Sediments from Lago di Mezzano, central Italy: a record of Lateglacial/Holocene climatic variations and anthropogenic impact

Microscopic, geochemical and pollen analysis of sediment samples of a Lateglacial/Horocene profile from Lago di Mezzano, a maar lake in central Italy, reveals evidence of significant climatic and human-induced environmental changes. Time control is provided by a combination of varve chronology and radiocarbon dating. The well-known Lateglacial climatic variations, a warmer interstadial and the Younger Dryas cold phase are clearly represented in all the parameters. During the interval between 9200 and 5600 cal. BP of the Holocene climatic optimum, annually laminated, organic-rich diatom gyttja was deposited. Two periods of diminished total organic carbon are identified within this interval. The first one (P1) ranges from 8200 to 7800 cal. BP while the second (P2) is centred around 6500 cal. BP. During P1, a predominance of diatoms (Stephanodiscus parvus and S. minutulus) over other algae (represented by the total organic carbon content) is observed. The timing of this period coincides with the early- to mid-Holocene climatic transition, reported from ice cores and lake sediments (Stager and Mayewski, 1997). P2 is characterized by a decrease in all biogenic parameters including biogenic opal, organic carbon as well as arboreal pollen. From 5000 cal. BP to date, the sediment pattern changes coincide with the mid-Holocene climatic deterioration. In addition to these natural variations, human impact has been recorded and recognized from sedimentological features such as turbidites and charcoal, as well as from reduced arboreal pollen content. Two Middle Bronze Age (3700 cal. BP and 3300 cal. BP), Etruscan/early Roman (2500 cal. BP), Longobardic (AD 900) and 'modern settlements' (since no 1700) have been distinguished on the basis of these data

Regulation of the mammalian elongation cycle by subunit rolling: a eukaryotic-specific ribosome rearrangement

The extent to which bacterial ribosomes and the significantly larger eukaryotic ribosomes share the same mechanisms of ribosomal elongation is unknown. Here, we present subnanometer resolution cryoelectron microscopy maps of the mammalian 80S ribosome in the posttranslocational state and in complex with the eukaryotic eEF1AVal-tRNAGMPPNP ternary complex, revealing significant differences in the elongation mechanism between bacteria and mammals. Surprisingly, and in contrast to bacterial ribosomes, a rotation of the small subunit around its long axis and orthogonal to the well-known intersubunit rotation distinguishes the posttranslocational state from the classical pretranslocational state ribosome. We term this motion "subunit rolling." Correspondingly, a mammalian decoding complex visualized in substates before and after codon recognition reveals structural distinctions from the bacterial system. These findings suggest how codon recognition leads to GTPase activation in the mammalian system and demonstrate that in mammalia subunit rolling occurs during tRNA selection

Structures of ribosome-bound initiation factor 2 reveal mechanism of subunit association

Throughout the four phases of protein biosynthesis—initiation, elongation, termination, and recycling—the ribosome is controlled and regulated by at least one specified translational guanosine triphosphatase (trGTPase). Although the structural basis for trGTPase interaction with the ribosome has been solved for the last three steps of translation, the high-resolution structure for the key initiation trGTPase, initiation factor 2 (IF2), complexed with the ribosome, remains elusive. We determine the structure of IF2 complexed with a nonhydrolyzable guanosine triphosphate analog and initiator fMet-tRNAiMet in the context of the Escherichia coli ribosome to 3.7-Å resolution using cryo-electron microscopy. The structural analysis reveals previously unseen intrinsic conformational modes of the 70S initiation complex, establishing the mutual interplay of IF2 and initator transfer RNA (tRNA) with the ribsosome and providing the structural foundation for a mechanistic understanding of the final steps of translation initiation

Response of littoral chironomid community and organic matter to late glacial lake level and environmental changes at Lago dell'Accesa (Tuscany, Italy).

International audienceThis study focuses on the response of lacustrine littoral chironomid communities to late glacial changes in limnological, environmental and climate conditions in the Mediterranean context. Late glacial chironomid (Diptera: Chironomidae) assemblages, organic petrography and geochemistry were analysed in a sediment core from the littoral zone of Lago dell'Accesa (Tuscany, Italy), where the lake-level fluctuations and the vegetation history have been previously reconstructed. Comparison of the chironomid stratigraphy to other proxies (pollen assemblages, organic petrography and geochemistry, lake-level) and regional climate reconstruction suggested the predominant influence of lake-level changes on the littoral chironomid fauna. The main lowering events that occurred during the Oldest and the Younger Dryas were followed by higher proportions of taxa typical of littoral habitats. A complementary study of organic matter suggested the indirect impact of lake-level on the chironomids through changes in humic status and habitat characteristics, such as the type of substrate and aquatic macrophyte development. Several chironomid taxa, such as Glyptotendipes, Microtendipes and Cricotopus type patens, were identified as possible indicators of low lake-level in the late glacial records. Nevertheless, this study suggested that parallel analyses of organic matter and chironomid assemblages may be needed to circumvent misinterpretation of littoral chironomid assemblage stratigraphy. There was a weak response of the chironomid assemblages to small lake-level lowerings that corresponded to the Older Dryas and Preboreal oscillations. A higher level of determination, e.g. to the species group level, may be necessary to increase the sensibility of the indicators to lake-level changes