Extraction of Cerium (IV) Using Di–n-butylsulfoxide in Chloroform from Nitric Acid and Determination with Arsenazo (III) as Chromogenic Reagent

A new and advantageous extraction procedure was developed for extraction of cerium (IV) from nitric acid with di-n-butylsulfoxide (DBSO) in perchloric acid. The extracted Ce (IV) was determined spectrophotometrically using 0.01 % solution of arsenazo-III in 3Mper-chloric acid. Various parameters such as equilibration time, metal ion concentration, effect of temperature and diverse ions on the extraction of the Ce (IV) were established. U (VI), Th (IV) and Pb (II) interfere whereas only phosphate and fluoride suppress the extraction among anions. The stoichiometric composition of the cerium complex withDBSO was determined by slope analysis and found to be 1:4. The process of extraction was found to be exothermic. Deionized water was the most appropriate solvent for back extraction of cerium. This method is easier and more sensitive than many of the reported procedures.KEYWORDS Cerium, extraction, DBSO, perchloric acid, arsenazo-II

Development and characterization of a large set of microsatellite markers for grape phylloxera (Daktulosphaira vitifoliae Fitch)

This study describes novel simple sequence repeat (SSR) primers from a genomic DNA sequence of the grape phylloxera. A total of 130 SSR primers were designed from 145 unique sequences with di, tri, tetra and penta simple sequence repeats. The SSR primers were tested on DNA from 10 grape phylloxera strains chosen for their behavioral and geographic diversity. Eightynine primers generated easy to score alleles with standardized  conditions of amplification. Twenty-eight new and four previously published markers were selected to genotype 32 root and leaf phylloxera samples in order to identify reliable markers for future genetic diversity and phylloxera population studies. SSR data from these samples was also used to determine the frequency of null alleles, and locus specific estimates of population differentiation and clustering. Up to six alleles were detected with a mean expected heterozygosity (He) of 0.51. The observed heterozygosity (Ho) was 0.73 and the majority of markers had higher Ho values. Null allees for four markers were considered to be the result of homozygous genotypes. The 89 SSR loci developed in this study represent a new and informative set of markers that are easy to combine for multi-loading and suitable for large-scale genetic analyses of population structure, genetic diversity, and the origin of host specific strains in grape phylloxera.

Microsatellite Identification in Solanaceae Crops Associated with Nucleoside Diphosphate Kinase (NDK) Specific to Abiotic Stress Tolerance through in silico Analysis

Abiotic stress often causes a series of morphological, physiological, biochemical and molecular changes that affect plant growth, development and productivity. To cope with abiotic stresses, it is necessary to understand plant responses to stresses that disturb homeostatic equilibrium at the cellular and molecular level. Genomic information on Capsicum annuum has been explored to identify microsatellite markers associated with abiotic stress tolerance and assign them to cognate functional groups related to specific stress responses. Several in silico methods have been used to identify simple sequence repeats associated with stress responsive gene candidates in Capsicum annuum. In this study, a microsatellite marker has been identified in Capsicum annuum associated with Nucleoside Diphosphate Kinase (NDK) having multiple environmental stress tolerance (oxidative, high temperature and salt stress) and which is also highly conserved in crops of Solanaceae. These are house-keeping enzymes that maintain intracellular levels of all nucleoside triphosphates (NTP) with the exception of adenosine triphosphate (ATP). These are also involved in phytochrome A response, UV-B signaling, auxin responses and oxidative stress signaling

Ooid distribution and fabric in the Miaolingian of Xiaweidian Section, Beijing (North China Platform)

This study is an attempt to describe the distribution and fabric of the Cambrian ooids from the Xuzhuang, Zhangxia and Gushan formations at the Xiaweidian section. The oolitic banks occupy the upper parts of the 3rd order depositional sequences recognized for these formations. Petrographical techniques were applied to describe the sedimentary features of ooid grains. Different characteristics of ooids including size distribution, composition, morphology and the internal and external cortical architecture were taken into consideration. Radial-concentric, micritic, superficial, composite, pseudo-oids, neomorphosed and geopetal ooids are properly studied under the microscope. Different fabrics of ooids have been linked to their different depositional settings, and a variety of sub-environments has been established. The oolitic grain banks are composed mainly of calcite, with noteworthy presence of aragonite and dolomite. The two-fold role of microorganisms during and after the formation of ooids can be recognized under the microscope. The mechanism of ooids fabric modification has been elaborated in detail. Firstly, the dark laminae in several ooids most probably show the remains of filamentous cyanobacteria taking part in the construction of ooids. Secondly, they destroy the cortex through boring, which is then subsequently filled by aragonite. In order to apprehend the sedimentological features of the Miaolingian strata in the Xiaweidian section, this research highlights the distribution of oolites and their resultant fabric in response to relative sea-level variations. The Miaolingian ooids in the Xiaweidian section provide a good reference example of the depositional pattern of oolitic grain banks

A nonsense mutation in S-antigen (p.Glu306*) causes Oguchi disease

Contains fulltext : 110974.pdf (publisher's version ) (Open Access)PURPOSE: Genetic studies were performed to identify the causative mutation in a 15-year-old girl diagnosed with congenital stationary night blindness (CSNB) presenting Mizuo-Nakamura phenomenon, a typical Oguchi disease symptom. The patient also had dural sinus thrombosis (DST), thrombocytopenia, and systemic lupus erythematosus (SLE). METHODS: Mutation analysis was done by sequencing two candidate genes, S-antigen (SAG; arrestin 1), associated with Oguchi type 1, and rhodopsin kinase (GRK1), associated with Oguchi type 2. In addition, the C677T variation in the methylenetetrahydrofolate reductase (MTHFR) gene was also screened in the family, to determine its probable association with hyperhomocysteinemia in the patient. RESULTS: Sequencing of the SAG and GRK1 resulted in identifying a novel homozygous nonsense mutation (c.916G>T; p.Glu306*) in SAG, which in unaffected siblings either was present in a heterozygous state or absent. The C677T heterozygous allele in the MTHFR gene was found to be associated with hyperhomocysteinemia in the patient and other family members. CONCLUSIONS: This is the first report of Oguchi type 1 in a Pakistani patient due to a nonsense mutation (c.916G>T; p.Glu306*) in SAG. The neurologic and hematological abnormalities likely are not associated with the SAG variant

Orbital and physical parameters of eclipsing binaries from the ASAS catalogue - IV. A 0.61 + 0.45 M_sun binary in a multiple system

We present the orbital and physical parameters of a newly discovered low-mass detached eclipsing binary from the All-Sky Automated Survey (ASAS) database: ASAS J011328-3821.1 A - a member of a visual binary system with the secondary component separated by about 1.4 seconds of arc. The radial velocities were calculated from the high-resolution spectra obtained with the 1.9-m Radcliffe/GIRAFFE, 3.9-m AAT/UCLES and 3.0-m Shane/HamSpec telescopes/spectrographs on the basis of the TODCOR technique and positions of H_alpha emission lines. For the analysis we used V and I band photometry obtained with the 1.0-m Elizabeth and robotic 0.41-m PROMPT telescopes, supplemented with the publicly available ASAS light curve of the system. We found that ASAS J011328-3821.1 A is composed of two late-type dwarfs having masses of M_1 = 0.612 +/- 0.030 M_sun, M_2 = 0.445 +/- 0.019 M_sun and radii of R_1 = 0.596 +/- 0.020 R_sun, R_2 = 0.445 +/- 0.024 R_sun, both show a substantial level of activity, which manifests in strong H_alpha and H_beta emission and the presence of cool spots. The influence of the third light on the eclipsing pair properties was also evaluated and the photometric properties of the component B were derived. Comparison with several popular stellar evolution models shows that the system is on its main sequence evolution stage and probably is more metal rich than the Sun. We also found several clues which suggest that the component B itself is a binary composed of two nearly identical ~0.5 M_sun stars.Comment: 12 pages, 7 figures, 7 tables, to appear in MNRA

Unusual cause of a painful right testicle in a 16-year-old man: a case report

<p>Abstract</p> <p>Introduction</p> <p>Urgent surgical exploration of the scrotum of a child or teenager who presents with a painful and swollen testicle is paramount if testicular torsion is not to be missed. It is extremely rare for a non-scrotal pathology to present with acute scrotal signs. Here we present such a rare case and emphasize the importance of being aware of this potential clinical pitfall.</p> <p>Case presentation</p> <p>A 16-year-old Caucasian man presented as a surgical emergency with a five to six hour history of a painful, red, and swollen right hemiscrotum. He also complained of vague lower abdominal pain, vomiting, and watery diarrhea. He had a temperature of 38.5°C and a tender, red, and swollen right hemiscrotum. The right testicle appeared elevated. He was mildly tender in his central and upper abdomen and less so in the lower abdomen. No convincing localizing abdominal signs were noted. He had an increased white cell count (15 × 10⁹/L) and C-reactive protein (CRP; 300 mg/L). Urgent right hemiscrotal exploration revealed about 5 ml of pus in the tunica vaginalis and a normal testicle. A right iliac fossa incision identified the cause: a perforated retrocecal appendix. Appendectomy was performed, and both the abdomen and scrotum washed copiously with saline before closure. The patient made an uneventful recovery.</p> <p>Conclusion</p> <p>Acute appendicitis presenting with scrotal signs due to a patent processus vaginalis is an extremely rare clinical entity. To date, fewer than five such cases have been reported in the medical literature. It is, therefore, extremely important to be aware of this unusual clinical scenario, as only a high index of suspicion will enable prompt, successful management of both the appendicitis and the scrotal abscess.</p

A catalogue of bright (K <9) M dwarfs

Using the Position and Proper Motion Extended-L (PPMXL) catalogue, we have used optical and near-infrared colour cuts together with a reduced proper motion cut to find bright M dwarfs for future exoplanet transit studies. PPMXL's low proper motion uncertainties allow us to probe down to smaller proper motions than previous similar studies. We have combined unique objects found with this method to that of previous work to produce 8479 K <9 M dwarfs. Low-resolution spectroscopy was obtained of a sample of the objects found using this selection method to gain statistics on their spectral type and physical properties. Results show a spectral-type range of K7-M4V. This catalogue is the most complete collection of K <9 M dwarfs currently available and is made available here.Peer reviewe