Caracterización de Heridas Penetrantes Causada por Proyectiles de Fuego Calibre (9 Mm Corto .380” Auto) y (.38” Spl) a Diferentes Distancias en Tórax de Cadáver de Perro, Arequipa 2018

OBJETIVO: Caracterizar las lesiones que son ocasionadas por proyectiles de fuego calibre (9 mm Corto y .38’’ SPL a distancias de 0, 15 y 30 cm en tórax de cadáver de perro. METODOLOGIA: se realizó un trabajo de investigación de tipo descriptivo guiándonos de protocolos de necropsia y fotografías. En el trabajo se usaron 6 cadáveres de perros de sexo masculino, mayores de 1 año, los cuales fueron donados por diferentes clínicas veterinarias de la Ciudad de Arequipa. Las armas que se usaron fueron un revolver calibre .38” SPL y una pistola calibre 9 mm, se describieron las lesiones que causan estas dos armas a diferentes distancias en tórax de cadáveres de perros. RESULTADOS: El revólver calibre .38” SPL, a una distancia de 0 cm causa una mayor lesión que la pistola calibre 9mm a nivel de piel, la marca de tatuaje en ambas armas es visible a una distancia de 0 cm, pero a una distancia de 15 cm solo es visible el tatuaje de la pistola calibre 9 mm. Los órganos más afectados fueron los pulmones, ocasionando perforación de estos a distintos niveles, afectando uno o más lóbulos de estos. CONCLUSIONES: El grado de penetración de los proyectiles en todos los casos fue de un 100%. El trayecto de pudo identificar por medio de la ubicación de los orificios de entrada y salida, así como la ubicación de la perforación en órganos. Se propuso un informe pericial forense para casos por impacto de proyectil de fuego en perros Palabras Clave: Proyectil de fuego, Tórax, 9 mm Corto, .38” SPL, Cadáver de perro.Tesi

The Impacts Of Neighbourhood Traffic Management

A major traffic-related problem faced by residents is speeding, which not only causes safety concerns, but also noise issues. Traffic calming is a much favoured traffic management tool employed by road controlling authorities to primarily reduce vehicle speed, hence improve community liveability. This research aimed to investigate the impacts of traffic calming on speed, safety and traffic noise. The objectives included developing models for the prediction of speed and noise on traffic-calmed streets, and providing guidance for good design practices. Speeds of individual vehicles as they approached and crossed traffic calming devices were observed in order to identify the behaviour of individual drivers. Results indicated that the speed hump and the raised angled slow point produced the largest speed reductions and least variation in speeds, while mid-block narrowings had no significant speed changes. Inter-device speed was found to be mainly controlled by the separation between devices. 85th percentile speeds at distances from calming devices were 40 – 45 km/h for vertical deflections and 45 – 55 km/h for horizontal deflections. Speeds on approach to speed humps were found to be influenced by the distance available on the approaches, while operating speed at the speed humps were partly influenced by the hump width relative to the road width. There was evidence of safety benefits of traffic calming overall, despite mid-block crashes increasing post-calming. However, there was no association between the traffic calming and the crashes, which appeared to probably be due to other factors, human factors in particular. Noise levels produced by light vehicles across speed humps were in fact lower than on a flat section of road, given their respective mean speeds. At a reference speed of 25 km/h, noise levels produced over the 100 mm hump were 3.6 dBA higher than those produced by the 75 mm hump

Phage mediated horizontal transfer of the sopE1 gene increases enteropathogenicity of Salmonella enterica serotype Typhimurium for calves

Epidemiological evidence shows that the sopE1 gene is associated with Salmonella Typhimurium phage types causing epidemics in cattle. In this study we demonstrate that horizontal transfer of the sopE1 gene by lysogenic conversion with the SopEΦ increased enteropathogenicity of S. Typhimurium in the bovine ligated ileal loop model. These data support the hypothesis that phage mediated horizontal transfer of the sopE1 gene contributes to the emergence of epidemic cattle-associated S. Typhimurium clone

Crowdfunding vs. Taxes: Does the payment vehicle influence WTP for Ecosystem Services protection?

The effect of the payment vehicle (PV) on economic valuation estimates has been addressed since the early literature on stated preferences studies. Particularly, some studies have focused on willingness to pay (WTP) sensitivity to mandatory/collective vs. voluntary/individual PVs, by comparing tax increases or redistribution based on specific taxes with donation-like contributions. These two payment schemes may induce different types of strategic behavior and eventually free riding by the economic agents involved. We conducted a choice experiment through a face-to-face survey held in 2020 for a representative sample of the Portuguese population. We investigate the national population’s WTP to invest in oil spills’ prevention along the coastline of mainland Portugal to ensure the provision of four marine and coastal ecosystem services (MCES): (1) biodiversity conservation, (2) beach use, (3) coastal protection and (4) surf. We used a split-sample design to test for differences between the two PVs considered, a mandatory income tax and a voluntary contribution collected through a crowdfunding campaign. We estimate a mixed logit model (MXL) in WTP-space. Furthermore, we control for several sociodemographic characteristics to capture the influence of respondents’ heterogeneity on the elicited WTP, and to check the robustness of our results. We find that mean WTP estimates are positive and significant for all ES except for surf. Biodiversity conservation has the highest WTP estimate. The results obtained suggest that the lack of trust in institutions, fairness concerns and disbelief in policy consequentiality seem to be intrinsic to the Portuguese population, influencing WTP regardless of the PV. However, when comparing an extra income tax with a crowdfunding campaign, respondents have a lower preference for the status quo in this latter case. Therefore, our results highlight the importance of better understanding the role that the payment vehicle may play in funding ecosystem services’ conservation. This is important since how populations respond to incentives for sustainability purposes is crucial to ensure that the targets are met in a more efficient (or cost-effective) and equitable way.info:eu-repo/semantics/submittedVersio

Renal sympathetic ablation using an irrigated-tip catheter: an attractive option?

BACKGROUND: Catheter-based renal sympathetic denervation has emerged as an adjunct strategy to control refractory hypertension. No studies have yet compared the tissue effects of different catheters, powers and time periods of radiofrequency application, which was the objective of this study. METHODS: Six porcine renal arteries were sectioned in their longitudinal axis and placed in the flow chamber designed to simulate physiological renal flow conditions. The catheters were placed obliquely to the artery with constant contact pressure. Radiofrequency ablations were performed using three different catheters: 4 mm/5 F solid-tip electrode, 4 mm/7 F solid-tip electrode, and open irrigated-tip 4 mm/7 F electrode. Two different powers were used (8 W and 15 W) for 30, 60 and 120 seconds. RESULTS: A total of 18 ablations were performed. More significant nerve damage was observed with the 4 mm/5 F catheter and power of 8 W only when the application duration was extended to 120 seconds. On the other hand, significant nerve damage was observed with the 4 mm/7 F catheter with all power (8 W and 15 W) and duration (30, 60, and 120 seconds) options tested. Deeper lesions were observed with the use of the irrigated catheter, regardless of power and time periods of radiofrequency application. CONCLUSIONS: The irrigated-tip catheters produce deeper lesions than solid-tip catheters and their use might be more beneficial in treating patients with renal sympathetic denervation. The clinical applicability of these results, however, should be confirmed.INTRODUÇÃO: A denervação simpática renal por meio de cateteres surgiu como estratégia adjunta para o controle da hipertensão arterial resistente. Nenhum estudo até o momento comparou os efeitos teciduais de diferentes cateteres, potências e tempos de aplicação da radiofrequência, objetivo do presente estudo. MÉTODOS: Seis artérias renais de porco foram seccionadas em seu eixo longitudinal e colocadas em uma câmara projetada para simular condições fisiológicas de fluxo renal. Os cateteres foram posicionados obliquamente à artéria, mantendo-se pressão de contato constante. Aplicações de radiofrequência foram realizadas utilizando-se três diferentes dispositivos: eletrodo de ponta sólida 4 mm/5 F, eletrodo de ponta sólida 4 mm/7 F, e eletrodo com ponta aberta irrigada 4 mm/7 F. Duas energias foram aplicadas (8 W e 15 W), durante 30 segundos, 60 segundos e 120 segundos. RESULTADOS: No total foram realizadas 18 aplicações. Injúria neural renal mais significativa foi observada utilizando-se cateter 4 mm/5 F e energia de 8 W apenas quando a duração da aplicação foi estendida a 120 segundos. Por outro lado, significante dano neural foi observado com o cateter 4 mm/7 F com todas as potências (8 W e 15 W) e durações testadas (30 segundos, 60 segundos e 120 segundos). Lesões mais profundas foram notadas quando o cateter irrigado foi utilizado, independentemente da potência e da duração da aplicação. CONCLUSÕES: O cateter com ponta irrigada produz lesões mais profundas que os cateteres de ponta sólida e seu uso pode ser mais vantajoso na denervação simpática renal. A aplicabilidade clínica desses resultados, entretanto, deve ser confirmada.Invasiva do Instituto Dante Pazzanese de CardiologiaInstituto Dante Pazzanese de CardiologiaUniversidade Federal de São Paulo (UNIFESP)Universidade Federal de São Paulo (UNIFESP) Departamento de PatologiaUNIFESP, Depto. de PatologiaSciEL

Fiber Supported Droplet Combustion-2 (FSDC-2)

Experimental results for the burning characteristics of fiber supported, liquid droplets in ambient Shuttle cabin air (21% oxygen, 1 bar pressure) were obtained from the Glove Box Facility aboard the STS-94/MSL-1 mission using the Fiber Supported Droplet Combustion - 2 (FSDC-2) apparatus. The combustion of individual droplets of methanol/water mixtures, ethanol, ethanol/water azeotrope, n-heptane, n-decane, and n-heptane/n-hexadecane mixtures were studied in quiescent air. The effects of low velocity, laminar gas phase forced convection on the combustion of individual droplets of n-heptane and n-decane were investigated and interactions of two droplet-arrays of n-heptane and n-decane droplets were also studied with and without gas phase convective flow. Initial diameters ranging from about 2mm to over 6mm were burned on 80-100 micron silicon fibers. In addition to phenomenological observations, quantitative data were obtained in the form of backlit images of the burning droplets, overall flame images, and radiometric combustion emission measurements as a function of the burning time in each experiment. In all, 124 of the 129 attempted experiments (or about twice the number of experiments originally planned for the STS-94/MSL-1 mission) were conducted successfully. The experimental results contribute new observations on the combustion properties of pure alkanes, binary alkane mixtures, and simple alcohols for droplet sizes not studied previously, including measurements on individual droplets and two-droplet arrays, inclusive of the effects of forced gas phase convection. New phenomena characterized experimentally for the first time include radiative extinction of droplet burning for alkanes and the "twin effect" which occurs as a result of interactions during the combustion of two-droplet arrays. Numerical modeling of isolated droplet combustion phenomenon has been conducted for methanol/water mixtures, n-heptane, and n-heptane/n-hexadecane mixtures, and results compare quantitatively with those found experimentally for methanol/water mixtures. Initial computational results qualitatively predict experimental results obtained for isolated n-heptane and n-heptane/n-hexadecane droplet combustion, although the effects of sooting are not yet included in the modeling work. Numerical modeling of ethanol and ethanol/water droplet burning is under development. Considerable data remain to be fully analyzed and will provide a large database for comparisons with further numerical and analytical modeling and development of future free droplet experiments aboard space platforms

TÉCNICA ALTERNATIVA PARA CONTROLE DE SENSIBILIDADE DENTINÁRIA, GERADA PELO CLAREAMENTO DENTÁRIO DE CONSULTÓRIO: uso de óleo ozonizado

Introdução: O uso do Ozônio é descrito na Odontologia como terapia complementar para clareamento dentário, desinfecção do canal radicular, reparo tecidual, ação anti-inflamatória e analgésica. Quando utilizado em associação a óleos naturais, como o óleo de girassol, tem suas propriedades intensificadas e melhor manejo clínico. Objetivos: Retratar clinicamente a utilização de óleo de girassol ozonizado (OGZ) no controle da sensibilidade dentinária, gerada pela ação de géis clareadores. Metodologia: Paciente (A-controle) e (B), 19 anos, sexo masculino e feminino, respectivamente, procuraram atendimento na Faculdade de Odontologia da Universidade Federal de Uberlândia, com queixa estética de seus dentes hígidos, avaliados, pessoalmente, como escurecidos. Após anamnese e exame clínico, foi indicado a técnica do clareamento dentário (CD) de consultório, utilizando peróxido de hidrogênio (HP). Previamente ao clareamento foi realizada profilaxia e após 7 dias os pacientes retornaram para realizar o CD, onde (A) foi submetido ao CD convencional com HP (40 min) e (B) recebeu aplicação prévia de OGZ como dessensibilizante (10 min), seguido de limpeza da superfície dos dentes (gaze) e aplicação de HP. A alteração de cor foi evidenciada por Espectrofotômetro (Easyshade – padrão CieLAB) antes e após os procedimentos, além da sintomatologia dolorosa e sensibilidade dentinária durante e após o CD (24h). Considerações finais: A alteração de cor foi efetiva em ambos os casos e paciente B não apresentou sintomatologia dolorosa durante e após o procedimento clareador. O OGZ aparenta ser efetivo como dessensibilizante de uso prévio ao CD

The Salmonella enterica Serotype Typhimurium Effector Proteins SipA, SopA, SopB, SopD, and SopE2 Act in Concert To Induce Diarrhea in Calves

Salmonella enterica serotype Typhimurium requires a functional type III secretion system encoded by Salmonella pathogenicity island 1 (SPI1) to cause diarrhea. We investigated the role of genes encoding secreted target proteins of the SPI1-associated type III secretion system for enteropathogenicity in calves. Salmonella serotype Typhimurium strains having mutations in sptP, avrA, sspH1, or slrP induced fluid secretion in the bovine ligated ileal loop model at levels similar to that of the wild type. In contrast, mutations in sipA, sopA, sopB, sopD, or sopE2 significantly reduced fluid accumulation in bovine ligated ileal loops at 8 h postinfection. A strain carrying mutations in sipA, sopA, sopB, sopD, and sopE2 (sipA sopABDE2 mutant) caused the same level of fluid accumulation in bovine ligated ileal loops as a strain carrying a mutation in sipB, a SPI1 gene required for the translocation of effector proteins into host cells. A positive correlation was observed between the severity of histopathological lesions detected in the ileal mucosa and the levels of fluid accumulation induced by the different mutants. After oral infection of calves, the Salmonella serotype Typhimurium sipAsopABDE2 mutant caused only mild diarrhea and was more strongly attenuated than strains having only single mutations. These data demonstrate that SipA, SopA, SopB, SopD, and SopE2 are major virulence factors responsible for diarrhea during Salmonella serotype Typhimurium infection of calves

Metabolic reconstitution of germ-free mice by a gnotobiotic microbiota varies over the circadian cycle.

The capacity of the intestinal microbiota to degrade otherwise indigestible diet components is known to greatly improve the recovery of energy from food. This has led to the hypothesis that increased digestive efficiency may underlie the contribution of the microbiota to obesity. OligoMM12-colonized gnotobiotic mice have a consistently higher fat mass than germ-free (GF) or fully colonized counterparts. We therefore investigated their food intake, digestion efficiency, energy expenditure, and respiratory quotient using a novel isolator-housed metabolic cage system, which allows long-term measurements without contamination risk. This demonstrated that microbiota-released calories are perfectly balanced by decreased food intake in fully colonized versus gnotobiotic OligoMM12 and GF mice fed a standard chow diet, i.e., microbiota-released calories can in fact be well integrated into appetite control. We also observed no significant difference in energy expenditure after normalization by lean mass between the different microbiota groups, suggesting that cumulative small differences in energy balance, or altered energy storage, must underlie fat accumulation in OligoMM12 mice. Consistent with altered energy storage, major differences were observed in the type of respiratory substrates used in metabolism over the circadian cycle: In GF mice, the respiratory exchange ratio (RER) was consistently lower than that of fully colonized mice at all times of day, indicative of more reliance on fat and less on glucose metabolism. Intriguingly, the RER of OligoMM12-colonized gnotobiotic mice phenocopied fully colonized mice during the dark (active/eating) phase but phenocopied GF mice during the light (fasting/resting) phase. Further, OligoMM12-colonized mice showed a GF-like drop in liver glycogen storage during the light phase and both liver and plasma metabolomes of OligoMM12 mice clustered closely with GF mice. This implies the existence of microbiota functions that are required to maintain normal host metabolism during the resting/fasting phase of circadian cycle and which are absent in the OligoMM12 consortium