37 research outputs found

    Economia de comunhão na liberdade promovendo o desenvolvimento local sustentável: cultura, gestão e valores

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    Este trabalho traz o resultado da pesquisa realizada para identificar se e como práticas das organizações de Economia de Comunhão na Liberdade contribuem para promover uma cultura organizacional que possa contribuir no desenvolvimento local sustentável. A coleta de dados foi realizada a partir de pesquisa de campo quali-quantitativa com a aplicação de questionário fechado e entrevistas semiestruturadas para gestores e funcionários, e observação não participante. Os dados levantados e os resultados obtidos indicam que, mesmo tendo o propósito de partilharem o lucro e incidirem na sociedade de modo direto no espírito da Fraternidade, as empresas pesquisadas têm diferentes patamares de influência e, em alguns casos, é possível afirmar que esta influência é ainda pouco perceptível para a sociedade em geral

    Role of Immunohistochemistry in the Diagnosis of Leptospirosis in Neotropical Primates

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    Background: Leptospirosis is considered the most widespread zoonosis worldwide, occurring more frequently in tropical and developing regions. The aim of the present study was to detect the presence of Leptospira spp. in different primate tissues, using immunohistochemical (IHC) assays, taking advantage of the considerable number of necropsies compatible with a diagnosis of leptospirosis in neotropical primates at the Animal Pathology Laboratory (APL) of the University of Passo Fundo (UPF) in the northern region of Rio Grande do Sul.Materials, Methods & Results: Paraffin-embedded primate tissue samples were selected from necropsy examinations and subjected to IHC. The streptavidin-biotin-peroxidase method was used with diaminobenzidine chromogen (DAB) to verify immunostaining. Of the101 primates tested for Leptospira spp., 51.48% were positive; taining was distributed between lung (76.92%), liver (44.23%), and kidney (32.69%) tissue. Analysis of the combined anatomopathological verification data of the studied organs revealed a high frequency of lesions commonly observed in the tissues of animals exposed to the pathogen. For complementary diagnosis, an anti-Leptospira spp. antibody test was performed in primates at the UPF-Zoo, from which a population of the necropsied animals originated. The microscopic agglutination test (MAT) was utilized, which demonstrated 90.47% positivity in 21 individuals; sejroe and panama were the most frequent serovars.Discussion: Different intensities of tissue immunostaining were observed. Areas of fragmented or diffuse staining were considered to indicate equal positivity to that indicated by areas of staining with preserved morphology. Of 52 Leptospirapositive primates, most presented some degree of staining in lung samples, which shows a high level of involvement for this organ in primate leptospirosis. Conventional pathological diagnostic methods do not allow fort issue antigen recognition, thus making the IHC technique important to facilitate conclusive antigen sample verification. In the liver, leptospires were detected mostly between the sinusoids, hepatocytes, and Kupffer cells. In kidney tissues, staining indicated small agglomerates in the tubular lumen, interstitium, and glomeruli. All these forms of presentation have been previously reported. Considering that we detected the highest number of positive samples in lung tissue, followed by those from liver and kidney tissue, we argue that the IHC technique, when applied to samples of these three tissues, decreases the chance of false negatives. Anatomopathological studies of primate leptospirosis are scarce. In dogs, renal lesions are characterized by the necrosis and degeneration of tubular epithelium, cellular debris, and hyaline cylinders. In the liver, hepatocyte cord dissociation and biliary pigment accumulation within the canaliculi and hepatocellular necrosis are observed. These findings are similar to those from our study. In the lung, diffuse alveolar lesions are reported, with hemorrhage and edema, in addition to capillaritis. The high frequency of Leptospira-positive animals determined by serological examination was consistent with the IHC findings, thus confirming the pathogen’s high prevalence in neotropical primate populations in the studied region. Serological surveys on primate populations have already been carried out and have revealed frequency and serovar variations between regions. Immunohistochemical examination allows the detection of leptospires in various tissues and should be used based on the characteristics of the investigated case

    Trajetórias da Educomunicação nas Políticas Públicas e a Formação de seus Profissionais

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    Esta obra é composta com os trabalhos apresentados no primeiro subtema, TRAJETÓRIA – Educação para a Comunicação como Política pública, nas perspectivas da Educomunicação e da Mídia-Educação, do II Congresso Internacional de Comunicação e Educação. Os artigos pretendem propiciar trocas de informações e produzir reflexões com os leitores sobre os caminhos percorridos, e ainda a percorrer, tendo como meta a expansão e a legitimação das práticas educomunicativas e/ou mídia-educativas como política pública para o atendimento à formação de crianças, adolescentes, jovens e adultos, no Brasil e no mundo

    Economia de comunhão na liberdade promovendo o desenvolvimento local sustentável: cultura, gestão e valores

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    This work brings the results of the research carried out to identify if and how the practices of the Economy of Communion in Freedom organizations contribute to promoting an organizational culture that may contribute to sustainable local development. The data was collected basically through a survey applying a closed questionnaire and semi-structured interviews for managers and employees. The analyses of data collected indicates that, even though they have the purpose of sharing profits and have some social impact fostering Fraternity, the companies surveyed have different levels of contribution and moreover, in some cases, it seems that this influence is still barely perceptible to society in general.Este trabalho traz o resultado da pesquisa realizada para identificar se e como práticas das organizações de Economia de Comunhão na Liberdade contribuem para promover uma cultura organizacional que possa contribuir no desenvolvimento local sustentável. A coleta de dados foi realizada a partir de pesquisa de campo quali-quantitativa com a aplicação de questionário fechado e entrevistas semiestruturadas para gestores e funcionários, e observação não participante. Os dados levantados e os resultados obtidos indicam que, mesmo tendo o propósito de partilharem o lucro e incidirem na sociedade de modo direto no espírito da Fraternidade, as empresas pesquisadas têm diferentes patamares de influência e, em alguns casos, é possível afirmar que esta influência é ainda pouco perceptível para a sociedade em geral. Este trabajo trae el resultado de la investigación realizada para identificar si y como prácticas de las organizaciones de Economía de Comunión en la Libertad contribuyen a promover una cultura organizacional que pueda contribuir en el desarrollo local sostenible. La recolección de datos fue realizada a partir de la investigación de campo cual-cuantitativa con la aplicación de cuestionario cerrado y entrevistas semiestructuradas para gestores y funcionarios, y observación no participante. Los datos obtenidos y los resultados obtenidos indican que, aun teniendo el propósito de compartir el beneficio e incidir en la sociedad de modo directo en el espíritu de la Fraternidad, las empresas encuestadas tienen diferentes patrones de influencia y, en algunos casos, es posible afirmar que esta influencia es todavía poco perceptible para la sociedad en general

    Role of Immunohistochemistry in the Diagnosis of Leptospirosis in Neotropical Primates

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    Background: Leptospirosis is considered the most widespread zoonosis worldwide, occurring more frequently in tropical and developing regions. The aim of the present study was to detect the presence of Leptospira spp. in different primate tissues, using immunohistochemical (IHC) assays, taking advantage of the considerable number of necropsies compatible with a diagnosis of leptospirosis in neotropical primates at the Animal Pathology Laboratory (APL) of the University of Passo Fundo (UPF) in the northern region of Rio Grande do Sul.Materials, Methods & Results: Paraffin-embedded primate tissue samples were selected from necropsy examinations and subjected to IHC. The streptavidin-biotin-peroxidase method was used with diaminobenzidine chromogen (DAB) to verify immunostaining. Of the101 primates tested for Leptospira spp., 51.48% were positive; taining was distributed between lung (76.92%), liver (44.23%), and kidney (32.69%) tissue. Analysis of the combined anatomopathological verification data of the studied organs revealed a high frequency of lesions commonly observed in the tissues of animals exposed to the pathogen. For complementary diagnosis, an anti-Leptospira spp. antibody test was performed in primates at the UPF-Zoo, from which a population of the necropsied animals originated. The microscopic agglutination test (MAT) was utilized, which demonstrated 90.47% positivity in 21 individuals; sejroe and panama were the most frequent serovars.Discussion: Different intensities of tissue immunostaining were observed. Areas of fragmented or diffuse staining were considered to indicate equal positivity to that indicated by areas of staining with preserved morphology. Of 52 Leptospirapositive primates, most presented some degree of staining in lung samples, which shows a high level of involvement for this organ in primate leptospirosis. Conventional pathological diagnostic methods do not allow fort issue antigen recognition, thus making the IHC technique important to facilitate conclusive antigen sample verification. In the liver, leptospires were detected mostly between the sinusoids, hepatocytes, and Kupffer cells. In kidney tissues, staining indicated small agglomerates in the tubular lumen, interstitium, and glomeruli. All these forms of presentation have been previously reported. Considering that we detected the highest number of positive samples in lung tissue, followed by those from liver and kidney tissue, we argue that the IHC technique, when applied to samples of these three tissues, decreases the chance of false negatives. Anatomopathological studies of primate leptospirosis are scarce. In dogs, renal lesions are characterized by the necrosis and degeneration of tubular epithelium, cellular debris, and hyaline cylinders. In the liver, hepatocyte cord dissociation and biliary pigment accumulation within the canaliculi and hepatocellular necrosis are observed. These findings are similar to those from our study. In the lung, diffuse alveolar lesions are reported, with hemorrhage and edema, in addition to capillaritis. The high frequency of Leptospira-positive animals determined by serological examination was consistent with the IHC findings, thus confirming the pathogen’s high prevalence in neotropical primate populations in the studied region. Serological surveys on primate populations have already been carried out and have revealed frequency and serovar variations between regions. Immunohistochemical examination allows the detection of leptospires in various tissues and should be used based on the characteristics of the investigated case

    Factors associated with red blood cell transfusions in very-low-birth-weight preterm infants in Brazilian neonatal units

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    Preterm infants in neonatal intensive care units frequently receive red blood cells (RBC) transfusions due to the anemia of prematurity. A number of variables related to gestational age, severity of illness and transfusion practices adopted in the neonatal unit where the neonate was born may contribute to the prescription of RBC transfusions. This study aimed to analyse the frequency and factors associated with RBC transfusions in very-low-birth-weight preterm infants. A prospective cohort of 4283 preterm infants (gestational age: 29.9 ± 2.9 weeks; birth weight: 1084 ± 275 g) carried out at 16 university hospitals in Brazil between January 2009 and December 2011 was analysed. Factors associated with RBC transfusions were evaluated using univariate and multiple logistic regression analysis. A total of 2208 (51.6%) infants received RBC transfusions (variation per neonatal unit: 34.1% to 66.4%). RBC transfusions were significantly associated with gestational age (OR: -1.098; 95%CI: -1.12 to -1.04), SNAPPE II score (1.01; 1.00-1.02), apnea (1.69; 1.34-2.14), pulmonary hemorrhage (2.65; 1.74-4.031), need for oxygen at 28 days of life (1.56; 1.17-2.08), clinical sepsis (3.22; 2.55-4.05), necrotising enterocolitis (3.80; 2.26-6.41), grades III/IV intraventricular hemorrhage (1.64; 1.05-2.58), mechanical ventilation (2.27; 1.74-2.97), use of umbilical catheter (1.86; 1.35-2.57), parenteral nutrition (2.06; 1.27-3.33), >60 days of hospitalization (5.29; 4.02-6.95) and the neonatal unit where the neonate was born. The frequency of RBC transfusions varied among neonatal intensive care units. Even after adjusting for adverse health conditions and therapeutic interventions, the neonatal unit continued to influence transfusion practices in very-low birth-weight infants

    T-piece versus self-inflating bag ventilation in preterm neonates at birth

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    Objective To verify whether the use of the T-piece resuscitator compared with the self-inflating bag in preterm infants ventilated at birth modifies survival to hospital discharge without major morbidities. Design Pragmatic prospective cohort study. Setting 20 Brazilian university hospitals of Brazilian Network on Neonatal Research. Patients were 1962 inborn infants in 2014-2015 ventilated at birth with 23-33' weeks gestation and birth weight 400-1499 g without malformations. Patients transferred until the 27th day after birth were excluded. Interventions Positive pressure ventilation at birth with T-piece resuscitator or self-inflating bag without positive end expiratory pressure valve. Intervention with ventilation followed the Brazilian Society of Pediatrics guidelines. The choice of the equipment was at the neonatologist's discretion in each delivery. The main outcome measures were survival to hospital discharge without bronchopulmonary dysplasia, severe peri-intraventricular haemorrhage and periventricular leucomalada. Logistic regression adjusted for confounding variables was applied for main outcome. Results 1456 (74%) were only ventilated with T-piece resuscitator and 506 (26%) with the self-inflating bag. The characteristics of those ventilated with T-Piece resuscitator versus self-inflating bag were birth weight 969 +/- 277 vs 941 +/- 279 g, gestational age 28.2 +/- 2.5 vs 27.8 +/- 2.7 weeks and survival to hospital discharge without major morbidities 47% vs 35%, Logistic regression adjusted for maternal characteristics, obstetric and neonatal morbidities showed that the T-piece resuscitator increased the chance of survival to hospital discharge without major morbidities (OR=1.38; 95% Cl 1.06 to 1.80; Hosmer-Lemeshow goodness of fit: 0.695). Conclusion This study is the first that highlights the effectiveness of T-piece resuscitator ventilation in improving relevant outcomes in preterm neonates.Univ Fed Sao Paulo, Div Neonatal Med, Sao Paulo, BrazilUniv Sao Paulo, Dept Pediat, Fac Med Ribeirao Preto, Ribeirao Preto, BrazilUniv Fed Maranhao, Dept Pediat, Sao Luis, BrazilUniv Estadual Campinas, Dept Pediat, Fac Ciencias Med, Campinas, SP, BrazilUniv Sao Paulo, Dept Pediat, Fac Med, Sao Paulo, BrazilUniv Estadual Paulista, Div Neonatol, Fac Med Botucatu, Botucatu, SP, BrazilFundacao Oswaldo Cruz, Div Neonatol, Rio De Janeiro, BrazilPontificia Univ Catolica Rio Grande do Sul, Dept Pediat, Hosp Sao Lucas, Fac Med, Porto Alegre, RS, BrazilUniv Fed Rio Grande do Sul, Div Neonatol, Hosp Clin Porto Alegre, Porto Alegre, RS, BrazilUniv Estado Rio de Janeiro, Dept Pediat, Hosp Univ Pedro Ernesto, Rio De Janeiro, BrazilUniv Fed Minas Gerais, Div Neonatol, Belo Horizonte, MG, BrazilUniv Fed Uberlandia, Pediat, Uberlandia, MG, BrazilMaternidade Hilda Brandao, Dept Pediat, Fac Ciencias Med Minas Gerais, Belo Horizonte, MG, BrazilUniv Sao Paulo, Sch Med, Dept Pediat, Sao Paulo, SP, BrazilHosp Estadual Sumare, Neonatal Div, Sumare, BrazilHosp Geral Pirajussara, Neonatal Unit, Taboao Da Serra, BrazilHosp Estadual Diadema, Neonatal Unit, Diadema, BrazilUniv Estadual Londrina, Dept Pediat, Hosp Univ, Curitiba, Parana, BrazilUniv Fed Parana, Dept Pediat, Hosp Clin, Curitiba, Parana, BrazilInst Med Integral Prof Fernando Figueira, Dept Pediat, Recife, PE, BrazilInst Fernandes Figueira FIOCRUZ, Dept Pediat, Rio De Janeiro, BrazilUniv Fed Sao Paulo, Div Neonatal Med, Sao Paulo, BrazilWeb of Scienc

    A 'teoria da práxis': retomando o referencial marxista para o enfrentamento do capitalismo no campo da saúde The 'theory of praxis: ' retrieving the marxist framework to confront capitalism in the health field

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    Este ensaio tem a intenção de recolocar o marxismo - como corpo epistemológico, teórico, metodológico e político voltado para a superação do capitalismo - na pauta do debate conceitual e político do campo da Saúde Coletiva. Discute o campo simbólico como campo de expressão de ideologias que sustentam o capitalismo e que utiliza, dentre outras estratégias, o silenciamento sobre o marxismo, e mesmo sobre o capitalismo, para esvaziar a crítica e o questionamento político. Relaciona estes mecanismos à saúde, identificando alguns campos onde o capitalismo opera nesta área. Apresenta alguns fundamentos filosóficos, teóricos e metodológicos da 'teoria da práxis', destacando a unidade indissolúvel entre teoria crítica e ação transformadora. Por fim, conclama os profissionais, pesquisadores e educadores do campo sanitário a se (re)engajarem na luta contra o capitalismo, retomando a bandeira do socialismo, rumo à conquista do efetivo direito à saúde.<br>This essay is intended to retrieve Marxism - as an epistemological, theoretical, methodological and political body aimed toward overcoming Capitalism - in the political and conceptual debate agenda in field of Collective Health. It discusses the symbolic field as a field for the expression of ideologies that underpin capitalism and which uses, among other strategies,the silencing of Marxism, and even of capitalism, to eliminate criticism and political questioning. It relates these mechanisms to health, identifying some fields in which capitalism operates in this area. It presents a few philosophical, theoretical and methodological foundations of the 'theory of praxis,' highlighting the indissoluble unity between critical theory and transformative action. Finally, it urges health care practitioners, researchers and educators to (re)engage in the struggle against Capitalism, taking up the banner of Socialism aiming to achieve the actual right to health
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