Data catalog series for space science and applications flight missions. Volume 3B: Descriptions of data sets from low- and medium-altitude scientific spacecraft and investigations

The main purpose of the data catalog series is to provide descriptive references to data generated by space science flight missions. The data sets described include all of the actual holdings of the Space Science Data Center (NSSDC), all data sets for which direct contact information is available, and some data collections held and serviced by foreign investigators, NASA and other U.S. government agencies. This volume contains narrative descriptions of data sets from low and medium altitude scientific spacecraft and investigations. The following spacecraft series are included: Mariner, Pioneer, Pioneer Venus, Venera, Viking, Voyager, and Helios. Separate indexes to the planetary and interplanetary missions are also provided

Middle Wisconsinan Mammals, Stratigraphy, and Sedimentology at the Ketza River Site, Yukon Territory

Remains of Middle Wisconsinan mammoth, bison, horse, moose, hare, ground squirrel and lemming were recovered from sediments that stratigraphically underlie till of the Late Wisconsinan McConnell Glaciation along the Ketza River valley. The bone-bearing sediments are a complex of colluvium, fan, and outwash sediments. Similar fossil sites are likely to occur where glaciofluvial watercourses abut hillside deposits laid down prior to the onset of McConnell Glaciation.Les restes de mammouth, de bison, de cheval, d'orignal, de lièvre, d'écureuil et de lemming ont été recueillis dans des sédiments sous-jacents au till de la Glaciation de McConnell du Wisconsinien supérieur, le long de la vallée de Ketza. Les sédiments renfermant ces ossements représentent un complexe de colluvions, d'alluvions et de sédiments fluvio-glaciaires. D'autres sites fossilifères risquent de se trouver là où les cours d'eau fluvio-glaciaires traversent des colluvions déposées avant le début de la Glaciation de McConnell.Reste von Mammut, Bison, Pferd, amerikanischem Elch, Hase, Eichhörnchen und Lemming aus dem mittleren Wisconsinium sind in Sedimenten, die stratigraphisch unter dem Till der McConnell-Vereisung des späten Wisconsiniums liegen, entlang des Ketza River-Tals gefunden worden. Dièse knochenhaltigen Sedimente bestehen aus einem Komplex von Kolluvium, Alluvialfachern und glazifluviatilen Sedimenten. Ähnliche Fossil-Fundstellen befinden sich wahrscheinlich da, wo glazifluviatile Wasserläufe auf Berghang-Ablagerungen treffen, die vor dem Beginn der McConnell-Vereisung abgelagert worden sind

Cross-Disciplinary Analysis of the On-Farm Transition from Conventional to Organic Vegetable Production

This farm-scale analysis of the three-year transition to organic from conventional vegetable production tracked the changes in crop, soil, pest and management on two ranches (40 and 47 ha) in the Salinas Valley, California. Many small plantings of a diverse set of cash crop and cover crop species were used, as compared to only a few species in large monocultures in conventional production. The general trends with time were: increase in soil biological indicators, low soil nitrate pools, adequate crop nutrients, minor disease and weed problems, and sporadic mild insect damage. Some crops and cultivars consistently produced higher yields than others, relative to the maximum yield for a given crop. Differences in insect and disease damage were also observed. These results support the value of initially using a biodiverse set of taxa to reduce risk, then later choosing the best-suited varieties for optimal production. The grower used some principles of organic farming (e.g., crop diversity, crop rotation, and organic matter management), but also relied on substitution-based management, such as fertigation with soluble nutrients, initially heavy applications of organic pesticides, and use of inputs derived from off-farm sources. The organic transition was conducive to both production goals and environmental quality

Friction and wear of plasma-deposited diamond films

Reciprocating sliding friction experiments in humid air and in dry nitrogen and unidirectional sliding friction experiments in ultrahigh vacuum were conducted with a natural diamond pin in contact with microwave-plasma-deposited diamond films. Diamond films with a surface roughness (R rms) ranging from 15 to 160 nm were produced by microwave-plasma-assisted chemical vapor deposition. In humid air and in dry nitrogen, abrasion occurred when the diamond pin made grooves in the surfaces of diamond films, and thus the initial coefficients of friction increased with increasing initial surface roughness. The equilibrium coefficients of friction were independent of the initial surface roughness of the diamond films. In vacuum the friction for diamond films contacting a diamond pin arose primarily from adhesion between the sliding surfaces. In these cases, the initial and equilibrium coefficients of friction were independent of the initial surface roughness of the diamond films. The equilibrium coefficients of friction were 0.02 to 0.04 in humid air and in dry nitrogen, but 1.5 to 1.8 in vacuum. The wear factor of the diamond films depended on the initial surface roughness, regardless of environment; it increased with increasing initial surface roughness. The wear factors were considerably higher in vacuum than in humid air and in dry nitrogen

Microbial identification by mass cataloging

BACKGROUND: The public availability of over 180,000 bacterial 16S ribosomal RNA (rRNA) sequences has facilitated microbial identification and classification using hybridization and other molecular approaches. In their usual format, such assays are based on the presence of unique subsequences in the target RNA and require a prior knowledge of what organisms are likely to be in a sample. They are thus limited in generality when analyzing an unknown sample. Herein, we demonstrate the utility of catalogs of masses to characterize the bacterial 16S rRNA(s) in any sample. Sample nucleic acids are digested with a nuclease of known specificity and the products characterized using mass spectrometry. The resulting catalogs of masses can subsequently be compared to the masses known to occur in previously-sequenced 16S rRNAs allowing organism identification. Alternatively, if the organism is not in the existing database, it will still be possible to determine its genetic affinity relative to the known organisms. RESULTS: Ribonuclease T(1 )and ribonuclease A digestion patterns were calculated for 1,921 complete 16S rRNAs. Oligoribonucleotides generated by RNase T(1 )of length 9 and longer produce sufficient diversity of masses to be informative. In addition, individual fragments or combinations thereof can be used to recognize the presence of specific organisms in a complex sample. In this regard, 140 strains out of 1,921 organisms (7.3%) could be identified by the presence of a unique RNase T(1)-generated oligoribonucleotide mass. Combinations of just two and three oligoribonucleotide masses allowed 54% and 72% of the specific strains to be identified, respectively. An initial algorithm for recovering likely organisms present in complex samples is also described. CONCLUSION: The use of catalogs of compositions (masses) of characteristic oligoribonucleotides for microbial identification appears extremely promising. RNase T(1 )is more useful than ribonuclease A in generating characteristic masses, though RNase A produces oligomers which are more readily distinguished due to the large mass difference between A and G. Identification of multiple species in mixtures is also feasible. Practical applicability of the method depends on high performance mass spectrometric determination, and/or use of methods that increase the one dalton (Da) mass difference between uracil and cytosine

Perceptions of Grief Education in Accredited Counseling Programs: Recommendations for Counselor Education

As grief can be considered a critical life event, unresolved grief can interfere with quality of life, affecting lifestyle, behavior, emotional strength, and cognitive function. Unresolved grief can even result in suicidal ideation. Counselors can and often do work with grief issues in clients and can promote positive outcomes for grieving clients by addressing personal loss and helping clients process grief related issues. This study was based on an analysis of students within counseling programs accredited by the Council for Accreditation of Counseling and Related Educational Programs based on earlier research conducted by one of the authors. Self-perception of competency in counseling clients who are having grief-related issues from loss was assessed using the Grief Counseling Competency Scale. By analyzing results of the Grief Counseling Competency Scale, participants showed a need for further training in grief counseling skills and theory. Results and implications of the quantitative analysis as well as suggestions for further study are discussed

Bacterial genotyping by 16S rRNA mass cataloging

BACKGROUND: It has recently been demonstrated that organism identifications can be recovered from mass spectra using various methods including base-specific fragmentation of nucleic acids. Because mass spectrometry is extremely rapid and widely available such techniques offer significant advantages in some applications. A key element in favor of mass spectrometric analysis of RNA fragmentation patterns is that a reference database for analysis of the results can be generated from sequence information. In contrast to hybridization approaches, the genetic affinity of any unknown isolate can in principle be determined within the context of all previously sequenced 16S rRNAs without prior knowledge of what the organism is. In contrast to the original RNase T(1 )cataloging method, when digestion products are analyzed by mass spectrometry, products with the same base composition cannot be distinguished. Hence, it is possible that organisms that are not closely related (having different underlying sequences) might be falsely identified by mass spectral coincidence. We present a convenient spectral coincidence function for expressing the degree of similarity (or distance) between any two mass-spectra. Trees constructed using this function are consistent with those produced by direct comparison of primary sequences, demonstrating that the inherent degeneracy in mass spectrometric analysis of RNA fragments does not preclude correct organism identification. RESULTS: Neighbor-joining trees for important bacterial pathogens were generated using distances based on mass spectrometric observables and the spectral coincidence function. These trees demonstrate that most pathogens will be readily distinguished using mass spectrometric analyses of RNA digestion products. A more detailed, genus-level analysis of pathogens and near relatives was also performed, and it was found that assignments of genetic affinity were consistent with those obtained by direct sequence comparisons. Finally, typical values of the coincidence between organisms were also examined with regard to phylogenetic level and sequence variability. CONCLUSION: Cluster analysis based on comparison of mass spectrometric observables using the spectral coincidence function is an extremely useful tool for determining the genetic affinity of an unknown bacterium. Additionally, fragmentation patterns can determine within hours if an unknown isolate is potentially a known pathogen among thousands of possible organisms, and if so, which one

NCER Assistance Agreement Annual Progress Report for Grant #83582401 - Assessment of Stormwater Harvesting via Manage Aquifer Recharge (MAR) to Develop New Water Supplies in the Arid West: The Salt Lake Valley Example

The aims of the original proposed project remain the same, that is, to test the hypothesis that Managed Aquifer Recharge (MAR) for stormwater harvesting is a technically feasible, socially and environmentally acceptable, economically viable, and permittable option for developing new water supplies for arid Western urban ecosystems experiencing increasing population, and climate change pressures on existing water resources. The project is being carried out via three distinct but integrated components that include: 1) Monitoring of existing distributed Managed Aquifer Recharge (MAR) harvesting schemes involving a growing number of demonstration Green Infrastructure (GI) test sites; 2) Integrated stormwater/vadose zone/groundwater/ ecosystem services modeling; and 3) Social Science research assessing Stakeholder attitudes, and solicitation of their collaboration on feasible distributed MAR scenario development and subsequent analysis of scenario outcomes. Each of these components are discussed separately in the material presented below

The Validity of Peer Review in a General Medicine Journal

All the opinions in this article are those of the authors and should not be construed to reflect, in any way, those of the Department of Veterans Affairs