Development of Alternative Methods to Assess the Toxicity and Bioaccumulation Potential of Chemicals in the Aquatic Environment

The toxicity and bioaccumulation potential of chemicals are properties that need to be assessed in risk assessment. In the context of the aquatic environment, both properties were traditionally evaluated in the whole fish. However, due to the reluctance to use a large number of animals for experimentation and high cost of in vivo testing, alternative techniques have been developed to assess these properties. This thesis describes three distinct investigations towards the development of alternative methods for predicting the toxicity and bioaccumulation potential of chemicals. The first study of this thesis is centred on the development of a list of reference compounds to evaluate non-animal methods to in vivo bioaccumulation studies in fish. The selection of representative chemicals was developed following a novel strategy built from previous criteria proposed for the validation of experimental tests and considering relevant aspects for the bioaccumulation of organic chemicals. A revision and a comparison of the most used alternative approaches to in vivo bioaccumulation studies were undertaken in this thesis. In particular, a variety of in vitro and in silico methods were explored and compared in terms of their reliability to predict the whole body biotransformation rate and bioconcentration factor of chemicals in fish. As a consequence of this investigation, an insight into the main challenges and future perspectives for each of the methods evaluated was conducted to provide a foundation for future research. The last research study is focused on the verification of the prediction of protein binding for cyclic compounds and the development of a decision tree strategy to prioritise chemicals for in vivo toxicity testing. The last two objectives were developed based on the integration of different alternative methods to assess the toxicity of chemicals. This thesis concludes with a summary and a discussion of the work undertaken and suggestions for future work

Potential use of split-beam echosounder angular information for bivalve banks cartography: a critical evaluation

Peer Reviewe

Voltage dip generator for testing wind turbines connected to electrical networks

This paper describes a new voltage dip generator that allows the shape of the time profile of the voltage generated to be configured. The use of this device as a tool to test the fault ride-through capability of wind turbines connected to the electricity grid can provide some remarkable benefits: First, this system offers the possibility of adapting the main features of the time–voltage profile generated (dip depth, dip duration, the ramp slope during the recovery process after clearing fault, etc.) to the specific requirements set forth by the grid operation codes, in accordance with different network electrical systems standards. Second, another remarkable ability of this system is to provide sinusoidal voltage and current wave forms during the overall testing process without the presence of harmonic components. This is made possible by the absence of electronic converters. Finally, the paper includes results and a discussion on the experimental data obtained with the use of a reduced size laboratory prototype that was constructed to validate the operating features of this new device

Warps and correlations with intrinsic parameters of galaxies in the visible and radio

From a comparison of the different parameters of warped galaxies in the radio, and especially in the visible, we find that: a) No large galaxy (large mass or radius) has been found to have high amplitude in the warp, and there is no correlation of size/mass with the degree of asymmetry of the warp. b) The disc density and the ratio of dark to luminous mass show an opposing trend: smaller values give more asymmetric warps in the inner radii (optical warps) but show no correlation with the amplitude of the warp; however, in the external radii neither is there any correlation with the asymmetry. c) A third anticorrelation arises from a comparison of the amplitude and degree of asymmetry in the warped galaxies. Hence, it seems that very massive dark matter haloes have nothing to do with the formation of warps but only with the degree of symmetry in the inner radii, and are unrelated to the warp shape for the outermost radii. Denser discs show up the same dependence.Comment: 13 pages, 2 figures, accepted to be published in A&

Genome-scale co-expression network comparison across escherichia coli and salmonella enterica serovar typhimurium reveals significant conservation at the regulon level of local regulators despite their dissimilar lifestyles

Availability of genome-wide gene expression datasets provides the opportunity to study gene expression across different organisms under a plethora of experimental conditions. In our previous work, we developed an algorithm called COMODO (COnserved MODules across Organisms) that identifies conserved expression modules between two species. In the present study, we expanded COMODO to detect the co-expression conservation across three organisms by adapting the statistics behind it. We applied COMODO to study expression conservation/divergence between Escherichia coli, Salmonella enterica, and Bacillus subtilis. We observed that some parts of the regulatory interaction networks were conserved between E. coli and S. enterica especially in the regulon of local regulators. However, such conservation was not observed between the regulatory interaction networks of B. subtilis and the two other species. We found co-expression conservation on a number of genes involved in quorum sensing, but almost no conservation for genes involved in pathogenicity across E. coli and S. enterica which could partially explain their different lifestyles. We concluded that despite their different lifestyles, no significant rewiring have occurred at the level of local regulons involved for instance, and notable conservation can be detected in signaling pathways and stress sensing in the phylogenetically close species S. enterica and E. coli. Moreover, conservation of local regulons seems to depend on the evolutionary time of divergence across species disappearing at larger distances as shown by the comparison with B. subtilis. Global regulons follow a different trend and show major rewiring even at the limited evolutionary distance that separates E. coli and S. enterica

EVIDENT 3 Study: A randomized, controlled clinical trial to reduce inactivity and caloric intake in sedentary and overweight or obese people using a smartphone application: Study protocol

Introduction: Mobile technology, when included within multicomponent interventions, could contribute to more effective weight loss. The objective of this project is to assess the impact of adding the use of the EVIDENT 3 application, designed to promote healthy living habits, to traditional modification strategies employed for weight loss. Other targeted behaviors (walking, caloric-intake, sitting time) and outcomes (quality of life, inflammatory markers, measurements of arterial aging) will also be evaluated. Methods: Randomized, multicentre clinical trial with 2 parallel groups. The study will be conducted in the primary care setting and will include 700 subjects 20 to 65 years, with a body mass index (27.5-40kg/m2), who are clinically classified as sedentary. The primary outcome will be weight loss. Secondary outcomes will include change in walking (steps/d), sitting time (min/wk), caloric intake (kcal/d), quality of life, arterial aging (augmentation index), and pro-inflammatory marker levels. Outcomes will be measured at baseline, after 3 months, and after 1 year. Participants will be randomly assigned to either the intervention group (IG) or the control group (CG). Both groups will receive the traditional primary care lifestyle counseling prior to randomization. The subjects in the IG will be lent a smartphone and a smartband for a 3-month period, corresponding to the length of the intervention. The EVIDENT 3 application integrates the information collected by the smartband on physical activity and the self-reported information by participants on daily food intake. Using this information, the application generates recommendations and personalized goals for weight loss. Discussion: There is a great diversity in the applications used obtaining different results on lifestyle improvement and weight loss. The populations studied are not homogeneous and generate different results. The results of this study will help our understanding of the efficacy of new technologies, combined with traditional counseling, towards reducing obesity and enabling healthier lifestyles. Ethicsanddissemination: The study was approved by the Clinical Research Ethics Committee of the Health Area of Salamanca ("CREC of Health Area of Salamanca") on April 2016. A SPIRIT checklist is available for this protocol. The trial was registered in ClinicalTrials.gov provided by the US National Library of Medicine-number NCT03175614

Influencia de los estilos de vida físicamente activos y sedentarios en la percepción subjetiva de la salud y el bienestar de la juventud española

La presente comunicación se enmarca en un estudio financiado por el Consejo Superior de Deportes (CSD) (Ref. 007/UPB10/12). Uno de los objetivos específicos del estudio, en el que se basa la comunicación, es ampliar el conocimiento sobre la influencia de los estilos de vida físicamente activos y sedentarios en la percepción subjetiva de la salud y el bienestar de la juventud española

Fossil group origins V. The dependence of the luminosity function on the magnitude gap

In nature we observe galaxy aggregations that span a wide range of magnitude gaps between the two first-ranked galaxies of a system ($\Delta m_{12}$). There are systems with gaps close to zero (e.g., the Coma cluster), and at the other extreme of the distribution, the largest gaps are found among the so-called fossil systems. Fossil and non-fossil systems could have different galaxy populations that should be reflected in their luminosity functions. In this work we study, for the first time, the dependence of the luminosity function parameters on $\Delta m_{12}$ using data obtained by the fossil group origins (FOGO) project. We constructed a hybrid luminosity function for 102 groups and clusters at $z \le 0.25$. We stacked all the individual luminosity functions, dividing them into bins of $\Delta m_{12}$, and studied their best-fit Schechter parameters. We additionally computed a relative luminosity function, expressed as a function of the central galaxy luminosity, which boosts our capacity to detect differences, especially at the bright end. We find trends as a function of $\Delta m_{12}$ at both the bright and faint ends of the luminosity function. In particular, at the bright end, the larger the magnitude gap, the fainter the characteristic magnitude $M^\ast$. We also find differences at the faint end. In this region, the larger the gap, the flatter the faint-end slope $\alpha$. The differences found at the bright end support a dissipationless, dynamical friction-driven merging model for the growth of the central galaxy in group- and cluster-sized halos. The differences in the faint end cannot be explained by this mechanism. Other processes, such as enhanced tidal disruption due to early infall and/or prevalence of eccentric orbits, may play a role. However, a larger sample of systems with $\Delta m_{12} > 1.5$ is needed to establish the differences at the faint end.Comment: 11 pages, 10 figures, accepted for publication in A&

Stratospheric aircraft exhaust plume and wake chemistry studies

This report documents progress to date in an ongoing study to analyze and model emissions leaving a proposed High Speed Civil Transport (HSCT) from when the exhaust gases leave the engine until they are deposited at atmospheric scales in the stratosphere. Estimates are given for the emissions, summarizing relevant earlier work (CIAP) and reviewing current propulsion research efforts. The chemical evolution and the mixing and vortical motion of the exhaust are analyzed to track the exhaust and its speciation as the emissions are mixed to atmospheric scales. The species tracked include those that could be heterogeneously reactive on the surfaces of the condensed solid water (ice) particles and on exhaust soot particle surfaces. Dispersion and reaction of chemical constituents in the far wake are studied with a Lagrangian air parcel model, in conjunction with a radiation code to calculate the net heating/cooling. Laboratory measurements of heterogeneous chemistry of aqueous sulfuric acid and nitric acid hydrates are also described. Results include the solubility of HCl in sulfuric acid which is a key parameter for modeling stratospheric processing. We also report initial results for condensation of nitric acid trihydrate from gas phase H2O and HNO3