PLASTIC CANNULA FOR RUMEN FISTULA

PLASTIC CANNULA FOR RUMEN FISTUL

Mejoramiento de diversos segmentos de red de área local dentro de un corporativo multinacional, para el correcto funcionamiento de transmisiones de video mediante protocolos multicast

Trabajo de obtención de grado que tuvo el objetivo de mejorar y actualizar varios segmentos de red de área local significativos para la empresa Intel. Se realizó un análisis apropiado del estado actual del desempeño de la red para servicios webcast y, para mejorar la experiencia del usuario en servicios de video, se aplicaron protocolos multicast

A Fungal Effector With Host Nuclear Localization and DNA-Binding Properties Is Required for Maize Anthracnose Development

Plant pathogens have the capacity to manipulate the host immune system through the secretion of effectors. We identified 27 putative effector proteins encoded in the genome of the maize anthracnose pathogen Colletotrichum graminicola that are likely to target the host’s nucleus, as they simultaneously contain sequence signatures for secretion and nuclear localization. We functionally characterized one protein, identified as CgEP1. This protein is synthesized during the early stages of disease development and is necessary for anthracnose development in maize leaves, stems, and roots. Genetic, molecular, and biochemical studies confirmed that this effector targets the host’s nucleus and defines a novel class of double-stranded DNA-binding protein. We show that CgEP1 arose from a gene duplication in an ancestor of a lineage of monocot-infecting Colletotrichum spp. and has undergone an intense evolution process, with evidence for episodes of positive selection. We detected CgEP1 homologs in several species of a grass-infecting lineage of Colletotrichum spp., suggesting that its function may be conserved across a large number of anthracnose pathogens. Our results demonstrate that effectors targeted to the host nucleus may be key elements for disease development and aid in the understanding of the genetic basis of anthracnose development in maize plants.Fil: Vargas, Walter Alberto. Universidad de Salamanca; España. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sanz Martín, José M.. Universidad de Salamanca; EspañaFil: Rech, Gabriel E.. Universidad de Salamanca; EspañaFil: Armijos Jaramillo, Vinicio D.. Universidad de Salamanca; EspañaFil: Rivera Rodriguez, Lina Patricia. Universidad de Salamanca; EspañaFil: Echeverria, María de Las Mercedes. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; ArgentinaFil: Díaz Mínguez, José M.. Universidad de Salamanca; EspañaFil: Thon, Michael R.. Universidad de Salamanca; EspañaFil: Sukno, Serenella A.. Universidad de Salamanca; Españ

Astrocyte reactivity to Fas activation is attenuated in TIMP-1 deficient mice, an in vitro study

BACKGROUND: Tissue inhibitor of metalloproteinases-1 (TIMP-1) is a multifunctional secreted protein with pleiotropic actions, including the inhibition of matrix metalloproteinases (MMPs), cell death/survival and growth promoting activities. After inflammatory challenge, the levels of TIMP-1 are highly and selectively upregulated in astrocytes among glial cells, but little is know about its role in these neural cells. We investigated the influence of TIMP-1 null mutation in the reactivity of cultured astrocytes to pro-inflammatory stimuli with TNF-α and anti-Fas antibody. RESULTS: When compared to WT, mutant astrocytes displayed an overall increased constitutive gelatinase expression and were less responsive to Fas-mediated upregulation of MMP-9, of monocyte chemoattractant protein-1 (MCP-1) and of intercellular cell adhesion molecule-1 (ICAM-1), all markers of astrocyte inflammatory response. In contrast, TNF-α treatment induced all these factors similarly regardless of the astrocyte genotype. The incorporation of (3)H-thymidin, a marker of cell proliferation, increased in wild-type (WT) astrocytes after treatment with anti-Fas antibody or recombinant TIMP-1 but not in mutant astrocytes. Finally, lymphocyte chemotaxis was differentially regulated by TNF-α in WT and TIMP-1 deficient astrocytes. CONCLUSION: We provide evidence that the alteration of the MMP/TIMP balance in astrocytes influences their reactivity to pro-inflammatory stimuli and that Fas activation modulates the expression of members of the MMP/TIMP axis. We hypothesise that the Fas/FasL transduction pathway and the MMP/TIMP system interact in astrocytes to modulate their inflammatory response to environmental stimuli

Comunidad de Escarabajos Coprófagos (Coleoptera: Scarabaeidae: Scarabaeinae) en Hábitats bajo Distinta Intensidad de Uso en Yuscarán, Honduras

The Municipality of Yuscarán is under continuous habitat degradation processes due to invasive agriculture and cattle practices. Dung beetles (Coleoptera: Scarabaeidae: Scarabaeinae) are sensitive to habitat disturbance. The objective of this study was to describe how the dung beetle community structure is modified from less to more perturbed habitats throughout three different landscapes, according to the intensity of land use and vegetation structure. The investigation took place from June to December 2012 in a Tropical Evergreen Pine Forest and in a Deciduous Woodland. Each forest type consisted of two different areas divided by 3 collection sites according to land use. Beetles were collected in pitfall traps baited with cow dung. Vegetation structure and abiotic parameters were determined per habitat. A total of 2,749 beetles were captured, belonging to 9 tribes, 11 genera, and 21 species. Mean beetle abundance was reduced by 50% due to increased human intervention. In the Evergreen Forest, the dung beetle community structure was mainly formed by forest specialists and generalists, whereas in the Deciduous Woodland the dung beetle community was formed mainly by generalist and heliophilic species. The study shows how human intervention diminishes the richness, diversity, and abundance of the dung beetle communities in the Evergreen Forest. However, there is an inverse scenario in the Deciduous Woodland where the results indicated that there could be a faunal homogenization of generalist beetles, biased towards degraded and highly perturbed habitats. DOI: http://dx.doi.org/10.5377/ceiba.v52i2.1758Los recursos naturales en el Municipio de Yuscarán se encuentran bajo un continuo proceso de degradación producto de mal manejo del suelo, así como prácticas agrícolas y ganaderas intensivas. Los escarabajos coprófagos (Coleoptera: Scarabaeidae: Scarabaeinae) son insectos muy susceptibles a la perturbación del hábitat; por lo tanto, el objetivo de la investigación fue describir como la estructura de sus comunidades es modificada desde hábitats menos a más intervenidos, a través de diferentes usos del suelo, tomando en cuenta la intensidad de uso y su estructura vegetal. La investigación se realizó de junio a diciembre del 2012 en un Bosque Tropical Siempre Verde y un Arbustal Deciduo de Tierras Bajas, cada uno con dos zonas divididas en tres sitios de muestreo según el uso del suelo. Los escarabajos se recolectaron utilizando trampas de caída cebadas con estiércol de vaca. Se caracterizó la vegetación y se registraron parámetros abióticos por hábitat. Se capturaron 2,749 individuos pertenecientes a 9 tribus, 11 géneros y 21 especies. La abundancia promedio de escarabajos disminuyó más del 50% al intensificarse la intervención humana. En el Bosque Siempre Verde, las comunidades de escarabajos estuvieron conformadas principalmente por especialistas y generalistas forestales; en el Arbustal Deciduo, las comunidades estaban compuestas por especies generalistas y heliofílicas. Este estudio muestra como la intervención humana conlleva a la pérdida de riqueza, diversidad y abundancia de las comunidades de escarabajos del Bosque Siempre Verde. No obstante, la situación es inversa y más precaria en el Ecosistema Deciduo, donde los resultados indican que se puede estar llegando a una homogenización de fauna con hábitos generalistas producto del alto grado de intervención del hábitat.DOI: http://dx.doi.org/10.5377/ceiba.v52i2.175

EFFECT OF ESTROGEN IN THE ELIMINATION OF SEXUAL ODOR IN BOARS

EFFECT OF ESTROGEN IN THE ELIMINATION OF SEXUAL ODOR IN BOAR

Computer modeling of an impedance-controlled pulsing protocol for RF tumor ablation with a cooled electrode

[EN] Purpose: To develop computer models to mimic the impedance-controlled pulsing protocol implemented in radiofrequency (RF) generators used for clinical practice of radiofrequency ablation (RFA), and to assess the appropriateness of the models by comparing the computer results with those obtained in previous experimental studies.Methods: A 12-min RFA was modelled using a cooled electrode (17G, 3cm tip) inserted in hepatic tissue. The short (transverse) diameter of the coagulation zone was assessed under in vivo (with blood perfusion (BP) and considering clamping) and ex vivo (at 21 degrees C) conditions. The computer results obtained by programming voltage pulses were compared with current pulses.Results: The differences between voltage and current pulses were noticeable: using current instead of voltage allows larger coagulation zones to be created, due to the higher energy applied by current pulses. If voltage pulses are employed the model can accurately predict the number of roll-offs, although the waveform of the applied power is clearly not realistic. If current voltages are employed, the applied power waveform matches well with those reported experimentally, but there are significantly fewer roll-offs. Our computer results were overall into the ranges of experimental ones.Conclusions: The proposed models reproduce reasonably well the electrical-thermal performance and coagulation zone size obtained during an impedance-controlled pulsing protocol.This work was supported by the Spanish Plan Estatal de Investigacion, Desarrollo e Innovacion Orientada a los Retos de la Sociedad under grant number TEC2014-52383-C3-R (TEC2014-52383-C3-1-R). The authors alone are responsible for the content and writing of the paper.Trujillo Guillen, M.; Bon Corbín, J.; Rivera Ortun, MJ.; Burdio, F.; Berjano, E. (2016). Computer modeling of an impedance-controlled pulsing protocol for RF tumor ablation with a cooled electrode. International Journal of Hyperthermia. 32(8):931-939. doi:10.1080/02656736.2016.1190868S931939328Hocquelet, A., Balageas, P., Laurent, C., Blanc, J.-F., Frulio, N., Salut, C., … Trillaud, H. (2015). Radiofrequency ablation versus surgical resection for hepatocellular carcinoma within the Milan criteria: A study of 281 Western patients. International Journal of Hyperthermia, 31(7), 749-757. doi:10.3109/02656736.2015.1068382Fukushima, T., Ikeda, K., Kawamura, Y., Sorin, Y., Hosaka, T., Kobayashi, M., … Kumada, H. (2015). Randomized Controlled Trial Comparing the Efficacy of Impedance Control and Temperature Control of Radiofrequency Interstitial Thermal Ablation for Treating Small Hepatocellular Carcinoma. Oncology, 89(1), 47-52. doi:10.1159/000375166Goldberg, S. N., Stein, M. C., Gazelle, G. S., Sheiman, R. G., Kruskal, J. B., & Clouse, M. E. (1999). Percutaneous Radiofrequency Tissue Ablation: Optimization of Pulsed-Radiofrequency Technique to Increase Coagulation Necrosis. Journal of Vascular and Interventional Radiology, 10(7), 907-916. doi:10.1016/s1051-0443(99)70136-3Ahmed, M., Liu, Z., Humphries, S., & Nahum Goldberg, S. (2008). Computer modeling of the combined effects of perfusion, electrical conductivity, and thermal conductivity on tissue heating patterns in radiofrequency tumor ablation. International Journal of Hyperthermia, 24(7), 577-588. doi:10.1080/02656730802192661Lobo, S. M., Liu, Z.-J., Yu, N. C., Humphries, S., Ahmed, M., Cosman, E. R., … Goldberg, S. N. (2005). RF tumour ablation: Computer simulation and mathematical modelling of the effects of electrical and thermal conductivity. International Journal of Hyperthermia, 21(3), 199-213. doi:10.1080/02656730400001108Solazzo, S. A., Liu, Z., Lobo, S. M., Ahmed, M., Hines-Peralta, A. U., Lenkinski, R. E., & Goldberg, S. N. (2005). Radiofrequency Ablation: Importance of Background Tissue Electrical Conductivity—An Agar Phantom and Computer Modeling Study. Radiology, 236(2), 495-502. doi:10.1148/radiol.2362040965Barauskas, R., Gulbinas, A., & Barauskas, G. (2007). Investigation of radiofrequency ablation process in liver tissue by finite element modeling and experiment. Medicina, 43(4), 310. doi:10.3390/medicina43040039Haemmerich, D., & Wood, B. J. (2006). Hepatic radiofrequency ablation at low frequencies preferentially heats tumour tissue. International Journal of Hyperthermia, 22(7), 563-574. doi:10.1080/02656730601024727Haemmerich, D., Chachati, L., Wright, A. S., Mahvi, D. M., Lee, F. T., & Webster, J. G. (2003). Hepatic radiofrequency ablation with internally cooled probes: effect of coolant temperature on lesion size. IEEE Transactions on Biomedical Engineering, 50(4), 493-500. doi:10.1109/tbme.2003.809488Schutt, D. J., & Haemmerich, D. (2008). Effects of variation in perfusion rates and of perfusion models in computational models of radio frequency tumor ablation. Medical Physics, 35(8), 3462-3470. doi:10.1118/1.2948388Zhang, B., Moser, M. A. J., Zhang, E. M., Luo, Y., & Zhang, W. (2015). Numerical analysis of the relationship between the area of target tissue necrosis and the size of target tissue in liver tumours with pulsed radiofrequency ablation. International Journal of Hyperthermia, 31(7), 715-725. doi:10.3109/02656736.2015.1058429Solazzo, S. A., Ahmed, M., Liu, Z., Hines-Peralta, A. U., & Goldberg, S. N. (2007). High-Power Generator for Radiofrequency Ablation: Larger Electrodes and Pulsing Algorithms in Bovine ex Vivo and Porcine in Vivo Settings. Radiology, 242(3), 743-750. doi:10.1148/radiol.2423052039Abraham, J. P., & Sparrow, E. M. (2007). A thermal-ablation bioheat model including liquid-to-vapor phase change, pressure- and necrosis-dependent perfusion, and moisture-dependent properties. International Journal of Heat and Mass Transfer, 50(13-14), 2537-2544. doi:10.1016/j.ijheatmasstransfer.2006.11.045Pätz, T., Kröger, T., & Preusser, T. (2009). Simulation of Radiofrequency Ablation Including Water Evaporation. World Congress on Medical Physics and Biomedical Engineering, September 7 - 12, 2009, Munich, Germany, 1287-1290. doi:10.1007/978-3-642-03882-2_341Trujillo, M., Alba, J., & Berjano, E. (2012). Relationship between roll-off occurrence and spatial distribution of dehydrated tissue during RF ablation with cooled electrodes. International Journal of Hyperthermia, 28(1), 62-68. doi:10.3109/02656736.2011.631076Hall, S. K., Ooi, E. H., & Payne, S. J. (2015). Cell death, perfusion and electrical parameters are critical in models of hepatic radiofrequency ablation. International Journal of Hyperthermia, 31(5), 538-550. doi:10.3109/02656736.2015.1032370Chang, S.-J., Yu, W.-J., Chang, C.-C., & Chen, Y.-H. (2010). 7 PROTEOMICS ANALYSIS OF MALE REPRODUCTIVE PHYSIOLOGY BY TOONA SINENSIS ROEM. Reproductive BioMedicine Online, 20, S3-S4. doi:10.1016/s1472-6483(10)62425-xBeop-Min Kim, Jacques, S. L., Rastegar, S., Thomsen, S., & Motamedi, M. (1996). Nonlinear finite-element analysis of the role of dynamic changes in blood perfusion and optical properties in laser coagulation of tissue. IEEE Journal of Selected Topics in Quantum Electronics, 2(4), 922-933. doi:10.1109/2944.577317Doss, J. D. (1982). Calculation of electric fields in conductive media. Medical Physics, 9(4), 566-573. doi:10.1118/1.595107Jo, B., & Aksan, A. (2010). Prediction of the extent of thermal damage in the cornea during conductive keratoplasty. Journal of Thermal Biology, 35(4), 167-174. doi:10.1016/j.jtherbio.2010.02.004Belous, A., & Podhajsky, R. J. (2009). The effect of initial and dynamic liver conditions on RF ablation size: a study in perfused and non-perfused animal models. Energy-based Treatment of Tissue and Assessment V. doi:10.1117/12.809597Song, K. D., Lee, M. W., Park, H. J., Cha, D. I., Kang, T. W., Lee, J., … Rhim, H. (2015). Hepatic radiofrequency ablation:in vivoandex vivocomparisons of 15-gauge (G) and 17-G internally cooled electrodes. The British Journal of Radiology, 88(1050), 20140497. doi:10.1259/bjr.20140497Cha, J., Choi, D., Lee, M. W., Rhim, H., Kim, Y., Lim, H. K., … Park, C. K. (2009). Radiofrequency Ablation Zones in Ex Vivo Bovine and In Vivo Porcine Livers: Comparison of the Use of Internally Cooled Electrodes and Internally Cooled Wet Electrodes. CardioVascular and Interventional Radiology, 32(6), 1235-1240. doi:10.1007/s00270-009-9600-0Lee, J. M., Han, J. K., Chang, J. M., Chung, S. Y., Kim, S. H., Lee, J. Y., … Choi, B. I. (2006). Radiofrequency Ablation of the Porcine Liver In Vivo: Increased Coagulation with an Internally Cooled Perfusion Electrode. 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Medicina humana espacial: Performance fisiológico y contramedidas para mejorar la salud del astronauta: Human space medicine: Physiological performance and countermeasures to improve the astronaut's health

This Review Article is presented based on current scientific evidence on space medicine focused on human physiology and its countermeasures. Therefore, a non-systematic bibliographic search of scientific articles and research books in English-Spanish of the last 7 years was carried out, detailing their application in humans, murine models and in vitro experiments. The conditions of the space environment such as microgravity and radiation that produce considerable physiological changes in the cardiovascular system (redistribution of fluids, cardiovascular remodeling, arrhythmias) were taken into account; nervous (sensorimotor, neurosensory, neurovestibular); respiratory (volume and capacity changes); renal (lithiasis); musculoskeletal (muscular atrophy, osteoporosis); hematological (anemia); immunological (immune dysregulation) and digestive (intestinal microbiota disorder). In addition, there are biological, molecular and genetic processes still to be explored, in order to know and mitigate the uncertain mechanisms triggered in extreme and dangerous environments. Therefore, it is a priority to develop and implement countermeasures to reduce the harmful effects on health, with the aim of guaranteeing the astronaut's adaptation, safety and performance during future space flights.Se presenta este Artículo de Revisión con base en la evidencia científica actual sobre medicina espacial enfocada en fisiología humana y sus contramedidas. Por lo cual se realizó una búsqueda bibliográfica no sistemática de artículos científicos y libros de investigación en inglés-español de los últimos 7 años, que detallan su aplicación en seres humanos, modelos murinos y experimentos in vitro. Se tomaron en cuenta las condiciones del ambiente espacial como microgravedad y radiación que producen considerables cambios fisiológicos en el sistema cardiovascular (redistribución de líquidos, remodelación cardiovascular, arritmias); nervioso (sensitivomotores, neurosensoriales, neurovestibulares); respiratorio (cambios de volúmenes y capacidades); renal (litiasis); musculo-esquelético (atrofia muscular, osteoporosis); hematológico (anemia); inmunológico (desregulación inmune) y digestivo (alteración de la microbiota intestinal). Además, existen procesos biológicos, moleculares y genéticos aún por explorar, para conocer y mitigar los mecanismos inciertos desencadenados en ambientes extremos y peligrosos. Por lo tanto, es una prioridad desarrollar e implementar contramedidas para reducir los efectos nocivos en la salud, con el objetivo de garantizar la adaptación, seguridad y performance del astronauta durante futuros viajes espaciales

Dung beetles (Coleoptera: Scarabaeidae) in grazing lands of the Neotropics: A review of patterns and research trends of taxonomic and functional diversity, and functions

Dung beetles are one of the most representative groups of insects associated with livestock, as they take advantage of the manure of livestock for food and reproduction. They have been widely used as a bio-indicator group to evaluate their responses to land-use change and other environmental disturbances by analyzing species diversity at different spatial and temporal scales. However, the impacts of livestock management practices, forms, and history on dung beetle diversity are still poorly understood. This paper is an exhaustive and systematic review of the existing peer-reviewed and indexed literature on the taxonomic diversity (species richness and composition), functional diversity, and ecological functions of dung beetles from different provinces and biogeographic domains in tropical grazing lands of the Neotropics. We analyzed the timeline of the studies conducted so far, and we detected increasing literature produced mainly in South America. We included the most frequent objectives, tendencies, software, and statistical analyses. Given the significant heterogeneity of livestock landscapes in the Neotropics, the reviewed studies have conceptualized broadly what a pasture is, and the authors have used different descriptions of other grazing lands. Additionally, management data and livestock practices vary widely among studies, and management history is poorly described. In future research, it is relevant to include as much information as possible and the consequences of different livestock management practices on additional ecological attributes of dung beetle assemblages at different landscape scales (spatial and temporal) to predict how ecological processes change in Neotropical landscapes. Considering the importance of the data to be included in future work, we propose a collection format to unify the information collected in the field when conducting ecological studies in grazing lands. Moreover, we define a classification system to homogenize the features that distinguish the multiple tropical grazing lands reported in the literature. Preserving dung beetle diversity and associated ecological functions is urgent for maintaining ecosystem services in grazing lands. The challenge for research institutions is to continue filling gaps in knowledge to help those who work in transferring knowledge, to help ranchers exercise better options for more sustainable livestock farming, and to publish results for conservation decision-making

IFE Plant Technology Overview and contribution to HiPER proposal

HiPER is the European Project for Laser Fusion that has been able to join 26 institutions and signed under formal government agreement by 6 countries inside the ESFRI Program of the European Union (EU). The project is already extended by EU for two years more (until 2013) after its first preparatory phase from 2008. A large work has been developed in different areas to arrive to a design of repetitive operation of Laser Fusion Reactor, and decisions are envisioned in the next phase of Technology Development or Risk Reduction for Engineering or Power Plant facilities (or both). Chamber design has been very much completed for Engineering phase and starting of preliminary options for Reactor Power Plant have been established and review here