302 research outputs found
Direct mass measurements beyond the proton drip-line
First on-line mass measurements were performed at the SHIPTRAP Penning trap
mass spectrometer. The masses of 18 neutron-deficient isotopes in the
terbium-to-thulium region produced in fusion-evaporation reactions were
determined with relative uncertainties of about , nine of them
for the first time. Four nuclides (Ho and Tm) were
found to be proton-unbound. The implication of the results on the location of
the proton drip-line is discussed by analyzing the one-proton separation
energies
Transfection and homologous recombination involving single-stranded DNA substrates in mammalian cells and nuclear extracts.
Position-sensitive ion detection in precision Penning trap mass spectrometry
A commercial, position-sensitive ion detector was used for the first time for
the time-of-flight ion-cyclotron resonance detection technique in Penning trap
mass spectrometry. In this work, the characteristics of the detector and its
implementation in a Penning trap mass spectrometer will be presented. In
addition, simulations and experimental studies concerning the observation of
ions ejected from a Penning trap are described. This will allow for a precise
monitoring of the state of ion motion in the trap.Comment: 20 pages, 13 figure
New Method to Prepare Mitomycin C Loaded PLA-Nanoparticles with High Drug Entrapment Efficiency
The classical utilized double emulsion solvent diffusion technique for encapsulating water soluble Mitomycin C (MMC) in PLA nanoparticles suffers from low encapsulation efficiency because of the drug rapid partitioning to the external aqueous phase. In this paper, MMC loaded PLA nanoparticles were prepared by a new single emulsion solvent evaporation method, in which soybean phosphatidylcholine (SPC) was employed to improve the liposolubility of MMC by formation of MMCâSPC complex. Four main influential factors based on the results of a single-factor test, namely, PLA molecular weight, ratio of PLA to SPC (wt/wt) and MMC to SPC (wt/wt), volume ratio of oil phase to water phase, were evaluated using an orthogonal design with respect to drug entrapment efficiency. The drug release study was performed in pH 7.2 PBS at 37 °C with drug analysis using UV/vis spectrometer at 365 nm. MMCâPLA particles prepared by classical method were used as comparison. The formulated MMCâSPCâPLA nanoparticles under optimized condition are found to be relatively uniform in size (594 nm) with up to 94.8% of drug entrapment efficiency compared to 6.44 ÎŒm of PLAâMMC microparticles with 34.5% of drug entrapment efficiency. The release of MMC shows biphasic with an initial burst effect, followed by a cumulated drug release over 30 days is 50.17% for PLAâMMCâSPC nanoparticles, and 74.1% for PLAâMMC particles. The IR analysis of MMCâSPC complex shows that their high liposolubility may be attributed to some weak physical interaction between MMC and SPC during the formation of the complex. It is concluded that the new method is advantageous in terms of smaller size, lower size distribution, higher encapsulation yield, and longer sustained drug release in comparison to classical method
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