Branded content and luxury fashion: analysis of audio-visual content on YouTube (2008-2019)

Las redes sociales y la democratización de la moda han impactado en las estrategias comunicativas del sector del lujo. Este artículo realiza un análisis de descriptivo a través del análisis de contenido de todos los vídeos alojados – 4079 – en los canales de Youtube de las 12 principales marcas de moda de lujo. El principal objetivo es conocer la evolución de los contenidos audiovisuales de las principales marcas de moda de lujo en Youtube e identificar los principales formatos. Los resultados aportan una clasificación de los principales formatos utilizados por el sector del lujo, así como su capacidad de interacción. La investigación concluye que las marcas de moda de lujo usan más formatos convencionales que estrategias de contenido asociadas al branded content. Del mismo modo, se aprecia que las marcas de moda de lujo apenas interaccionan con su público objetivo.Social networks and the democratisation of fashion have had an impact on the communication strategies deployed by the luxury sector. This article performs a descriptive analysis by way of a content analysis of all the videos hosted – 4079 – on the Youtube channels of the 12 main luxury faschion brands. The aim is to comprehend the evolution of the audio-visual content of the main luxury fashion brands on Youtube and to identify the main formats. The results provide a classification of the main formats used by the luxury sector, as well as their capacity for interaction. The research concludes that luxury fashion brands deploy more conventional formats than content strategies associated with branded content. . Similarly, it can be seen that luxury fashion brands interact with their target audience only marginally

Branded content and luxury fashion: analysis of audio-visual content on YouTube (2008-2019)

Las redes sociales y la democratización de la moda han impactado en las estrategias comunicativas del sector del lujo. Este artículo realiza un análisis descriptivo a través del análisis de contenido de todos los vídeos alojados —4079— en los canales de YouTube de las 12 principales marcas de moda de lujo. El principal objetivo es conocer la evolución de los contenidos audiovisuales de las principales marcas de moda de lujo en YouTube e identificar los principales formatos. Los resultados aportan una clasificación de los principales formatos utilizados por el sector del lujo, así como su capacidad de interacción. La investigación concluye que las marcas de moda de lujo usan más formatos convencionales que estrategias de contenido asociadas al branded content. Del mismo modo, se aprecia que las marcas de moda de lujo apenas interaccionan con su público objetivo.Social networks and the democratisation of fashion have had an impact on the communication strategies deployed by the luxury sector. This article performs a descriptive analysis by way of a content analysis of all the videos hosted —4079— on the YouTube channels of the 12 main luxury fashion brands. The aim is to comprehend the evolution of the audio-visual content of the main luxury fashion brands on YouTube and to identify the main formats. The results provide a classification of the main formats used by the luxury sector, as well as their capacity for interaction. The research concludes that luxury fashion brands deploy more conventional formats than content strategies associated with branded content. Similarly, it can be seen that luxury fashion brands interact with their target audience only marginally

Methodologies for teaching an engineering subject in different countries: comparison and results

Engineering or technical degrees are difficult to teach and, consequently, have always been characterized by a large number of academic failures. Therefore, continuous assessment has been applied to classes of similar content, related to Port and Coastal Engineering during these last years in three different Universities worldwide: University of La Republica (Montevideo, Uruguay), Nova de Lisboa (Portugal) and Cadiz (Spain). This paper presents different methodologies used to teach and evaluate these courses at each University, together with the results of the evaluations of the students who were enrolled during the current and previous stages. Generally, a decrease in the number of students who abandon the classes has been noticed together with an increase in the percentage of students who pass and an improvement of their grades, except at the University Nova de Lisboa were the results have remained stable. In addition, changes experienced in the courses are discussed herein by comparing the percentage of success in the different locations. Moreover, influence of the different methodologies and the possible reasons for these changes are also presented and analysed. As a conclusion, the improvement in educational outcomes has been achieved through the concurrence of different factors: the existence of more frequent written and/or oral exams, practical examples of case studies as well as access to specific tools of new technology and to documentation specifically prepared for the classes and available online. Evidently, the above mentioned tasks require a strong commitment and great effort by the teaching staff. If human resources diminish, as it is happening in Spain and Portugal due to the budget reduction in education, two difficult questions arise: For how long will teachers’ current effort be maintained? What impact will have their complete devotion to teaching in their research performance

A novel loop-mediated isothermal amplification-based test for detecting Neospora caninum DNA

Background: Neospora caninum is a cyst-forming, coccidian parasite which is known to cause neurological disorders in dogs and abortion and neonatal mortality in cows and other livestock. This study reports the development of a loop-mediated isothermal amplification (LAMP) assay based on the Neospora caninum Nc-5 gene and compares its efficacy for detecting DNA to that of a semi-nested PCR test. Results: Six primers were designed based on the Nc-5 repeat region of N. caninum. Specific LAMP primers led to successful amplification of N. caninum DNA at 63 °C in 30 min. The LAMP assay was highly specific (i.e. it did not reveal cross-reactivity with other parasite species) and had a low N. caninum plasmid DNA limit of detection (1 fg), which is ten times higher than that for the semi-nested PCR. LAMP applicability was evaluated using a set of naturally-infected samples (59 from canine faeces and five from bovine abortions). Thirty-nine percent (25/64) of the naturally-infected samples were positive for N. caninum DNA by LAMP and 36% (23/64) by semi-nested PCR. However, the LAMP assay is much faster to perform than semi-nested PCR and provides results in 30 min. Conclusion: The optimized reaction conditions described in this study resulted in a sensitive, specific and rapid technique for detecting N. caninum DNA. Considering the advantages of LAMP for detecting N. caninum DNA, further assays aimed at testing its usefulness on a wider range of field samples are recommended. © 2017 The Author(s)

Clima y desempeño laboral en los trabajadores en la oficina de logística del gobierno regional de Amazonas 2018

El trabajo de investigación titulada “Clima y Desempeño laboral en el personal en la Oficina de Logística del Gobierno Regional Amazonas 2018”, permitió determinar la relación que existe entre las variables clima laboral y desempeño laboral, se utilizó para tal fin, como instrumento que el cuestionario de tipo Likert que constaba de 50 ítems para clima laboral y una encuesta compuesta por de 38 ítems para la variable desempeño laboral, los cuales fueron aplicados a 46 trabajadores de la oficina de logística del Gobierno Regional Amazonas; los resultados midieron las dimensiones: autorrealización, involucramiento laboral, supervisión, comunicación y condiciones laborales para la variable clima laboral y para el caso de desempeño las dimensiones: satisfacción, comunicación, conocimiento del cargo, valores, desempeño y capacitación. Consecutivamente de la aplicación de los instrumentos y teniendo recogidos los datos se realizó el procesamiento de datos a través del software SPSS Statistics en su versión 24, en la que se estableció la relación del clima laboral y el desempeño laboral en el personal de la Oficina de Logística del Gobierno Regional Amazonas, llegando a la conclusión que los colaboradores se desempeñan mejor, cuando perciben un ambiente laboral agradable y motivador. El método empleado en la investigación tuvo como diseño no experimental cualitativo, de tipo Descriptivo-correlacional causal

Effect of foliar applications of boron on the phenological development and fruit set of purple passion fruits (Passiflora edulis f. edulis Sims)

1 recurso en línea (páginas 20-30).La gulupa es un frutal nativo producido en Colombia y exportado a diferentes mercados internacionales. Los principales problemas que afectan la producción del cultivo están relacionados con la floración y fructificación, debido a limitaciones nutricionales, como la deficiencia de boro (B). La presente investigación tuvo como objetivo, evaluar el efecto de la aplicación foliar de B en el desarrollo fenológico y cuajado del fruto en plantas de gulupa bajo condiciones de campo. El estudio se llevó a cabo en una plantación experimental del municipio de Pasca (Cundinamarca, Colombia), ubicado a 2.006 msnm. Los tratamientos correspondieron a la aplicación foliar de tres dosis de ácido bórico (0; 0,3; 0,6 y 0,9 kg ha-1) aplicados en la etapa de botón floral y cuajado del fruto, durante dos ciclos productivos. Se evaluaron la longitud de las ramas productivas, el número de entrenudos, el porcentaje de cuajado del fruto, el diámetro del fruto y la emisión de botones florales y frutos. La aplicación foliar de B en las dosis evaluadas aumentó la longitud de ramas secundarias, la emisión de botones florales y frutos, y el porcentaje de cuajado del fruto. Los mejores resultados de crecimiento en el primer y segundo ciclo se lograron con 0,3 kg ha-1; aunque el porcentaje de cuajado del fruto del tratamiento 0,6 kg ha-1 fue óptimo para el primer ciclo de producción (85,19%) seguido por 0,3 kg ha-1 para el segundo ciclo de producción (90%). Los frutos del tratamiento 0,3 kg ha-1 desarrollaron un mayor diámetro ecuatorial desde los 21 días después de transplante (ddt), alcanzando el máximo diámetro (55,68 mm) a los 136 ddt.Gulupa or purple passion fruit is a native fruit produced in Colombia and exported to different international markets. Some problems affecting crop production are related to flowering and fruiting, mainly because of nutrient limitations, such as boron (B) deficiency. The objective of this research was to evaluate the effect of foliar boron spraying on the phenological development and fruit set of gulupa plants grown under field conditions. This study was carried out in an experimental plantation in the municipality of Pasca Cundinamarca, Colombia), located at 2,006 m a.s.l. The treatments consisted of three doses of boric acid: T50, T100, T150, and a control, T0, with foliar spray applications in the floral bud and fruit set stages, during two consecutive productive cycles. The length of the productive branches, number of internodes, percentage of fruit set, fruit diameter, and emission of flower buds and fruits were evaluated. The foliar applications of B at the evaluated rates increased the length of the secondary branches, emission of flower buds and fruits, and percentage of fruit set. The best growth results in the first and second cycles were achieved with the T50 treatment although, in terms of fruit set percentage, the T100 treatment was optimal for the first production cycle (85.19%), followed by the T50 treatment for the second production cycle (90%). The fruits of the treatment 0.3 kg ha-1 developed a larger equatorial diameter from 21 days after planting (dap), reaching the maximum diameter (55.68 mm) at 136 dap.Bibliografía: páginas 28-3

Recursos para la Enseñanza de la Alimentación en la Formación Inicial de Maestros (PIMCD, nº 103, convocatoria 2015)

Actualización y mejora de recursos sobre la enseñanza-aprendizaje de la Alimentación para la Formación Inicial de Maestros puestos en practica en el desarrollo docente

Social network analysis as a tool to inform sustainable multi-sectoral management in complex marine socioecosystems

In complex marine socioecosystems, where multiple sectoral activities coexist, management can be especially complicated. This is the case of the coupled Guadalquivir estuary - Gulf of Cadiz (Ge-GoC) system. The Guadalquivir estuary (SW Spain) plays a central role in the whole Gulf of Cadiz since many fish species use these waters as a nursery area. Therefore, not only fisheries management is important but also the environmental status of the estuarine waters. The latter will have an effect on the adult populations via recruitment. Human activities that directly or indirectly influence the estuary include fishing, agriculture, shipping, saltworks, tourism or aquaculture among others. Conflict among them are common. In this context, we have used social network analyses as a first step to create an analysis and decision-support framework to achieve balanced socio-ecological ecosystem based management (EBM) to the Ge-GOC

Comparative study of human embryonic stem cells (hESC) and human induced pluripotent stem cells (hiPSC) as a treatment for retinal dystrophies

Retinal dystrophies (RD) are major causes of familial blindness and are characterized by progressive dysfunction of photoreceptor and/or retinal pigment epithelium (RPE) cells. In this study, we aimed to evaluate and compare the therapeutic effects of two pluripotent stem cell (PSC)-based therapies. We differentiated RPE from human embryonic stem cells (hESCs) or human-induced pluripotent stem cells (hiPSCs) and transplanted them into the subretinal space of the Royal College of Surgeons (RCS) rat. Once differentiated, cells from either source of PSC resembled mature RPE in their morphology and gene expression profile. Following transplantation, both hESC- and hiPSC-derived cells maintained the expression of specific RPE markers, lost their proliferative capacity, established tight junctions, and were able to perform phagocytosis of photoreceptor outer segments. Remarkably, grafted areas showed increased numbers of photoreceptor nuclei and outer segment disk membranes. Regardless of the cell source, human transplants protected retina from cell apoptosis, glial stress and accumulation of autofluorescence, and responded better to light stimuli. Altogether, our results show that hESC- and hiPSC-derived cells survived, migrated, integrated, and functioned as RPE in the RCS rat retina, providing preclinical evidence that either PSC source could be of potential benefit for treating RD

Role of shiga toxins in cytotoxicity and immunomodulatory effects of escherichia coli O157:H7 during host-bacterial interactions in vitro

Enterohemorrhagic Escherichia coli (EHEC) strains are food-borne pathogens that can cause different clinical conditions. Shiga toxin 2a and/or 2c (Stx2)-producing E. coli O157:H7 is the serotype most frequently associated with severe human disease. In this work we analyzed the hypothesis that host cells participate in Stx2 production, cell damage, and inflammation during EHEC infection. With this aim, macrophage-differentiated THP-1 cells and the intestinal epithelial cell line HCT-8 were incubated with E. coli O157:H7. A time course analysis of cellular and bacterial survival, Stx2 production, stx2 transcription, and cytokine secretion were analyzed in both human cell lines. We demonstrated that macrophages are able to internalize and kill EHEC. Simultaneously, Stx2 produced by internalized bacteria played a major role in macrophage death. In contrast, HCT-8 cells were completely resistant to EHEC infection. Besides, macrophages and HCT-8 infected cells produce IL-1β and IL-8 inflammatory cytokines, respectively. At the same time, bacterial stx2-specific transcripts were detected only in macrophages after EHEC infection. The interplay between bacteria and host cells led to Stx production, triggering of inflammatory response and cell damage, all of which could contribute to a severe outcome after EHEC infections.Fil: Bruballa, Andrea Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Shiromizu, Carolina Maiumi. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Bernal, Alan Mauro. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Pineda, Gonzalo Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Sabbione, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Trevani, Analía Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Bentancor, Leticia Verónica. Universidad Nacional de Quilmes; ArgentinaFil: Oyuela Ramos, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Fernández Brando, Romina Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Muñoz, Manuel Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaFil: Palermo, Marina Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentin