Muon-induced neutron production and detection with GEANT4 and FLUKA

We report on a comparison study of the Monte Carlo packages GEANT4 and FLUKA for simulating neutron production by muons penetrating deep underground. GEANT4 is found to generate fewer neutrons at muon energies above ~100 GeV, by at most a factor of 2 in some materials, which we attribute mainly to lower neutron production in hadronic cascades. As a practical case study, the muon-induced neutron background expected in a 250 kg liquid-xenon WIMP dark matter detector was calculated and good agreement was found for the recoil event rates. The detailed model of neutron elastic scattering in GEANT4 was also shown to influence the nuclear recoil spectrum observed in the target, which is presently a shortcoming of FLUKA. We conclude that both packages are suited for this type of simulation, although further improvements are desirable in both cases.Comment: (23 pages, 14 figures) To appear in Nucl. Instrum. Meth. A v2: Changes to format only; v3: Corrected typo in front matter; v4: Looked up additional experimental data for comparison with simulation

MacB ABC transporter is a dimer whose ATPase activity and macrolide-binding capacity are regulated by the membrane fusion protein MacA

Gram-negative bacteria utilize specialized machinery to translocate drugs and protein toxins across the inner and outer membranes, consisting of a tripartite complex composed of an inner membrane secondary or primary active transporter (IMP), a periplasmic membrane fusion protein, and an outer membrane channel. We have investigated the assembly and function of the MacAB/TolC system that confers resistance to macrolides in Escherichia coli. The membrane fusion protein MacA not only stabilizes the tripartite assembly by interacting with both the inner membrane protein MacB and the outer membrane protein TolC, but also has a role in regulating the function of MacB, apparently increasing its affinity for both erythromycin and ATP. Analysis of the kinetic behavior of ATP hydrolysis indicated that MacA promotes and stabilizes the ATP-binding form of the MacB transporter. For the first time, we have established unambiguously the dimeric nature of a noncanonic ABC transporter, MacB that has an N-terminal nucleotide binding domain, by means of nondissociating mass spectrometry, analytical ultracentrifugation, and atomic force microscopy. Structural studies of ABC transporters indicate that ATP is bound between a pair of nucleotide binding domains to stabilize a conformation in which the substrate-binding site is outward-facing. Consequently, our data suggest that in the presence of ATP the same conformation of MacB is promoted and stabilized by MacA. Thus, MacA would facilitate the delivery of drugs by MacB to TolC by enhancing the binding of drugs to it and inducing a conformation of MacB that is primed and competent for binding TolC. Our structural studies are an important first step in understanding how the tripartite complex is assembled

Simulation of neutrons produced by high-energy muons underground

This article describes the Monte Carlo simulation used to interpret the measurement of the muon-induced neutron flux in the Boulby Underground Laboratory (North Yorkshire, UK), recently performed using a large scintillator veto deployed around the ZEPLIN-II WIMP detector. Version 8.2 of the GEANT4 toolkit was used after relevant benchmarking and validation of neutron production models. In the direct comparison between Monte Carlo and experimental data, we find that the simulation produces a 1.8 times higher neutron rate, which we interpret as over-production in lead by GEANT4. The dominance of this material in neutron production allows us to estimate the absolute neutron yield in lead as (1.31 +/- 0.06) x 10^(-3) neutrons/muon/(g/cm^2) for a mean muon energy of 260 GeV. Simulated nuclear recoils due to muon-induced neutrons in the ZEPLIN-II target volume (~1 year exposure) showed that, although a small rate of events is expected from this source of background in the energy range of interest for dark matter searches, no event survives an anti-coincidence cut with the veto.Comment: 13 Pages, 11 Figures, 3 Tables. To appear in Astroparticle Physics. Version 2 has minor corrections and clarifications. Figures 1 and 3 now include neutron yields obtained with FLUKA-200

Stakeholder narratives on trypanosomiasis, their effect on policy and the scope for One Health

Background This paper explores the framings of trypanosomiasis, a widespread and potentially fatal zoonotic disease transmitted by tsetse flies (Glossina species) affecting both humans and livestock. This is a country case study focusing on the political economy of knowledge in Zambia. It is a pertinent time to examine this issue as human population growth and other factors have led to migration into tsetse-inhabited areas with little historical influence from livestock. Disease transmission in new human-wildlife interfaces such as these is a greater risk, and opinions on the best way to manage this are deeply divided. Methods A qualitative case study method was used to examine the narratives on trypanosomiasis in the Zambian policy context through a series of key informant interviews. Interviewees included key actors from international organisations, research organisations and local activists from a variety of perspectives acknowledging the need to explore the relationships between the human, animal and environmental sectors. Principal Findings Diverse framings are held by key actors looking from, variously, the perspectives of wildlife and environmental protection, agricultural development, poverty alleviation, and veterinary and public health. From these viewpoints, four narratives about trypanosomiasis policy were identified, focused around four different beliefs: that trypanosomiasis is protecting the environment, is causing poverty, is not a major problem, and finally, that it is a Zambian rather than international issue to contend with. Within these narratives there are also conflicting views on the best control methods to use and different reasoning behind the pathways of response. These are based on apparently incompatible priorities of people, land, animals, the economy and the environment. The extent to which a One Health approach has been embraced and the potential usefulness of this as a way of reconciling the aims of these framings and narratives is considered throughout the paper. Conclusions/Significance While there has historically been a lack of One Health working in this context, the complex, interacting factors that impact the disease show the need for cross-sector, interdisciplinary decision making to stop rival narratives leading to competing actions. Additional recommendations include implementing: surveillance to assess under-reporting of disease and consequential under-estimation of disease risk; evidence-based decision making; increased and structurally managed funding across countries; and focus on interactions between disease drivers, disease incidence at the community level, and poverty and equity impacts

The orbifold transform and its applications

We discuss the notion of the orbifold transform, and illustrate it on simple examples. The basic properties of the transform are presented, including transitivity and the exponential formula for symmetric products. The connection with the theory of permutation orbifolds is addressed, and the general results illustrated on the example of torus partition functions

Nuclear receptors of the honey bee: annotation and expression in the adult brain

The Drosophila genome encodes 18 canonical nuclear receptors. All of the Drosophila nuclear receptors are here shown to be present in the genome of the honey bee (Apis mellifera). Given that the time since divergence of the Drosophila and Apis lineages is measured in hundreds of millions of years, the identification of matched orthologous nuclear receptors in the two genomes reveals the fundamental set of nuclear receptors required to ‘make’ an endopterygote insect. The single novelty is the presence in the A. mellifera genome of a third insect gene similar to vertebrate photoreceptor-specific nuclear receptor (PNR). Phylogenetic analysis indicates that this novel gene, which we have named AmPNR-like, is a new member of the NR2 subfamily not found in the Drosophila or human genomes. This gene is expressed in the developing compound eye of the honey bee. Like their vertebrate counterparts, arthropod nuclear receptors play key roles in embryonic and postembryonic development. Studies in Drosophila have focused primarily on the role of these transcription factors in embryogenesis and metamorphosis. Examination of an expressed sequence tag library developed from the adult bee brain and analysis of transcript expression in brain using in situ hybridization and quantitative RT-PCR revealed that several members of the nuclear receptor family (AmSVP, AmUSP, AmERR, AmHr46, AmFtz-F1, and AmHnf-4) are expressed in the brain of the adult bee. Further analysis of the expression of AmUSP and AmSVP in the mushroom bodies, the major insect brain centre for learning and memory, revealed changes in transcript abundance and, in the case of AmUSP, changes in transcript localization, during the development of foraging behaviour in the adult. Study of the honey bee therefore provides a model for understanding nuclear receptor function in the adult brain

Seismology of the Sun : Inference of Thermal, Dynamic and Magnetic Field Structures of the Interior

Recent overwhelming evidences show that the sun strongly influences the Earth's climate and environment. Moreover existence of life on this Earth mainly depends upon the sun's energy. Hence, understanding of physics of the sun, especially the thermal, dynamic and magnetic field structures of its interior, is very important. Recently, from the ground and space based observations, it is discovered that sun oscillates near 5 min periodicity in millions of modes. This discovery heralded a new era in solar physics and a separate branch called helioseismology or seismology of the sun has started. Before the advent of helioseismology, sun's thermal structure of the interior was understood from the evolutionary solution of stellar structure equations that mimicked the present age, mass and radius of the sun. Whereas solution of MHD equations yielded internal dynamics and magnetic field structure of the sun's interior. In this presentation, I review the thermal, dynamic and magnetic field structures of the sun's interior as inferred by the helioseismology.Comment: To be published in the proceedings of the meeting "3rd International Conference on Current Developments in Atomic, Molecular, Optical and Nano Physics with Applications", December 14-16, 2011, New Delhi, Indi

Assessing uncertainty in housing stock infiltration rates and associated heat loss: English and UK case studies

Strategies to reduce domestic heating loads by minimizing the infiltration of cold air through adventitious openings located in the thermal envelopes of houses are highlighted by the building codes of many countries. Consequent reductions of energy demand and CO2e emission are often unquantified by empirical evidence. Instead, a mean heating season infiltration rate is commonly inferred from an air leakage rate using a simple ratio scaled to account for the physical and environmental properties of a dwelling. The scaling does not take account of the permeability of party walls in conjoined dwellings and so cannot differentiate between the infiltration of unconditioned ambient air that requires heating, and conditioned air from adjacent dwellings that does not. A stochastic method is presented that applies a theoretical model of adventitious infiltration to predict distributions of mean infiltration rates and the associated total heat loss in any stock of dwellings during heating hours. The method is applied to the English and UK housing stocks and provides probability distribution functions of stock infiltration rates and total heat loss during the heating season for two extremes of party wall permeability. The distributions predict that up to 79% of the current English stock could require additional purpose-provided ventilation to limit negative health consequences. National models predict that fewer dwellings are under-ventilated. The distributions are also used to predict that infiltration is responsible for 3–5% of total UK energy demand, 11–15% of UK housing stock energy demand, and 10–14% of UK housing stock carbon emissions