Stratigraphy and allogenic controls of the western Portugal Cretaceous: an updated synthesis

The stratigraphy of the western Portugal on-shore Cretaceous record (western Iberian margin, Lusitanian Basin) is described, including formal units and a selection of informal units prevailing in the geological literature. This paper is a synthesis based on a review of previous works, but with an innovative emphasis on the interpretation of eustatic and tectonic controls. The sedimentary record is dominated by siliciclastics and comprises fluvial and deltaic coastal marine siliciclastic systems, as well as extensive deposits of shallow marine carbonate platforms, both open and rimmed. Several regional unconformities and transgressive/regressive cycles are identified and the allogenic controls interpreted, namely the geodynamic events along the boundaries of the Iberian plate. Above the Berriasian deposits belonging to the Upper Jurassic cycle, the five main unconformity-bounded units are: (1) upper Berriasian–lower Barremian, (2) upper Barremian–lower Aptian, (3) upper Aptian–uppermost Cenomanian, (4) mid lower Turonian–lower Campanian and (5) middle Campanian–Maastrichtian. These units show transgressive peaks in the lower Hauterivian, lower Aptian, base of the upper Cenomanian and mid lower Turonian. The general trend of the Lower Cretaceous reflects the transition from late rifting to passive margin, with the last break-up unconformity dated as late Aptian. The Lusitanian Basin achieved full infill by the Cenomanian, when a large carbonate platform extended far inland. The later deposits were preserved only in the northern sector and the accompanying unconformities reflect transpressive intraplate stresses generated in boundaries of the plate with Africa and Eurasia. With very low accommodation being created throughout the Late Cretaceous, fluvial deposits were dominant, including a few marine levels related with eustatic rises in the early Turonian, the Coniacian, the early Campanian and the Maastrichtian

Methylation status of ANAPC1, CDKN2A and TP53 promoter genes in individuals with gastric cancer

Gastric cancer is the forth most frequent malignancy and the second most common cause of cancer death worldwide. DNA methylation is the most studied epigenetic alteration, occurring through a methyl radical addition to the cytosine base adjacent to guanine. Many tumor genes are inactivated by DNA methylation in gastric cancer. We evaluated the DNA methylation status of ANAPC1, CDKN2A and TP53 by methylation-specific PCR in 20 diffuse- and 26 intestinal-type gastric cancer samples and 20 normal gastric mucosa in individuals from Northern Brazil. All gastric cancer samples were advanced stage adenocarcinomas. Gastric samples were surgically obtained at the João de Barros Barreto University Hospital, State of Pará, and were stored at -80°C before DNA extraction. Patients had never been submitted to chemotherapy or radiotherapy, nor did they have any other diagnosed cancer. None of the gastric cancer samples presented methylated DNA sequences for ANAPC1 and TP53. CDKN2A methylation was not detected in any normal gastric mucosa; however, the CDKN2A promoter was methylated in 30.4% of gastric cancer samples, with 35% methylation in diffuse-type and 26.9% in intestinal-type cancers. CDKN2A methylation was associated with the carcinogenesis process for ~30% diffuse-type and intestinal-type compared to non-neoplastic samples. Thus, ANAPC1 and TP53 methylation was probably not implicated in gastric carcinogenesis in our samples. CDKN2A can be implicated in the carcinogenesis process of only a subset of gastric neoplasias

Sizing of an autonomous microgrid considering droop control

Autonomous microgrids are a suitable solution for off-grid electrification in terms of costs and reliability. The correct sizing of its generation and storage systems ensures efficient utilization of the available energy resources. Generally, many sizing approaches assume optimized energy management strategies that rely on central control architectures. However, these architectures are not always available, especially in limited investment microgrid projects. For this reason, the study of operation scenarios based on decentralized control strategy such as droop control is relevant. Based on this, this paper aims to evaluate the impact of the droop control over the sizing results of a solar/wind/battery/diesel microgrid. For this purpose, a case study of a sizing problem is presented, including the formulation and modelling. Results are presented comparing an hourly optimized energy management scenario with multiple values of the droop control parameters. Simulations results indicate that a competitive total cost can be obtained if the droop parameters are calculated considering the microgrid sizing results. Based on this, a generalizable design methodology for this purpose is presented.Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.Intelligent Electrical Power Grid

Puberty arises with testicular alterations and defective AMH expression in rams prenatally exposed to testosterone

The male gonadal tissue can be a sensitive target to the reprogramming effects of testosterone (T) during prenatal development. We have demonstrated that male lambs born to dams receiving T during pregnancyda model system to the polycystic ovary syndrome (PCOS)d show a decreased number of germ cells early in life, and when adult, a reduced amount of sperm and ejaculate volume. These findings are a key to put attention to the male offspring of women bearing PCOS, as they are exposed to increased levels of androgen during pregnancy which can reprogram their reproductive outcome. A possible origin of these defects can be a disruption in the expression of the anti-Müllerian hormone (AMH), due to its critical role in gonadal function at many postnatal stages. Therefore, we addressed the impact of prenatal T excess on the expression of AMH and factors related to its expression like AP2, SOX9, FSHR, and AR in the testicular tissue through real-time PCR during the peripubertal age. We also analyzed the testicular morphology and quantified the number of Sertoli cells and germ cells to evaluate any further defect in the testicle. Experiments were performed in rams at 24 wk of age, hence, prior puberty. The experimental animals (T-males) consisted of rams born to mothers receiving 30 mg testosterone twice a wk from Day 30 to 90 of pregnancy and then increased to 40 mg until Day 120 of pregnancy. The control males (C-males) were born to mothers receiving the vehicle of the hormone. We found a significant increase in the expression of the mRNA of AMH and SOX9, but not of the AP2, FHSR nor AR, in the T-males. Moreover, T-males showed a dramatic decrease in the number of germ cells, together with a decrease in the weight of their testicles. The findings of the present study show that before puberty, T-males are manifesting clear signs of disruption in the gonadal functions probably due to an alteration in the expression pattern of the AMH gene. The precise way by which T reprograms the expression of AMH gene remains to be established.Fil: Recabarren, S. E.. Universidad de Concepción; ChileFil: Recabarren, Mónica. Universidad de Concepción; ChileFil: Sandoval, D.. Universidad de Concepción; ChileFil: Carrasco, A.. Universidad de Concepción; ChileFil: Padmanabhan, Vasantha. University of Michigan; Estados UnidosFil: Rey, Rodolfo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas ; Argentina. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Richter, Hans. Universidad Austral de Chile; ChileFil: Perez Marin, C. C.. Universidad de Córdoba; EspañaFil: Sir Petermann, Teresa. Universidad de Chile; ChileFil: Rojas Garcia, P.P.. Universidad de Concepción; Chil