Validation of two pmx-luciferase reporter systems to study virus - host interaction.

Viral diseases represent an important threat in aquaculture. Therefore, the development of strategies and tools to understand fish susceptibility, virus-host interaction, or to identify viral virulence markers is a priority. Fish possess an effective antiviral response mediated by type I interferon, IFN I, a cytokine that induces the expression of a set of genes, called IFN-stimulated genes (ISGs), which generate an antiviral state in infected and surrounding cells. Among ISGs, mx genes are considered markers of the IFN I response, since Mx proteins show direct antiviral activity and mx genes display strong and quick induction, which varies according to viral virulence. In addition, mx transcription is blocked by several viruses. Therefore, the level and time-course of mx induction reflect virus-host interaction. This idea prompted the development of in vitro experimental systems consisting of RTG-2 cells stably expressing luciferase under the control of mx promoters to study virus-host interplay. Specifically, Senegalese sole and sea bream mx promoters have been evaluated in this study. Both systems were inoculated with different doses of viral isolates relevant in aquaculture: Infectious Pancreatic Necrosis Virus, IPNV, Viral Haemorrhagic Septicaemia Virus, VHSV, and Nervous Necrosis Virus, NNV. Each isolate triggered a characteristic profile in each experimental system. The sensitivity of both experimental systems in detecting two NNV isolates in infected tissues was tested, and the NNV antagonistic activity was also characterized. The minimal viral dose required to detect pmx induction and/or blocking is a possible virulence marker for these isolates. Thus, both experimental systems have been validated and can be used to get more insight on virus-host interaction and contribute to fight viral infections in aquaculture. Acknowledgments This study has been supported by the Project UMA20-FEDERJA-020 (Regional Government).Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Problemas matemáticos y personalidad

En este trabajo se lleva a cabo la aplicación de un modelo didáctico para formación de la personalidad propuesta por Sigarreta y Laborde (2003), favoreciendo en particular las cualidades denominadas perseverancia y espíritu crítico a través del tratamiento de los problemas matemáticos

Genomic characterization and transcription analysis of European sea bass (Dicentrarchus labrax) rtp3 genes.

Fish RTP3, belonging to the receptor-transporting protein family, display several functions, including a putative antiviral role as virus-responsive gene. In this work, we have identified and characterized two different European sea bass rtp3 genes. In addition, an in vivo transcription analysis in response to LPS, poly I:C and betanodavirus infection (RGNNV genotype) has been performed. The sequence analysis showed that European sea bass displays two rtp3 genes, X1 and X2, composed of two exons and a single intron (1007-bp and 888-bp long, respectively), located within the ORF sequence. The full-length cDNA is 1969 bp for rtp3 X1, and 1491 bp for rtp3 X2. Several ATTTA motifs have been found in the intron sequence of both genes, whereas rtp3 X1 also contains this motif in both untranslated regions. The transcription analyses revealed significant level of rtp3 X2 mRNA in brain and head kidney after LPS and poly I:C inoculation; however, the induction elicited by RGNNV infection was much higher, suggesting an essential role for this protein in controlling NNV infectionsFunding for open access charge: Universidad de Málaga / CBU

Inhibition of nervous necrosis virus replication by Shewanella putrefaciens Pdp11 extract

One of the most relevant infectious diseases affecting farmed fish is the viral encephalopathy and retinopathy, caused by the nervous necrosis virus (NNV). The two commercial vaccines available against this virus have been designed to protect European seabass against the infection with a particular NNV species, RGNNV. Therefore, the development of strategies to protect different fish species against different viruses is a key issue for the aquaculture industry. In this regard, there are numerous studies focused on the use of probiotics, although their antiviral activity has been poorly investigated. Shewanella putrefaciens Pdp11, SpPdp11, is a probiotic with proven positive effects on gilthead seabream and Senegalese sole, protecting those species against several bac- terial pathogens; however, its antiviral activity remains to be investigated. The current study is a step forward in the use of probiotics against viral infections, evaluating the anti-RGNNV activity of sonicated-SpPdp11 extracts both, in vitro and in vivo. According to our results, SpPdp11 extracts compromised RGNNV multiplication in E11 cells, affecting viral assembly and/or exit rather than genome replication. Furthermore, this antiviral activity may be produced by the capacity of the SpPdp11-extract to induce an immune response in treated cells. This antiRGNNV activity has been confirmed in vivo, since 82% of fish fed with the SpPdp11-supplemented diet survived an experimental-RGNNV infection, whereas the survival rate of fish fed with the control diet was 64%. These results suggest that SpPdp11-supplemented feeding can be a promising prophylactic tool against RGNNV and encourage further research on other fish species and viral pathogens.This study has been supported by the projects P18-RT-1067 (Proyecto de Excelencia, Junta de Andalucia, Regional Government) and PID2020-113637RB-C22 (MINECO/AEI/FEDER, UE) (Spanish Government). Daniel Álvarez-Torres has been granted by the project PTA2020-018984-I. Funding for open access charge: Universidad de Málaga/CBUA

Reducing the environmental impact of surgery on a global scale: systematic review and co-prioritization with healthcare workers in 132 countries

Abstract Background Healthcare cannot achieve net-zero carbon without addressing operating theatres. The aim of this study was to prioritize feasible interventions to reduce the environmental impact of operating theatres. Methods This study adopted a four-phase Delphi consensus co-prioritization methodology. In phase 1, a systematic review of published interventions and global consultation of perioperative healthcare professionals were used to longlist interventions. In phase 2, iterative thematic analysis consolidated comparable interventions into a shortlist. In phase 3, the shortlist was co-prioritized based on patient and clinician views on acceptability, feasibility, and safety. In phase 4, ranked lists of interventions were presented by their relevance to high-income countries and low–middle-income countries. Results In phase 1, 43 interventions were identified, which had low uptake in practice according to 3042 professionals globally. In phase 2, a shortlist of 15 intervention domains was generated. In phase 3, interventions were deemed acceptable for more than 90 per cent of patients except for reducing general anaesthesia (84 per cent) and re-sterilization of ‘single-use’ consumables (86 per cent). In phase 4, the top three shortlisted interventions for high-income countries were: introducing recycling; reducing use of anaesthetic gases; and appropriate clinical waste processing. In phase 4, the top three shortlisted interventions for low–middle-income countries were: introducing reusable surgical devices; reducing use of consumables; and reducing the use of general anaesthesia. Conclusion This is a step toward environmentally sustainable operating environments with actionable interventions applicable to both high– and low–middle–income countries

Notas : educación de personas adultas

El Departamento de Orientación se constituye oficialmente al implantarse la nueva EPA regulada por la LOGSE. Las crecientes necesidades de información, orientación o asesoramiento de cualquier persona adulta hacen que la actividad orientadora adquiera una mayor complejidad. Sin embargo, aún no está definido el rol profesional ni los ámbitos de funcionamiento del Departamento de Orientación o sus posibilidades de actuación. Así, las referencias al Departamento de Orientación de los centros de adultos, en aspectos como composición, funciones, actividades y ámbitos de actuación son escasas, si se comparan con el desarrollo legislativo experimentado por la orientación en los niveles de Secundaria, Infantil y Primaria. Las actuaciones se desarrollan en tres ámbitos: ayudar y acompañar al alumno en su proceso de aprendizaje; favorecer su integración en el grupo y el centro; y asesorar sobre aspectos relativos al desarrollo profesional. Una de las dificultades que limita la actuación del Departamento de Orientación respecto a la Educación Básica de Adultos es la necesidad de adaptar o contextualizar los planes a las características del alumnado, asumiendo y planificando colectivamente los objetivos y actuaciones del centro mediante la acción conjunta de orientadores, tutores y coordinadores docentes. Además, otras dificultades hacen referencia a las necesidades de aprendizaje de los alumnos; los contenidos procedimentales; el desarrollo de la acción tutorial teniendo en cuenta la diversidad del alumnado; o qué necesidades de información o asesoramiento tienen los alumnos y alumnas.MadridMadrid (Comunidad Autónoma). Servicio de Formación del Profesorado. CRIF Las Acacias; Calle General Ricardos, 179; 28025 Madrid; Tel. +34915250893; Fax +34914660991; [email protected]

Elaboración de materiales de expresión plástica

Trabajo en el que se recogen en forma de fichas actividades de expresión plástica, elaborados por un grupo de maestros de Educación Infantil. Cada ficha se acompaña de técnicas, materiales necesarios y fotografías. La publicación incluye plantillas para la confección de las distintas actividades: abanicos, tarjetas móviles, trabajos con arcilla, puzzles, etc..ExtremaduraES

New signatures of poor CD4 cell recovery after suppressive antiretroviral therapy in HIV-1-infected individuals: involvement of miR-192, IL-6, sCD14 and miR-144

Up to 40% of newly diagnosed cases of HIV-1 infection are late diagnoses, with a profound decrease in CD4 cell counts in many cases. One-third of these individuals do not achieve optimal CD4 cell recovery (OR) after suppressive antiretroviral treatment (ART). This retrospective/longitudinal study of poor recovery (PR) included 79 HIV-1-infected individuals with CD4 count <200 cells/mm3 (25 PR and 54 OR) before ART. After suppressive ART, 21 PR and 24 OR individuals were further analysed, including paired samples. Selected miRs and plasma inflammatory markers were determined to investigate their potential predictive/diagnostic value for poor recovery. miR-192, IL-6 and sCD14 were independently associated with CD4 recovery before ART (p = 0.031, p = 0.007, and p = 0.008, respectively). The combination of these three factors returned a good discrimination (predictive value for PR) value of 0.841 (AUC, p < 0.001). After suppressive ART, miR-144 was independently associated with CD4 recovery (p = 0.017), showing a moderate discrimination value of 0.730 (AUC, p = 0.008) for PR. Our study provides new evidence on the relationship between miRs and HIV-1 infection that could help improve the management of individuals at HIV-1 diagnosis. These miRs and cytokines signature sets provide novel tools to predict CD4 cell recovery and its progression after ART.This work was supported in part by the Spanish AIDS Network “Red de Investigación en Sida (RIS)” RD16/0025/0001; Ayudas contratación de ayudantes de investigación y técnicos de laboratorio, Consejería de Educación, Juventud y Deporte de la Comunidad de Madrid (PEJ15/bio/tl-0064 to MJRL and PEJ17/AI_BMD-6683 to FHW); Health Institute Carlos III, PI18/01216; Junta de Andalucía, Consejería de Economía, Innovación, Ciencia y Empleo, Proyecto de Investigación de Excelencia CTS2593; YMP was supported by the Consejería de Salud y Bienestar Social of Junta de Andalucía through the “Nicolás Monardes” programme (C-0013-2017)

Documentos de apoyo para la elaboración de las programaciones didácticas : Educación infantil. Educación Primaria. Educación Secundaria Obligatoria y Bachillerato

Las programaciones docentes deben ser el reflejo documental del plan de trabajo en cada área o materia, fruto de la reflexión conjunta de los miembros del equipo docente o departamento, que desde la reforma operada por la LOMCE deben recoger los nuevos elementos del currículo, como las competencias clave y los estándares de aprendizaje.Esta publicación se concibe como un documento de apoyo para los equipos de ciclo y departamentos, así como para los equipos directivos, en esta tarea de elaboración de las programaciones docentes anuales y contiene unas orientaciones generales, elementos de que debe constar, responsables y normativa de referencia. El documento se centra, de manera diferenciada en la Educación Infantil, en la Educación Primaria y en la Educación Secundaria Obligatoria y Bachillerato.ES