Effectiveness of Shield Termination Techniques Tested with TEM Cell and Bulk Current Injection

This paper presents experimental results of the effectiveness of various shield termination techniques. Each termination technique is evaluated by two independent noise injection methods; transverse electromagnetic (TEM) cell operated from 3 MHz 400 MHz, and bulk current injection (BCI) operated from 50 kHz 400 MHz. Both single carrier and broadband injection tests were investigated. Recommendations as to how to achieve the best shield transfer impedance (i.e. reduced coupled noise) are made based on the empirical data. Finally, the noise injection techniques themselves are indirectly evaluated by comparing the results obtained from the TEM Cell to those from BCI

Bulk Current Injection Testing of Cable Noise Reduction Techniques, 50 kHz to 400 MHz

This paper presents empirical results of cable noise reduction techniques as demonstrated using bulk current injection (BCI) techniques with radiated fields from 50 kHz - 400 MHz. It is a follow up to the two-part paper series presented at the Asia Pacific EMC Conference that focused on TEM cell signal injection. This paper discusses the effects of cable types, shield connections, and chassis connections on cable noise. For each topic, well established theories are compared with data from a real-world physical system

Exascale Agent-Based Modelling for Policy Evaluation in Real-Time (ExAMPLER)

Exascale computing can potentially revolutionise the way in which we design and build agent-based models (ABM) through, for example, enabling scaling up, as well as robust calibration and validation. At present, there is no exascale computing operating with ABM (that we are aware of), but pockets of work using High Performance Computing (HPC). While exascale computing is expected to become more widely available towards the latter half of this decade, the ABM community is largely unaware of the requirements for exascale computing for agent-based modelling to support policy evaluation. This project will engage with the ABM community to understand what computing resources are currently used, what we need (both in terms of hardware and software) and to set out a roadmap by which to make it happen

Overcoming the roadblocks to cardiac cell therapy using tissue engineering

Transplantations of various stem cells or their progeny have repeatedly improved cardiac performance in animal models of myocardial injury; however, the benefits observed in clinical trials have been generally less consistent. Some of the recognized challenges are poor engraftment of implanted cells and, in the case of human cardiomyocytes, functional immaturity and lack of electrical integration, leading to limited contribution to the heart’s contractile activity and increased arrhythmogenic risks. Advances in tissue and genetic engineering techniques are expected to improve the survival and integration of transplanted cells, and to support structural, functional, and bioenergetic recovery of the recipient hearts. Specifically, application of a prefabricated cardiac tissue patch to prevent dilation and to improve pumping efficiency of the infarcted heart offers a promising strategy for making stem cell therapy a clinical reality. [Display omitted

Reconciling the Greenland ice-core and radiocarbon timescales through the Laschamp geomagnetic excursion

Cosmogenic radionuclides, such as 10Be and 14C, share a common production signal, with their formation in the Earth's upper atmosphere modulated by changes to the geomagnetic field, as well as variations in the intensity of the solar wind. Here, we use this common production signal to compare between the radiocarbon (IntCal) and Greenland ice-core (GICC05) timescales, utilising the most pronounced cosmogenic production peak of the last 100,000 years – that associated with the Laschamp geomagnetic excursion circa 41,000 years ago. We present 54 new 14C measurements from a peat core (‘TP-2005’) from Tenaghi Philippon, NE Greece, contiguously spanning between circa 47,300 and 39,600 cal. BP, demonstrating a distinctive tripartite structure in the build up to the principal Laschamp production maximum that is not present in the consensus IntCal13 calibration curve. This is the first time that a continuous, non-reservoir corrected 14C dataset has been generated over such a long time span for this, the oldest portion of the radiocarbon timescale. This period is critical for both palaeoenvironmental and archaeological applications, with the replacement of Neanderthals by anatomically modern humans in Europe around this time. By placing our Tenaghi Philippon 14C dataset on to the Hulu Cave U-series timescale of Cheng et al. (2018) via Bayesian statistical modelling, the comparison of TP-2005 14C with Greenland 10Be fluxes also implicitly relates the underlying U-series and GICC05 timescales themselves. This comparison suggests that whilst these two timescales are broadly coherent, the IntCal13 timescale contains erroneous structure circa 40,000 cal. BP

Exascale Agent-Based Modelling for Policy Evaluation in Real-Time (ExAMPLER)

Exascale computing can potentially revolutionise the way in which we design and build agent-based models (ABM) through, for example, enabling scaling up, as well as robust calibration and validation. At present, there is no exascale computing operating with ABM (that we are aware of), but pockets of work using High Performance Computing (HPC). While exascale computing is expected to become more widely available towards the latter half of this decade, the ABM community is largely unaware of the requirements for exascale computing for agent-based modelling to support policy evaluation. This project will engage with the ABM community to understand what computing resources are currently used, what we need (both in terms of hardware and software) and to set out a roadmap by which to make it happen

MicroRNA-940 suppresses prostate cancer migration and invasion by regulating MIEN1.

BACKGROUND: MicroRNAs (miRNAs) are crucial molecules that regulate gene expression and hence pathways that are key to prostate cancer progression. These non-coding RNAs are highly deregulated in prostate cancer thus facilitating progression of the disease. Among the many genes that have gained importance in this disease, Migration and invasion enhancer 1 (MIEN1), a novel gene located next to HER2/neu in the 17q12 amplicon of the human chromosome, has been shown to enhance prostate cancer cell migration and invasion, two key processes in cancer progression. MIEN1 is differentially expressed between normal and cancer cells and tissues. Understanding the regulation of MIEN1 by microRNA may enable development of better targeting strategies. METHODS: The miRNAs that could target MIEN1 were predicted by in silico algorithms and microarray analysis. The validation for miRNA expression was performed by qPCR and northern blotting in cells and by in situ hybridization in tissues. MIEN1 and levels of other molecules upon miRNA regulation was determined by Western blotting, qPCR, and immunofluorescence. The functional effects of miRNA on cells were determined by wound healing cell migration, Boyden chamber cell invasion, clonal and colony formation assays. For knockdown or overexpression of the miRNA or overexpression of MIEN1 3'UTR, cells were transfected with the oligomiRs and plasmids, respectively. RESULTS: A novel miRNA, hsa-miR-940 (miR-940), identified and validated to be highly expressed in immortalized normal cells compared to cancer cells, is a regulator of MIEN1. Analysis of human prostate tumors and their matched normal tissues confirmed that miR-940 is highly expressed in the normal tissues compared to its low to negligible expression in the tumors. While MIEN1 is a direct target of miR-940, miR-940 alters MIEN1 RNA, in a quantity as well as cell dependent context, along with altering its downstream effectors. The miR-940 inhibited migratory and invasive potential of cells, attenuated their anchorage-independent growth ability, and increased E-cadherin expression, implicating its role in mesenchymal-to-epithelial transition (MET). CONCLUSIONS: These results, for the first time, implicate miR-940, a regulator of MIEN1, as a promising novel diagnostic and prognostic tool for prostate cancer

The Genome and Methylome of a Beetle with Complex Social Behavior,Nicrophorus vespilloides(Coleoptera: Silphidae)

Testing for conserved and novelmechanisms underlying phenotypic evolution requires a diversity of genomes available for comparisonspanning multiple independent lineages. For example, complex social behavior in insects has been investigated primarily witheusocial lineages, nearly all of which are Hymenoptera. If conserved genomic influences on sociality do exist, we need data from awider range of taxa that also vary in their levels of sociality. Here,we present the assembled and annotated genome of the subsocialbeetle Nicrophorus vespilloides, a species long used to investigate evolutionary questions of complex social behavior. We used thisgenome to address two questions. First, do aspects of life history, such as using a carcass to breed, predict overlap in gene modelsmore strongly than phylogeny? We found that the overlap in gene models was similar between N. vespilloides and all other insectgroups regardless of life history. Second, like other insects with highly developed social behavior but unlike other beetles, doesN. vespilloides have DNA methylation?We found strong evidence for an active DNA methylation system. The distribution of methylationwassimilar to other insects with exons having themostmethylatedCpGs. Methylation status appears highly conserved; 85%of themethylated genes in N. vespilloides are alsomethylated in the hymentopteran Nasonia vitripennis. The addition of this genomeadds a coleopteran resource to answer questions about the evolution and mechanistic basis of sociality and to address questionsabout the potential role of methylation in social behavior