840 research outputs found

    Electromyographic Comparison Between the Rear-Foot-Elevated vs. B-Stance Unilateral Back Squat Techniques

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    The Rear-Foot-Elevated back squat (RFE) and B-Stance (BS) are two unilateral back squat techniques with the latter commonly proposed as the more stable of the two. There is currently a lack of research comparing these two techniques with respect to neuromuscular demand and movement characteristics. PURPOSE: The purpose of this study was to examine the muscle activation differences via surface electromyography (EMG) between the RFE vs. BS unilateral back squat techniques with equated load. METHODS: Thirteen healthy college-aged, resistance-trained male (n=10) and female (n=3) subjects were recruited for this study. Subjects visited the laboratory on two occasions separated by 5-7 days. Visit 1 included descriptive measurements and one-repetition max (1RM) testing for the RFE back squat. For visit 2, subjects performed the RFE and BS unilateral squat techniques under a load of 85% of RFE 1RM. Electromyographic assessment of the external oblique (OBL), rectus femoris (RF), gluteus maximums (GM) and biceps femoris (BF) was administered during each technique and subsequently compared. RESULTS: A paired sample t-test was used to compare mean and peak normalized root mean square (RMS) EMG between the RFE and BS techniques. There was no significant difference in mean and peak eccentric, concentric, and total activation between the RFE vs. BS for the OBL and RF. For GM, mean and peak eccentric, concentric, and total activation was greater during the RFE vs. BS (pCONCLUSION: The RFE unilateral back squat technique elicited overall greater activation of the hip extensors, GM and BF vs. BS. Based on these findings, the two unilateral squat techniques are not interchangeable from a muscle activation perspective which should be considered when employing unilateral back squat variations in training or rehabilitation programs

    Electromyographic Examination of Hip and Knee Extension Hex Bar Exercises Varied by Starting Knee and Torso Angles

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    International Journal of Exercise Science 15(1): 541-551, 2022. Variations of the deadlift can be executed using the hexagonal (hex) bar by altering, for instance, the knee and torso angles while maintaining a constant hip angle at the start position. PURPOSE: To examine muscle activation patterns of the biceps femoris, rectus femoris, and erector spinae during three deadlift variations using the hex bar. METHODS: Twenty resistance-trained male and female subjects performed hex bar deadlift variations in three different starting knee flexion positions: 128.4 ± 8.5°, 111.9 ± 8.7°, and 98.3 ± 6.5°. Subjects performed three repetitions at 75% of their three-repetition maximum. Electromyography sensors were placed on the dominant biceps femoris, rectus femoris, and lumbar erector spinae. A one-way repeated measures ANOVA was used to detect differences in mean and peak EMG values normalized to maximum voluntary isometric contraction (MVIC) (p \u3c 0.05). RESULTS: As knee flexion increased at the starting position, mean activation of the rectus femoris increased (24.7 ± 21.5 35.5 ± 25.4 62.1 ± 31.3% MVIC, p \u3c 0.001), while biceps femoris (40.6 ± 17.9 34.0 ± 16.4 28.1 ± 14.5% MVIC, p = 0.003) and erector spinae (73.0 ± 27.6 65.9 ± 34.4 54.9 ± 32.5% MVIC, p = 0.009) activation decreased. Peak activation of the rectus femoris increased (46.9 ± 33.0 60.9 ± 38.7 99.3 ± 41.6% MVIC, p \u3c 0.001) while decreasing in the erector spinae (118.6 ± 47.1 105.9 ± 49.4 89.1 ± 40.1% MVIC, p = 0.008). The rectus femoris experienced the greatest mean differences of the three muscles. CONCLUSIONS: Practitioners should consider the muscular goals when adjusting the starting position of a hex bar deadlift as posterior chain recruitment diminished and quadriceps activation increased as knee flexion increased

    Evaluation of the Civic Engagement Initiative, 2003-2004

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    The CEI integrated high levels of data collection, use, and analysis into its implementation model. TBF and members of the coordinating team also conducted evaluative and summary research at different periods throughout the project. To assess the CEI\u27s impact on voter participation, therefore, the evaluation team reviewed analyses by these stakeholders, including primarily data from the Massachusetts Voter Education Network (MassVOTE, January 2005), LeLievre Information Systems (March 2004; May 2005, ) and Northeast Action (June 2003); reports to the funders group by the donor collaborative liaison Bates Consulting (March 2004; various dates 2005); an evaluative report to TBF by the foundation\u27 s own research associate (2004); and assorted work plans and reports from the grantees. The team also assessed methodologies used in data collection. In certain cases, such as the review of the use of control precincts to assess project impact, the team analyzed raw data provided by the technical assistance providers together with neighborhood demographic information. To determine the nature and scope of additional products related to increased voter turnout, the team reviewed newspaper coverage of the CEI and voter participation in the target neighborhoods by consulting several indexes and examining media files of some of the grantees. Boston candidate lists were scrutinized for data on the number of candidates of color and females present in 2002-2005 contested preliminary elections

    The cytosolic carboxypeptidases CCP2 and CCP3 catalyze posttranslational removal of acidic amino acids

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    The posttranslational modification of carboxy-terminal tails of tubulin plays an important role in the regulation of the microtubule cytoskeleton. Enzymes responsible for deglutamylating tubulin have been discovered within a novel family of mammalian cytosolic carboxypeptidases. The discovery of these enzymes also revealed the existence of a range of other substrates that are enzymatically deglutamylated. Only four of six mammalian cytosolic carboxypeptidases had been enzymatically characterized. Here we complete the functional characterization of this protein family by demonstrating that CCP2 and CCP3 are deglutamylases, with CCP3 being able to hydrolyze aspartic acids with similar efficiency. Deaspartylation is a novel posttranslational modification that could, in conjunction with deglutamylation, broaden the range of potential substrates that undergo carboxy-terminal processing. In addition, we show that CCP2 and CCP3 are highly regulated proteins confined to ciliated tissues. The characterization of two novel enzymes for carboxy-terminal protein modification provides novel insights into the broadness of this barely studied process

    Genome analysis of the ubiquitous boxwood pathogen Pseudonectria foliicola

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    Boxwood (Buxus spp.) are broad-leaved, evergreen landscape plants valued for their longevity and ornamental qualities. Volutella leaf and stem blight, caused by the ascomycete fungi Pseudonectria foliicola and P. buxi, is one of the major diseases affecting the health and ornamental qualities of boxwood. Although this disease is less severe than boxwood blight caused by Calonectria pseudonaviculata and C. henricotiae, its widespread occurrence and disfiguring symptoms have caused substantial economic losses to the ornamental industry. In this study, we sequenced the genome of P. foliicola isolate ATCC13545 using Illumina technology and compared it to other publicly available fungal pathogen genomes to better understand the biology of this organism. A de novo assembly estimated the genome size of P. foliicola at 28.7 Mb (425 contigs; N50 = 184,987 bp; avg. coverage 188×), with just 9,272 protein-coding genes. To our knowledge, P. foliicola has the smallest known genome within the Nectriaceae. Consistent with the small size of the genome, the secretome, CAzyme and secondary metabolite profiles of this fungus are reduced relative to two other surveyed Nectriaceae fungal genomes: Dactylonectria macrodidyma JAC15-245 and Fusarium graminearum Ph-1. Interestingly, a large cohort of genes associated with reduced virulence and loss of pathogenicity was identified from the P. foliicola dataset. These data are consistent with the latest observations by plant pathologists that P. buxi and most likely P. foliicola, are opportunistic, latent pathogens that prey upon weak and stressed boxwood plants

    Physiological and behavioral effects of animal-assisted interventions for therapy dogs in pediatric oncology settings

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    Over the past two decades, animal-assisted interventions (AAIs), defined as the purposeful incorporation of specially trained animals in services to improve human health, have become increasingly popular in clinical settings. However, to date, there have been few rigorously-designed studies aimed at examining the impact of AAIs on therapy animals, despite a notable potential for stress. The current study measured physiological and behavioral stress indicators in therapy dogs who participated in AAI sessions in pediatric oncology settings, while also examining the psychosocial effects for patients and their parents. This manuscript describes the study’s canine stress findings. Methods: A total of 26 therapy dog-handler teams were paired with newly diagnosed children with cancer at five children’s hospitals in the United States. These teams provided regular AAI visits to the child and his/her parent(s) for a period of four months. The teams completed a demographic form, the Canine Behavioral Assessment & Research Questionnaire (C-BARQ), and a self-report survey to document the types of activities that occurred during each session. Canine saliva was also collected at five baseline time points and 20 minutes after the start of study sessions for cortisol analysis, and all study sessions were video recorded to document the dog’s behavior via an ethogram measure. Results: Data showed no significant differences in salivary cortisol levels between baseline (0.51µg/dL) and AAI sessions (0.44µg/dL), p = 0.757. Higher salivary cortisol was significantly associated with a higher number of stress behaviors per session (p = 0.039). There was a significant relationship between stress and affiliative session behaviors (pConclusions:Results show that therapy dogs did not have significantly increased physiological stress responses, nor did they exhibit significantly more stress-related behaviors than affiliative-related behaviors, while participating in AAIs in pediatric oncology settings. The significant relationship between canine cortisol and behavior, thus strengthening the argument for the use of cortisol in canine well-being research. This study discusses the importance of further investigation to confirm these findings, which may lead to enhanced canine involvement in hospital settings

    Randomized controlled trial comparing four strategies for delivering e-curriculum to health care professionals [ISRCTN88148532]

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    BACKGROUND: Internet education is increasingly provided to health professionals, but little is known about the most effective strategies for delivering the content. The purpose of this study is to compare four strategies for delivering an Internet-based (e-) curriculum on clinicians' knowledge (K), confidence (CONF), and communication (COMM) about herbs and other dietary supplements (HDS). METHODS: This national randomized 2 × 2 factorial trial included physicians, pharmacists, nurses, nutritionists and trainees in these fields. Participants were randomly assigned to one of four curriculum delivery strategies for 40 brief modules about HDS: a) delivering four (4) modules weekly over ten (10) weeks by email (drip-push); b) modules accessible on web site with 4 reminders weekly for 10 weeks (drip-pull); c) 40 modules delivered within 4 days by email (bolus-push); and d) 40 modules available on the Internet with one email informing participants of availability (bolus-pull). RESULTS: Of the 1,267 enrollees, 25% were male; the average age was 40 years. The completion rate was 62%, without significant differences between delivery groups. There were statistically significant improvements in K, CONF and COMM scores after the course (P<0.001 for all), although the difference in COMM was small. There were no significant differences in any of the three outcomes by delivery strategy, but outcomes were better for those who paid for continuing education credit. CONCLUSION: All delivery strategies tested similarly improved K, CONF, COMM scores about HDS. Educators can use the strategy that is most convenient without diminishing effectiveness. Additional curricula may be necessary to make substantial changes in clinicians' communication practices
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