Vinculação crianças em creche

Esta investigação tem como objectivo principal estudar o papel da vinculação da criança à creche, desde os seis aos trinta e seis meses de idade, isto é, aquando da sua entrada para a creche. Para tal, e com recurso a uma metodologia qualitativa, desenvolveram-se dois questionários, aplicados a pais e funcionários, e uma checklist de observação, que abrangeu 10 crianças, em momentos específicos como a chegada e a partida da criança.Este estudo permite concluir que a vinculação da criança em creche é fundamental para o seu desenvolvimento salutar, uma vez que quanto mais segura se sentir a criança mais fácil será a sua adaptação. Neste processo, quer os pais quer os profissionais, com maior relevância os educadores, desempenham um papel preponderante.This research has as main aim studying the role of children´s tie to Nursery School, since the six to thirty six months of age, meaning, when they begin their school life. Therefore and using a quality methodology, we carry out two surveys applied to Parents and Nursery School Employees and an observation checklist, which included ten children in specific moments, as the children´s arrival and the children´s departure. This study allows concluding that the children´s tie to Nursery School is of the utmost importance to their healthy development, because as more secure they feel, easier will be their adaptation. In this process, Parents as much as Professionals, mainly, Nursery School Teachers play a determinant role

AXL as a modulator of sunitinib response in glioblastoma cell lines

Receptor tyrosine kinase (RTK) targeted therapy has been explored for glioblastoma treatment. However, it is unclear which RTK inhibitors are the most effective and there are no predictive biomarkers available. We recently identified the RTK AXL as a putative target for the pan-RTK inhibitors cediranib and sunitinib, which are under clinical trials for glioblastoma patients. Here, we provide evidence that AXL activity can modulate sunitinib response in glioblastoma cell lines. We found that AXL knockdown conferred lower sensitivity to sunitinib by rescuing migratory defects and inhibiting apoptosis in cells expressing high AXL basal levels. Accordingly, overactivation of AXL by its ligand GAS6 rendered AXL positive glioblastoma cells more sensitive to sunitinib. AXL knockdown induced a cellular rewiring of several growth signaling pathways through activation of RTKs, such as EGFR, as well as intracellular pathways such as MAPK and AKT. The combination of sunitinib with a specific AKT inhibitor reverted the resistance of AXL-silenced cells to sunitinib. Together, our results suggest that sunitinib inhibits AXL and AXL activation status modulates therapy response of glioblastoma cells to sunitinib. Moreover, it indicates that combining sunitinib therapy with AKT pathway inhibitors could overcome sunitinib resistance.This work was funded by Fundacao para a Ciencia e Tecnologia (FCT), Portugal (project: PTDC/SAU-TOX/114549/2009). Olga Martinho is a recipient of a Post-Doc fellowship (UMINHO/BPD/32/2013) from QREN. We would like to acknowledge Dr. Shuang-En Chuang from the National Health Research Institute, Taiwan, for providing AXL vectors, and Dr. Raquel Andrade for critical review of the manuscript

Parental Knowledge on Breastfeeding: Contributions to a Clinical Supervision Model in Nursing

Abstract—Parental skills development in breastfeeding, not only eases the bonding and the maintenance of the process, but also, mirrors the excellence of nursing care. The study aims to assess parental knowledge concerning breastfeeding, in order to bring forth contributions for a clinical supervision model, which promotes the development of nursing skills.A quantitative and cross sectional study was conducted on a non-probability sample consisting of 135 recent mothers. The enquiry form applied allowed the assessment of parental skills on breastfeeding and in determining the conditions for the development of these skills. Most women (63%) provided information on the benefits of breastfeeding, 75% of the mothers did not show familiarity with strategies on how to continue breastfeeding. The study pointed to the existence of practices that impair the progress of acknowledgement on breastfeeding and contribute to early withdrawal, such as: delay in breastfeeding initiation, early introduction to infant formula, lack of nurses’ guidance and support on breastfeeding, during the post-partum period. Clinical supervision can be a means for the development of nursing skills allowing a widespread improvement of practices and rates (exclusivity and duration) of breastfeeding, boosting parent’s capacity, security, trust and satisfaction, which regards breastfeeding.info:eu-repo/semantics/publishedVersio

Entre a Natureza e o Homem : o extremo do vínculo geométrico

Dissertação de mestrado integrado em Arquitectura - Cultura ArquitectónicaEntre a Natureza e o Homem é um ensaio sobre a essência da arquitectura enquanto vínculo natural e humano. Pretendendo o enlace da Natureza e do Homem, incide-se no revigorar da arquitectura, através da percepção humana das “forças em espirro” e “forças exactas”, concretizando um extremo geométrico que coarcta os protagonistas no trabalho comummente laborado. Na percepção da complexidade dos intervenientes, a conquista pelo objectivo adivinha-se extremosa pela prática. Assim, desenvolve-se uma proposta protótipo, capaz de albergar Natureza e Homem, num momento realçado no caos e na beleza e, quantificado em instintos naturais e razões humanas que transparecem em referências alusivas para a finalidade. Neste sentido, a formalização de uma base arquitectónica assenta num desenho experimental de uma geometria humana, embrionária na Natureza. Aproveitando as causas naturais para a racionalidade humana, transforma-se no vínculo que evidencia a essência geométrica. A Natureza é a harmonia que outorga vida embrionária à matéria. O Homem é a razão para o extremo geométrico da relação. A matéria é geometrizada no trabalho das forças que, Natureza e Homem, acenam pelo diálogo que se mostra terno e sincero na essência da arquitectura. Em suma, Entre a Natureza e o Homem: o extremo do vínculo geométrico procura o limite de uma geometria num momento específico, onde os protagonistas comunicam uma relação sincera, para evidenciar a essência da arquitectura, enquanto “agrafo” entre Natureza e Homem. Mais do que Natureza; mais do que Homem; a geometria é o extremo de um vínculo que supera os dois, na conexão que lhes garante, superando-se na sua essência.Between Nature and Man is an essay about the essence of architecture as a natural and a human bond. Intending to link Nature and Man, it focuses on the invigoration of architecture, through the human perception of “sneeze forces” and “exact forces”, materializing in a geometrical extreme that curtails the protagonists in the commonly labored work. In the consciousness of the characters’ complexity, the conquest of the objective appears unwearying by practice. Thus a prototype proposal is developed, capable of housing both Nature and Man, in a moment driven by chaos and beauty and quantified in natural instincts and human reasoning, which are ghosted in allusive-to-goal references. In this sense, the formalization of an architectural basis leans on the experimental design of a human, embryonic of Nature type of geometry. The natural causes being taken into consideration for the human rationality, it transforms into the bond that highlights the geometrical essence. Nature is the harmony that bestows embryonic life to matter. Man is the reason for the geometrical extreme of this relation. The matter is turned into geometry through the working process of the forces, to which Nature and Man refer via their dialogue, tender and sincere in the essence of architecture. In short, Between Nature and Man: the extreme of the geometrical bond seeks the limit of a given geometry in a specific moment, wherein the protagonists speak a sincere connection, in order to highlight the essence of the architecture whilst the “staple” between Nature and Man. Beyond Nature; beyond Man; the geometry is the extreme of a bond that overcomes both, in the connection granted to them, thus overcoming its own essence

Protein Kinase WNK2 has a Tumour Suppressor Role in Gliomas

Malignant glioblastoma is the most common and lethal adult brain tumour type. Recently, the promoter region of the protein kinaseWNK2 gene was found to be hypermethylated in 29 of 31 infiltrative gliomas and about 5 of 7 meningiomas. We have previously described that theexperimental depletion of WNK2 expression decreases RhoA activity whilst leading to increased Rac1 activity. RhoA/Rac1 activities are important forcell migration and glioblastomas are very invasive tumours so that we tested the effects of WNK2 on wound-healing assays in glioma cell lines SW1088and A172. SW1088 cells express endogenous WNK2 and we observed that wound closure was increased upon experimental depletion of endogenousWNK2. In contrast, A172 cells display complete promoter region methylation and WNK2 re-expression was found to decrease migration. Consistently,we observed an increase in Rac1 activity in SW1088 cells upon WNK2 down-regulation, but lower levels of active Rac1 in A172 cells stably expressingWNK2 cDNA when compared with an equivalent cell line stably transfected with the same empty vector. Our studies indicate that loss of WNK2expression promotes Rac1 activation and may contribute to the highly invasive phenotype that glioblastomas present.Fundação para a Ciência e Tecnologi

ASPM is an oncoprotein and activates EGFR

Este resumo faz parte de: Book of abstracts of the Meeting of the Institute for Biotechnology and Bioengineering, 2, Braga, Portugal, 2010. A versão completa do livro de atas está disponível em: http://hdl.handle.net/1822/1096

Comparative assessment of infiltration, runoff and erosion sprinkler irrigated soils

Abstract The impacts of sprinkler irrigation on infiltration, runoff and sediment loss of ten representative soils of Southern Portugal were assessed by laboratory sprinkler irrigation simulation tests. All soils showed very low permeability to applied water. The mechanical impact of water droplets enhanced soil dispersion and further lowered their infiltration capacity, particularly for high clay plus silt content soils that showed the poorest results. As a consequence, high runoff and sediment losses were also measured, primarily with the first irrigation. More moderate losses were observed thereafter. Soils with higher sand particle size fractions better absorbed the energy impact of droplets and showed higher infiltration rates and lower runoff and sediment losses. Polyacrylamide (PAM) applied to the soils through the irrigation water acted as a binding and settling agent to increase soils aggregate stability and infiltration and reduce runoff and sediment losses. Slope increase, from 2·5 to 5%, decreased overall soils infiltration by 7% and increased runoff and sediment losses by 10 and 27%, respectively. Exposed to the same change in slope, PAM application boosted overall infiltration of treated soils to a 24% difference and increased runoff by only 10%. It had a less positive effect on sediment loss, the 5% slope being responsible for a 52% increase. In agreement with this the tests showed that, compared to the control, exposure of PAM-treated soil on 2·5 and 5% slopes enhanced overall infiltration to 457 and 642% respectively, reduced runoff by 25% on both cases and lessened sediment loss by 39 and 27%. The demonstrated ability of PAM to influence surface soil conditions of specific soils can be used to reduce the environmental risks associated with the intensive use of sprinkler irrigation in Southern Portugal. It offers a safe, practical and non-intrusive management alternative to current costly, labour- and energy-intensive practices of increasing the number of machine turns and building storage basins to control runoff and soil erosion

O pastoreio extensivo no Campo Branco. Sustentabilidade sócio-económica e interligação com os objectivos de conservação d

Mestrado em Engenharia Agronómica - Economia Agrária e Gestão do Território - Instituto Superior de AgronomiaDuring centurys it was developted one extensive grazing system in the Campo Branco region, contributing to the evolution of a landscape called steppic fields. The habitats in this region are classified as proritary under the nature conservation point of view. During the LACOPE project (Large Scale Extensive Grazing Systems in Europe) it was developed a linear programming model that simulated several agrarian political cenarys and how these measures influenced the land uses, economic sustainability of the farms and the survival of the habitats. In this work we present the results of the modelling process and the several political measures, agricultural and others, that influenced and modelated the landscape since the XIX Century. The Zonal Plan of Castro Verde was one of the few measures that have succeed to maintain some economic sustainability and the nature conservation purposes. According with the modelization process and bibliography the grazing system is not sustainable but is essential for the maintenance of the steppic birds. We evaluate possible measures that can be implemented in a way that allows the survival of the extensive grazing farms and the habitats

Análise das estratégias pedagógicas adoptadas na formação profissional online

Tese de mestrado, Ciências da Educação (Tecnologias Educativas), Universidade de Lisboa, Instituto de Educação, 2010Numa sociedade em que é cada vez mais visível o recurso à formação profissional online, parece ser também crescente a preocupação em perceber como os processos de ensino e de aprendizagem online que poderão responder eficazmente às necessidades específicas dos formandos. Neste sentido, desenvolvemos este estudo visando compreender quais as estratégias pedagógicas de formação online mais adequadas a uma aprendizagem efectiva dos formandos. Atendendo à abrangência da problemática, optámos pela metodologia de estudo de caso, tendo realizado o estudo numa Empresa que desenvolve a sua actividade na área da Formação Profissional (entre outras), através da realização de (1) entrevistas aos tutores dos cursos online e à responsável pela área de e-learning, (2) questionários aos formandos que frequentaram a formação online nesta empresa, (3) análises documentais e (4) conversas informais com a gestora do LMS e da equipa de tutoria. Apesar de algumas limitações que acompanharam a realização do estudo, as conclusões alcançadas apontam no sentido de que as estratégias pedagógicas que mais eficazmente contribuem para o envolvimento dos formandos no processo de aprendizagem são (1) a eficaz estruturação e concepção de conteúdos e de cursos ministrados online, (2) o acompanhamento e feedback fornecidos pelo tutor, (3) o desenvolvimento de actividades práticas, (4) os processos de interacção entre formandos, tutor e conteúdos e (5) as formas de avaliação, essencialmente, com objectivos formativos e de auto-diagnóstico.In a society that is increasingly turning to online training, there also seems to be a growing concern about the online teaching and learning processes that respond to the needs of learners more effectively. We, therefore, developed this study in order to understand which teaching strategies used on online learning are most likely to promote effective learning by the trainees. Considering the complex nature of this subject, we decided to use the case study methodology. The study was carried out in an organization that operates in the Professional Training area (amongst others), by (1) conducting interviews with the tutors of online courses and with the person in charge of the e-learning area, (2) applying questionnaires to trainees who participated on online training courses with this organization, (3) using documental reviews as well as (4) informal conversations with the LMS manager and the tutors‟ team manager. Despite some limitations that occurred during the time the study was conducted, the conclusions we reached, seem to point that the educational strategies that contribute most effectively to the involvement of students in the learning process are (1) the effective structuring and design of online contents and courses, (2) the monitoring and feedback provided by the tutor, (3) the opportunity of carrying out practical activities, (4) the interaction processes between students, tutors and content and (5) the forms of assessment, namely, with formative and self-diagnosis objectives