A New Static Analytic Apparatus for Complete Determination of Phase Equilibrium Data from Sub-atmospheric Pressures up to 10 bar

International audienceA new static-analytic apparatus equipped two ROLSI TM capillary samplers was presented.The proposed improvements allow ROLSI TM to perform in-situ sampling even at P < P carrier gas. The apparatus has shown comparable performances to existing methods, while showing some advantages. Two binary systems of interest were investigated. New systems are expected to be investigated for various applications in chemical engineering. The liquid sampling mechanism is expected to be used for liquid-liquid equilibrium measurement

Effects of self-generated electric and magnetic fields in laser-generated fast electron propagation in solid materials: Electric inhibition and beam pinching

We present some experimental results which demonstrate the presence of electric inhibition in the propagation of relativistic electrons generated by intense laser pulses, depending on target conductivity. The use of transparent targets and shadowgraphic techniques has made it possible to evidence electron jets moving at the speed of light, an indication of the presence of self-generated strong magnetic fields

Identification of a novel BET bromodomain inhibitor-sensitive, gene regulatory circuit that controls Rituximab response and tumour growth in aggressive lymphoid cancers.: CYCLON-induced Rituximab resistance

International audienceImmuno-chemotherapy elicit high response rates in B-cell non-Hodgkin lymphoma but heterogeneity in response duration is observed, with some patients achieving cure and others showing refractory disease or relapse. Using a transcriptome-powered targeted proteomics screen, we discovered a gene regulatory circuit involving the nuclear factor CYCLON which characterizes aggressive disease and resistance to the anti-CD20 monoclonal antibody, Rituximab, in high-risk B-cell lymphoma. CYCLON knockdown was found to inhibit the aggressivity of MYC-overexpressing tumours in mice and to modulate gene expression programs of biological relevance to lymphoma. Furthermore, CYCLON knockdown increased the sensitivity of human lymphoma B cells to Rituximab in vitro and in vivo. Strikingly, this effect could be mimicked by in vitro treatment of lymphoma B cells with a small molecule inhibitor for BET bromodomain proteins (JQ1). In summary, this work has identified CYCLON as a new MYC cooperating factor that autonomously drives aggressive tumour growth and Rituximab resistance in lymphoma. This resistance mechanism is amenable to next-generation epigenetic therapy by BET bromodomain inhibition, thereby providing a new combination therapy rationale for high-risk lymphoma

Fast electron transport and heating in solid-density matter

Two experiments have been performed to investigate heating by high-intensity laser-generated electrons, in the context of studies of the fast ignitor approach to inertial confinement fusion (ICF). A new spectrometer and layered targets have been used to detect Kα emission from aluminum heated by a fast electron beam. Results show that a temperature of about 40 eV is reached in solid density aluminum up to a depth of about 100 μm

The Testis-Specific Factor CTCFL Cooperates with the Protein Methyltransferase PRMT7 in H19 Imprinting Control Region Methylation

Expression of imprinted genes is restricted to a single parental allele as a result of epigenetic regulation—DNA methylation and histone modifications. Igf2/H19 is a reciprocally imprinted locus exhibiting paternal Igf2 and maternal H19 expression. Their expression is regulated by a paternally methylated imprinting control region (ICR) located between the two genes. Although the de novo DNA methyltransferases have been shown to be necessary for the establishment of ICR methylation, the mechanism by which they are targeted to the region remains unknown. We demonstrate that CTCFL/BORIS, a paralog of CTCF, is an ICR-binding protein expressed during embryonic male germ cell development, coinciding with the timing of ICR methylation. PRMT7, a protein arginine methyltransferase with which CTCFL interacts, is also expressed during embryonic testis development. Symmetrical dimethyl arginine 3 of histone H4, a modification catalyzed by PRMT7, accumulates in germ cells during this developmental period. This modified histone is also found enriched in both H19 ICR and Gtl2 differentially methylated region (DMR) chromatin of testis by chromatin immunoprecipitation (ChIP) analysis. In vitro studies demonstrate that CTCFL stimulates the histone-methyltransferase activity of PRMT7 via interactions with both histones and PRMT7. Finally, H19 ICR methylation is demonstrated by nuclear co-injection of expression vectors encoding CTCFL, PRMT7, and the de novo DNA methyltransferases, Dnmt3a, -b and -L, in Xenopus oocytes. These results suggest that CTCFL and PRMT7 may play a role in male germline imprinted gene methylation

Etude de l'instabilite Raman et de la generation d'electrons a hautes energies dans l'interaction laser-plasma

SIGLEINIST T 73396 / INIST-CNRS - Institut de l'Information Scientifique et TechniqueFRFranc

Application of a data minig based technique for the evaluation of transmission expansion plans

peer reviewedThis paper describes a methodology for the study of long-term network planning under uncertainties. In this approach the major external uncertainties during the planning horizon are modelled as macro-scenarios at different future time instants. The random nature of actual operating conditions is taken into account by using a probabilistic model of micro-scenarios based on past statistics. MonteCarlo simulations are used to generate and simulate a specified number of scenarios. Data mining techniques are then applied to the simulations results collected in a database, so as to extract information and to rank scenarios and network reinforcements according to different performance criteria. The paper describes the application of this approach on a real transmission planning problem faced by the Belgian transmission system operator

Utilisation de la spectrométrie de masse maldi-tof pour différencier un couple de souches isogéniques de candida glabrata sensible et résistante à la caspofungine

Candida glabrata est une levure émergente responsable d infections invasives. Elle est naturellement résistante aux azolés et de sensibilité diminuée à l amphotéricine B. La caspofungine constitue donc une alternative thérapeutique séduisante. Afin de déterminer le plus rapidement possible la sensibilité des levures aux antifongiques, nous avons évalué la spectrométrie de masse MALDI-TOF comme outil de détermination de la sensibilité de C. glabrata à la caspofungine. A partir d un couple de souches isogéniques, sensible et résistante, et après optimisation du protocole, nous avons pu mettre en évidence des modifications spectrométriques permettant la discrimination des souches selon leur susceptibilité après une incubation de 3 heures en présence de la drogue. Ces résultats prometteurs nécessitent des travaux complémentaires afin d être confirmés sur d autres souches et d être élargis à d autres espèces voire à d autres antifongiques.CHATENAY M.-PARIS 11-BU Pharma. (920192101) / SudocSudocFranceF

Contribution à l'étude des protéines de la thèque périnucléaire du spermatozoïde

La spermiogenèse, étape finale de la spermatogenèse, se caractérise par de nombreuses modifications morphologiques aboutissant à la transformation d'une spermatide ronde en spermatozoïde. La morphogenèse de la tête du spermatozoïde avec la mise en place de l'acrosome est un des exemples les plus importants. De nombreux travaux suggèrent l'implication de l'actine filamenteuse aidée par un cytosquelette périnucléaire pour placer et étaler l'acrosome autour du noyau. Les protéines qui composent ce cytosquelette périnucléaire (ou thèque périnucléaire) ne sont pas toutes identifiées ; parmi celles qui ont été caractérisées, beaucoup sont spécifiques de la lignée germinale mâle. L'objectif de notre travail a été de rechercher parmi les protéines du cytosquelette périnucléaire celles qui se lient à l'actine filamenteuse et de rechercher leur présence, avec celle de l'actine, au cours de la spermiogenèse.LILLE1-BU (590092102) / SudocSudocFranceF