202 research outputs found
Why Urban Parents Resist Involvement in Their Children’s Elementary Education
We examined the perceptions of teachers and parents about family involvement in urban schools. The study generated from several others that we have been conducting about teaching in high poverty, urban schools. Using focus groups, our purpose was to learn how we could better prepare teachers for urban schools. The data revealed that teachers are frustrated with a lack of parental involvement in literacy activities at home and at school. Parents, however, expressed distrust toward the local elementary school because they felt the faculty has been biased against African American and Latino children and their families. Consequently, the parents said they deliberately decided not to participate in school activities. Parents explained they would only work with teachers who respected and valued their children. Results of our study point to the importance of helping new teachers learn strategies for developing strong trusting relationships and effective communication strategies when working with urban families
Interaction between the tRNA-Binding and C-Terminal Domains of Yeast Gcn2 Regulates Kinase Activity In Vivo
Citation: Lageix, S., Zhang, J. W., Rothenburg, S., & Hinnebusch, A. G. (2015). Interaction between the tRNA-Binding and C-Terminal Domains of Yeast Gcn2 Regulates Kinase Activity In Vivo. Plos Genetics, 11(2), 28. doi:10.1371/journal.pgen.1004991The stress-activated protein kinase Gcn2 regulates protein synthesis by phosphorylation of translation initiation factor eIF2 alpha. Gcn2 is activated in amino acid-deprived cells by binding of uncharged tRNA to the regulatory domain related to histidyl-tRNA synthetase, but the molecular mechanism of activation is unclear. We used a genetic approach to identify a key regulatory surface in Gcn2 that is proximal to the predicted active site of the HisRS domain and likely remodeled by tRNA binding. Mutations leading to amino acid substitutions on this surface were identified that activate Gcn2 at low levels of tRNA binding (Gcd(-) phenotype), while other substitutions block kinase activation (Gcn(-) phenotype), in some cases without altering tRNA binding by Gcn2 in vitro. Remarkably, the Gcn(-) substitutions increase affinity of the HisRS domain for the C-terminal domain (CTD), previously implicated as a kinase autoinhibitory segment, in a manner dampened by HisRS domain Gcd(-) substitutions and by amino acid starvation in vivo. Moreover, tRNA specifically antagonizes HisRS/CTD association in vitro. These findings support a model wherein HisRS-CTD interaction facilitates the autoinhibitory function of the CTD in nonstarvation conditions, with tRNA binding eliciting kinase activation by weakening HisRS-CTD association with attendant disruption of the autoinhibitory KD-CTD interaction
Long Range Correlation in Granular Shear Flow II: Theoretical Implications
Numerical simulations are used to test the kinetic theory constitutive
relations of inertial granular shear flow. These predictions are shown to be
accurate in the dilute regime, where only binary collisions are relevant, but
underestimate the measured value in the dense regime, where force networks of
size are present. The discrepancy in the dense regime is due to
non-collisional forces that we measure directly in our simulations and arise
from elastic deformations of the force networks. We model the non-collisional
stress by summing over all paths that elastic waves travel through force
networks. This results in an analytical theory that successfully predicts the
stress tensor over the entire inertial regime without any adjustable
parameters
Stress-strain behavior and geometrical properties of packings of elongated particles
We present a numerical analysis of the effect of particle elongation on the
quasistatic behavior of sheared granular media by means of the Contact Dynamics
method. The particle shapes are rounded-cap rectangles characterized by their
elongation. The macroscopic and microstructural properties of several packings
subjected to biaxial compression are analyzed as a function of particle
elongation. We find that the shear strength is an increasing linear function of
elongation. Performing an additive decomposition of the stress tensor based on
a harmonic approximation of the angular dependence of branch vectors, contact
normals and forces, we show that the increasing mobilization of friction force
and the associated anisotropy are key effects of particle elongation. These
effects are correlated with partial nematic ordering of the particles which
tend to be oriented perpendicular to the major principal stress direction and
form side-to-side contacts. However, the force transmission is found to be
mainly guided by cap-to-side contacts, which represent the largest fraction of
contacts for the most elongated particles. Another interesting finding is that,
in contrast to shear strength, the solid fraction first increases with particle
elongation, but declines as the particles become more elongated. It is also
remarkable that the coordination number does not follow this trend so that the
packings of more elongated particles are looser but more strongly connected.Comment: Submited to Physical Review
Multiscale Analysis of the Stress State in a Granular Slope in Transition to Failure
By means of contact dynamics simulations, we analyze the stress state in a
granular bed slowly tilted towards its angle of repose. An increasingly large
number of grains are overloaded in the sense that they are found to carry a
stress ratio above the Coulomb yield threshold of the whole packing. Using this
property, we introduce a coarse-graining length scale at which all stress
ratios are below the packing yield threshold. We show that this length
increases with the slope angle and jumps to a length comparable to the depth of
the granular bed at an angle below the angle of repose. This transition
coincides with the onset of dilatation in the packing. We map this transition
into a percolation transition of the overloaded grains, and we argue that in
the presence of long-range correlations above the transition angle, the
granular slope is metastable.Comment: 11 pages, 14 Fig, submitted to PR
Force transmission in a packing of pentagonal particles
We perform a detailed analysis of the contact force network in a dense
confined packing of pentagonal particles simulated by means of the contact
dynamics method. The effect of particle shape is evidenced by comparing the
data from pentagon packing and from a packing with identical characteristics
except for the circular shape of the particles. A counterintuitive finding of
this work is that, under steady shearing, the pentagon packing develops a lower
structural anisotropy than the disk packing. We show that this weakness is
compensated by a higher force anisotropy, leading to enhanced shear strength of
the pentagon packing. We revisit "strong" and "weak" force networks in the
pentagon packing, but our simulation data provide also evidence for a large
class of "very weak" forces carried mainly by vertex-to-edge contacts. The
strong force chains are mostly composed of edge-to-edge contacts with a marked
zig-zag aspect and a decreasing exponential probability distribution as in a
disk packing
Solid behavior of anisotropic rigid frictionless bead assemblies
We investigate the structure and mechanical behavior of assemblies of
frictionless, nearly rigid equal-sized beads, in the quasistatic limit, by
numerical simulation. Three different loading paths are explored: triaxial
compression, triaxial extension and simple shear. Generalizing recent results
[1], we show that the material, despite rather strong finite sample size
effects, is able to sustain a finite deviator stress in the macroscopic limit,
along all three paths, without dilatancy. The shape of the yield surface is
adequately described by a Lade-Duncan (rather than Mohr-Coulomb) criterion.
While scalar state variables keep the same values as in isotropic systems,
fabric and force anisotropies are each characterized by one parameter and are
in one-to-one correspondence with principal stress ratio along all three
loading paths.The anisotropy of the pair correlation function extends to a
distance between bead surfaces on the order of 10% of the diameter. The tensor
of elastic moduli is shown to possess a nearly singular, uniaxial structure
related to stress anisotropy. Possible stress-strain relations in monotonic
loading paths are also discussed
Unilateral interactions in granular packings: A model for the anisotropy modulus
Unilateral interparticle interactions have an effect on the elastic response
of granular materials due to the opening and closing of contacts during
quasi-static shear deformations. A simplified model is presented, for which
constitutive relations can be derived. For biaxial deformations the elastic
behavior in this model involves three independent elastic moduli: bulk, shear,
and anisotropy modulus. The bulk and the shear modulus, when scaled by the
contact density, are independent of the deformation. However, the magnitude of
the anisotropy modulus is proportional to the ratio between shear and
volumetric strain. Sufficiently far from the jamming transition, when
corrections due to non-affine motion become weak, the theoretical predictions
are qualitatively in agreement with simulation results.Comment: 6 pages, 5 figure
Sensitivity of the stress response function to packing preparation
A granular assembly composed of a collection of identical grains may pack
under different microscopic configurations with microscopic features that are
sensitive to the preparation history. A given configuration may also change in
response to external actions such as compression, shearing etc. We show using a
mechanical response function method developed experimentally and numerically,
that the macroscopic stress profiles are strongly dependent on these
preparation procedures. These results were obtained for both two and three
dimensions. The method reveals that, under a given preparation history, the
macroscopic symmetries of the granular material is affected and in most cases
significant departures from isotropy should be observed. This suggests a new
path toward a non-intrusive test of granular material constitutive properties.Comment: 15 pages, 11 figures, some numerical data corrected, to appear in J.
Phys. Cond. Mat. special issue on Granular Materials (M. Nicodemi Editor
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