Artificial intelligent system for multimedia services in smart home environments

[EN] Internet of Things (IoT) has introduced new applications and environments. Smart Home provides new ways of communication and service consumption. In addition, Artificial Intelligence (AI) and deep learning have improved different services and tasks by automatizing them. In this field, reinforcement learning (RL) provides an unsupervised way to learn from the environment. In this paper, a new intelligent system based on RL and deep learning is proposed for Smart Home environments to guarantee good levels of QoE, focused on multimedia services. This system is aimed to reduce the impact on user experience when the classifying system achieves a low accuracy. The experiments performed show that the deep learning model proposed achieves better accuracy than the KNN algorithm and that the RL system increases the QoE of the user up to 3.8 on a scale of 10.This work has been partially supported by the "Ministerio de Economia y Competitividad" in the "Programa Estatal de Fomento de la Investigacion Cientifica y Tecnica de Excelencia, Subprograma Estatal de Generacion de Conocimiento" within the project under Grant TIN2017-84802-C2-1-P. This work has also been partially founded by the Universitat Polite`cnica de Vale`ncia through the postdoctoral PAID-10-20 program.Rego Mañez, A.; Gonzalez Ramirez, PL.; Jimenez, JM.; Lloret, J. (2022). Artificial intelligent system for multimedia services in smart home environments. Cluster Computing. 25(3):2085-2105. https://doi.org/10.1007/s10586-021-03350-zS2085210525

Human Pleural Fluid Elicits Pyruvate and Phenylalanine Metabolism in Acinetobacter baumannii to Enhance Cytotoxicity and Immune Evasion

The CCAAT box-harboring proteins represent a family of heterotrimeric transcription factors which is highly conserved in eukaryotes. In fungi, one of the particularly important homologs of this family is the Hap complex that separates the DNA-binding domain from the activation domain and imposes essential impacts on regulation of a wide range of cellular functions. So far, a comprehensive summary of this complex has been described in filamentous fungi but not in the yeast. In this review, we summarize a number of studies related to the structure and assembly mode of the Hap complex in a list of representative yeasts. Furthermore, we emphasize recent advances in understanding the regulatory functions of this complex, with a special focus on its role in regulating respiration, production of reactive oxygen species (ROS) and iron homeostasis.Fil: Nyah, Rodman. California State University; Estados UnidosFil: Martinez, Jasmine. California State University; Estados UnidosFil: Fung, Sammie. California State University; Estados UnidosFil: Nakanouchi, Jun. California State University; Estados UnidosFil: Myers, Amber L.. California State University; Estados UnidosFil: Harris, Caitlin M.. California State University; Estados UnidosFil: Dang, Emily. California State University; Estados UnidosFil: Fernandez, Jennifer. California State University; Estados UnidosFil: Liu, Christine. California State University; Estados UnidosFil: Mendoza, Anthony M.. California State University; Estados UnidosFil: Jimenez, Verónica. California State University; Estados UnidosFil: Nikolaidis, Nikolas. California State University; Estados UnidosFil: Brennan, Catherine A.. California State University; Estados UnidosFil: Bonomo, Robert A.. Louis Stokes Cleveland Department of Veterans Affairs Medical Cente; Estados Unidos. Center for Antimicrobial Resistance and Epidemiology; Estados Unidos. Case Western Reserve University School of Medicine; Estados UnidosFil: Sieira, Rodrigo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Ramirez, Maria Soledad. California State University; Estados Unido

Transcriptome Metabolic Characterization of Tuber borchii SP1—A New Spanish Strain for In Vitro Studies of the Bianchetto Truffle

Truffles are ascomycete hypogeous fungi belonging to the Tuberaceae family of the Pezizales order that grow in ectomycorrhizal symbiosis with tree roots, and they are known for their peculiar aromas and flavors. The axenic culture of truffle mycelium is problematic because it is not possible in many cases, and the growth rate is meager when it is possible. This limitation has prompted searching and characterizing new strains that can be handled in laboratory conditions for basic and applied studies. In this work, a new strain of Tuber borchii (strain SP1) was isolated and cultured, and its transcriptome was analyzed under different in vitro culture conditions. The results showed that the highest growth of T. borchii SP1 was obtained using maltose-enriched cultures made with soft-agar and in static submerged cultures made at 22 °C. We analyzed the transcriptome of this strain cultured in different media to establish a framework for future comparative studies, paying particular attention to the central metabolic pathways, principal secondary metabolite gene clusters, and the genes involved in producing volatile aromatic compounds (VOCs). The results showed a transcription signal for around 80% of the annotated genes. In contrast, most of the transcription effort was concentrated on a limited number of genes (20% of genes account for 80% of the transcription), and the transcription profile of the central metabolism genes was similar in the different conditions analyzed. The gene expression profile suggests that T. borchii uses fermentative rather than respiratory metabolism in these cultures, even in aerobic conditions. Finally, there was a reduced expression of genes belonging to secondary metabolite clusters, whereas there was a significative transcription of those involved in producing volatile aromatic compounds

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Effectiveness of Ledipasvir/Sofosbuvir with/without Ribavarin in Liver Transplant Recipients with Hepatitis C.

Background and Aims: Recurrent infection of hepatitis C virus (HCV) in liver transplant (LT) recipients is universal and associated with significant morbidity and mortality. Methods: We retrospectively evaluated the safety and efficacy of ledipasvir/sofosbuvir with and without ribavirin in LT recipients with recurrent genotype 1 hepatitis C. Results: Eighty-five LT recipients were treated for recurrent HCV with ledipasvir/sofosbuvirwith and without ribavirin for 12 or 24 weeks. The mean (± standard deviation [SD]) time from LT to treatment initiation was 68 (±71) months. The mean (± SD) age of the cohort was 63 (±8.6) years old. Most recipients were male (70%). Baseline alanine transaminase, total bilirubin, and HCV ribonucleic acid (RNA) values (± SD) were 76.8 (±126) mg/dL, 0.8 (±1.3) U/L, and 8,010,421.9 (±12,420,985) IU/mL, respectively. Five of 43 recipients who were treated with ribavirin required drug cessation due to side effects, with 4 of those being anemia complications. No recipient discontinued the ledipasvir/sofosbuvir. Eighty-one percent of recipients had undetectable viral levels at 4 weeks after starting therapy, and all recipients had complete viral suppression at the end of therapy. The sustained viral response at 12 weeks after completion of therapy was 94%. Conclusion : Ledipasvir and sofosbuvir with and without ribavirin therapy is an effective and well-tolerated interferon-free treatment for recurrent HCV infection after LT. Anemia is not uncommon in LT recipients receiving ribavirin

Momentos críticos del partido en las mejores ligas europeas de fútbol sala

El presente estudio pretende determinar los momentos críticos donde se consigue el mayor número de goles en la competición de fútbol sala estableciendo diferencias entre las mejores ligas europeas según títulos conseguidos. Material y método: se trata de un estudio cuantitativo, descriptivo y comparativo entre las ligas española, italiana y rusa durante la temporada 2014-2015. La muestra asciende a la totalidad de la población objeto de estudio, que corresponde a 110 partidos en la liga italiana (11 equipos), 240 en la liga española (16 equipos) y 273 en la liga rusa (14 equipos), lo que supone un total de 623 partidos de los que se han podido analizar 619 y una muestra (n) alcanzada de 4716 goles, de los que se han podido analizar 4468. Para el registro de los goles se ha dividido la variable tiempo en intervalos de 5 minutos. Se há utilizado uma estadística descriptiva e inferencial en la búsqueda de relaciones asociativas entre variables categóricas. Resultados: se obtiene que los momentos con más goles del partido son los últimos 10 minutos de la segunda parte y los últimos 5 minutos de la primera parte. Además se determina que las tres mejores ligas europeas se comportan de la misma manera no encontrando diferencias significativas entre ellas. Estos resultados proporcionan información útil sobre las características de los partidos que ayudan a entender mejor la lógica interna de este deporte. The presented research is a quantitative, descriptive and comparative study whose objective is to determine the critical moments of the futsal match by dividing the time variable up to 5 minute intervals, establishing if there are differences between the best European leagues according to titles obtained; Spanish, Italian and Russian league, during the 2014-2015 season. The sample amounts to the entire population under study, corresponding to 110 matches in the Italian league (11 teams), 240 in the Spanish league (16 teams) and 273 in the Russian league (14 teams), which means a total of 623 matches of which 619 have been analyzed and a sample (n) reached of 4716 goals, of which 4468 have been analyzed. The results obtained establish that the moments with the most goals of the game are the last 10 minutes of the second part and the last 5 minutes of the first part. In addition it is determined that the three best European leagues behave in the same way, not finding significant differences between them

Momentos críticos del partido en las mejores ligas europeas de fútbol sala

El presente estudio pretende determinar los momentos críticos donde se consigue el mayor número de goles en la competición de fútbol sala estableciendo diferencias entre las mejores ligas europeas según títulos conseguidos. Material y método: se trata de un estudio cuantitativo, descriptivo y comparativo entre las ligas española, italiana y rusa durante la temporada 2014-2015. La muestra asciende a la totalidad de la población objeto de estudio, que corresponde a 110 partidos en la liga italiana (11 equipos), 240 en la liga española (16 equipos) y 273 en la liga rusa (14 equipos), lo que supone un total de 623 partidos de los que se han podido analizar 619 y una muestra (n) alcanzada de 4716 goles, de los que se han podido analizar 4468. Para el registro de los goles se ha dividido la variable tiempo en intervalos de 5 minutos. Se há utilizado uma estadística descriptiva e inferencial en la búsqueda de relaciones asociativas entre variables categóricas. Resultados: se obtiene que los momentos con más goles del partido son los últimos 10 minutos de la segunda parte y los últimos 5 minutos de la primera parte. Además se determina que las tres mejores ligas europeas se comportan de la misma manera no encontrando diferencias significativas entre ellas. Estos resultados proporcionan información útil sobre las características de los partidos que ayudan a entender mejor la lógica interna de este deporte. The presented research is a quantitative, descriptive and comparative study whose objective is to determine the critical moments of the futsal match by dividing the time variable up to 5 minute intervals, establishing if there are differences between the best European leagues according to titles obtained; Spanish, Italian and Russian league, during the 2014-2015 season. The sample amounts to the entire population under study, corresponding to 110 matches in the Italian league (11 teams), 240 in the Spanish league (16 teams) and 273 in the Russian league (14 teams), which means a total of 623 matches of which 619 have been analyzed and a sample (n) reached of 4716 goals, of which 4468 have been analyzed. The results obtained establish that the moments with the most goals of the game are the last 10 minutes of the second part and the last 5 minutes of the first part. In addition it is determined that the three best European leagues behave in the same way, not finding significant differences between them

Growth Factor Binding Peptides in Poly (Ethylene Glycol) Diacrylate (PEGDA)-Based Hydrogels for an Improved Healing Response of Human Dermal Fibroblasts

Growth factors (GF) are critical cytokines in wound healing. However, the direct delivery of these biochemical cues into a wound site significantly increases the cost of wound dressings and can lead to a strong immunological response due to the introduction of a foreign source of GFs. To overcome this challenge, we designed a poly(ethylene glycol) diacrylate (PEGDA) hydrogel with the potential capacity to sequester autologous GFs directly from the wound site. We demonstrated that synthetic peptide sequences covalently tethered to PEGDA hydrogels physically retained human transforming growth factor beta 1 (hTGFβ1) and human vascular endothelial growth factor (hVEGF) at 3.2 and 0.6 ng/mm2, respectively. In addition, we demonstrated that retained hTGFβ1 and hVEGF enhanced human dermal fibroblasts (HDFa) average cell surface area and proliferation, respectively, and that exposure to both GFs resulted in up to 1.9-fold higher fraction of area covered relative to the control. After five days in culture, relative to the control surface, non-covalently bound hTGFβ1 significantly increased the expression of collagen type I and hTGFβ1 and downregulated vimentin and matrix metalloproteinase 1 expression. Cumulatively, the response of HDFa to hTGFβ1 aligns well with the expected response of fibroblasts during the early stages of wound healing

Methods for interpreting lists of affected genes obstained in a DNA microarray experiment

Background - The aim of this paper was to describe and compare the methods used and the results obtained by the participants in a joint EADGENE (European Animal Disease Genomic Network of Excellence) and SABRE (Cutting Edge Genomics for Sustainable Animal Breeding) workshop focusing on post analysis of microarray data. The participating groups were provided with identical lists of microarray probes, including test statistics for three different contrasts, and the normalised log-ratios for each array, to be used as the starting point for interpreting the affected probes. The data originated from a microarray experiment conducted to study the host reactions in broilers occurring shortly after a secondary challenge with either a homologous or heterologous species of Eimeria. Results - Several conceptually different analytical approaches, using both commercial and public available software, were applied by the participating groups. The following tools were used: Ingenuity Pathway Analysis, MAPPFinder, LIMMA, GOstats, GOEAST, GOTM, Globaltest, TopGO, ArrayUnlock, Pathway Studio, GIST and AnnotationDbi. The main focus of the approaches was to utilise the relation between probes/genes and their gene ontology and pathways to interpret the affected probes/genes. The lack of a well-annotated chicken genome did though limit the possibilities to fully explore the tools. The main results from these analyses showed that the biological interpretation is highly dependent on the statistical method used but that some common biological conclusions could be reached. Conclusion - It is highly recommended to test different analytical methods on the same data set and compare the results to obtain a reliable biological interpretation of the affected genes in a DNA microarray experimen

MYT1 role in the microtia-craniofacial microsomia spectrum

Q2Background: Craniofacial microsomia (CFM), also known as the oculo-auriculovertebral spectrum, comprises a variable phenotype with the most common features including microtia and mandibular hypoplasia on one or both sides, in addition to lateral oral clefts, epibulbar dermoids, cardiac, vertebral, and renal abnormalities. The etiology of CFM is largely unknown. The MYT1 gene has been reported as a candidate based in mutations found in three unrelated individuals. Additional patients with mutations in this gene are required to establish its causality. We present two individuals with CFM that have rare variants in MYT1 contributing to better understand the genotype and phenotype associated with mutations in this gene. Methods/Results: We conducted genetic analysis using whole-exome and -genome sequencing in 128 trios with CFM. Two novel MYT1 mutations were identified in two participants. Sanger sequencing was used to confirm these mutations. Conclusion: We identified two additional individuals with CFM who carry rare variants in MYT1, further supporting the presumptive role of this gene in the CFM spectrum.N/