Data Journalism at the Las Vegas Sun

Professional project report submitted in partial fulfillment of the requirements for the degree of Masters of Arts in Journalism from the School of Journalism, University of Missouri--Columbia.What was long known as computer-assisted reporting or "CAR" has finally become a mainstream practice at news organizations across the U.S. and around the globe. Advents in technology, open-source tools and the increasing importance of online presentation have pushed data journalism into new places. Case studies of stories and journalists at Honolulu Civil Beat, Thompson Reuters, La Nacion, Hoy and Oregon Public Broadcasting highlight the way CAR techniques are being used today.Includes bibliographic references

Disgust exposure and explicit emotional appraisal enhance the LPP in response to disgusted facial expressions

The influence of prior exposure to disgusting imagery and the conscious appraisal of facial expressions were examined in an event-related potential (ERP) experiment. Participants were exposed to either a disgust or a control manipulation and then presented with emotional and neutral expressions. An assessment of the gender of the face was required during half the blocks and an affective assessment of the emotion in the other half. The emotion-related early posterior negativity (EPN) and late positive potential (LPP) ERP components were examined for disgust and neutral stimuli. Results indicated that the EPN was enhanced for disgusted over neutral expressions. Prior disgust exposure modulated the middle phase of the LPP in response to disgusted but not neutral expressions, but only when the emotion of the face was explicitly evaluated. The late LPP was enhanced independently of stimuli when an emotional decision was made. Results demonstrated that exposure to disgusting imagery can affect the subsequent processing of disgusted facial expressions when the emotion is under conscious appraisal

Islands of history: the Late Neolithic timescape of Orkney

Orkney is internationally recognised for its exceptionally well-preserved Neolithic archaeology. The chronology of the Orcadian Neolithic is, however, relatively poorly defined. The authors analysed a large body of radiocarbon and luminescence dates, formally modelled in a Bayesian framework, to address the timescape of Orkney's Late Neolithic. The resultant chronology for the period suggests differences in the trajectory of social change between the ‘core’ (defined broadly as the World Heritage site) and the ‘periphery’ beyond. Activity in the core appears to have declined markedly fromc.2800 cal BC, which, the authors suggest, resulted from unsustainable local political tensions and social concerns

Will the darkness kill the laughs?

Sormenezko Audio Pieza (EP Musikala

The Chlamydia trachomatis Type III Secretion Chaperone Slc1 Engages Multiple Early Effectors, Including TepP, a Tyrosine-phosphorylated Protein Required for the Recruitment of CrkI-II to Nascent Inclusions and Innate Immune Signaling

Chlamydia trachomatis, the causative agent of trachoma and sexually transmitted infections, employs a type III secretion (T3S) system to deliver effector proteins into host epithelial cells to establish a replicative vacuole. Aside from the phosphoprotein TARP, a Chlamydia effector that promotes actin re-arrangements, very few factors mediating bacterial entry and early inclusion establishment have been characterized. Like many T3S effectors, TARP requires a chaperone (Slc1) for efficient translocation into host cells. In this study, we defined proteins that associate with Slc1 in invasive C. trachomatis elementary bodies (EB) by immunoprecipitation coupled with mass spectrometry. We identified Ct875, a new Slc1 client protein and T3S effector, which we renamed TepP (Translocated early phosphoprotein). We provide evidence that T3S effectors form large molecular weight complexes with Scl1 in vitro and that Slc1 enhances their T3S-dependent secretion in a heterologous Yersinia T3S system. We demonstrate that TepP is translocated early during bacterial entry into epithelial cells and is phosphorylated at tyrosine residues by host kinases. However, TepP phosphorylation occurs later than TARP, which together with the finding that Slc1 preferentially engages TARP in EBs leads us to postulate that these effectors are translocated into the host cell at different stages during C.trachomatis invasion. TepP co-immunoprecipitated with the scaffolding proteins CrkI-II during infection and Crk was recruited to EBs at entry sites where it remained associated with nascent inclusions. Importantly, C. trachomatis mutants lacking TepP failed to recruit CrkI-II to inclusions, providing genetic confirmation of a direct role for this effector in the recruitment of a host factor. Finally, endocervical epithelial cells infected with a tepP mutant showed altered expression of a subset of genes associated with innate immune responses. We propose a model wherein TepP acts downstream of TARP to recruit scaffolding proteins at entry sites to initiate and amplify signaling cascades important for the regulation of innate immune responses to Chlamydia.Fil: Chen, Yi-Shan. University of Duke; Estados UnidosFil: Bastidas, Robert J.. University of Duke; Estados UnidosFil: Saka, Hector Alex. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. University of Duke; Estados UnidosFil: Carpenter, Victoria K.. Duke University Medical Center; . University of Duke; Estados UnidosFil: Richards, Kristian L.. Miami University; Estados UnidosFil: Plano, Gregory V.. Miami University; Estados UnidosFil: Valdivia, Raphael H.. University of Duke; Estados Unido

Hidden Interactions in the Economics of Peace and Conflict

Peer reviewedPostprin