Employee and partner surveys wave 3 of the Linked-Employer-Employee-Panel (LEEP-B3) Project (DFG – 373090005): Organizational Inequalities and Interdependencies between Capabilities in Work and Personal Life: A Study of Employees in Different Work Organizations. Technical Report

Marx C, Abendroth A, Bächmann A-C, et al. Employee and partner surveys wave 3 of the Linked-Employer-Employee-Panel (LEEP-B3) Project (DFG – 373090005): Organizational Inequalities and Interdependencies between Capabilities in Work and Personal Life: A Study of Employees in Different Work Organizations. Technical Report. Bielefeld: Univ., Fak. für Soziologie; 2020

Methodenbericht Faktorieller Survey (Vignetten) Projekt B3 „Wechselwirkungen zwischen Verwirklichungschancen im Berufs- und Privatleben“

Reimann M, Andernach B, Schunck R, et al. Methodenbericht Faktorieller Survey (Vignetten) Projekt B3 „Wechselwirkungen zwischen Verwirklichungschancen im Berufs- und Privatleben“. SFB 882 Technical Report Series. Vol 9. Bielefeld: DFG Research Center (SFB) 882 From Heterogeneities to Inequalities; 2014

Technical Report Factorial Survey (Vignettes) Wave 1: Project B3 “Interactions Between Capabilities in Work and Private Life”

Reimann M, Andernach B, Schunck R, et al. Technical Report Factorial Survey (Vignettes) Wave 1: Project B3 “Interactions Between Capabilities in Work and Private Life”. SFB 882 Technical Report Series. Vol 20. Bielefeld: DFG Research Center (SFB) 882 From Heterogeneities to Inequalities; 2015

Technical report employer survey wave 2: Project B3 "Interactions Between Capabilities in Work and Private Life"

Reimann M, Pausch S, Diewald M, et al. Technical report employer survey wave 2: Project B3 "Interactions Between Capabilities in Work and Private Life". SFB 882 Technical Report Series. Vol 19. Bielefeld: DFG Research Center (SFB) 882 From Heterogeneities to Inequalities; 2015

Update on the diagnosis of tuberculosis

Background Tuberculosis remains a global public health threat, and the development of rapid and precise diagnostic tools is the key to enabling the early start of treatment, monitoring response to treatment, and preventing the spread of the disease. Objective An overview of recent progress in host- and pathogen-based tuberculosis diagnostics. Sources We conducted a PubMed search of recent relevant articles and guidelines on tuberculosis screening and diagnosis. Content An overview of currently used methods and perspectives in the following areas of tuberculosis diagnostics is provided: immune-based diagnostics, X-ray, clinical symptoms and scores, cough detection, culture of Mycobacterium tuberculosis and identifying its resistance profile using phenotypic and genotypic methods, including next generation sequencing, sputum- and non-sputum-based molecular diagnosis of tuberculosis and monitoring of response to treatment. Implications A brief overview of the most relevant advances and changes in international guidelines regarding screening and diagnosing tuberculosis is provided in this review. It aims at reviewing all relevant areas of diagnostics, including both pathogen- and host-based methods.PostprintPeer reviewe

Verknüpfung der LEEP-B3 Befragungsdaten mit administrativen IAB-Daten

Jacobebbinghaus P, Seth S, Diewald M, et al. Verknüpfung der LEEP-B3 Befragungsdaten mit administrativen IAB-Daten. SFB 882 Technical Report Series. Vol 14. Bielefeld: DFG Research Center (SFB) 882 From Heterogeneities to Inequalities; 2014

Linking LEEP-B3 Survey Data with Administrative IAB Data

Jacobebbinghaus P, Seth S, Diewald M, et al. Linking LEEP-B3 Survey Data with Administrative IAB Data. SFB 882 Technical Report Series. Vol 21. Bielefeld: DFG Research Center (SFB) 882 From Heterogeneities to Inequalities; 2015

Tuberculostearic Acid-Containing Phosphatidylinositols as Markers of Bacterial Burden in Tuberculosis

One-fourth of the global human population is estimated to be infected with strains of the Mycobacterium tuberculosis complex (MTBC), the causative agent of tuberculosis (TB). Using lipidomic approaches, we show that tuberculostearic acid (TSA)-containing phosphatidylinositols (PIs) are molecular markers for infection with clinically relevant MTBC strains and signify bacterial burden. For the most abundant lipid marker, detection limits of ∼10$^{2}$ colony forming units (CFUs) and ∼10$^{3}$ CFUs for bacterial and cell culture systems were determined, respectively. We developed a targeted lipid assay, which can be performed within a day including sample preparation─roughly 30-fold faster than in conventional methods based on bacterial culture. This indirect and culture-free detection approach allowed us to determine pathogen loads in infected murine macrophages, human neutrophils, and murine lung tissue. These marker lipids inferred from mycobacterial PIs were found in higher levels in peripheral blood mononuclear cells of TB patients compared to healthy individuals. Moreover, in a small cohort of drug-susceptible TB patients, elevated levels of these molecular markers were detected at the start of therapy and declined upon successful anti-TB treatment. Thus, the concentration of TSA-containing PIs can be used as a correlate for the mycobacterial burden in experimental models and in vitro systems and may prospectively also provide a clinically relevant tool to monitor TB severity

Perspectives for systems biology in the management of tuberculosis

Standardised management of tuberculosis may soon be replaced by individualised, precision medicine-guided therapies informed with knowledge provided by the field of systems biology. Systems biology is a rapidly expanding field of computational and mathematical analysis and modelling of complex biological systems that can provide insights into mechanisms underlying tuberculosis, identify novel biomarkers, and help to optimise prevention, diagnosis and treatment of disease. These advances are critically important in the context of the evolving epidemic of drug-resistant tuberculosis. Here, we review the available evidence on the role of systems biology approaches - human and mycobacterial genomics and transcriptomics, proteomics, lipidomics/metabolomics, immunophenotyping, systems pharmacology and gut microbiomes - in the management of tuberculosis including prediction of risk for disease progression, severity of mycobacterial virulence and drug resistance, adverse events, comorbidities, response to therapy and treatment outcomes. Application of the Grading of Recommendations, Assessment, Development and Evaluation (GRADE) approach demonstrated that at present most of the studies provide "very low" certainty of evidence for answering clinically relevant questions. Further studies in large prospective cohorts of patients, including randomised clinical trials, are necessary to assess the applicability of the findings in tuberculosis prevention and more efficient clinical management of patients.Publisher PDFPeer reviewe