37 research outputs found

    Effects of surface thermal forcing on stratified flow past an isolated obstacle

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    The present study investigates basic aspects of the flow of a density-stratified fluid past three-dimensional obstacles for Froude number ~ O(1) and isolated surface thermal forcing representative of diurnally varying mesoscale flows past mountainous islands such as Hawaii. In order to minimize parameter space, we have excluded the effects of friction, rotation, nonuniform ambient flow, and the complexities of realistic surface boundary layer and terrain. Through simple scaling arguments, we deduce that the parameter [eta][superscript]* [[eta][superscript]*≡L[superscript]*[macron] Q[over] Uh≤ft([partial][macron][theta][over][partial] z)[superscript]-1 ~ O(1) for mesoscale flows]controls thermally forced flows for a given Froude number, and we provide crude estimates of a flow response for a range of [eta][superscript]*. The principal question addressed is for what values of [eta][superscript]* will a transition occur from the low-Froude-number flow regime, characterized by the stagnation and splitting of the lower upwind flow, to the regime in which flow passes over rather than around the obstacle. We show that the linear theory captures such a tendency consistently with simple scaling arguments. To provide quantitative measures of flow variability with the Froude number and [eta][superscript]*, we employ an efficient isentropic numerical code and summarize the results of numerous simulations in the form of a regime diagram. The principal result is a simple criterion for the transition of a heated flow from the blocked to unblocked flow regime. We illustrate the relevance of the idealized study to natural flows with an example of applications to a flow past the Hawaiian Archipelago

    The Impacts of Dry Dynamic Cores on Asymmetric Hurricane Intensification

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    The article of record as published may be found at http://dx.doi.org/10.1175/JAS-D-16-0055.1The fundamental pathways for tropical cyclone (TC) intensification are explored by considering axisym- metric and asymmetric impulsive thermal perturbations to balanced, TC-like vortices using the dynamic cores of three different nonlinear numerical models. Attempts at reproducing the results of previous work, which used the community WRF Model, revealed a discrepancy with the impacts of purely asymmetric thermal forcing. The current study finds that thermal asymmetries can have an important, largely positive role on the vortex intensification, whereas other studies find that asymmetric impacts are negligible. Analysis of the spectral energetics of each numerical model indicates that the vortex response to asym- metric thermal perturbations is significantly damped in WRF relative to the other models. Spectral kinetic energy budgets show that this anomalous damping is primarily due to the increased removal of kinetic energy from the vertical divergence of the vertical pressure flux, which is related to the flux of inertia–gravity wave energy. The increased kinetic energy in the other two models is shown to originate around the scales of the heating and propagate upscale with time from nonlinear effects. For very large thermal amplitudes (50 K), the anomalous removal of kinetic energy due to inertia–gravity wave activity is much smaller, resulting in good agreement between models. The results of this paper indicate that the numerical treatment of small-scale processes that project strongly onto inertia–gravity wave energy can lead to significant differences in asymmetric TC intensification. Sensitivity tests with different time integration schemes suggest that diffusion entering into the implicit solution procedure is partly responsible for the anomalous damping of energy.Institute of Geophysics, Planetary Physics and Signatures (IGPPS) at Los Alamos National LaboratoryOffice of Naval Research through program element PE-0602435Institute of Geophysics, Planetary Physics and Signatures (IGPPS) at Los Alamos National LaboratoryOffice of Naval Research through program element PE-060243

    High-Yield Expression of Heterologous [FeFe] Hydrogenases in Escherichia coli

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    BACKGROUND: The realization of hydrogenase-based technologies for renewable H(2) production is presently limited by the need for scalable and high-yielding methods to supply active hydrogenases and their required maturases. PRINCIPAL FINDINGS: In this report, we describe an improved Escherichia coli-based expression system capable of producing 8-30 mg of purified, active [FeFe] hydrogenase per liter of culture, volumetric yields at least 10-fold greater than previously reported. Specifically, we overcame two problems associated with other in vivo production methods: low protein yields and ineffective hydrogenase maturation. The addition of glucose to the growth medium enhances anaerobic metabolism and growth during hydrogenase expression, which substantially increases total yields. Also, we combine iron and cysteine supplementation with the use of an E. coli strain upregulated for iron-sulfur cluster protein accumulation. These measures dramatically improve in vivo hydrogenase activation. Two hydrogenases, HydA1 from Chlamydomonas reinhardtii and HydA (CpI) from Clostridium pasteurianum, were produced with this improved system and subsequently purified. Biophysical characterization and FTIR spectroscopic analysis of these enzymes indicate that they harbor the H-cluster and catalyze H(2) evolution with rates comparable to those of enzymes isolated from their respective native organisms. SIGNIFICANCE: The production system we describe will facilitate basic hydrogenase investigations as well as the development of new technologies that utilize these prolific H(2)-producing enzymes. These methods can also be extended for producing and studying a variety of oxygen-sensitive iron-sulfur proteins as well as other proteins requiring anoxic environments

    Levels of depression in transgender people and its predictors: results of a large matched control study with transgender people accessing clinical services

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    Background: Depression is a serious disorder which significantly impacts wellbeing and quality of life. Studies exploring mental wellbeing in the transgender population are mostly limited by small, non-homogenous samples and lack of matched controls. This study aimed to address these limitations and explore depression rates in a large sample of transgender people, compared with matched controls from the general population, as well as factors predicting depression in those taking cross-sex hormone treatment (CHT) compared to those not. Methods: Transgender individuals (n=913) completed a measure of depression, measures which predict psychopathology (self-esteem, victimization, social support, interpersonal problems), and information regarding CHT use. Participants were matched by age and experienced gender with adults from the general population who had completed the measure of depression. Results: Individuals were categorized as having no, possible or probable depressive disorder. Transgender individuals not on CHT had a nearly four-fold increased risk of probable depressive disorder, compared to controls. Older age, lower self-esteem, poorer interpersonal function and less social support predicted depressive disorder. Use of CHT was associated with less depression. Limitations: Participants were attending a national gender identity service and therefore represent only a sub-group of transgender people. Due to the cross-sectional design, longitudinal research is required to fully confirm the finding that CHT use reduces depression. Conclusion: This study confirms that non-treated transgender individuals have an increased risk of a depressive disorder. Interventions offered alongside gender affirming treatment to develop interpersonal skills, increase self-esteem and improve social support may reduce depression and prepare individuals for a more successful transition
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