The Lantern Vol. 59, No. 2, Summer 1992

• Mr. Foley\u27s Toboggan • I Close the Door to the Bathroom • Insomniac Scribbles • And Then There Were Four • Goodbye, Ace • Silicone\u27s a Manmade Matter • The Nineteenth Hole • Upon Visiting Manor Care • Little Boys • Obsessed • Life • Shakespearean Shakedown • Violets and Morning Glories • Mr. Cope Takes His Secretary to Lunch • Winter Eyes • Triptych • These Hot, Humid Nights • The Car\u27s Place in His Heart • Saturday Night • The Windows of a Clean House • An Harmonious Thunk • Nomads • My Watch at Mass • Dave\u27s Fine Print • K.P. Duty • Serendipityhttps://digitalcommons.ursinus.edu/lantern/1141/thumbnail.jp

The Lantern Vol. 60, No. 2, Summer 1993

• Wake • Misconception • Cliche • Standard Oil • Lake Effect • Charlotte • Psychedelic Iridescent Infidelity • A Playground in Winter • Shooting Pool with Angels • The Blood Through Our Veins • Iced Coffee • Buzz Kill • Immortality • Cathodic Union • Crush • Mushrooms • Conversing • Eggplant • A Letter to the Civil Rights Movement • Still Sitting, Contemplating • Sensible Love • Monsters Under the Bed • Poison Rock • Waiting at the Dentist • Fate • Static • The Three C\u27s • As We Frolic • Nest • A Bottle of Wine and Patsy Cline • Bottoms of Pages, Backs of Bookshttps://digitalcommons.ursinus.edu/lantern/1143/thumbnail.jp

Broad chromosomal domains of histone modification patterns in C. elegans

Chromatin immunoprecipitation identifies specific interactions between genomic DNA and proteins, advancing our understanding of gene-level and chromosome-level regulation. Based on chromatin immunoprecipitation experiments using validated antibodies, we define the genome-wide distributions of 19 histone modifications, one histone variant, and eight chromatin-associated proteins in Caenorhabditis elegans embryos and L3 larvae. Cluster analysis identified five groups of chromatin marks with shared features: Two groups correlate with gene repression, two with gene activation, and one with the X chromosome. The X chromosome displays numerous unique properties, including enrichment of monomethylated H4K20 and H3K27, which correlate with the different repressive mechanisms that operate in somatic tissues and germ cells, respectively. The data also revealed striking differences in chromatin composition between the autosomes and between chromosome arms and centers. Chromosomes I and III are globally enriched for marks of active genes, consistent with containing more highly expressed genes, compared to chromosomes II, IV, and especially V. Consistent with the absence of cytological heterochromatin and the holocentric nature of C. elegans chromosomes, markers of heterochromatin such as H3K9 methylation are not concentrated at a single region on each chromosome. Instead, H3K9 methylation is enriched on chromosome arms, coincident with zones of elevated meiotic recombination. Active genes in chromosome arms and centers have very similar histone mark distributions, suggesting that active domains in the arms are interspersed with heterochromatin-like structure. These data, which confirm and extend previous studies, allow for in-depth analysis of the organization and deployment of the C. elegans genome during development

Representative sequencing: Unbiased sampling of solid tumor tissue

International audienceAlthough thousands of solid tumors have been sequenced to date, a fundamental under-sampling bias isinherent in current methodologies. This is caused by a tissue sample input of fixed dimensions (e.g., 6 mmbiopsy), which becomes grossly under-powered as tumor volume scales. Here, we demonstrate representative sequencing (Rep-Seq) as a new method to achieve unbiased tumor tissue sampling. Rep-Seq uses fixed residual tumor material, which is homogenized and subjected to next-generation sequencing. Analysis of intratumor tumor mutation burden (TMB) variability shows a high level of misclassification using current single-biopsy methods, with 20% of lung and 52% of bladder tumors having at least one biopsy with high TMB butlow clonal TMB overall. Misclassification rates by contrast are reduced to 2% (lung) and 4% (bladder) when a more representative sampling methodology is used. Rep-Seq offers an improved sampling protocol for tumor profiling, with significant potential for improved clinical utility and more accurate deconvolution of clonal structure