a utilização de sequências de imagens

Este projeto tem como enfoque principal o estudo da escrita de textos narrativos, utilizando sequências de imagens ordenadas e legendadas. Baseia-se no método investigação-ação com inspiração no de investigação sobre a própria prática. Teve-se sempre em conta a importância da planificação para auxiliar a criança na estruturação do seu texto. As sequências de imagens, neste contexto, são conjuntos de quatro ou cinco imagens que são distribuídas desordenadamente. As crianças, numa primeira fase, devem ordená-las e, de seguida, legendá-las, de acordo com o observado na figura. Após a legendagem, preenchem os vários campos de uma planificação, adaptada (Niza, Segura & Mota, 2011) para, posteriormente, escreverem o seu texto. Para analisar e avaliar as produções, utilizei as técnicas de análise estatística e a análise de conteúdo. Para a primeira, foram construídos gráficos a partir de dados obtidos por mim para ser possível que fosse lida eficaz e rapidamente, enquanto que para a segunda tive em conta o que foi escrito, nas sequências, nas planificações e nos textos, sendo que verifiquei se as produções continham as várias categorias da narrativa, a saber, as personagens, o espaço, o tempo e a ação.This project has, as the main focus, the writing of narrative texts, using sorted and labelled sequences of images and a text planning to help students to structure their narrative texts. It is based on the Action-Research method, with inspiration in the researching practice. The sequences of images are sets of four or five images which are distributed disorderly and, in a first phase, students must sort and then label them according to what they observed in the image. After that, students must fill in the several fields of a text plan, adapted from Niza, Segura & Mota, 2011, and subsequently write the narrative text. In order to analyse and evaluate the students’ productions, I used statistics analysis and content analyses. For the first, graphics were made with data which were gathered by me, so that it would be possible to read it effectively and rapidly, while for the second, I had in account what was written by the students in the sequences, plans and texts. I checked if the students’ productions contained the several categories of the narrative such as characters, space, time and action.Escola Superior de Educação, Instituto Politécnico de Setúba

Caraterização dos canais de comercialização do vinho no Alentejo: uma abordagem com base na teoria dos custos de transação

O setor do vinho tanto em Portugal como no Alentejo tem vindo a afirmar-se ao longo dos anos com produtos genuinamente únicos que têm ganho reputação no mercado doméstico e no mercado externo. O potencial de diferenciação e a reduzida dimensão do mercado nacional para absorver adequadamente a produção, leva à necessidade de uma estratégia de consolidação no mercado externo. Desta forma, é importante uma gestão eficiente dos canais de comercialização. Deste modo, este estudo tem como objetivo caraterizar a estrutura dos canais de comercialização das empresas do setor do vinho no Alentejo e avaliar o seu nível de integração. Para além de compreender o nível de integração dos canais de comercialização dos vinhos e as principais razões que o determinam, definiram-se quatro objetivos específicos: a) Identificar os canais de comercialização utilizados pelas empresas e a forma como os agentes se relacionam neles; b) Avaliar a influência que o grau de incerteza tem no nível de integração dos canais de comercialização; c) Avaliar a influência que a especificidade dos ativos tem o nível de integração dos canais de comercialização e; d) Avaliar como é que a frequência das transações afeta o grau de integração dos canais de comercialização. A metodologia utilizada seguiu uma abordagem mista, baseada na análise qualitativa e na análise quantitativa. A primeira teve em conta a realização de entrevistas (reuniões) com especialistas do setor e a última a elaboração de um questionário para aplicar a uma amostra de empresários do setor do vinho no Alentejo. Os resultados obtidos parecem indicar, como era esperado, que as empresas não utilizam unicamente um canal de comercialização de exportação. E ao contrário do que é referido na literatura, as empresas objeto de estudo geralmente não coordenam as suas transações através de contratos. No que respeita ao poder exercido no canal, este poderá variar em função do tipo de canal e do tipo de contrato. Os resultados permitiram ainda concluir que a incerteza afeta os custos de transação, bem como os ativos específicos, nomeadamente associados aos recursos humanos. Verificou-se também que quanto maior for a frequência das transações, maior é o nível de integração dos canais de comercialização; Abstract: In Portugal as in Alentejo the wine sector has been affirmed with genuine and unique products, which have gained a reputation in both the household market and foreign market. The potential for differentiation and a small size of the household market to absorb production lead to a consolidation strategy in the foreign markets. Thus, an efficient management of marketing channels is important. Therefore, this study aims to characterise the marketing channels structure of the wine sector in Alentejo, and assess its integration level. In addition, to understand the marketing channels protection level of the wines in Alentejo and the main raisons that determine them, four specific objectives were defined: a) identifying the marketing channels that are used by wine companies and how the channels’ agents are related among them to make the transactions; b) assessing the influence of uncertainty on the marketing channels integration; c) assessing the influence of asset specificity on the marketing channels integration; and d) assessing how transaction frequency affects the marketing channels integration. The methodology followed a mixed approach based on the qualitative and quantitative analyses. The former took into account interviews (meetings) with experts from the wine sector, and the latter comprised a survey to be applied in a sample of wine producers from Alentejo. As expected, the results indicate that wine companies do not use only one kind of channel to export. Contrary to the literature, the studied wine companies generally do not make the coordination of their transactions by using contracts. With regards the marketing channel power, it may vary with the type of channel or contract used. The results still allowed conclude that uncertainty affects the transaction costs, as well as the specific assets, namely the human resources. One also verified that as greater is the transaction frequency higher is the marketing channels integration

Coherent pairs of linear functionals on the unit circle

In this paper we extend the concept of coherent pairs of measures from the real line to Jordan arcs and curves. [...]info:eu-repo/semantics/publishedVersio

Selective extraction and stabilization of bioactive compounds from rosemary leaves using a biphasic NADES

Publisher Copyright: Copyright © 2022 Vieira, Rebocho, Craveiro, Paiva and Duarte.Rosemary (Rosmarinus officinalis) is a natural source of bioactive compounds that have high antioxidant activity. It has been in use as a medicinal herb since ancient times, and it currently is in widespread use due to its inherent pharmacological and therapeutic potential, in the pharmaceutical, food, and cosmetic industries. Natural deep eutectic systems (NADESs) have recently been considered as suitable extraction solvents for bioactive compounds, with high solvent power, low toxicity, biodegradability, and low environmental impact. The present work concerns the extraction of compounds such as rosmarinic acid, carnosol, carnosic acid, and caffeic acid, from rosemary using NADESs. This extraction was carried out using heat and stirring (HS) and ultrasound-assisted extraction (UAE). A NADES composed of menthol and lauric acid at a molar ratio of 2:1 (Me:Lau) extracted carnosic acid and carnosol preferentially, showing that this NADES exhibits selectivity for nonpolar compounds. On the other hand, a system of lactic acid and glucose (LA:Glu (5:1)) extracted preferentially rosmaniric acid, which is a more polar compound. Taking advantage of the different polarities of these NADESs, a simultaneous extraction was carried out, where the two NADESs form a biphasic system. The system LA:Glu (5:1)/Men:Lau (2:1) presented the most promising results, reaching 1.00 ± 0.12 mg of rosmarinic acid/g rosemary and 0.26 ± 0.04 mg caffeic acid/g rosemary in the more polar phase and 2.30 ± 0.18 mg of carnosol/g of rosemary and 17.54 ± 1.88 mg carnosic acid/g rosemary in the nonpolar phase. This work reveals that is possible to use two different systems at the same time and extract different compounds in a single-step process under the same conditions. NADESs are also reported to stabilize bioactive compounds, due to their interactions established with NADES components. To determine the stability of the extracts over time, the compounds of interest were quantified by HPLC at different time points. This allows the conclusion that bioactive compounds from rosemary were stable in NADESs for long periods of time; in particular, carnosic acid presented a decrease of only 25% in its antioxidant activity after 3 months, whereas the carnosic acid extracted and kept in the methanol was no longer detected after 15 days. The stabilizing ability of NADESs to extract phenolic/bioactive compounds shows a great promise for future industrial applications.publishersversionpublishe

Experimental Determination and Modeling of the Phase Behavior of the CO2 + Propionic Anhydride Binary System at High Pressure

The phase equilibrium of the binary system (CO2 + propionic anhydride) was determined experimentally at temperatures of 308, 313, and 323 K and pressures up to 10 MPa. Measurements were carried out in a high-pressure visual cell with variable volume. The experimental data were modeled using the Peng–Robinson equation of state and the Mathias–Klotz–Prausnitz mixing rule. A good correlation was achieved with this model, with a total average absolute deviation of 0.21%

A Rare Cause of Abdominal Pain: Intestinal Angioedema

Angioedema with evidence of mucosal swelling is a rare condition that can be caused by angiotensin-converting enzyme inhibitors. The authors describe a case of a 28-year-old woman medicated with lisinopril 2 months before, with abdominal pain associated with nausea, vomiting and a onetime watery stool. A small volume of ascites was found in the abdominal ultrasound and the parietal thickening of the middle ileum in the abdominal computed tomography (CT). The complementary study was negative. The symptoms stopped after drug withdrawal. The authors intend to warn to this diagnosis, which has non-specific symptoms and if not suspected, can lead to a great morbidity for patients. The authors also intend to identify various signs that can be clues for its correct diagnosis.info:eu-repo/semantics/publishedVersio

Evaluation of the volatiles’ chemical profile and antibacterial activity of Lavandula stoechas L. extracts obtained by supercritical carbon dioxide

Funding Information: This project has received funding from the European Union's Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement No 101003396; European Union's Horizon 2020 (European Research Council) under grant agreement No ERC-2016-CoG 725034; National funds (FCT/MCTES, Fundação para a Ciência e Tecnologia and Ministério da Ciência, Tecnologia e Ensino Superior) through the projects UIDB/50006/2020 and UIDP/50006/2020. The biological activity has been supported by research ID: 3105-12-21. The authors would like to thank Ana Jovanoski for her editorial support. Funding Information: This project has received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement No 101003396 ; European Union’s Horizon 2020 ( European Research Council ) under grant agreement No ERC- 2016-CoG 725034 ; National funds (FCT/MCTES, Fundação para a Ciência e Tecnologia and Ministério da Ciência, Tecnologia e Ensino Superior) through the projects UIDB/50006/2020 and UIDP/50006/2020 . The biological activity has been supported by research ID: 3105-12-21. The authors would like to thank Ana Jovanoski for her editorial support. Publisher Copyright: © 2023 The AuthorsThe goal of this study was to establish a green methodology for obtaining safe, high-quality, and potent antibacterial extracts of Lavandula stoechas flowers. Supercritical carbon dioxide (scCO2) at different conditions (pressure 100–300 bar, temperature 40 and 60 °C, and CO2 flow 10–30) was applied. Moreover, the impact of the parameters on the extraction yield, chemical profile of the extracts, and the activity against Gram-positive and Gram-negative bacteria was investigated. ScCO2 extraction kinetics was investigated by modelling the extraction curves using the models described by Brunner, Martinez, and their modifications. In vitro assays were applied to estimate the susceptibility of Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, and Staphylococcus aureus to extracts. A pattern recognition approach was applied to explore the correlations between the employed scCO2 parameters, detected volatile compounds, and extracts’ antibacterial properties. The achieved yield ranged from 1.17 to 2.4% (w/w), with oxygenated monoterpenes fenchone (5.76–22.72%) and verbenone (8.04.-18.65%) as the most abundant ones. The most significant susceptibility of Gram-positive and Gram-negative bacteria was attributed to extracts obtained at 200 bar, with minimal inhibitory concentration in the range 2.71 ± 1.31–20.69 ± 0.91 mgmL−1 and 3.39 ± 0.48–34.28 ± 5.94 mgmL−1, respectively. The obtained extracts represent safe, viable, and promising alternatives for tackling antimicrobial resistance. Moreover, by varying process conditions, it is possible to adjust the chemical profile and the activity of the extracts according to the target purpose. Furthermore, the requirements of clean and sustainable technologies, such as environmental preservation, rational use of renewable resources, and provision of natural, safe, and high-quality extracts can be met.publishersversionpublishe

Fractionated extraction of polyphenols from mate tea leaves using a combination of hydrophobic/ hydrophilic NADES

Publisher Copyright: © 2022 The AuthorsA new methodology for the selective extraction of antioxidants from mate tea leaves (and decaffeinated mate tea leaves), using different natural deep eutectic systems (NADES), is reported in this paper. A fractionated extraction was carried out and the optimization of the extraction conditions such as solid/liquid ratio, temperature, time, stirring and the use of ultrasound assisted extraction (UAE) technology was performed. The results demonstrate that a sequential extraction using, in a first step, an hydrophobic system Men:Lau (2:1) and, in a second step, an hydrophilic lactic acid-based NADES, leads to two distinct extracts: the first one rich in pigments and the second one rich in polyphenols. NADES systems were able to extract 30% more of the polyphenolic components of the mate tea leaves matrices, when compared with traditional solvents/techniques. Moreover, it has been shown that the incorporation of the extract in the NADES, compared to the same extract in aqueous medium was beneficial for the stabilization of the antioxidants. It maintains their functionality at least for three months, reaching 41% more versus the extracts obtained by traditional solvents/techniques. The absence of caffeine in the extracts did not shown to have any effects on the stability results.publishersversionpublishe

Characterization of the Thermostable Biosurfactant Produced by Burkholderia thailandensis DSM 13276

Funding Information: Technology: 31961133015; Shandong University: 31961133014). C.V.G., A.E. and A.T.R. acknowledge FCT I.P. for the Ph.D. grants 2021.05798.BD, 2021.05014.BD and 2020.06470.BD, respectively. Funding Information: ThTeh ethgelrymcoalli pstoapbeilpittyid oef bthioesuBr. ftahcatialanntdseencsriestbediobsuyrBfaucrtkahnotl dweraisa ethvaailluanatdeedn sbisyD exSpMos1i3n2g7 6 thwe absiodpeomlyomnsetrr aitne danto apquosesoeusss svoalluutaiobnle tsou raf atecme-apcetriavteuprer oopfe 1r2ti1e s°,Cnainm aenly ,aautloowclaCvMe (C0.9a8nd bar) for 20 min. Interestingly, the treated solution maintained its shear-thinning behavior, with a slight increase in the flow behavior index value (0.46) compared to the untre◦ ated biosurfactant solution (0.44) (refer to Figure S4 for supporting information on fitting the power law model). The thermally treated biosurfactant also maintained a surface tension value of 40.36 ± 0.5 mN/m, which was identical to that of the untreated solution (40.31 ± 0.agent26 mNin/mfood), thus conand/orfirmcosmeticing its prthermoducts/pral stabocessing,ility. and for bioremediation. The emulsifying ability of the treated biosurfactant, on the other hand, was negatively affected, with a reduction in the EA to 50.5 ± 0.9%, which is around half of the value observed for the non-treated biosurfactant (100.0 ± 0.0%). Different biosurfactants (e.g., glAyucothliopridCso)n atrlisbousthioonws:eCdoan scleipgthuta dliezcartieoans,eC i.nV.tGh.e, eF.mF.ualnsdifyCi.nAg.Vc.aTp.;amciettyhoadftoelroghye,aCt .tVre.Ga.t,mAe.Tn.tR ., at similar temperatures [64]. Nevertheless, the value is still within the range reported for good EA (≥50%) [59]. Moreover, Triton X-100 also suffered a similar reduction in its emulsification ability, as the EA of the emulsions stabilized with the autoclaved compound also reduced from 43.9 ± 2.1% to 33.4 ± 5.2%. These results underline the potential of the B. thIa.Pil.a, nindetnhseisscboipoesuorfftahcetapnrot jfeocrts uUseID, fPo/r0e4x37a8m/p20le20, ianntdheU fIDooBd/ 0i4n3d7u8/st2r0y2,0 inofwthheicRhestheaercthemUpneitr-on atAuprepslieadreMeolleevcuatlaerdBdiouscriienngcepsr—oUceCsIsBiInOgaonrd tthhee fpirnoajelcpt rLoAd/uPc/t0 i1s40c/o2n0s2u0mofetdh.e Associate Laboratory Institute for Health and Bioeconomy—i4HB, and by the European Union’s Horizon 2020 research and 4. Coninnovcalustionioprnos gram through Project Bio Innovation of a Circular Economy for Plastics (BioICEP), under The glycolipopeptide biosurfactant secreted by Burkholderia thailandensis DSM 13276 numbers: Institute of Microbiology, Chinese Academy of Sciences: 31961133016; Beijing Institute of was demonstrated to possess valuable surface-active properties, namely, a low CMC and high EA for almond and sunflower oils, and for benzene. Moreover, the biosurfactant showed good thermostability, with a thermal degradation temperature above 200 °C, and the ability to maintain stable rheological and surface-active properties, as well as good EA Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.Biosurfactants synthesized by microorganisms represent safe and sustainable alternatives to the use of synthetic surfactants, due to their lower toxicity, better biodegradability and biocompatibility, and their production from low-cost feedstocks. In line with this, the present study describes the physical, chemical, and functional characterization of the biopolymer secreted by the bacterium Burkholderia thailandensis DSM 13276, envisaging its validation as a biosurfactant. The biopolymer was found to be a glycolipopeptide with carbohydrate and protein contents of 33.1 ± 6.4% and 23.0 ± 3.2%, respectively. Galactose, glucose, rhamnose, mannose, and glucuronic acid were detected in the carbohydrate moiety at a relative molar ratio of 4:3:2:2:1. It is a high-molecular-weight biopolymer (1.0×107Da) with low polydispersity (1.66), and forms aqueous solutions with shear-thinning behavior, which remained after autoclaving. The biopolymer has demonstrated a good emulsionstabilizing capacity towards different hydrophobic compounds, namely, benzene, almond oil, and sunflower oil. The emulsions prepared with the biosurfactant, as well as with its autoclaved solution, displayed high emulsification activity (>90% and ~50%, respectively). Moreover, the almond and sunflower oil emulsions stabilized with the biosurfactant were stable for up to 4 weeks, which further supports the potential of this novel biopolymer for utilization as a natural bioemulsifier.publishersversionpublishe

Preparation and characterization of films based on a natural P(3HB)/mcl-PHA blend obtained through the co-culture of Cupriavidus necator and Pseudomonas citronellolis in apple pulp waste

The co-culture of Cupriavidus necator DSM 428 and Pseudomonas citronellolis NRRL B-2504 was performed using apple pulp waste from the fruit processing industry as the sole carbon source to produce poly(3-hydroxybutyrate), P(3HB) and medium-chain length PHA, mcl-PHA, respectively. The polymers accumulated by both strains were extracted from the co-culture’s biomass, resulting in a natural blend that was composed of around 48 wt% P(3HB) and 52 wt% mcl-PHA, with an average molecular weight of 4.3 105 Da and a polydispersity index of 2.2. Two melting temperatures (Tm) were observed for the blend, 52 and 174 C, which correspond to the Tm of the mcl-PHA and P(3HB), respectively. P(3HB)/mcl-PHA blend films prepared by the solvent evaporation method had permeabilities to oxygen and carbon dioxide of 2.6 and 32 Barrer, respectively. The films were flexible and easily deformed, as demonstrated by their tensile strength at break of 1.47 0.07 MPa, with a deformation of 338 19% until breaking, associated with a Young modulus of 5.42 1.02 MPa. This study demonstrates for the first time the feasibility of using the co-culture of C. necator and P. citronellolis strains to obtain a natural blend of P(3HB)/mcl-PHA that can be processed into films suitable for applications ranging from commodity packaging products to high-value biomaterialsinfo:eu-repo/semantics/publishedVersio