An Explanation Oriented Dialogue Approach and Its Application to Wicked Planning Problems

In this paper we consider an interactive and explanation based dialogue approach to complex and `wicked' planning problems. Wicked problems are essentially imprecisely formulated problems having no clearly defined goals and constraints. The dialogue approach is aimed at reducing the problem complexity during interaction with the human expert. The involved software agents are mostly optimization procedures. The approach contains the following steps: (1) Selection of a specific concern in a proposed solution; (2) Calculation of a stakeholder defined ideal plan; and (3) Comparing the actually generated plan and the prototype based on a similarity measure. The comparison of the actual and the ideal plan looks at aspects of interest for the stakeholder such as resource consumptions or structural properties of the plan. The proposed approach is generic and was applied and customized to three classes of wicked problems: release planning, investment planning, and urban planning. All three applications are described and illustrated in the paper

Transient Thrombasthenia in a Patient with Tuberculosis

A 20-year-old Black female presented with menorrhagia and epistaxis. Similar episodes, two years previously, had necessitated blood transfusion. No association with drug ingestion was found and the family history revealed no bleeding diathesis. The patient had a normal platelet count and a prolonged bleeding time. Platelet aggregation, clot retraction and platelet factor 3 release were grossly abnormal. She had axillary tuberculous lymphadenitis and chemotherapy was initiated. Three months later there was no clinical or laboratory evidence of a platelet abnormality. No reason for this transient thrombasthenia, which is unknown to us, was found. Glanzmann's disease is a hereditary thrombasthenia with a similar qualitative platelet defect

Theory of Shubnikov--De Haas Oscillations Around the ν=1/2\nu=1/2 Filling Factor of the Landau Level: Effect of Gauge Field Fluctuations

We present a theory of magnetooscillations around the $\nu =1/2$ Landau level filling factor based on a model with a fluctuating Chern--Simons field. The quasiclassical treatment of the problem is appropriate and leads to an unconventional $\exp\left[-(\pi/\omega_c\tau^*_{1/2})^4\right]$ behavior of the amplitude of oscillations. This result is in good qualitative agreement with available experimental data.Comment: Revtex, 4 pages, 1 figure attached as PostScript fil

Dystonia and paroxysmal dyskinesias: under-recognized movement disorders in domestic animals? A comparison with human dystonia/paroxysmal dyskinesias.

Dystonia is defined as a neurological syndrome characterized by involuntary sustained or intermittent muscle contractions causing twisting, often repetitive movements, and postures. Paroxysmal dyskinesias are episodic movement disorders encompassing dystonia, chorea, athetosis, and ballism in conscious individuals. Several decades of research have enhanced the understanding of the etiology of human dystonia and dyskinesias that are associated with dystonia, but the pathophysiology remains largely unknown. The spontaneous occurrence of hereditary dystonia and paroxysmal dyskinesia is well documented in rodents used as animal models in basic dystonia research. Several hyperkinetic movement disorders, described in dogs, horses and cattle, show similarities to these human movement disorders. Although dystonia is regarded as the third most common movement disorder in humans, it is often misdiagnosed because of the heterogeneity of etiology and clinical presentation. Since these conditions are poorly known in veterinary practice, their prevalence may be underestimated in veterinary medicine. In order to attract attention to these movement disorders, i.e., dystonia and paroxysmal dyskinesias associated with dystonia, and to enhance interest in translational research, this review gives a brief overview of the current literature regarding dystonia/paroxysmal dyskinesia in humans and summarizes similar hereditary movement disorders reported in domestic animals

The quantum J_{1}-J_{1'}-J_{2} spin-1/2 Heisenberg antiferromagnet: A variational method study

The phase transition of the quantum spin-1/2 frustrated Heisenberg antiferroferromagnet on an anisotropic square lattice is studied by using a variational treatment. The model is described by the Heisenberg Hamiltonian with two antiferromagnetic interactions: nearest-neighbor (NN) with different coupling strengths J_{1} and J_{1'} along x and y directions competing with a next-nearest-neighbor coupling J_{2} (NNN). The ground state phase diagram in the ({\lambda},{\alpha}) space, where {\lambda}=J_{1'}/J_{1} and {\alpha}=J_{2}/J_{1}, is obtained. Depending on the values of {\lambda} and {\alpha}, we obtain three different states: antiferromagnetic (AF), collinear antiferromagnetic (CAF) and quantum paramagnetic (QP). For an intermediate region {\lambda}_{1}<{\lambda}<1 we observe a QP state between the ordered AF and CAF phases, which disappears for {\lambda} above some critical value {\lambda}_{1}. The boundaries between these ordered phases merge at the quantum critical endpoint (QCE). Below this QCE there is again a direct first-order transition between the AF and CAF phases, with a behavior approximately described by the classical line {\alpha}_{c}{\simeq}{\lambda}/2

Tuning Anti-Klein to Klein Tunneling in Bilayer Graphene

We show that in gapped bilayer graphene, quasiparticle tunneling and the corresponding Berry phase can be controlled such that they exhibit features of single-layer graphene such as Klein tunneling. The Berry phase is detected by a high-quality Fabry-Pérot interferometer based on bilayer graphene. By raising the Fermi energy of the charge carriers, we find that the Berry phase can be continuously tuned from 2π down to 0.68π in gapped bilayer graphene, in contrast to the constant Berry phase of 2π in pristine bilayer graphene. Particularly, we observe a Berry phase of π, the standard value for single-layer graphene. As the Berry phase decreases, the corresponding transmission probability of charge carriers at normal incidence clearly demonstrates a transition from anti-Klein tunneling to nearly perfect Klein tunneling

High prevalence of multidrug resistant Escherichia coli isolated from fresh vegetables sold by selected formal and informal traders in the most densely populated Province of South Africa

Contaminated fresh produce has increasingly been implicated in foodborne disease outbreaks. As microbiological safety surveillance in South Africa is limited, a total of 545 vegetable samples (spinach, tomato, lettuce, cucumber, and green beans) were purchased from retailers, street traders, trolley vendors and farmers' markets. Escherichia coli, coliforms and Enterobacteriaceae were enumerated and the prevalence of Escherichia coli, Salmonella spp. and Listeria monocytogenes determined. E. coli isolates were characterized phenotypically (antibiotic resistance) and genotypically (diarrheagenic virulence genes). Coliforms, E. coli and Enterobacteriaceae counts were mostly not significantly different between formal and informal markets, with exceptions noted on occasion. When compared to international standards, 90% to 98% tomatoes, 70% to 94% spinach, 82% cucumbers, 93% lettuce, and 80% green bean samples, had satisfactory (≤ 100 CFU/g) E. coli counts. Of the 545 vegetable samples analyzed, 14.86% (n = 81) harbored E. coli, predominantly from leafy green vegetables. Virulence genes (lt, st, bfpA, eagg, eaeA, stx1, stx2, and ipaH) were not detected in the E. coli isolates (n = 67) characterized, however 40.30% were multidrug-resistant. Resistance to aminoglycosides (neomycin, 73.13%; gentamycin, < 10%), penicillins (ampicillin, 38.81%; amoxicillin, 41.79%; augmentin, < 10%), sulfonamides (cotrimoxazole, 22.39%), tetracycline (19.4%), chloramphenicol (11.94%), cephalosporins (cefepime, 34.33%), and carbapenemases (imipenem, < 10%) were observed. This study highlights the need for continued surveillance of multidrug resistant foodborne pathogens in fresh produce retailed formally and informally for potential consumer health risks. PRACTICAL APPLICATION: The results indicate that the microbiological quality of different vegetables were similar per product type, regardless of being purchased from formal retailers or informal street traders, trolley vendors or farmers' markets. Although no pathogenic bacteria (diarrheagenic E. coli, Salmonella spp. or L. monocytogenes) were isolated, high levels of multidrug-resistance was observed in the generic E. coli isolates. These findings highlight the importance of microbiological quality surveillance of fresh produce in formal and informal markets, as these products can be a reservoir of multidrug resistant bacteria harboring antibiotic resistance and virulence genes, potentially impacting human health

Dephasing time of composite fermions

We study the dephasing of fermions interacting with a fluctuating transverse gauge field. The divergence of the imaginary part of the fermion self energy at finite temperatures is shown to result from a breakdown of Fermi's golden rule due to a faster than exponential decay in time. The strong dephasing affects experiments where phase coherence is probed. This result is used to describe the suppression of Shubnikov-de Haas (SdH) oscillations of composite fermions (oscillations in the conductivity near the half-filled Landau level). We find that it is important to take into account both the effect of dephasing and the mass renormalization. We conclude that while it is possible to use the conventional theory to extract an effective mass from the temperature dependence of the SdH oscillations, the resulting effective mass differs from the $m^\ast$ of the quasiparticle in Fermi liquid theory.Comment: 14 pages, RevTeX 3.0, epsf, 1 EPS figur

Microbiological safety of spinach throughout commercial supply chains in Gauteng Province, South Africa and characterisation of isolated multidrug resistant Escherichia coli

Aim: To investigate the microbiological quality, potential human foodborne pathogen presence, and to phenotypically (antimicrobial resistance profiles) and genotypically (DNA fingerprinting and diarrheagenic gene presence) characterise Escherichia coli isolated throughout commercial spinach production systems from farm-to-sale. Methods and Results: Samples (n=288) were collected from two commercial supply chains using either river or borehole water for irrigation. Escherichia coli was enumerated throughout the chain where river water was directly used for overhead irrigation at levels between 0.00-3.22 log CFU.g-1. Mean Enterobacteriaceae and coliform counts of spinach ranged between 3.33-6.57 log CFU.g-1 and 3.33-6.64 log CFU.g-1, respectively. Following enrichment, isolation and MALDI-TOF identification, E. coli was isolated from 22.57% (n=65/288) of all samples, Salmonella spp. from 3% (n=9/288) of all samples, specifically river and irrigation water samples on one farm, and no Listeria monocytogenes was detected throughout the study. Of the 80 characterised E. coli isolates, one harboured the stx2 virulence gene, while 43.75% (n=35) were multidrug resistant. This included 26.30% multidrug resistant E. coli isolates from production scenario one, where river water was used for irrigation, and 17.50% from the second production scenario that used borehole water for irrigation. Overall, a greater percentage of resistance phenotypes were from water E. coli isolates (52.50%), than isolates from spinach (37.50%). Escherichia coli isolates from spinach and irrigation water clustered together at high similarity values (>90%) using ERIC-PCR analysis. Conclusions: The results from this study provide valuable background information regarding the presence of multidrug resistant environmental E. coli throughout spinach production from farm, during processing and up to retail. Furthermore, the similarity of MDR E. coli isolates demonstrated transfer from irrigation water to spinach in both scenarios, reiterating that irrigation water for vegetables consumed raw, should comply with standardised microbiological safety guidelines. Significance and Impact of Study: Multidrug resistant E. coli presence throughout spinach production emphasises the necessity of increased surveillance of antimicrobial resistance in fresh produce and the production environment within a One Health paradigm to develop antimicrobial resistance mitigation strategies

Occurrence, Phenotypic and Molecular Characterization of Extended-Spectrum- and AmpC- β-Lactamase Producing Enterobacteriaceae Isolated From Selected Commercial Spinach Supply Chains in South Africa

The increasing occurrence of multidrug-resistant (MDR) extended-spectrum β-lactamase- (ESBL) and/or AmpC β-lactamase-producing Enterobacteriaceae in health care systems, the environment and fresh produce is a serious concern globally. Production practices, processing and subsequent consumption of contaminated raw fruit and vegetables represent a possible human transmission route. The purpose of this study was to determine the presence of ESBL/AmpC-producing Enterobacteriaceae in complete spinach supply chains and to characterize the isolated strains phenotypically (antimicrobial resistance profiles) and genotypically (ESBL/AmpC genetic determinants, detection of class 1, 2, and 3 integrons). Water, soil, fresh produce, and contact surface samples (n = 288) from two commercial spinach production systems were screened for ESBL/AmpC-producing Enterobacteriaceae. In total, 14.58% (42/288) of the samples were found to be contaminated after selective enrichment, plating onto chromogenic media and matrix-assisted laser desorption ionization time-of-flight mass spectrometry identity confirmation of presumptive ESBL/AmpC isolates. This included 15.28% (11/72) water and 12.12% (16/132) harvested- and processed spinach, while 25% (15/60) retail spinach samples were found to be contaminated with an increase in isolate abundance and diversity in both scenarios. Dominant species identified included Serratia fonticola (45.86%), Escherichia coli (20.83%), and Klebsiella pneumoniae (18.75%). In total, 48 (81.36%) isolates were phenotypically confirmed as ESBL/AmpC-producing Enterobacteriaceae of which 98% showed a MDR phenotype. Genotypic characterization (PCR of ESBL/AmpC resistance genes and integrons) further revealed the domination of the CTX-M Group 1 ESBL type, followed by TEM and SHV; whilst the CIT-type was the only plasmid-mediated AmpC genetic determinant detected. Integrons were detected in 79.17% (n = 38) of the confirmed ESBL/AmpC-producing isolates, of which we highlight the high prevalence of class 3 integrons, detected in 72.92% (n = 35) of the isolates, mostly in S. fonticola. Class 2 integrons were not detected in this study. This is the first report on the prevalence of ESBL/AmpC-producing Enterobacteriaceae isolated throughout commercial spinach production systems harboring class 1 and/or class 3 integrons in Gauteng Province, South Africa. The results add to the global knowledge base regarding the prevalence and characteristics of ESBL/AmpC-producing Enterobacteriaceae in fresh vegetables and the agricultural environment required for future risk analysis