incremental sheet forming for prototyping automotive modules

Abstract Actually, in many industrial applications, like automotive industry, mass decreases and fuel economy constitute very important features. Light alloys are extensively used in automotive applications and simultaneously, the constant industrial progress forces low-cost innovative machining routes to be used after the manufacturing process oriented to transform the product into a right shape with high accuracy of the geometry and good surface quality. In this paper, a general overview of the actual state-of- the-art about the Incremental Sheet Forming (ISF) is presented including some experimentally results of the current research in this field performed by the authors. The target of the research is oriented on the validation of the possibility to use a common milling machine, as ISF apparatus, and to find the most suitable process parameters to be assumed for the production of automotive sheet made by Ti alloys

Large-scale retrospective relative spectro-photometric self-calibration in space

We consider the application of relative self-calibration using overlap regions to spectroscopic galaxy surveys that use slit-less spectroscopy. This method is based on that developed for the SDSS by Padmanabhan at al. (2008) in that we consider jointly fitting and marginalising over calibrator brightness, rather than treating these as free parameters. However, we separate the calibration of the detector-to-detector from the full-focal-plane exposure-to-exposure calibration. To demonstrate how the calibration procedure will work, we simulate the procedure for a potential implementation of the spectroscopic component of the wide Euclid survey. We study the change of coverage and the determination of relative multiplicative errors in flux measurements for different dithering configurations. We use the new method to study the case where the flat-field across each exposure or detector is measured precisely and only exposure-to-exposure or detector-to-detector variation in the flux error remains. We consider several base dither patterns and find that they strongly influence the ability to calibrate, using this methodology. To enable self-calibration, it is important that the survey strategy connects different observations with at least a minimum amount of overlap, and we propose an "S"-pattern for dithering that fulfills this requirement. The final survey strategy adopted by Euclid will have to optimise for a number of different science goals and requirements. The large-scale calibration of the spectroscopic galaxy survey is clearly cosmologically crucial, but is not the only one.Comment: 23 pages, 19 figures, Accepted for publication in MNRAS, 201

Gold-Nanoparticle-Based Colorimetric Discrimination of Cancer-Related Point Mutations with Picomolar Sensitivity

Point mutations in the Kirsten rat sarcoma viral oncogene homologue (KRAS) gene are being increasingly recognized as important diagnostic and prognostic markers in cancer. In this work, we describe a rapid and low-cost method for the naked-eye detection of cancer-related point mutations in KRAS based on gold nanoparticles. This simple colorimetric assay is sensitive (limit of detection in the low picomolar range), instrument-free, and employs nonstringent room temperature conditions due to a combination of DNA-conjugated gold nanoparticles, a probe design which exploits cooperative hybridization for increased binding affinity, and signal enhancement on the surface of magnetic beads. Additionally, the scheme is suitable for point-of-care applications, as it combines naked-eye detection, small sample volumes, and isothermal (PCR-free) amplification

Investigation of Mechanical Properties of Aluminum Tailor Welded Blanks

Nowadays, the reduction of CO2 emissions and the decrease in energy consumption are the main aims of several industries, especially in the automotive sector. To comply with the increasingly restrictive regulations, the automotive industry is constantly looking for innovative techniques to produce lighter, more efficient, and less polluting vehicles. One of the latest technologies, and still developing, is based on the fabrication of the body-in-white and car parts through the stamping of aluminum Tailor Welded Blanks. Tailor Welded Blanks (TWBs) are generally the combination of two/three metal sheets with different thicknesses and/or mechanical strengths, which are commonly butt-welded together by laser sources. The use of aluminum TWBs has several advantages such as low density and corrosion resistance adequate. However, their use is still limited by the lower formability with respect to the parent materials and the more intrinsic difficulty of laser welding of aluminum sheets (i.e., internal porosity) that, although its use in automated industries is constantly growing, remains a process to be further developed and improved. This study has investigated the effect of the main laser welding process parameters (laser power, welding speed, and focal distance) on the mechanical properties of aluminum TWBs made of 6xxx series. The research results show that a narrow weldability window can be found to ensure welded joints with high strength and limited or no porosity

Toxicity of citrate-capped AuNPs: an in vitro and in vivo assessment

In this study, we show that 15 nm citrate-capped AuNPs exert a remarkable toxicity in living systems. The assessment was performed by using well-characterized AuNPs, the combination of in vitro and in vivo models (namely two different cell lines and Drosophila melanogaster), exposure to low dosages of nanoparticles (in the sub-nanomolar concentration range), along with the application of several biological assays to monitor different aspects of the toxic effects, such as viability, genotoxicity, and molecular biomarkers

A trophic model of the benthopelagic fauna distributed in the Santa Maria di Leuca cold-water coral province (Mediterranean Sea)

The benthopelagic fauna distributed in and around the Santa Maria di Leuca (SML) cold-water coral (CWC) province (Northern Ionian Sea–Central Mediterranean Sea) was analyzed through the development of a mass-balanced ecosystem model. A total of nine balanced simulations were provided taking into account the biomass data collected during several experimental bottom trawl surveys carried out from 2005 to 2010 in a depth range between 101 and 541 m. A total of 100 species were included in the analysis corresponding to 15 cephalopods, 25 crustaceans, 7 chondrichthyes and 54 osteichthyes. Simulations were developed maintaining the species-level detail. The total system throughput estimated in the SML CWC province trophic web resulted mainly due to consumption and about 80% of it was maintained by species with trophic level between 2 and 4. Results highlighted important features in terms of the structure and functioning of the system, such as the high level of activity maintained by intermediate trophic levels and the important role of top-down control in the faunal assemblage due to both vertebrate and invertebrate species

Effects of Cell Culture Media on the Dynamic Formation of Protein−Nanoparticle Complexes and Influence on the Cellular Response

The development of appropriate in vitro protocols to assess the potential toxicity of the ever expanding range of nanoparticles represents a challenging issue, because of the rapid changes of their intrinsic physicochemical properties (size, shape, reactivity, surface area, etc.) upon dispersion in biological fluids. Dynamic formation of protein coating around nanoparticles is a key molecular event, which may strongly impact the biological response in nanotoxicological tests. In this work, by using citrate-capped gold nanoparticles (AuNPs) of different sizes as a model, we show, by several spectroscopic techniques (dynamic light scattering, UV−visible, plasmon resonance light scattering), that proteins−NP interactions are differently mediated by two widely used cellular media (i.e., Dulbecco Modified Eagle's medium (DMEM) and Roswell Park Memorial Institute medium (RPMI), supplemented with fetal bovine serum). We found that, while DMEM elicits the formation of a large time-dependent protein corona, RPMI s..