Regulation by Gonadal Steroids of Estrogen and Progesterone Receptors Along the Reproductive Tract in Female Lambs

The regulation of estrogen and progesterone receptor (ER, PR) expression by estradiol (E2) and progesterone (P4) in the oviduct, uterus and cervix of female lambs was studied. The animals received three intramuscular injections of E2, P4 or vehicle with an interval of 24 h and they were slaugthered 24 h after the third injection. Determinations of ER and PR were performed by binding assays and mRNAs of ERα and PR by solution hybridization. High levels of ER and PR in both cervix and oviduct were found in the female lamb, differing from other mammalian species. No significant effects by either E2 or P4 treatment on ER and PR levels in the cervix and oviduct could be observed. E2 treatment increased the mRNA levels of ERa and PR more than 3-fold in the cervix, while P4 treatment increased the mRNA levels of ERa and PR in the uterus. The results show differential effects of gonadal steroids on sex steroid receptor expression along the reproductive tract in female lambs, suggesting that steroid target tissues can modulate responses to the same circulating levels of steroid hormones

Looking for a Better Characterization of Triple-Negative Breast Cancer by Means of Circulating Tumor Cells

Traditionally, studies to address the characterization of mechanisms promoting tumor aggressiveness and progression have been focused only on primary tumor analyses, which could provide relevant information but have limitations to really characterize the more aggressive tumor population. To overcome these limitations, circulating tumor cells (CTCs) represent a noninvasive and valuable tool for real-time profiling of disseminated tumor cells. Therefore, the aim of the present study was to explore the value of CTC enumeration and characterization to identify markers associated with the outcome and the aggressiveness of triple-negative breast cancer (TNBC). For that aim, the CTC population from 32 patients diagnosed with TNBC was isolated and characterized. This population showed important cell plasticity in terms of expression of epithelia/mesenchymal and stemness markers, suggesting the relevance of epithelial to mesenchymal transition (EMT) intermediate phenotypes for efficient tumor dissemination. Importantly, the CTC signature demonstrated prognostic value to predict the patients' outcome and pointed to a relevant role of tissue inhibitor of metalloproteinases 1 (TIMP1) and androgen receptor (AR) for TNBC biology. Furthermore, we also analyzed the usefulness of the AR and TIMP1 blockade to target TNBC proliferation and dissemination using in vitro and in vivo zebra fish and mouse models. Overall, the molecular characterization of CTCs from advanced TNBC patients identifies highly specific biomarkers with potential applicability as noninvasive prognostic markers and reinforced the value of TIMP1 and AR as potential therapeutic targets to tackle the most aggressive breast cancer

Ahora / Ara

La cinquena edició del microrelatari per l’eradicació de la violència contra les dones de l’Institut Universitari d’Estudis Feministes i de Gènere «Purificación Escribano» de la Universitat Jaume I vol ser una declaració d’esperança. Aquest és el moment en el qual les dones (i els homes) hem de fer un pas endavant i eliminar la violència sistèmica contra les dones. Ara és el moment de denunciar el masclisme i els micromasclismes començant a construir una societat més igualitària. Cadascun dels relats del llibre és una denúncia i una declaració que ens encamina cap a un món millor

Concentrations (fmol/mg protein) by ligand-binding assay of A) estrogen (ER) and B) progesterone (PR) receptor in the cervix and oviduct of 2 months old lambs after i

<p><b>Copyright information:</b></p><p>Taken from "Regulation by Gonadal Steroids of Estrogen and Progesterone Receptors Along the Reproductive Tract in Female Lambs"</p><p>Acta Veterinaria Scandinavica 2001;42(1):161-169.</p><p>Published online 31 Mar 2001</p><p>PMCID:PMC2202335.</p><p></p>m. injections of oil (Group C), estradiol (Group E) or progesterone (Group P). The bars represent least square mean ± SEM. There were no significant differences between the treatments

Lycopene/Arabinoxylan Gels: Rheological and Controlled Release Characteristics

molecule

COMPORTAMIENTO DE GENOTIPOS DE LA FAMILIA SOLANACEAE FRENTE A Meloidogyne incognita (KOFOID Y WHITE) CHITWOOD1

Los nematodos formadores de agallas (Meloidogyne spp.) constituyen factores limitantes de los rendimientos en la producción de hortalizas en campo abierto y casas de cultivo, donde el tomate(Solanum lycopersicum L. (ex Lycopersicon esculentum Mill)) representa un buen hospedante de estos nematodos. Para manejar las poblaciones de estos organismos, entre otras tácticas se hace uso de genotipos resistentes o se acude al injerto herbáceo para obtener resultados productivos favorables, resultando necesaria la evaluación de los genotipos a emplear frente a poblaciones nativas, pues se sabe que la resistencia puede ser quebrada por factores tales como la virulencia de la población de Meloidogyne. El objetivo de este estudio fue determinar el comportamiento de líneas, híbridos y variedades de tomate ante una población nativa de M. incognita raza 2, como elemento básico del programa de injerto herbáceo que se desarrolla en Cuba para contar con alternativas al bromuro de metilo. Se inocularon 10 plantas por genotipo, con 1,5 huevos/juveniles.g de suelo-1 y se evaluaron a los 60 días, determinándose el Índice de Agallamiento (IA), Factor de Reproducción (FR) e índice de reproducción (IR). Los cultivares evaluados fueron Lycopersicon peruvianum acc. B6-140; Physalis ixocarpa acc. B6236; Lycopersicon pimpinelifolium acc. Mex 121-A; las variedades/híbridos de S. lycopersicum var. cerasiforme, Vyta, Mariela, Amalia, HC 3880 y HA 3019; el híbrido inter-específico Lycopersicon hirsutum x L. esculentum, Solanum torvum y los híbridos de tomate T-1x14, T-1x15, T- 2x16 y LTM. Resultó inmune L. hirsutum x L. esculentum y muy resistentes S. torvum y L. peruvianum. Por su parte, P. ixocarpa se comportó como moderadamente resistente y el resto de los materiales evaluados fueron susceptibles a la población cubana de M. incognita raza 2.(Palabras clave: Meloidogyne incognita; resistencia; susceptibilidad; Solanaceae; injerto