1,449 research outputs found
Quantum resistance metrology in graphene
We have performed a metrological characterization of the quantum Hall
resistance in a 1 m wide graphene Hall-bar. The longitudinal resistivity
in the center of the quantum Hall plateaus vanishes within the
measurement noise of 20 m upto 2 A. Our results show that the
quantization of these plateaus is within the experimental uncertainty (15 ppm
for 1.5A current) equal to that in conventional semiconductors. The
principal limitation of the present experiments are the relatively high contact
resistances in the quantum Hall regime, leading to a significantly increased
noise across the voltage contacts and a heating of the sample when a high
current is applied
Import of cytochrome c into mitochondria
The import of cytochrome c into mitochondria can be resolved into a number of discrete steps. Here we report on the covalent attachment of heme to apocytochrome c by the enzyme cytochrome c heme lyase in mitochondria from Neurospora crassa.
A new method was developed to measure directly the linkage of heme to apocytochrome c. This method is independent of conformational changes in the protein accompanying heme attachment. Tryptic peptides of [35S]cysteine-labelled apocytochrome c, and of enzymatically formed holocytochrome c, were resolved by reverse-phase HPLC. The cysteine-containing peptide to which heme was attached eluted later than the corresponding peptide from apocytochrome c and could be quantified by counting 35S radioactivity as a measure of holocytochrome c formation. Using this procedure, the covalent attachment of heme to apocytochrome c, which is dependent on the enzyme cytochrome c heme lyase, could be measured. Activity required heme (as hemin) and could be reversibly inhibited by the analogue deuterohemin. Holocytochrome c formation was stimulated 5–10-fold by NADH > NADPH > glutathione and was independent of a potential across the inner mitochondrial membrane. NADH was not required for the binding of apocytochrome c to mitochondria and was not involved in the reduction of the cysteine thiols prior to heme attachment. Holocytochrome c formation was also dependent on a cytosolic factor that was necessary for the heme attaching step of cytochrome c import. The factor was a heat-stable, protease-insensitive, low-molecular-mass component of unknown function.
Cytochrome c heme lyase appeared to be a soluble protein located in the mitochondrial intermembrane space and was distinct from the previously identified apocytochrome c binding protein having a similar location. A model is presented in which the covalent attachment of heme by cytochrome c heme lyase also plays an essential role in the import pathway of cytochrome c
Immobilized photocatalyst on stainless steel woven meshes assuring efficient light distribution in a solar reactor
An immobilized TiO<sub>2</sub> photocatalyst with a high specific surface area was
prepared on stainless steel woven meshes in order to be used packed in layers
for water purification. Immobilization of such a complex shape needs a
special coating technique. For this purpose, dip coating and electrophoretic
deposition (EPD) techniques were used. The EPD technique gave the TiO<sub>2</sub>
coating films a better homogeneity and adhesion, fewer cracks, and a higher
<sup>·</sup>OH formation than the dip coating technique. The woven mesh
structure packed in layers guaranteed an efficient light-penetration in water
treatment reactor. A simple equation model was used to describe the
distribution of light through the mesh layers in the presence of absorbing
medium (e.g., colored water with humic acids). Maximum three or four coated
meshes were enough to harvest the solar UV light from 300 nm to 400 nm with
a high penetration efficiency. The separation distance between the mesh
layers played an important role in the efficiency of solar light penetration
through the coated mesh layers, especially in case of colored water
contaminated with high concentrations of humic acid
Accurate measurements of small currents using a CCC with DC SQUID read out
The continuous miniaturisation in the semiconductor industry increases the importance of accurate measurement and control of very small currents. In the field of precision electrical measurements, cryogenic current comparators (CCCs) are used as extremely sensitive and accurate instruments for scaling currents. In an international co-operation project, a special CCC system is being developed, optimised for the measurement of extremely small currents to less than 1 pA (10−12 A). DC superconducting quantum interference device (SQUID) readout is used for monitoring the ampere-turn unbalance of the CCC. In this paper, we report on the accurate determination of the CCC and SQUID input coil inductances. Matching of these inductances is required for obtaining ultimate current resolution with the CCC. We find very good agreement between measurements of the CCC inductance at room temperature and at 4.2 K, and results from numerical calculations. The measured values for the input and mutual inductance of the SQUID sensor are in good agreement with the design values when the effect of the slit in the SQUID washer is correctly taken into account. Final current resolution of our measurement system is expected to be better than 1×10−15 A/√Hz for a CCC with 20,000 primary windings
Phenomena induced by powerful HF pumping towards magnetic zenith with a frequency near the F-region critical frequency and the third electron gyro harmonic frequency
Multi-instrument observational data from an experiment on 13 October 2006 at the EISCAT/HEATING facility at Tromsø, Norway are analysed. The experiment was carried out in the evening hours when the electron density in the F-region dropped, and the HF pump frequency <I>f<sub>H</sub></I> was near and then above the critical frequency of the F2 layer. The distinctive feature of this experiment is that the pump frequency was just below the third electron gyro harmonic frequency, while both the HF pump beam and UHF radar beam were directed towards the magnetic zenith (MZ). The HF pump-induced phenomena were diagnosed with several instruments: the bi-static HF radio scatter on the London-Tromsø-St. Petersburg path, the CUTLASS radar in Hankasalmi (Finland), the European Incoherent Scatter (EISCAT) UHF radar at Tromsø and the Tromsø ionosonde (dynasonde). The results show thermal electron excitation of the HF-induced striations seen simultaneously from HF bi-static scatter and CUTLASS radar observations, accompanied by increases of electron temperature when the heater frequency was near and then above the critical frequency of the F2 layer by up to 0.4 MHz. An increase of the electron density up to 25% accompanied by strong HF-induced electron heating was observed, only when the heater frequency was near the critical frequency and just below the third electron gyro harmonic frequency. It is concluded that the combined effect of upper hybrid resonance and gyro resonance at the same altitude gives rise to strong electron heating, the excitation of striations, HF ray trapping and extension of HF waves to altitudes where they can excite Langmuir turbulence and fluxes of electrons accelerated to energies that produce ionization
A predictive model for swallowing dysfunction after curative radiotherapy in head and neck cancer
First modulation of high-frequency polar mesospheric summer echoes by radio heating of the ionosphere
The first high-frequency (HF, 8 MHz) observations of the modulation of polar mesospheric summer echoes (PMSE) by artificial radio heating of the ionosphere are presented and compared to observations at 224 MHz and model predictions. The experiments were performed at the European Incoherent Scatter facility in northern Norway. It is shown that model results are in qualitative and partial quantitative agreement with the observations, supporting the prediction that with certain ranges of ice particle radii and concentration, PMSE at HF radar wavelengths can be enhanced by heating due to the dominance of dust charging over plasma diffusion
Routine screening for Coxiella burnetii infection during pregnancy:a clustered randomised controlled trial during an outbreak, the Netherlands, 2010
Ice XII in its second regime of metastability
We present neutron powder diffraction results which give unambiguous evidence
for the formation of the recently identified new crystalline ice phase[Lobban
et al.,Nature, 391, 268, (1998)], labeled ice XII, at completely different
conditions. Ice XII is produced here by compressing hexagonal ice I_h at T =
77, 100, 140 and 160 K up to 1.8 GPa. It can be maintained at ambient pressure
in the temperature range 1.5 < T < 135 K. High resolution diffraction is
carried out at T = 1.5 K and ambient pressure on ice XII and accurate
structural properties are obtained from Rietveld refinement. At T = 140 and 160
K additionally ice III/IX is formed. The increasing amount of ice III/IX with
increasing temperature gives an upper limit of T ~ 150 K for the successful
formation of ice XII with the presented procedure.Comment: 3 Pages of RevTeX, 3 tables, 3 figures (submitted to Physical Review
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