Immunosensors: Concepts and Structures for Fast and Accurate Sensing

Peer ReviewedPostprint (published version

The discovery of CRISPR in archaea and bacteria

CRISPR-Cas are self-/nonself-discriminating systems found in prokaryotic cells. They represent a remarkable example of molecular memory that is hereditarily transmitted. Their discovery can be considered as one of the first fruits of the systematic exploration of prokaryotic genomes. Although this genomic feature was serendipitously discovered in molecular biology studies, it was the availability of multiple complete genomes that shed light about their role as a genetic immune system. Here we tell the story of how this discovery originated and was slowly and painstakingly advanced to the point of understating the biological role of what initially was just an odd genomic feature.FJMM is funded by the Spanish Ministerio de Economía y Competitividad (BIO2014-53029P) and the European Commission/Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (291815 Era-Net ANIHWA). FR-V is funded by projects MEDIMAX BFPU2013-48007-P from the Spanish Ministerio de Economía y Competitividad, MaCuMBA Project 311975 of the European Commission FP7 and PROMETEO II/2014/012 project AQUAMET from the Generalitat Valenciana

¿Cuál es la percepción del profesorado de la motivación de los alumnos de Primaria?

Treball Final de Grau en Mestre o Mestra d'Educació Primària. Codi: MP1040. Curs acadèmic: 2017/2018Este trabajo se ha realizado a partir del Modelo de Calidad de Situación Educativa (Doménech, 2018). Tiene como objetivo analizar la percepción que tiene el profesorado de primaria de sus apoyos/barreras personales y de los apoyos/barreras contextuales que pueden condicionar el tipo de estrategias motivacionales que éstos utilizan en el aula. La muestra está formada por 52 profesores de quinto y sexto de primaria de diferentes colegios de la Comunidad Valenciana. Ellos colaboraron en la investigación rellenando un cuestionario de manera anónima y voluntaria. Se ha realizado un análisis de los datos y se ha observado que el profesorado piensa que está bastante formado en motivación. También se ha observado que tanto los profesores como las familias tienen el poder de mejorar la motivación de los alumnos. Por otro lado, se ha detectado que el profesorado manifiesta tener bastantes apoyos para motivar al alumnado y los datos indican que el número de barreras también es alto. Partiendo de los resultados obtenidos, se han realizado una serie de propuestas de mejora para disminuir las dificultades que pueden tener los profesores en sus aulasThis work has been realized from the Quality Model Educational (Doménech, 2018). Its objective is to analyze the perception that the primary school teachers have of their personal supports/barriers and of the contextual supports/barriers that can condition the type of motivational strategies that they use in the classroom. The sample is formed by 52 teachers of fifth and sixth of primary of different schools of the Valencian Community. They collaborated in the research filling a questionnaire of an anonymous and voluntary way. It has been realized an analysis of the information and it has been observed that the teachers think they enough are formed in motivation. Also it has been observed that both teachers and families have the power to improve the pupils motivation. On the other hand, it has been detected that the teachers says they have enough supports to motivate children and the information indicate that the number of barriers also is high. Based on the results obtained, it have been realized a series of improvement proposals to reduce the difficulties that the teachers can have in his classrooms

Proyecto de intervención sobre parentalidad positiva para familias cuyos hijos e hijas presentan dificultades psicopedagógicas

Diversos autores han afirmado la importancia de ejercer una parentalidad positiva en familias cuyos hijos presentan dificultades educativas, emocionales, conductuales…para reducir las repercusiones de las mismas en el desempeño parental. En este estudio, se pretende concienciar sobre las necesidades reales que se presentan en tales contextos familiares, ayudando a mejorar su situación. Se ha elaborado un proyecto de intervención para dichas familias. Para justificar el programa y detectar las necesidades reales de estos contextos, se ha llevado a cabo una investigación cualitativa cuyo método de recogida de información ha sido la entrevista a 9 usuarios/as del gabinete psicopedagógico Educadoss en Sevilla (Andalucía, España). Los resultados han desvelado una carencia formativa de habilidades y competencias parentales frente a problemas comportamentales, emocionales o educativos de sus hijos que dificultan, así, la práctica educativa o el ejercicio de educar. Sin embargo, en contraposición es relevante el alto grado de implicación familiar que existe en las mismas. Los resultados concuerdan con otras investigaciones sobre obstáculos que impiden el desarrollo parental positivo en tales contextos y las necesidades que se plantean. Se aportan evidencias que permiten justificar el proyecto y abrir nuevas líneas de intervención e investigación sobre parentalidad positiva.Several authors have affirmed the importance of exercising positive parenting in families whose children present educational, emotional, behavioural difficulties ... to reduce the repercussions of them in their parenting role. In this article, it aims to raise awareness about the real needs that arise in such family contexts, helping to improve their situation. An intervention project has been designed for these families. Due to justifying the program and detecting the real necessities of these contexts, a qualitative investigation was carried out using an interview as a method of collecting information Trabajo Fin de Grado Pedagogía US 2016/17 | Julia Rodriguez Valera 6 made on a 9-people sample part of the psycho-pedagogical center called Educadoss in Seville (Andalusia, Spain). Results have revealed a lack of formative and parental skills related with the emotional, behavioural and educational problems their children have, that complicate their educational practice. However, the prominent degree of family involvement is relevant. These results concur with other researches about the obstacles positive parental development has in such contexts and the necessities exposed. Evidences are provided to justify the project and to open new lines of interventions and researches related to the positive parenting.Universidad de Sevilla. Grado en Pedagogí

Proyecto de intervención sobre parentalidad positiva para familias cuyos hijos e hijas presentan dificultades psicopedagógicas

Diversos autores han afirmado la importancia de ejercer una parentalidad positiva en familias cuyos hijos presentan dificultades educativas, emocionales, conductuales…para reducir las repercusiones de las mismas en el desempeño parental. En este estudio, se pretende concienciar sobre las necesidades reales que se presentan en tales contextos familiares, ayudando a mejorar su situación. Se ha elaborado un proyecto de intervención para dichas familias. Para justificar el programa y detectar las necesidades reales de estos contextos, se ha llevado a cabo una investigación cualitativa cuyo método de recogida de información ha sido la entrevista a 9 usuarios/as del gabinete psicopedagógico Educadoss en Sevilla (Andalucía, España). Los resultados han desvelado una carencia formativa de habilidades y competencias parentales frente a problemas comportamentales, emocionales o educativos de sus hijos que dificultan, así, la práctica educativa o el ejercicio de educar. Sin embargo, en contraposición es relevante el alto grado de implicación familiar que existe en las mismas. Los resultados concuerdan con otras investigaciones sobre obstáculos que impiden el desarrollo parental positivo en tales contextos y las necesidades que se plantean. Se aportan evidencias que permiten justificar el proyecto y abrir nuevas líneas de intervención e investigación sobre parentalidad positiva.Several authors have affirmed the importance of exercising positive parenting in families whose children present educational, emotional, behavioural difficulties ... to reduce the repercussions of them in their parenting role. In this article, it aims to raise awareness about the real needs that arise in such family contexts, helping to improve their situation. An intervention project has been designed for these families. Due to justifying the program and detecting the real necessities of these contexts, a qualitative investigation was carried out using an interview as a method of collecting information Trabajo Fin de Grado Pedagogía US 2016/17 | Julia Rodriguez Valera 6 made on a 9-people sample part of the psycho-pedagogical center called Educadoss in Seville (Andalusia, Spain). Results have revealed a lack of formative and parental skills related with the emotional, behavioural and educational problems their children have, that complicate their educational practice. However, the prominent degree of family involvement is relevant. These results concur with other researches about the obstacles positive parental development has in such contexts and the necessities exposed. Evidences are provided to justify the project and to open new lines of interventions and researches related to the positive parenting.Universidad de Sevilla. Grado en Pedagogí

Characterization of a piezoelectric acoustic sensor fabricated for low-frequency applications: a comparative study of three methods

Piezoelectric transducers are widely used for generating acoustic energy, and choosing the right radiating element is crucial for efficient energy conversion. In recent decades, numerous studies have been conducted to characterize ceramics based on their elastic, dielectric, and electromechanical properties, which have improved our understanding of their vibrational behavior and aided in the manufacturing of piezoelectric transducers for ultrasonic applications. However, most of these studies have focused on the characterization of ceramics and transducers using electrical impedance to obtain resonance and anti-resonance frequencies. Few studies have explored other important quantities such as acoustic sensitivity using the direct comparison method. In this work, we present a comprehensive study that covers the design, manufacturing, and experimental validation of a small-sized, easy-to-assemble piezoelectric acoustic sensor for low-frequency applications, using a soft ceramic PIC255 from PI Ceramic with a diameter of 10 mm and a thickness of 5 mm. We present two methods, analytical and numerical, for sensor design, followed by experimental validation, allowing for a direct comparison of measurements with simulated results. This work provides a useful evaluation and characterization tool for future applications of ultrasonic measurement systems.This workwas supported by the grants PID2020-116329GB-C22 and TED2021-129336B-I00 funded by MCIN/AEI/10.13039/501100011033 and by the European Union NextGenerationEU/PRTR. This work is also a result of an internship funded by the Autonomous Community of the Región of Murcia through the Fundación Séneca—Agencia de Ciencia y Tecnología de la Región de Murcia (Seneca Foundation—Agency for Science and Technology in the Region of Murcia) and the European Union’s NextGenerationEU program

Light as an energy source in continuous cultures of bacteriorhodopsin-containing halobacteria

The role of light as an energy source for slightly aereated cultures of halobac­ teria was studied, using continuous cultures with low nutrient concentrations and a low oxygen supply. A series of experiments were carried out with non­ illuminated and differently illuminated cultures and with ditferent oxygen transfer rates. Under low oxygen availability, light proved to be a decisively important energy source that allowed the populations to reach higher growth rates and much higher population densities. Oxygen inftuenced the growth over only a minimal level, below which neither the illuminated nor the dark cultures were atfected by the oxygen transfer rate. From these results, it appears that the bacteriorhodop­ sin-mediated energy supply could have a very important role for the ecology of halobacteria in their microaerophilic habitats. In the illuminated cultures, cells that originated purple colonies on plates appeared. These cells, which could be bacteriorhodopsin-constitutive mutants, are now being studied

Micro-Mar: a database for dynamic representation of marine microbial biodiversity

BACKGROUND: The cataloging of marine prokaryotic DNA sequences is a fundamental aspect for bioprospecting and also for the development of evolutionary and speciation models. However, large amount of DNA sequences used to quantify prokaryotic biodiversity requires proper tools for storing, managing and analyzing these data for research purposes. DESCRIPTION: The Micro-Mar database has been created to collect DNA diversity information from marine prokaryotes for biogeographical and ecological analyses. The database currently includes 11874 sequences corresponding to high resolution taxonomic genes (16S rRNA, ITS and 23S rRNA) and many other genes including CDS of marine prokaryotes together with available biogeographical and ecological information. CONCLUSION: The database aims to integrate molecular data and taxonomic affiliation with biogeographical and ecological features that will allow to have a dynamic representation of the marine microbial diversity embedded in a user friendly web interface. It is available online at