C9ORF72 patient-derived endothelial cells drive blood-brain barrier disruption and contribute to neurotoxicity

The blood-brain barrier (BBB) serves as a highly intricate and dynamic interface connecting the brain and the bloodstream, playing a vital role in maintaining brain homeostasis. BBB dysfunction has been associated with multiple neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS); however, the role of the BBB in neurodegeneration is understudied. We developed an ALS patient-derived model of the BBB by using cells derived from 5 patient donors carrying C9ORF72 mutations. Brain microvascular endothelial-like cells (BMEC-like cells) derived from C9ORF72-ALS patients showed altered gene expression, compromised barrier integrity, and increased P-glycoprotein transporter activity. In addition, mitochondrial metabolic tests demonstrated that C9ORF72-ALS BMECs display a significant decrease in basal glycolysis accompanied by increased basal and ATP-linked respiration. Moreover, our study reveals that C9-ALS derived astrocytes can further affect BMECs function and affect the expression of the glucose transporter Glut-1. Finally, C9ORF72 patient-derived BMECs form leaky barriers through a cell-autonomous mechanism and have neurotoxic properties towards motor neurons

Proton elastic scattering on light neutron-rich nuclei

Proton-nucleus elastic scattering angular distributions have been measured for 6He, 7Li, 10Be, 11Be secondary beams. These data, together with other elastic scattering data for proton on unstable nuclei, have been analyzed using phenomenological and microscopic optical model approaches. To reproduce the experimental angular distributions for unstable nuclei, it was necessary to renormalize either the real or imaginary part of the optical potential. The influence of the break-up channels on the results is discussed

Dust Emission in Galaxies at Millimeter Wavelengths: Cooling of star forming regions in NGC6946

International audienceInterstellar dust plays an important role in the formation of molecular gas and the heating and cooling of the interstellar medium. The spatial distribution of the mm-wavelength dust emission from galaxies is largely unexplored. The NIKA2 Guaranteed Time Project IMEGIN (Interpreting the Millimeter Emission of Galaxies with IRAM and NIKA2) has recently mapped the mm emission in the grand design spiral galaxy NGC6946. By subtracting the contributions from the free-free, synchrotron, and CO line emission, we map the distribution of the pure dust emission at 1:15mm and 2mm. Separating the arm/interarm regions, we find a dominant 2mm emission from interarms indicating the significant role of the general interstellar radiation field in heating the cold dust. Finally, we present maps of the dust mass, temperature, and emissivity index using the Bayesian MCMC modeling of the spectral energy distribution in NGC6946

IAS/CEA Evolution of Dust in Nearby Galaxies (ICED): the spatially-resolved dust properties of NGC4254

International audienceWe present the first preliminary results of the project \textit{ICED}, focusing on the face-on galaxy NGC4254. We use the millimetre maps observed with NIKA2 at IRAM-30m, as part of the IMEGIN Guaranteed Time Large Program, and of a wide collection of ancillary data (multi-wavelength photometry and gas phase spectral lines) that are publicly available. We derive the global and local properties of interstellar dust grains through infrared-to-radio spectral energy distribution fitting, using the hierarchical Bayesian code HerBIE, which includes the grain properties of the state-of-the-art dust model, THEMIS. Our method allows us to get the following dust parameters: dust mass, average interstellar radiation field, and fraction of small grains. Also, it is effective in retrieving the intrinsic correlations between dust parameters and interstellar medium properties. We find an evident anti-correlation between the interstellar radiation field and the fraction of small grains in the centre of NGC4254, meaning that, at strong radiation field intensities, very small amorphous carbon grains are efficiently destroyed by the ultra-violet photons coming from newly formed stars, through photo-desorption and sublimation. We observe a flattening of the anti-correlation at larger radial distances, which may be driven by the steep metallicity gradient measured in NGC4254

IAS/CEA Evolution of Dust in Nearby Galaxies (ICED): The spatially-resolved dust properties of NGC4254

We present the first preliminary results of the project ICED, focusing on the face-on galaxy NGC4254. We use the millimetre maps observed with NIKA2 at lRAM-30m. as part of the IMEGIN Guaranteed Time Large Program. and of a wide collection of ancillary data (multi-wavelength photometry and gas phase spectral lines) that are publicly available. We derive the global and local properties of interstellar dust grains through infrared-to-radio spectral energy distribution fitting, using the hierarchical Bayesian code HerBIE. which includes the grain properties of the state-of-the-art dust model. THEMIS. Our method allows us to get the following dust parameters: dust mass, average interstellar radiation field, and fraction of small grains. Also, it is effective in retrieving the intrinsic correlations between dust parameters and interstellar medium properties. We find an evident anti-correlation between the interstellar radiation field and the fraction of small grains in the centre of NGC4254. meaning that, at strong radiation field intensities, very small amorphous carbon grains are efficiently destroyed by the ultra-violet photons coming from newly formed stars, through photo-desorption and sublimation. We observe a flattening of the anti-correlation at larger radial distances, which may be driven by the steep metallicity gradient measured in NGC4254

Constraining Millimeter Dust Emission in Nearby Galaxies with NIKA2: the case of NGC2146 and NGC2976

International audienceThis study presents the first millimeter continuum mapping observations of two nearby galaxies, the starburst spiral galaxy NGC2146 and the dwarf galaxy NGC2976, at 1.15 mm and 2 mm using the NIKA2 camera on the IRAM 30m telescope, as part of the Guaranteed Time Large Project IMEGIN. These observations provide robust resolved information about the physical properties of dust in nearby galaxies by constraining their FIR-radio SED in the millimeter domain. After subtracting the contribution from the CO line emission, the SEDs are modeled spatially using a Bayesian approach. Maps of dust mass surface density, temperature, emissivity index, and thermal radio component of the galaxies are presented, allowing for a study of the relations between the dust properties and star formation activity (using observations at 24$\mu$m as a tracer). We report that dust temperature is correlated with star formation rate in both galaxies. The effect of star formation activity on dust temperature is stronger in NGC2976, an indication of the thinner interstellar medium of dwarf galaxies. Moreover, an anti-correlation trend is reported between the dust emissivity index and temperature in both galaxies