Controls on luminescence signals in lake sediment cores:A study from Lake Suigetsu, Japan

The luminescence characteristics of sediments are driven by a range of environmental factors and can be used as indicators of both local and regional environmental shifts. Hence, rapid luminescence profiling techniques are increasingly employed during multiproxy analysis of sediment cores, overcoming the practical limitations of traditional (dating) methods. One emerging application of luminescence profiling is in the palaeoenvironmental investigation of lake cores. This study demonstrates the versatility of rapid core profiling using portable optically stimulated luminescence and laboratory profiling techniques for appraising the luminescence characteristics of the Lake Suigetsu (Japan) sediment cores. These techniques were employed across four key time periods, each selected for their unique environmental context and significance on either a local or global scale, in order to identify relationships between down-core luminescence and environmental change. We demonstrate that the luminescence characteristics of the cores are susceptible to a range of environmental perturbations and can therefore act as proxies of past change. Additionally, the quantification of these luminescence signals, alongside an assessment of dose rate variations down-core, supports the notion that future luminescence dating is feasible. The results of this analysis contribute to the wider understanding of the application of luminescence techniques – both profiling and dating – to lake sediment cores

Novel observations of East Asian Summer Monsoon evolution during Glacial Termination II from Lake Suigetsu, Japan

The dataset includes n-alkane and n-alkanoic acid concentrations, n-alkane index values and stable hydrogen isotope analysis values of n-alkanoic acids from the Lake Suigetsu sediment cores. The dataset spans from ~125 – 133 ka BP and samples were taken and analysed contiguously to each represent ~58-year (n=56) or ~112 years (n=4) of integrated time. These results were used to reconstruct catchment evolution and East Asian Summer Monsoon behaviour across Glacial Termination II

The contemporary stable isotope hydrology of Lake Suigetsu and surrounding catchment (Japan) and its implications for sediment-derived palaeoclimate records

The Lake Suigetsu sediment cores exemplify a high-quality archive of palaeoclimatic change in East Asia during the past 150 ka. Robust interpretation of stable isotope-based proxy reconstructions from the Suigetsu cores can be aided by a greater understanding of the factors affecting the isotope composition of the lake and how it relates to that of precipitation. Here we use extended contemporary monitoring to establish the factors affecting the stable isotope composition (δ18O, δ2H and d-excess) of precipitation, river water and lake water in the catchment surrounding Lake Suigetsu, central Japan. We show that the composition of precipitation is influenced by the dual East Asian Monsoon system, producing minima in δ18O and δ2H and semi-annually varying d-excess values across the year. These signals are then transferred to the lake system, where they are combined with secondary local influences on lake water composition: homogenisation with existing catchment waters, a catchment transit lag, the interaction with saline water from the nearby Sea of Japan, and evaporative enrichment during summer. Our observations suggest that the palaeo-isotope composition of Lake Suigetsu was closely related to the behaviour of the East Asian Monsoon. We highlight lake stratification and proxy seasonality as critical components of signal interpretation

East Asian Monsoon behaviour reflected in the stable isotope hydrology of Lake Suigetsu and surrounding catchment, Japan

Stable oxygen and hydrogen isotope composition of water samples from the Five Lakes of Mikata catchment, central Japan. Samples consist of rainwater, river water and lake water. Sampling information and accompanying weather data are also provided. (Sheet 1) Temperature and salinity variations with depth within Lake Suigetsu across six sampling dates. (Sheet 2

Safety and immunogenicity of the ChAdOx1 nCoV-19 vaccine against SARS-CoV-2: a preliminary report of a phase 1/2, single-blind, randomised controlled trial.

BACKGROUND: The pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) might be curtailed by vaccination. We assessed the safety, reactogenicity, and immunogenicity of a viral vectored coronavirus vaccine that expresses the spike protein of SARS-CoV-2. METHODS: We did a phase 1/2, single-blind, randomised controlled trial in five trial sites in the UK of a chimpanzee adenovirus-vectored vaccine (ChAdOx1 nCoV-19) expressing the SARS-CoV-2 spike protein compared with a meningococcal conjugate vaccine (MenACWY) as control. Healthy adults aged 18-55 years with no history of laboratory confirmed SARS-CoV-2 infection or of COVID-19-like symptoms were randomly assigned (1:1) to receive ChAdOx1 nCoV-19 at a dose of 5 × 1010 viral particles or MenACWY as a single intramuscular injection. A protocol amendment in two of the five sites allowed prophylactic paracetamol to be administered before vaccination. Ten participants assigned to a non-randomised, unblinded ChAdOx1 nCoV-19 prime-boost group received a two-dose schedule, with the booster vaccine administered 28 days after the first dose. Humoral responses at baseline and following vaccination were assessed using a standardised total IgG ELISA against trimeric SARS-CoV-2 spike protein, a muliplexed immunoassay, three live SARS-CoV-2 neutralisation assays (a 50% plaque reduction neutralisation assay [PRNT50]; a microneutralisation assay [MNA50, MNA80, and MNA90]; and Marburg VN), and a pseudovirus neutralisation assay. Cellular responses were assessed using an ex-vivo interferon-γ enzyme-linked immunospot assay. The co-primary outcomes are to assess efficacy, as measured by cases of symptomatic virologically confirmed COVID-19, and safety, as measured by the occurrence of serious adverse events. Analyses were done by group allocation in participants who received the vaccine. Safety was assessed over 28 days after vaccination. Here, we report the preliminary findings on safety, reactogenicity, and cellular and humoral immune responses. The study is ongoing, and was registered at ISRCTN, 15281137, and ClinicalTrials.gov, NCT04324606. FINDINGS: Between April 23 and May 21, 2020, 1077 participants were enrolled and assigned to receive either ChAdOx1 nCoV-19 (n=543) or MenACWY (n=534), ten of whom were enrolled in the non-randomised ChAdOx1 nCoV-19 prime-boost group. Local and systemic reactions were more common in the ChAdOx1 nCoV-19 group and many were reduced by use of prophylactic paracetamol, including pain, feeling feverish, chills, muscle ache, headache, and malaise (all p<0·05). There were no serious adverse events related to ChAdOx1 nCoV-19. In the ChAdOx1 nCoV-19 group, spike-specific T-cell responses peaked on day 14 (median 856 spot-forming cells per million peripheral blood mononuclear cells, IQR 493-1802; n=43). Anti-spike IgG responses rose by day 28 (median 157 ELISA units [EU], 96-317; n=127), and were boosted following a second dose (639 EU, 360-792; n=10). Neutralising antibody responses against SARS-CoV-2 were detected in 32 (91%) of 35 participants after a single dose when measured in MNA80 and in 35 (100%) participants when measured in PRNT50. After a booster dose, all participants had neutralising activity (nine of nine in MNA80 at day 42 and ten of ten in Marburg VN on day 56). Neutralising antibody responses correlated strongly with antibody levels measured by ELISA (R2=0·67 by Marburg VN; p<0·001). INTERPRETATION: ChAdOx1 nCoV-19 showed an acceptable safety profile, and homologous boosting increased antibody responses. These results, together with the induction of both humoral and cellular immune responses, support large-scale evaluation of this candidate vaccine in an ongoing phase 3 programme. FUNDING: UK Research and Innovation, Coalition for Epidemic Preparedness Innovations, National Institute for Health Research (NIHR), NIHR Oxford Biomedical Research Centre, Thames Valley and South Midland's NIHR Clinical Research Network, and the German Center for Infection Research (DZIF), Partner site Gießen-Marburg-Langen

Global COVID-19 lockdown highlights humans as both threats and custodians of the environment

The global lockdown to mitigate COVID-19 pandemic health risks has altered human interactions with nature. Here, we report immediate impacts of changes in human activities on wildlife and environmental threats during the early lockdown months of 2020, based on 877 qualitative reports and 332 quantitative assessments from 89 different studies. Hundreds of reports of unusual species observations from around the world suggest that animals quickly responded to the reductions in human presence. However, negative effects of lockdown on conservation also emerged, as confinement resulted in some park officials being unable to perform conservation, restoration and enforcement tasks, resulting in local increases in illegal activities such as hunting. Overall, there is a complex mixture of positive and negative effects of the pandemic lockdown on nature, all of which have the potential to lead to cascading responses which in turn impact wildlife and nature conservation. While the net effect of the lockdown will need to be assessed over years as data becomes available and persistent effects emerge, immediate responses were detected across the world. Thus initial qualitative and quantitative data arising from this serendipitous global quasi-experimental perturbation highlights the dual role that humans play in threatening and protecting species and ecosystems. Pathways to favorably tilt this delicate balance include reducing impacts and increasing conservation effectiveness