Konjungsi Dalam Lirik Lagu-lagu Kelompok Band Avenged Sevenfold Pada Album Nightmare

This research is entitled “Conjunction in the Songs' lyiric of Avenged Sevenfold Group Band in the album “Nightmare” that is also known as A7x. In the aspect of function, conjunction is divided into coordinative conjunction and subordinative conjunction. The object of this study is to see the conjunctions in the lyirics and see the function and meaning of the conjunctions. The method used is descriptive, and the concepts of Payne (2011), and Quirk and Greenbaum (1999). The result of this research shows that, the coordinate conjunctions found are and, but, or, for, so and still. Meanwhile the subordinate conjunctions found are where, when, that, before, how, as long as, as soon as, since, if, because, while, until, than, once, and as. Coordinate conjunctions connect words, phrases, clauses, and sentences. Meanwhile subordinate conjunctions connect clauses as in the concept. Besides, the subordinate conjunctions found also connect phrase and clause, and clause and sentence where these two things are not appropriate with the concept used, and the meaning of the conjunctions are fit to the concept

Characterizing Search Behavior in Productivity Software

Complex software applications expose hundreds of commands to users through intricate menu hierarchies. One of the most popular productivity software suites, Microsoft Office, has recently developed functionality that allows users to issue free-form text queries to a search system to quickly find commands they want to execute, retrieve help documentation or access web results in a unified interface. In this paper, we analyze millions of search sessions originating from within Microsoft Office applications, collected over one month of activity, in an effort to characterize search behavior in productivity software. Our research brings together previous efforts in analyzing command usage in large-scale applications and efforts in understanding search behavior in environments other than the web. Our findings show that users engage primarily in command search, and that re-accessing commands through search is a frequent behavior. Our work represents the first large-scale analysis of search over command spaces and is an important first step in understanding how search systems integrated with productivity software can be successfully developed

<i>P. berghei</i> telomerase subunit TERT is essential for parasite survival

Telomeres define the ends of chromosomes protecting eukaryotic cells from chromosome instability and eventual cell death. The complex regulation of telomeres involves various proteins including telomerase, which is a specialized ribonucleoprotein responsible for telomere maintenance. Telomeres of chromosomes of malaria parasites are kept at a constant length during blood stage proliferation. The 7-bp telomere repeat sequence is universal across different Plasmodium species (GGGTTT/CA), though the average telomere length varies. The catalytic subunit of telomerase, telomerase reverse transcriptase (TERT), is present in all sequenced Plasmodium species and is approximately three times larger than other eukaryotic TERTs. The Plasmodium RNA component of TERT has recently been identified in silico. A strategy to delete the gene encoding TERT via double cross-over (DXO) homologous recombination was undertaken to study the telomerase function in P. berghei. Expression of both TERT and the RNA component (TR) in P. berghei blood stages was analysed by Western blotting and Northern analysis. Average telomere length was measured in several Plasmodium species using Telomere Restriction Fragment (TRF) analysis. TERT and TR were detected in blood stages and an average telomere length of ~950 bp established. Deletion of the tert gene was performed using standard transfection methodologies and we show the presence of tert− mutants in the transfected parasite populations. Cloning of tert- mutants has been attempted multiple times without success. Thorough analysis of the transfected parasite populations and the parasite obtained from extensive parasite cloning from these populations provide evidence for a so called delayed death phenotype as observed in different organisms lacking TERT. The findings indicate that TERT is essential for P. berghei cell survival. The study extends our current knowledge on telomere biology in malaria parasites and validates further investigations to identify telomerase inhibitors to induce parasite cell death

Active-Site-Directed Inhibitors of Prolyl Oligopeptidase Abolish Its Conformational Dynamics

Deciphering conformational dynamics is crucial for understanding the biological functions of proteins and for designing compounds targeting them. In particular, providing an accurate description of microsecond–millisecond motions opens the opportunity for regulating protein–protein interactions (PPIs) by modulating the dynamics of one interacting partner. Here we analyzed the conformational dynamics of prolyl oligopeptidase (POP) and the effects of active-site-directed inhibitors on the dynamics. We used an integrated structural biology approach based on NMR spectroscopy and SAXS experiments complemented by MD simulations. We found that POP is in a slow equilibrium in solution between open and closed conformations, and that inhibitors effectively abolished this equilibrium by stabilizing the enzyme in the closed conformation.This work was supported by the Institute for Research in Biomedicine, MINECO-FEDER (Bio2013-40716-R, CTQ2013-48287 and CTQ2012-32183/BQU), and the Generalitat de Catalunya (XRB and Grup Consolidat 2014SGR521). AL has received funding from the Instituto de Salud Carlos III. PB acknowledges the Agence Nationale de la Recherche (SPINHD-ANR-CHEX-2011) and the ATIP-Avenir program for financial support. FHT’s fellowship is co-funded by the INSERM and the University of Copenhagen. Technical assistance from staff at the P12 beam line (EMBL/DESY) is acknowledged.Peer ReviewedPostprint (author's final draft

Исследователь в современном междисциплинарном пространстве: возможности изучения образов и пространства города советской эпохи

Objectives: The soluble HIV-1 gp120 envelope glycoprotein, after being shed from infected cells, can cross-link its receptors on both HIV-1 infected and non-infected target cells, leading to their activation. We have assessed the impact of soluble gp120 on viral replication in CD4+/CXCR4+ T cells, via its effects on Tat-mediated transactivation of the HIV-1/LTR. Materials and methods: Primary cord blood-derived CD4+/CXCR4+ T cells were stimulated with soluble recombinant gp120 (rgp120) from the HIV-1/HXB2 clone. The level of gene or protein expression was assessed by serial analysis gene expression (SAGE), reverse transcriptase-polymerase chain reaction, western blotting or flow-cytometry analysis. Cellular division of rgp120-stimulated T cells was assessed by CFDA-SE labeling. Long terminal repeat (LTR) activity and HIV infection level were respectively measured by a chemiluminescent P-gal Reporter Gene Assay and by p24 determination. Results: We have demonstrated that rgp120 activates both PKC epsilon and its upstream effector P13K/Akt, involved in the HIV-1 replication process. Moreover, rgp120 enhances the gene, as well as protein expression of the cellular Tat cofactors Tat-Sf1 and SPT5 in primary CD4+/CXCR4+ T cells. Finally, stimulation of HIV-1 infected T cells with rgp120 was found to result in both a higher LTR-activity and an increased production of viral particles. Conclusion: Taken together, these results show that soluble gp120 contributes to HIV-1 replication and dissemination, via the activation of multiple cell signaling pathways and the induction of Tat-cofactor expression, underscoring its potential as a therapeutic target in HIV-1-mediated pathogenesis. (c) 2005 Lippincott Williams and Wilkins

External financing of business from the point of view of entrepreneurs

The SME sector constitutes the vast majority of enterprises in Poland. The main goals of the company are to obtain the highest possible profit and maximize the company’s market value. Often, the owners, despite a good business idea and their own resources, are not able to achieve satisfactory income, which is why the ability to obtain financing for their activity is so important. The research method was a questionnaire, which was designed to present the profile of activity, identify the type of funding, and determine whether obtaining it improved its functioning

The Rising Role of Private Equity in Professional Sports. A Review

Introduction Since 2019, private equity (PE) investment in sports franchises, media rights, and stadium infrastructure has grown significantly due to league bylaw changes. These assets attract PE firms with their strong returns and diversification benefits, especially in economic uncertainty. PE provides long-term value through franchise ownership, media rights monetization, and infrastructure investments. However, challenges include limited governance control and illiquid assets, complicating exit strategies. Despite this, PE remains a growing and appealing sector for investment. Materials and Methods A literature review was conducted using sources like the IMF eLibrary and PubMed, focusing on studies from 2019–2024. Keywords included “private equity,” “sport,” and “investment.” Criteria included analyses of sports financing trends and investment impacts on global markets. Results PE in sports grows yearly due to strong financial returns and diversification benefits. Challenges include governance limits and illiquidity, but PE provides liquidity and operational expertise to underfunded franchises, maximizing revenues and valuations. This aligns sports investments with macroeconomic strategies, boosting their market appeal. Conclusions Private equity's rise in sports marks a shift in financing since 2019, offering liquidity, expertise, and valuation growth. While governance challenges exist, PE investments address underfunding and position themselves as vital players in the financial evolution of professional sports

Sytuacje pracy jako podstawa dla rozwoju kompetencji pracowników branży budowlanej

Celem artykułu jest prezentacja wyników prac badawczych i wdrożeniowych zrealizowanych przez międzynarodowe partnerstwo projektu RenovUp. Podjęto w nim bardzo aktualny temat dotyczący zmian na rynku pracy wynikających z zaostrzających się kryteriów środowiskowych w zakresie efektywności energetycznej budynków oraz rosnącej w związku z tym roli prac renowacyjnych (remontowych). Nowe oczekiwania i wyzwania natury technicznej, prawnej i organizacyjnej stoją przed pracownikami branży budowlanej. Jak wykazały badania międzynarodowego partnerstwa projektu RenovUp, luka kompetencyjna wśród pracowników budowlanych specjalizujących się w pracach remontowych, wykazuje pewną specyfikę. W opinii pracodawców i ekspertów branży, specyfika ta nie jest w wystarczającym stopniu uwzględniania w aktualnej ofercie szkoleniowej. Podjęto zatem działania mające na celu wsparcie procesów rozwoju zawodowego pracowników w oparciu o ich potrzeby wynikające z rzeczywistych sytuacji pracy obserwowanych bezpośrednio na placach budowy. Opracowano i przetestowano (w pięciu krajach) model wsparcia wraz z narzędziami mającymi na celu ułatwić nauczycielom i szkoleniowcom proces obserwowania sytuacji pracy na stanowiskach pracy oraz wnioskowania na ich podstawie i wykorzystywania wyników obserwacji w projektowaniu i wdrażaniu zindywidualizowanych ścieżek edukacyjnych. Artykuł prezentuje założenia modelu wsparcia dla nauczycieli i trenerów branży budowlanej, proponowane narzędzia oraz wyniki ich eksperymentalnego testowania w kilku krajac