKNAT4) seem important during the development of the gametophyte phase. TALE homeobox genes might be among the key regulators in the gametophyte-to-sporophyte developmental transition in regular populations that show alternation of generations, since some of the genes analyzed here (VsKNAT3, VsKNAT6, VsBELL4, and VsBELL6) are upregulated in a non-alternating population in which only independent gametophytes are found (they grow by vegetative reproduction outside of the range of sporophyte distribution). Thus, these four genes might trigger the vegetative propagation of the gametophyte and the repression of the sexual development in populations composed of independent gametophytes. This study represents a comprehensive identification and characterization of TALE homeobox genes in V. speciosa, and gives novel insights about the role of these genes in fern development.This research has been financed by the Spanish Ministerio de Economía y Competitividad and FEDER founds, grant: CGL2010-14856 (subprograma BOS).M. Bakkali wishes to thank the Spanish Ministerio de Economía y Competitividad for granting him with a Ramón y Cajal Fellowship, the BFU2010-16438 grant, and an FPI studentship to R. Martín-Blázquez

Askin tumor: Case report and literature review

Askin tumor is an uncommon malignant neoplasm of a neuroectodermic origin that arises from the soft tissues of the thoracopulmonary wall. Defined histologically by Askin and Rosai in 1979 as a malignant small round cell tumor. It is described within a group of malignant neoplasms with an aggressive behavior. The lack of clinical guides that establish a standardized management contributes to its poor prognosis and short overall survival. Once a primitive neuroectodermal tumor has been diagnosed, treatment will consist of a multimodal management

Asimilación

Treballs de l'alumnat del Grau de Comunicació Audiovisual, Facultat de Biblioteconomia i Documentació, Universitat de Barcelona, [Metanarratives- Grup I]. Curs: 2016-2017, Tutor: Josep Rovira.Director: Rubén Cantón Alcalde; Aj. Direcció: Anaïs Tania Castaño Ramos; Productor: Juan Cristóbal Bravo Oller; Aj. Producció: Àlex Ensenyat Rubí; Guionistes: Juan Cristóbal Bravo Oller, Rubén Cantón Alcalde, Àlex Ensenyat Rubí, Lídia Hernández Cruz; Directora de fotografia: Lídia Hernández Cruz; Càmera: Rubén Cantón Alcalde; Aj. Càmera: Anaïs Tania Castaño Ramos, Lídia Hernández Cruz; Il·luminadora: Marta Coca Lozano; Direcció artística: Víctor Villaverde Ruiz; Direcció de so: Àlex Ensenyat Rubí; Muntatge: Rubén Cantón Alcalde, Marta Coca Lozano, Lídia Hernández Cruz; Postproducció: Marta Coca Lozano; Equip artístic: Víctor Villaverde Ruiz, Lídia Hernández Cruz.Una persona sense identitat entra en una sala. Allà, comença a interactuar amb objectes, convertint-se sempre en l'autor d'aquests

Proteínas de choque térmico durante la isquemia cerebral transitoria: ¿Un posible blanco terapéutico?

La enfermedad vascular cerebral (evc) es una de las causas de morbimortalidad más frecuentes en nuestro medio. Las proteínas de choque térmico son chaperonas moleculares que responden al estrés inducido por diversas lesiones. Ciertos estudios experimentales han descrito su papel en la respuesta adaptativa a la isquemia cerebral, caracterizando algunos de sus efectos moduladores de la respuesta inflamatoria y del ciclo celular. Presentamos un trabajo de revisión sistemática de la literatura acerca de los hallazgos experimentales de los modelos de lesión por isquemia-reperfusión y deprivación de oxígeno-glucosa, asociados con la función de las proteínas de choque térmico en células nerviosas. Se realizó una búsqueda avanzada de artículos relevantes o recientes sobre este tema, en bases de datos especializadas (PubMed, Google Scholar, Scielo, MEDLINE y Cochrane Library). Los estudios revisados demuestran que estas chaperonas moleculares modulan la respuesta a la isquemia cerebral transitoria, controlando mecanismos adaptativos que favorecen la tolerancia del cerebro a la isquemia y la inhibición de la apoptosis en las células nerviosas. Estos resultados experimentales permiten entrever una posible utilidad terapéutica de estos mecanismos moleculares, considerando a las proteínas de choque térmico como prometedores blancos terapéuticos para el tratamiento de la enfermedad vascular cerebral y otras patologías

Correlation Analysis of People Attitude and the Development of Chemical Processes in the Human Body

This chemical science investigation was made to efficiently understand the way in which ionic substances operate in the human body and its correlation that they can have with the attitude of people, whose evaluation was carried out according to the analysis of three actions developed in the human body, such as digestion factors, family, work and social relationships (emotional actions) and the health of 10 people surveyed in the city of Tijuana, Baja California. This evaluation had a main hypothesis at the beginning of the investigation, indicating that, of the three factors mentioned, the one that links emotions had the greatest effect on the behavior of the people evaluated, with the aspect of family and work relationships being of primary importance; followed by the health factor and finally the digestion factor. This scientific study included an analysis of three principal aspects, being first the daily food as a nutrition action, followed a chemical analysis of ionic substances of the human body of the 10 persons evaluated. In addition, the third aspect evaluated was the emotional state of the people and a clinic with the urine analysis were considered to indicate the pH, temperature and oxygen level of each person, with which the main chemical elements of the ionic substances that can circulate through the human body were obtained, as part of the food intake. and drinks, emotional factor and digestion. This scientific study was made from March to May of 2023

On the Tunability of Toxicity for Viologen‐Derivatives as Anolyte for Neutral Aqueous Organic Redox Flow Batteries

Viologen-derivatives are the most widely used redox organic molecules for neutral pH negative electrolyte of redox flow batteries. However, the long-established toxicity of the herbicide methyl-viologen raises concern for deployment of viologen-derivatives at large scale in flow batteries. Herein, we demonstrate the radically different cytotoxicity and toxicology of a series of viologen-derivatives in in vitro assays using model organisms representative of human and environmental exposure, namely human lung carcinoma epithelial cell line (A549) and the yeast Saccharomyces cerevisiae. The results show that safe viologen derivatives can be molecularly engineered, representing a promising family of negolyte materials for neutral redox flow batteries.The authors acknowledge financial support by the Spanish Government (Ministerio de Ciencia e Innovacion, Grants PID2021-124974OB-C22 and PID2020-115789GB-C21) and Ramon y Cajal award (RYC2018-026086-I) as well as the MeBattery project. MeBattery has received funding from the European Innovation Council of the European Union under Grant Agreement no. 101046742. This work was supported by the Regional Government of Castilla y Leon (Junta de Castilla y Leon) and by the Ministry of Science and Innovation MICIN and the European Union NextGenerationEU/PRTR. We also gratefully acknowledge Junta de Castilla y Leon (BU049P20) and FEDER for financial support